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<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Comparison between PSB">Comparison between PSB</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Comparison between PSB">Comparison between PSB</a></li> | ||
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Sort of three genes">Sort of three genes</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Sort of three genes">Sort of three genes</a></li> | ||
− | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/ | + | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Software">Intelligent device software</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
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<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Parts Overview">Parts Overview</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Parts Overview">Parts Overview</a></li> | ||
− | |||
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Composite part">Composite part</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Composite part">Composite part</a></li> | ||
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Part collection">Part collection</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Part collection">Part collection</a></li> | ||
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<div class="row"> | <div class="row"> | ||
<div class="col-lg-12"> | <div class="col-lg-12"> | ||
− | <h2 class="pageTitle">Parts Overview | + | <h2 class="pageTitle">Parts Overview</h2> |
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
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<div class="container content"> | <div class="container content"> | ||
<div class="row"> | <div class="row"> | ||
− | |||
<div class="col-md-12 content-text"> | <div class="col-md-12 content-text"> | ||
<div class="about-logo"> | <div class="about-logo"> | ||
− | + | <p>As mentioned in the project description and result, our three different circuits in three different kinds of bacteria well supported our project and each of them contributes a lot to the biobricks. This year, we designed, constructed and verified 25 parts, and 21 of them were submitted. There are <a href="https://2018.igem.org/Team:HUST-China/Composite_part" style="color:blue;">composite parts</a> producing enzyme to transform different carbon source to lactate and transform out of the bacteria. We completely collected the parts from our project and well documented them with our test data.</p> | |
− | <p | + | |
− | This year, we designed, constructed and verified 25 parts, and 21 of them were submitted. There are | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
</div> | </div> | ||
− | <div class="col-md- | + | <div class="col-md-10 col-md-offset-1 table-responsive"> |
<table class="table table-bordered table-hover" style="text-align: center;"> | <table class="table table-bordered table-hover" style="text-align: center;"> | ||
<tr> | <tr> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533000</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533000" style="color:blue;">BBa_K2533000</a></td> |
<td>RBS-ldhD-RBS-lldP</td> | <td>RBS-ldhD-RBS-lldP</td> | ||
<td>dhD can produce lactate, and lldP can release it from cytoplasm to the environment</td> | <td>dhD can produce lactate, and lldP can release it from cytoplasm to the environment</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533002</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533001" style="color:blue;">BBa_K2533001</td> |
+ | <td>RBS-ldhD-FLAG-lldP-his tag</td> | ||
+ | <td>ldhD can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533002" style="color:blue;">BBa_K2533002</a></td> | ||
<td>RBS-ldhDnARSdR-RBS-lldP</td> | <td>RBS-ldhDnARSdR-RBS-lldP</td> | ||
<td>ldhDnARSdR can produce lactate, and lldP can release it from cytoplasm to the environment.</td> | <td>ldhDnARSdR can produce lactate, and lldP can release it from cytoplasm to the environment.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533003</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533003" style="color:blue;">BBa_K2533003</a></td> |
<td>RBS-ldhDARSdR-RBS-lldP</td> | <td>RBS-ldhDARSdR-RBS-lldP</td> | ||
<td>ldhDARSdR can produce lactate, and lldP can release it from cytoplasm to the environment.</td> | <td>ldhDARSdR can produce lactate, and lldP can release it from cytoplasm to the environment.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533014</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533014" style="color:blue;">BBa_K2533014</a></td> |
<td>RBS-TH-RBS-gldA-RBS-LLDP</td> | <td>RBS-TH-RBS-gldA-RBS-LLDP</td> | ||
<td>produce and release lactate</td> | <td>produce and release lactate</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533016" style="color:blue;">BBa_K2533016</a></td> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
<td>RBS-ldhD-FLAG-lldP-his tag</td> | <td>RBS-ldhD-FLAG-lldP-his tag</td> | ||
<td>ldhD can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | <td>ldhD can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533017</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533017" style="color:blue;">BBa_K2533017</a></td> |
<td>RBS-ldhDC-FLAG-lldP-his tag</td> | <td>RBS-ldhDC-FLAG-lldP-his tag</td> | ||
<td>ldhDC can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | <td>ldhDC can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533018</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533018" style="color:blue;">BBa_K2533018</a></td> |
<td>RBS-ldhDnARSdR-FLAG-RBS-lldP-his tag</td> | <td>RBS-ldhDnARSdR-FLAG-RBS-lldP-his tag</td> | ||
<td>ldhDnARSdR can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | <td>ldhDnARSdR can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533019</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533019" style="color:blue;">BBa_K2533019</a></td> |
<td>RBS-ldhDARSdR-FLAG-RBS-lldP-his tag</td> | <td>RBS-ldhDARSdR-FLAG-RBS-lldP-his tag</td> | ||
<td>ldhDARSdR can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | <td>ldhDARSdR can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533030</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533030" style="color:blue;">BBa_K2533030</a></td> |
<td>RBS-dld</td> | <td>RBS-dld</td> | ||
<td>It encodes D-lactate dehydrogenase.</td> | <td>It encodes D-lactate dehydrogenase.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533031</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533031" style="color:blue;">BBa_K2533031</a></td> |
<td>RBS-lldEFG</td> | <td>RBS-lldEFG</td> | ||
<td>produce L-lactate dehydrogenase complex protein.</td> | <td>produce L-lactate dehydrogenase complex protein.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533032</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533032" style="color:blue;">BBa_K2533032</a></td> |
<td>RBS-dld-TT-Ptac-RBS-lldEFG</td> | <td>RBS-dld-TT-Ptac-RBS-lldEFG</td> | ||
<td>It encodes FAD-dependent D-lactate dehydrogenase and L-lactate dehydrogenase complex protein.</td> | <td>It encodes FAD-dependent D-lactate dehydrogenase and L-lactate dehydrogenase complex protein.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533033</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533033" style="color:blue;">BBa_K2533033</a></td> |
<td>RBS-gapA</td> | <td>RBS-gapA</td> | ||
<td>It encodes glyceraldehyde-3-phosphate dehydrogenase.</td> | <td>It encodes glyceraldehyde-3-phosphate dehydrogenase.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533034</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533034" style="color:blue;">BBa_K2533034</a></td> |
<td>RBS-mdh</td> | <td>RBS-mdh</td> | ||
<td>It encodes NAD dependent malate dehydrogenase.</td> | <td>It encodes NAD dependent malate dehydrogenase.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533035</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533035" style="color:blue;">BBa_K2533035</a></td> |
<td>RBS-gapA-RBS-mdh-TT</td> | <td>RBS-gapA-RBS-mdh-TT</td> | ||
<td>It encodes glyceraldehyde-3-phosphate dehydrogenase and NAD dependent malate dehydrogenase.</td> | <td>It encodes glyceraldehyde-3-phosphate dehydrogenase and NAD dependent malate dehydrogenase.</td> | ||
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<tr> | <tr> | ||
− | <td>BBa_K2533036</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533036" style="color:blue;">BBa_K2533036</a></td> |
<td>RBS-pflB</td> | <td>RBS-pflB</td> | ||
<td>It encodes pyruvate formate-lyase.</td> | <td>It encodes pyruvate formate-lyase.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533037</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533037" style="color:blue;">BBa_K2533037</a></td> |
<td>RBS-fdh</td> | <td>RBS-fdh</td> | ||
<td>It encodes formate dehydrogenase cytochrome b.</td> | <td>It encodes formate dehydrogenase cytochrome b.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533038</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533038" style="color:blue;">BBa_K2533038</a></td> |
<td>RBS-pflB-RBS-fdh</td> | <td>RBS-pflB-RBS-fdh</td> | ||
<td>It encodes pyruvate formate-lyase and formate.</td> | <td>It encodes pyruvate formate-lyase and formate.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533039</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533039" style="color:blue;">BBa_K2533039</a></td> |
<td>RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh</td> | <td>RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh</td> | ||
<td>It encodes glyceraldehyde-3-phosphate dehydrogenase, NAD dependent malate dehydrogenase, pyruvate formate-lyase and formate dehydrogenase cytochrome b.</td> | <td>It encodes glyceraldehyde-3-phosphate dehydrogenase, NAD dependent malate dehydrogenase, pyruvate formate-lyase and formate dehydrogenase cytochrome b.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533045</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533045" style="color:blue;">BBa_K2533045</a></td> |
<td>RBS-mleS</td> | <td>RBS-mleS</td> | ||
<td>It encodes malate dehydrogenase.</td> | <td>It encodes malate dehydrogenase.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533046</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533046" style="color:blue;">BBa_K2533046</a></td> |
<td>RBS-lldP</td> | <td>RBS-lldP</td> | ||
<td>It encodes L-lactate permease.</td> | <td>It encodes L-lactate permease.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533047</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533047" style="color:blue;">BBa_K2533047</a></td> |
<td>RBS-ldhA</td> | <td>RBS-ldhA</td> | ||
<td>It encodes D-lactate dehydrogenase.</td> | <td>It encodes D-lactate dehydrogenase.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533048</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533048" style="color:blue;">BBa_K2533048</a></td> |
<td>RBS-mleS-RBS-lldP</td> | <td>RBS-mleS-RBS-lldP</td> | ||
<td>It encodes malate dehydrogenase & L-lactate permease.</td> | <td>It encodes malate dehydrogenase & L-lactate permease.</td> | ||
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<tr> | <tr> | ||
− | <td>BBa_K2533049</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533049" style="color:blue;">BBa_K2533049</a></td> |
<td>RBS-lldP-RBS-ldhA</td> | <td>RBS-lldP-RBS-ldhA</td> | ||
<td>It encodes L-lactate permease & D-lactate dehydrogenase.</td> | <td>It encodes L-lactate permease & D-lactate dehydrogenase.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533050</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533050" style="color:blue;">BBa_K2533050</a></td> |
<td>mleS-lldp-ldhA</td> | <td>mleS-lldp-ldhA</td> | ||
<td>It encodes malate dehydrogenase & L-lactate permease & D-lactate dehydrogenase.</td> | <td>It encodes malate dehydrogenase & L-lactate permease & D-lactate dehydrogenase.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533051</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533051" style="color:blue;">BBa_K2533051</a></td> |
<td>mleS-lldP-TT</td> | <td>mleS-lldP-TT</td> | ||
<td>It encodes malate dehydrogenase & L-lactate permease.</td> | <td>It encodes malate dehydrogenase & L-lactate permease.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533052</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533052" style="color:blue;">BBa_K2533052</a></td> |
<td>RBS-lldP-RBS-ldhA-TT</td> | <td>RBS-lldP-RBS-ldhA-TT</td> | ||
<td>Product and transport of lactic acid.</td> | <td>Product and transport of lactic acid.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533053</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533053" style="color:blue;">BBa_K2533053</a></td> |
<td>RBS-mleS-RBS-lldP-RBS-ldhA-TT</td> | <td>RBS-mleS-RBS-lldP-RBS-ldhA-TT</td> | ||
<td>Product and transport of lactic acid.</td> | <td>Product and transport of lactic acid.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533054</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533054" style="color:blue;">BBa_K2533054</a></td> |
<td>RBS-ldhA-RBS-lldP</td> | <td>RBS-ldhA-RBS-lldP</td> | ||
<td>Product and transport of lactic acid.</td> | <td>Product and transport of lactic acid.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>BBa_K2533055</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533055" style="color:blue;">BBa_K2533055</a></td> |
<td>RBS-ldhA-RBS-lldP-TT</td> | <td>RBS-ldhA-RBS-lldP-TT</td> | ||
<td>Product and transport of lactic acid.</td> | <td>Product and transport of lactic acid.</td> |
Revision as of 09:06, 17 October 2018
Parts Overview
As mentioned in the project description and result, our three different circuits in three different kinds of bacteria well supported our project and each of them contributes a lot to the biobricks. This year, we designed, constructed and verified 25 parts, and 21 of them were submitted. There are composite parts producing enzyme to transform different carbon source to lactate and transform out of the bacteria. We completely collected the parts from our project and well documented them with our test data.
Part Number | Name | Description |
---|---|---|
BBa_K2533000 | RBS-ldhD-RBS-lldP | dhD can produce lactate, and lldP can release it from cytoplasm to the environment |
BBa_K2533001 | RBS-ldhD-FLAG-lldP-his tag | ldhD can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein. |
BBa_K2533002 | RBS-ldhDnARSdR-RBS-lldP | ldhDnARSdR can produce lactate, and lldP can release it from cytoplasm to the environment. |
BBa_K2533003 | RBS-ldhDARSdR-RBS-lldP | ldhDARSdR can produce lactate, and lldP can release it from cytoplasm to the environment. |
BBa_K2533014 | RBS-TH-RBS-gldA-RBS-LLDP | produce and release lactate |
BBa_K2533016 | RBS-ldhD-FLAG-lldP-his tag | ldhD can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein. |
BBa_K2533017 | RBS-ldhDC-FLAG-lldP-his tag | ldhDC can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein. |
BBa_K2533018 | RBS-ldhDnARSdR-FLAG-RBS-lldP-his tag | ldhDnARSdR can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein. |
BBa_K2533019 | RBS-ldhDARSdR-FLAG-RBS-lldP-his tag | ldhDARSdR can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein. |
BBa_K2533030 | RBS-dld | It encodes D-lactate dehydrogenase. |
BBa_K2533031 | RBS-lldEFG | produce L-lactate dehydrogenase complex protein. |
BBa_K2533032 | RBS-dld-TT-Ptac-RBS-lldEFG | It encodes FAD-dependent D-lactate dehydrogenase and L-lactate dehydrogenase complex protein. |
BBa_K2533033 | RBS-gapA | It encodes glyceraldehyde-3-phosphate dehydrogenase. |
BBa_K2533034 | RBS-mdh | It encodes NAD dependent malate dehydrogenase. |
BBa_K2533035 | RBS-gapA-RBS-mdh-TT | It encodes glyceraldehyde-3-phosphate dehydrogenase and NAD dependent malate dehydrogenase. |
BBa_K2533036 | RBS-pflB | It encodes pyruvate formate-lyase. |
BBa_K2533037 | RBS-fdh | It encodes formate dehydrogenase cytochrome b. |
BBa_K2533038 | RBS-pflB-RBS-fdh | It encodes pyruvate formate-lyase and formate. |
BBa_K2533039 | RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh | It encodes glyceraldehyde-3-phosphate dehydrogenase, NAD dependent malate dehydrogenase, pyruvate formate-lyase and formate dehydrogenase cytochrome b. |
BBa_K2533045 | RBS-mleS | It encodes malate dehydrogenase. |
BBa_K2533046 | RBS-lldP | It encodes L-lactate permease. |
BBa_K2533047 | RBS-ldhA | It encodes D-lactate dehydrogenase. |
BBa_K2533048 | RBS-mleS-RBS-lldP | It encodes malate dehydrogenase & L-lactate permease. |
BBa_K2533049 | RBS-lldP-RBS-ldhA | It encodes L-lactate permease & D-lactate dehydrogenase. |
BBa_K2533050 | mleS-lldp-ldhA | It encodes malate dehydrogenase & L-lactate permease & D-lactate dehydrogenase. |
BBa_K2533051 | mleS-lldP-TT | It encodes malate dehydrogenase & L-lactate permease. |
BBa_K2533052 | RBS-lldP-RBS-ldhA-TT | Product and transport of lactic acid. |
BBa_K2533053 | RBS-mleS-RBS-lldP-RBS-ldhA-TT | Product and transport of lactic acid. |
BBa_K2533054 | RBS-ldhA-RBS-lldP | Product and transport of lactic acid. |
BBa_K2533055 | RBS-ldhA-RBS-lldP-TT | Product and transport of lactic acid. |