Difference between revisions of "Team:CPU CHINA/Experiments"

"<h2>Sample preparation</h2>"+

"<h2>Sample preparation</h2>"+

"<h4>determine the volume of protein extract to ensure 50 μg in each well.</h4>"+

"<h4>determine the volume of protein extract to ensure 50 μg in each well.</h4>"+

"<h4>2.Add 5 μL sample buffer to the sample, and make the volume in each lane equalized using double distilled H2O (dd H2O). Mix well. (Tip: Total volume of 15 μL per lane is suggested).</h4>"+

"<h4>2.Add 5 μL sample buffer to the sample, and make the volume in each lane equalized using double distilled H2O (dd H2O). Mix well. (Tip: Total volume of 15 μL per lane is suggested).</h4>"+

"<h2>Gel preparation</h2>"+

"<h2>Gel preparation</h2>"+

"<h4>1.After preparing the 10% stacking gel solution, assemble the rack for gel solidification. (Tip: 10% AP and TEMED solidify the solution; therefore, both gels can be prepared at the same time, if the abovementioned reagents are not added until the end).</h4>"+

"<h4>1.After preparing the 10% stacking gel solution, assemble the rack for gel solidification. (Tip: 10% AP and TEMED solidify the solution; therefore, both gels can be prepared at the same time, if the abovementioned reagents are not added until the end).</h4>"+

"<h4>2.Add stacking gel solution carefully until the level is equal to the green bar holding the glass plates. Add H2O to the top. Wait for 15–30 minutes until the gel turning solidified. (Tip: Using a suction pipette can make the process of adding the gel to the glass plate easier).</h4>"+

"<h4>2.Add stacking gel solution carefully until the level is equal to the green bar holding the glass plates. Add H2O to the top. Wait for 15–30 minutes until the gel turning solidified. (Tip: Using a suction pipette can make the process of adding the gel to the glass plate easier).</h4>"+