Difference between revisions of "Team:Tufts/Safety"

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<h2> Safety </h2>
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<h3> Commitment to Safety </h3>
  
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<h1> Safety </h1>
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<p>Tufts Synthetic Biology promotes health, safety, and environmental protection in teaching, research, and public service by working closely with Tufts Environmental Health and Safety (TEHS) to ensure all members receive necessary training. TEHS provides leadership in developing and supporting high quality programs that allow our students to protect themselves from potential health hazards they could encounter. Tufts Synthetic Biology focuses on working with biological materials in a way that is safe for both researchers and the environment. In all cases, the researcher, lecturer, or third party is consciously aware of potential hazardous effects and has all necessary knowledge to do so safely, while meeting applicable laws and regulations.</p>
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<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h3> Local Rules and Regulations </h3>
  
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<p>The project was completed on the Tufts University Medford campus, overseen by the Tufts Office of Environmental Health and Safety (Tufts EH & S) and the Institutional Biosafety Committee (IBC). The biosafety guidelines of our institution can be found at the following link: https://publicsafety.tufts.edu/ehs/laboratory-safety/ We submitted an IBC form to alert the University of the project we were pursuing, the location of our labs, and all the relevant details of how we would carry out the research. Our lab was inspected and the project was approved. All of our team members have received and passed the Biosafety in Research Laboratories training. </p>
  
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<h3> Project Risks to the Researcher, Public and Environment </h3>
  
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<p>Since we were working with only DNA, RNA, in-vitro translation kits, and in-vitro transcription kits, few reagents posed any danger to researchers or anyone in the facility overall. As we used some ethidium bromide for gel staining early on, we were careful to use gloves when preparing it, and disposed of gels in a sealed liquid waste bucket. Later on we modified the protocols for gel electrophoresis to use SYBR-Safe, which is a non-carcinogenic DNA stain. Aside from that, we worked with e-coli in the interlab study and made sure to handle the bacteria with gloves and dispose of any waste in bio-hazardous waste containers.</p>
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<h3>Safe Project Design</h3>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h3>Safe Lab Work</h3>
 
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
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<h3>Safe Shipment</h3>
 
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
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Latest revision as of 02:42, 17 October 2018

HOME
TEAM MEMBERS
PROJECT
Description
Design
Experiments
Notebook
InterLab
Results
Attributions
SAFETY
HUMAN PRACTICES
Human Practices
Education & Engagement
AWARDS
JUDGING FORM ⇗

Safety

Commitment to Safety

Tufts Synthetic Biology promotes health, safety, and environmental protection in teaching, research, and public service by working closely with Tufts Environmental Health and Safety (TEHS) to ensure all members receive necessary training. TEHS provides leadership in developing and supporting high quality programs that allow our students to protect themselves from potential health hazards they could encounter. Tufts Synthetic Biology focuses on working with biological materials in a way that is safe for both researchers and the environment. In all cases, the researcher, lecturer, or third party is consciously aware of potential hazardous effects and has all necessary knowledge to do so safely, while meeting applicable laws and regulations.

Local Rules and Regulations

The project was completed on the Tufts University Medford campus, overseen by the Tufts Office of Environmental Health and Safety (Tufts EH & S) and the Institutional Biosafety Committee (IBC). The biosafety guidelines of our institution can be found at the following link: https://publicsafety.tufts.edu/ehs/laboratory-safety/ We submitted an IBC form to alert the University of the project we were pursuing, the location of our labs, and all the relevant details of how we would carry out the research. Our lab was inspected and the project was approved. All of our team members have received and passed the Biosafety in Research Laboratories training.

Project Risks to the Researcher, Public and Environment

Since we were working with only DNA, RNA, in-vitro translation kits, and in-vitro transcription kits, few reagents posed any danger to researchers or anyone in the facility overall. As we used some ethidium bromide for gel staining early on, we were careful to use gloves when preparing it, and disposed of gels in a sealed liquid waste bucket. Later on we modified the protocols for gel electrophoresis to use SYBR-Safe, which is a non-carcinogenic DNA stain. Aside from that, we worked with e-coli in the interlab study and made sure to handle the bacteria with gloves and dispose of any waste in bio-hazardous waste containers.