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Modeling

Droplet Analysis
XY Translational Stage

Droplet Characterization

Due to the relatively low flow rate that fluid moves at in microfluidic chips, outputs offloaded from microfluidic chips typically form droplets as opposed to a continuous stream. Therefore, in order to accurately dispense these droplets into wells, we created a model to predict their volume so that users can control the end sample volume in the well of interest. To develop the model we used a free-body diagram to identify the forces present right before a droplet falls off the nozzle. Using the sum of the forces we were able to model the volume of the droplet. To test the model we ran preliminary experiments to calculate volumes and compared them against the theoretical value given by the model. Because we can’t directly measuring the volume of the droplet, we used the time interval between droplets and the given flow rate to calculate what the experimental volume is.

After comparing the experimental droplet volume to the theoretical droplet volume value at flow rates of 1 mL/hr, 2.5 mL/hr, 5 mL/hr, 7.5 mL/hr we learned two things:

the theoretical volume from the model was less than the experimental
this difference, on average, increased with flow rate

The droplet volumes was expected to be consistent across all flow rates and only change depending on tubing geometry and material because as flow rates increase, the interval between droplets decrease as the volume of the droplets should remain constant. We concluded that the model was missing another physical component that would both contribute to the theoretical droplet volume and account for the dependence on flow rate.

A potential reason for this missing component is that the model assumed that the fluid is static instead of dynamic, due to the constant flow rate of fluid moving through the chip (1). In a dynamic model, the total droplet volume is characterized by the static volume and the pinching volume (1). The pinching volume is formed during the droplet’s fall when the droplet breaks off the tube (1).

However, after researching the physical phenomenon behind dynamic droplets we realized that a complete dynamic model, as described in Zhang et al, required measuring many parameters, making it difficult to implement 1. Therefore, we decided to create our dynamic model empirically by adding a correction factor, which would depend on flow rate.

Zhang et al.

In order to determine the correction factor we ran a series of experiments to calculate experimental droplet sizes at 15 different flow rates. For statistical significance we measured 30 droplet volumes per flow rate by measuring the time interval between droplets. Using a MATLAB script, the droplet volumes were used to calculate the correction factor for each flow rate. The average correction factor was then calculated to see how they differed between flow rates. The plots blow display the results of our modeling experiments.

Figure 1. Plot of the average experimental droplet volume versus the predicted volume from the static model.

Figure 2. Plot of the correction factors calculate based on the experimental volumes at various flow rates against the average correction factor.

Unfortunately, these experiments did not result in a consistent correction factor that can be used across different flow rates. While we observed an increase in droplet size as flow rate increased, we concluded that either more data was necessary to extrapolate an experimental correction factor or more However, we learned other important information to adjust the current static model. Outside of the 2.5 mL/hr trial, the experimental droplet volumes have low standard deviates and are centered around the average volume size. Because of this we decided to multiply the static model by a factor of 1.33 to account for the difference between average and static model.

References:

Zhang, D. F., and H. A. Stone. “Drop Formation in Viscous Flows at a Vertical Capillary Tube.” Physics of Fluids 9, no. 8 (August 1997): 2234–42. https://doi.org/10.1063/1.869346.

Distance Traveled to Dispense Fluid

In order to accurately dispense the output into the desired wells, the plane needed to travel the right amount of steps depending on the well plate. Therefore we needed to calculate how many steps of the stepper motor is required to move from one well to another. Below are the steps and calculations that went into determining this:

To do so, we first determined the distance from center of well to next center well for each microtiter plate.

Next, we measured the circumference of our pulley.

After obtaining these values, we found the distance it is possible to travel per step, and finally the steps per well. From the documentation for the NEMA-14 stepper motor, we know it steps 200 times in one revolution and has microstepping features up to 1/32 microsteps.

From there, since we know how many steps is required for 96 wells, we calculate the microtiter plates with different amounts of wells.

Well Plate	Dimensions	Steps/Well
24-well plate	4x6 wells (18 mm apart)	90 steps
96-well plate	8x12 wells (9 mm apart)	45 steps
384-well plate	16x24 wells (4.5 mm apart)	22.5 steps

Runs Before Failure

To characterize the amount of runs the XY-stage can perform before failure, we ran TERRA through a set of 15 trials in which fluid was dispensed into two different wells ten times. These trials were run independently from each other by resetting TERRA in between trials. In each trial, two outputs were simulated--one outputting at location 45, and the latter outputting at location 90. The experiments allowed us to understand how many loops TERRA would run through before it fails to output into a correct well location, and therefore requires a reset.

Terra averages around 8 runs before it requires a reset. A reset consists of rehoming the device from its starting point, readjusting the tubing, and resetting the plate on the plane.

Speed and Time

A set of 15 trials were run in order to determine the speed of the plate support of the XY-stage. The plate support traveled a distance of 108mm. The time taken to travel was recorded for each trial and then averaged. To calculate the average speed, the distance traveled was divided by the time per run.

The average time taken to transverse the 108 mm was 39.306 seconds. From that, we determined that, with a microstep of 1/4 steps, the XY-stage moves at an average speed of 2.75 mm/sec. The average time taken to travel between wells on a 96 well plate was 3.28 seconds.