Difference between revisions of "Team:CPU CHINA/Experiments"

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         <center><h4 style="font-size:unset !important">You can click on these arrows to read the details of our experiments.</h4></center>
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         <center><h4 style="font-size:unset !important">You can click on these arrows to read the details of our experiments.</h4><br></center>
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        <center><h4 style="font-size:unset !important">You can <a href="javascript:void(0);" onclick="showExperiment(5)">click here to see our Promoter</a>.</h4></center>
 
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"<h4>4.Add distilled H2O to 1L</h4>"+
 
"<h4>4.Add distilled H2O to 1L</h4>"+
 
"<h4>5.Sterilize by filtration or autoclaving</h4><br>";
 
"<h4>5.Sterilize by filtration or autoclaving</h4><br>";
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var qidongzi = "<h2>Cell transfection and luciferase reporter gene assay</h2>"+
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"<h4>pGL3-Basic was used as negative Control, and pGL3-Control was used as positive Control.</h2>"+
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"<h4>HepG2 cells were transfected with 1ug of pGL3-htert, pGL3-hulc and pGL3-control. HepG2 cells were cultured in DEME medium containing 10% heat inactivated bovine serum at 37 ℃ and 5% CO2 constant temperature incubator. HepG2 cells were seeded in 96-well plate according to 2* 10<sup>5</sup>/ well, and cultured in non-antibiotic culture medium for 24 hours, then transfected with liposome (Lipo fectamin3000), and transfected with 3 holes per plasmid. The transfection medium was replaced after 4 to 6 h, and the DEME culture medium containing 10% calf serum was used to continue the culture. After 48 h, the cells were treated with Promege's <a><u>steady-glo Luciferase Assay System kit </u></a>and the activity of <i>Luc</i> was determined.</h4><br>";
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document.getElementById("showdivTitle").innerHTML="Western Blot";
 
document.getElementById("showdivTitle").innerHTML="Western Blot";
  
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document.getElementById("showdivContent-txt").innerHTML=qidongzi;
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document.getElementById("showdivTitle").innerHTML="Promoter";
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Revision as of 13:19, 17 October 2018

You can click on these arrows to read the details of our experiments.


You can click here to see our Promoter.