Difference between revisions of "Team:Marburg/Demonstrate"

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<p>Realizing V. natriegens as a widely used host organism for synthetic biology requires well funded knowledge about it! Realizing this, we prioritised fundamental  research early on. We showed the unparalleled speed of V. natriegens replication, defined a range of optimal growth conditions, including pH and salt tolerance, and demonstrated the ease of its genetic accessibility.  <br> </p>
 
 
<p>
 
<p>
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We managed  to enable transformation protocols for with high electroporation efficiency and heat-shock transformation to drive synthetic biology research. <br>
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Realizing <i>V. natriegens</i> as a widely used host organism for synthetic biology requires well-funded knowledge about it! Realizing this, we prioritized fundamental research early on. We showed the unparalleled speed of <i>V. natriegens</i> replication, defined a range of optimal growth conditions, including pH and salt tolerance, and the ease of its genetic accessibility.
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</p> <p>
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In combination with our Marburg Toolbox, we accomplished cloning of  simple plasmids in under 12 hours, and assembly and preparation of level 2 golden gate constructs in under three days!<br>
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</p> <p>
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Additionally, our team successfully implemented Gibson and Aqua cloning and achieved high reliability at high performances.  
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We sequenced both chromosomes with Illumina sequencing , mapped them to existing genome maps and ran automated annotation tools to identify genetic features.
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<br></p> <p>
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Working concentrations for most common antibiotics were elucidated and used throughout the project.
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<br></p> <p>
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Applying several electron microscopic methods, we could, apart from generating nice pictures, highlight shape, form and volume of V. natriegens. Fortunately, we could observe several cell divisions in mid process.
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</p>
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<a href="https://2018.igem.org/Team:Marburg/Results"><abbr title="Link to the results page">  </abbr> protocols </a>
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</p>
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<p>
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We managed to enable transformation <a href="https://2018.igem.org/Team:Marburg/Experiments"><abbr title="Link to the experiment page">  </abbr> demonstrated </a>
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for with high electroporation efficiency and heat-shock transformation to drive synthetic biology research.
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</p>
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<p>
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In combination with our 
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<a href="https://2018.igem.org/Team:Marburg/Part_Collection"><abbr title="Link to the part collection page">Marburg-Collection,</abbr></a>
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we accomplished cloning of simple plasmids, from transformation to miniprep, under 12 hours, and assembly and preparation of level 2 golden gate constructs in under three days!
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</p>
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<p>
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Additionally, our team successfully implemented five fragment <a href="https://2018.igem.org/Team:Marburg/Results"><abbr title="Link to the results page">Gibson cloning </abbr></a> as well as <a href="https://2018.igem.org/Team:Marburg/Results"><abbr title="Link to the results page">Aquand cloning </abbr></a>
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            and achieved high reliability at high performances.
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</p>
 +
 +
<p>
 +
We sequenced both chromosomes with Illumina sequencing, mapped them to existing genome maps and ran automated annotation tools to identify genetic features.
 +
</p>
 +
 +
<p>
 +
Working concentrations for most common antibiotics were elucidated and used throughout the project.
 +
</p>
 +
 +
<p>
 +
Applying several electron microscopic methods, we could, apart from generating
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<a href="https://2018.igem.org/File:T--Marburg--Josef_1.png"><abbr title="Link to a EM picture of V. natriegens">nice pictures,</abbr></a>
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  highlight shape, form and volume of <i>V. natriegens. </i>Fortunately, we could observe several cell divisions in mid process.
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</p>
  
  

Revision as of 08:38, 17 October 2018

Description

Vibrio Basics

Realizing V. natriegens as a widely used host organism for synthetic biology requires well-funded knowledge about it! Realizing this, we prioritized fundamental research early on. We showed the unparalleled speed of V. natriegens replication, defined a range of optimal growth conditions, including pH and salt tolerance, and the ease of its genetic accessibility. protocols

We managed to enable transformation demonstrated for with high electroporation efficiency and heat-shock transformation to drive synthetic biology research.

In combination with our Marburg-Collection, we accomplished cloning of simple plasmids, from transformation to miniprep, under 12 hours, and assembly and preparation of level 2 golden gate constructs in under three days!

Additionally, our team successfully implemented five fragment Gibson cloning as well as Aquand cloning and achieved high reliability at high performances.

We sequenced both chromosomes with Illumina sequencing, mapped them to existing genome maps and ran automated annotation tools to identify genetic features.

Working concentrations for most common antibiotics were elucidated and used throughout the project.

Applying several electron microscopic methods, we could, apart from generating nice pictures, highlight shape, form and volume of V. natriegens. Fortunately, we could observe several cell divisions in mid process.