Difference between revisions of "Team:Peking/Public Engagement"

 
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         <meta name="author" content="Li Jiamian & Wang Yuqing"/>
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                             <li class="menu-4"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Software">Software</a>
 
                             <li class="menu-4"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Software">Software</a>
 
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                            <li class="menu-6"><a class="colapse-menu1" href="https://2018.igem.org/Team:Peking/Human_Practices">Human Practices</a>
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                                <li class="dropdown menu-6"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Human Practices</a>
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                                 <li class="dropdown menu-7"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Achievement</a>
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                                        <li><a href="https://2018.igem.org/Team:Peking/Human_Practices" class="barfont1">Overview</a></li>
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                                        <li><a href="https://2018.igem.org/Team:Peking/Public_Engagement" class="barfont1">Public Engagement</a></li>
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                                 <li class="dropdown menu-7"><a class="dropdown-toggle" data-toggle="dropdown" href="#" >Achevement</a>
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                                         <li><a href="https://2018.igem.org/Team:Peking/Judging_Form" class="barfont1">Judging Form</a></li>
 
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                     <h1>Demonstrate</h1>
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                     <h1>Public Engagement</h1>
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                    <p class="title1" style="text-align:center">In this section, you could see the demonstration.</p>
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                                     <h4><a href="javascript:void(0);" onclick="naver('A')">Overview</a></h4>
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                                     <h4><a href="javascript:void(0);" onclick="naver('B')">Phase&nbsp;Separation</a></h4>
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                                     <h4><a href="https://2018.igem.org/Team:Peking/Human_Practices#C">Public&nbsp;Engagement</a></h4>
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                                     <h4><a href="https://2018.igem.org/Team:Peking/Human_Practices#D">Accessible&nbsp;Instrument</a></h4>
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                                        <li><a href="javascript:void(0);" onclick="naver('B2')">The&nbsp;formation</a></li>
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                                    <h4><a href="javascript:void(0);" onclick="naver('C')">Functional&nbsp;Organelles</a></h4>
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                                    <p>The aim of our project is to build a synthetic organelle based on phase separation as a multifunctional platform. Based on the principle of multivalence and interaction, we fused interactional modules into homo-oligomeric tags (HOtags) to form granules in S. cerevisiae.</p>
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                                     <p>We have built spontaneous and induced synthetic organelles by specific interaction modules, so that we can control the formation process by different ways for demands in biological engineering. Then we characterized the kinetics and properties of synthetic organelles theoretically and experimentally. These results confirm the potential of synthetic organelles in synthetic biology.</p>
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                                     <p><h3>Talking to high school students</h3></p>
 
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                                     <p>It inspired us to propose some specific applications of our synthetic organelles, including organization hub, sensor, and metabolism regulator. We have verified the feasibility of them by loading GFP-nanobody module, NAD+ sensor module and carotene production module to the whole system.</p>
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                                     <p>One of our team members, Guo Fuyu, went to Hutian Middle School in Huaihua, Hunan Province. He introduced systems and synthetic biology to the students and helped them with biology in high school as well. In our view, it is of fundamental significance to provide as much middle school students in second-tier cities in China as possible with access to frontier science, since quality education is definitely as important as examination-oriented education.
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(Figure. 1)
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<div style="text-align: center;"><img src="https://static.igem.org/mediawiki/2018/7/7f/T--Peking--hp11.jpeg">
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<br/>Guo Fuyu talking with middle school students in Hutian Middle School</div>
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                                     <p>We believe that our work has reached the medal requirements of demonstration as we have confirmed that our synthetic organelles can be formed in vivo and deliver a range of functions both for engineering and research due to their amazing properties. The concrete demonstration of the whole platform is shown below. You can see more details of experiments and modeling in our <a href="https://2018.igem.org/Team:Peking/Results"/>Data Page</a> and <a href="https://2018.igem.org/Team:Peking/Model"/>Modeling</a></p><br/><br/><br/>     
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                                     <p>According to a survey in Peking University, freshmen who have had a sense of higher education and sought for their interest in high school, get accustomed to college life and study remarkably faster than those who haven’t. We genuinely hope university students and professors across China can communicate more with high school students and help every single one find his or her interest worth pursuing their whole life as soon as possible. We especially hope that the students in second-tier cities and rural areas get the same chance of quality education as those in megacities.</p>
 
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                                    <div class="ordi">1.</div>
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                                     <h3>Spontaneous and induced synthetic organelles can be formed by phase separation</h3>
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                                     <p><h3>Pre-school scientific education</h3></p>
 
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                                     <p>Our basic system consists of two components of synthetic organelles. Either of them has a specific HOtag to form homo-oligomers. We expect that they are able to form synthetic organelles due to the principles of phase separation. To verify the feasibility of the design, we fused two fluorescence proteins with the two components of synthetic organelles (Figure1.a) so that we can observe the self-organization of components and the formation of granules under fluorescence microscope.</p>
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                                     <p>While the idea to introduce the most cutting-edge science to children in kindergarten may sound outlandish, we can still spend a nice day with them and introduce them to science. Two of our team members did this in the kindergarten attached to Peking University. We designed a series of games with a science background: demonstrating the three phases of water, observing phase separation, constructing a “phase separation” system with magnetic balls, and water drawing. The kids liked these games very much which inspired us a lot.</p>
 
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                                     <p>We used SUMO-SIM interaction module to build a spontaneous organelle. When two components are expressed in yeasts, granules with the two fluorescence proteins can be observed in vivo (Figure1.b). </p>
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                                     <p>It is a big challenge for us to tell the children about basic science, but we’re happy to see them enjoying the games which is also interesting and relaxing for us. We enjoyed the fascination with science, which can cross the boundary of age and life experience.</p>
 
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                                    <p>Meanwhile, by rapamycin induced interaction module, FKBP-Frb, we have built an inducible organelle. We can see granules occurs in yeasts within minutes after adding the inducer.</a> </p>
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    <th>Kids playing marbling paint together</td>
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Figure1.a The basic design of synthetic organelles with florescence reporters. <img src="https://static.igem.org/mediawiki/2018/3/36/T--Peking--Logo.png" style="width:100%;" alt="">（这里可能需要一张cartoon的设计图）
 
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            b, c fluorescence images of spontaneous organelles (SUMO-SIM based) and inducible synthetic organelles (FKBP-Frb based, after adding 10000 nM rapamycin)<br/><br/>
 
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                                     <h3>The formation of organelles has flexible but predictable properties and kinetics in different conditions</h3>
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                                     <p>This activity made us confident about the perspective of broad-based scientific communication, and we realized the we can communicate in both a “meaningful” and “interesting” way, where all the participants are equal and relaxed and the conversation is much more efficient.</p>
 
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                                     <p>Then we combined <a href="https://2018.igem.org/Team:Peking/Phase_Separation_M"/>modeling of phase separation</a> and experiment to research the kinetics of the organelles formation process expecting that a well-characterized system can reach its whole potential in complex applications. </p>
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                                     <p><h3>Documentation of Peking iGEM as enlightenment for beginners</h3></p>
 
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                                     <p>As the model predicts, the concentration of components and the interaction strength affect the kinetics of phase separation. First we controlled the expression levels of components by using several stable or inducible promoters and observe the system's behavior. We found that the formation of organelles happened in specific promoter combinations and can be controlled by inducible promoters. The analysis result does not only fit well with the simulation, but provides potential methods to control the organelles in applications. </p>
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                                     <p>We have built up a WeChat public platform which is a worldwide platform with billions of users for documentation, communication and popularization. To give the future igemers a taste of igem projects and help them learn the basic rudiments of synthetic biology we have reviewed the projects of Peking iGEM in the past 14 years. All these articles are rather approachable and most of them received positive feedbacks. We demonstrate here the articles and hope it may help more people who want to get to know about synthetic biology.</p>
 
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Figure2 (a) Phase diagram of a phase separation system with three components(simulation). To fit our system, the x-axis and the y-axis stands for the two components in the granules. The asymmetry comes from the assumption that the two components have different interactions with water.
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(b) Fluorescence movies of different promoter combinations of FKBP-Frb mediated system after adding rapamycin. Only in specific combinations, synthetic organelles can be formed by phase separation.
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    <th><a href="https://mp.weixin.qq.com/s/AMd0rNq9AQDu02cUu7HvWw"><img src="https://static.igem.org/mediawiki/2018/c/c3/T--Peking--2007.png"></a></th>
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(c) The formation process of SUMO-SIM mediated synthetic organelles can be controlled by inducible promoters. While the expression of Tet07-SIM-mCherry-HoTag6 is induced by dox gradually, the granules will occur abruptly in some time.<br/><br/>
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    <th><a href="https://mp.weixin.qq.com/s/uhbTZHsgPL8b3YPLm_K9kQ"><img src="https://static.igem.org/mediawiki/2018/c/cb/T--Peking--2008.png"></a></th>
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    <th><a href="https://mp.weixin.qq.com/s/dQj9qOFF_kKO_d7QDMsanQ"><img src="https://static.igem.org/mediawiki/2018/1/1b/T--Peking--2009.png"></a></th>
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    <th><a href="https://mp.weixin.qq.com/s/5qaRHr0pBmB0SphxrpDanQ"><img src="https://static.igem.org/mediawiki/2018/7/7f/T--Peking--2010.png"></a></th>
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    <th>2010</td>
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    <th><a href="https://mp.weixin.qq.com/s/O8sPYmyyIwO_evpzao0DhQ"><img src="https://static.igem.org/mediawiki/2018/1/14/T--Peking--2011.png"></a></th>
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    <th><a href="https://mp.weixin.qq.com/s/m-Ttirv-yjhokSnFQa0qMA"><img src="https://static.igem.org/mediawiki/2018/0/08/T--Peking--2013.png"></a></td>
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    <th><a href="https://mp.weixin.qq.com/s/o2e5y0g0luZuYS957KLMJQ"><img src="https://static.igem.org/mediawiki/2018/8/8a/T--Peking--2016.png"></a></td>
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    <th><a href="https://mp.weixin.qq.com/s/VwFxEy7ab46vAF9Q3A9vcg"><img src="https://static.igem.org/mediawiki/2018/f/fe/T--Peking--2017.png"></a></td>
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                                    <p>The strength of interaction modules can be also controlled. In the rapamycin-induced organelle system, changing the concentration of rapamycin will affect the apparent value of K, a parameter reflecting the interaction strength in our model. In a gradient rapamycin-inducing experiment, the delay time from adding inducer to granules formation was found to be shorter when concentration of rapamycin increases. So we have confirmed the influence of two parameters in models and increased the flexibility of our synthetic organelles.</p>
 
 
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Figure3 (a) A simulation of organelle formation process in different interaction strength of components.
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(b) The speed of FKBP-Frb mediated organelle formation increases with the increasing concentration of rapamycin.
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                                     <p>We also tried to characterized other properties, like the liquid-like property of the synthetic organelles, as they may affect the functions. See more details about our characterizations in <a href="https://2018.igem.org/Team:Peking/Phase_Separation_D"/>DataPage Phase separation</a>.</p><br/><br/><br/>
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                                     <p><h3>Popular video about phase separation in biology</h3></p>
 
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                            <div class="texttitle">Functional Organelles
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                                     <p>Since SPOT can form in the cell and be controlled, we go further to consider the functions of SPOT. The functions of SPOT can be descripted in three catalogs: Spatial segmentation, Sensor and metabolic regulation. We verified the spatial segmentation with the condensation of substrates, also we can load the protein we want by fusing it with nanobody. We then verified the sensor with detecting rapamycin and ABA, which shows strong relativity between the concentration and the proportion of yeasts with SPOT. To find the law behind metabolism in the SPOT, we fuse the enzymes that can produce β-carotene into SPOT and measure the difference between with or without SPOT in produce of β-carotene.</p>
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                                     <p>We made a popular video about phase separation in biology to introduce it to more people studying the subject. We posted it on several websites in China and many undergraduates and graduates were introduced to phase separation through our video. We also found it necessary to communicate more about basic knowledge of different disciplines in the area of systems biology. This is arguably the best way in which people can learn about the system they work on and cooperate better with each other.
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<a href="https://www.youtube.com/watch?v=ug9P5koS-xI"><br/>see more</a>
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Figure4 (organization hub)
 
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（phase内和phase外的胡萝卜素生产实验）<br/><br/><br/><br/><br/>
 
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                                    <p>SPOT has been well verified and has various functions. And in the future, this modular system will have great potential in science and practice using. SPOT can change the modules to gain more different properties like diverse inducing method, we can also use it as a platform and then load other protein with some interactions like the interaction between nanobody and GFP. What’s more, we might have the ability to form differernt SPOTs in the cell and regulate them respectively. The functions of SPOT can also diverse. We can build a real time sensor for molecule in living cells to monitoring the concentration changing in environment or in cells. More metabolism pathway can be test in SPOT and we will find some laws of the function of regulate the metabolism. To be summary, more achievement is coming true with SPOT.</p>
 
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Latest revision as of 10:39, 17 October 2018

Public Engagement

Public Engagement

Talking to high school students

One of our team members, Guo Fuyu, went to Hutian Middle School in Huaihua, Hunan Province. He introduced systems and synthetic biology to the students and helped them with biology in high school as well. In our view, it is of fundamental significance to provide as much middle school students in second-tier cities in China as possible with access to frontier science, since quality education is definitely as important as examination-oriented education. (Figure. 1)


Guo Fuyu talking with middle school students in Hutian Middle School

According to a survey in Peking University, freshmen who have had a sense of higher education and sought for their interest in high school, get accustomed to college life and study remarkably faster than those who haven’t. We genuinely hope university students and professors across China can communicate more with high school students and help every single one find his or her interest worth pursuing their whole life as soon as possible. We especially hope that the students in second-tier cities and rural areas get the same chance of quality education as those in megacities.

Pre-school scientific education

While the idea to introduce the most cutting-edge science to children in kindergarten may sound outlandish, we can still spend a nice day with them and introduce them to science. Two of our team members did this in the kindergarten attached to Peking University. We designed a series of games with a science background: demonstrating the three phases of water, observing phase separation, constructing a “phase separation” system with magnetic balls, and water drawing. The kids liked these games very much which inspired us a lot.

It is a big challenge for us to tell the children about basic science, but we’re happy to see them enjoying the games which is also interesting and relaxing for us. We enjoyed the fascination with science, which can cross the boundary of age and life experience.

Kids playing marbling paint together Ouyang Xiaoyi teaching kids about three states of water

This activity made us confident about the perspective of broad-based scientific communication, and we realized the we can communicate in both a “meaningful” and “interesting” way, where all the participants are equal and relaxed and the conversation is much more efficient.

Documentation of Peking iGEM as enlightenment for beginners

We have built up a WeChat public platform which is a worldwide platform with billions of users for documentation, communication and popularization. To give the future igemers a taste of igem projects and help them learn the basic rudiments of synthetic biology we have reviewed the projects of Peking iGEM in the past 14 years. All these articles are rather approachable and most of them received positive feedbacks. We demonstrate here the articles and hope it may help more people who want to get to know about synthetic biology.

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Popular video about phase separation in biology

We made a popular video about phase separation in biology to introduce it to more people studying the subject. We posted it on several websites in China and many undergraduates and graduates were introduced to phase separation through our video. We also found it necessary to communicate more about basic knowledge of different disciplines in the area of systems biology. This is arguably the best way in which people can learn about the system they work on and cooperate better with each other.
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