Difference between revisions of "Team:Tongji China/Protocol"

Line 110: Line 110:
 
                                 <li><a data-scroll="" href="#3">Hela Cell</a>
 
                                 <li><a data-scroll="" href="#3">Hela Cell</a>
 
                                 </li>
 
                                 </li>
                                 <li><a data-scroll="" href="#4">Mouse<a name="1">&emsp;</a></a>
+
                                 <li><a data-scroll="" href="#4">Mouse</a>
 
                                 </li>
 
                                 </li>
 
                                  
 
                                  
Line 119: Line 119:
  
  
 +
<br><br>
 
<font size = "2">Please wait for the loading, if you want to read these hidden stuff. </font>
 
<font size = "2">Please wait for the loading, if you want to read these hidden stuff. </font>
 
<br><br>
 
<br><br>
Line 169: Line 170:
  
 
<details>
 
<details>
<summary>Restriction Digestion<a name="2">&emsp;</a></summary>
+
<summary>Restriction Digestion</summary>
 
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/2d/T--Tongji_China--protocol-1-8.pdf" ><br>
 
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/2d/T--Tongji_China--protocol-1-8.pdf" ><br>
 
<a href="https://static.igem.org/mediawiki/2018/2/2d/T--Tongji_China--protocol-1-8.pdf" download="">Download</a>
 
<a href="https://static.igem.org/mediawiki/2018/2/2d/T--Tongji_China--protocol-1-8.pdf" download="">Download</a>
Line 176: Line 177:
  
  
<br><br>
+
<br><a name="2">.</a><br><br><br>
 
<h1>Pseudomonas aeruginosa</h1><br>
 
<h1>Pseudomonas aeruginosa</h1><br>
 
<details>
 
<details>
Line 197: Line 198:
  
 
<details>
 
<details>
<summary>Western Blot<a name="3">&emsp;</a></summary>
+
<summary>Western Blot</summary>
 
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/8/8e/T--Tongji_China--protocol-2-4.pdf" ><br>
 
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/8/8e/T--Tongji_China--protocol-2-4.pdf" ><br>
 
<a href="https://static.igem.org/mediawiki/2018/8/8e/T--Tongji_China--protocol-2-4.pdf" download="">Download</a>
 
<a href="https://static.igem.org/mediawiki/2018/8/8e/T--Tongji_China--protocol-2-4.pdf" download="">Download</a>
 
</details>
 
</details>
  
<br><br>
+
<br><a name="3">.</a><br><br><br>
  
  
Line 220: Line 221:
  
 
<details>
 
<details>
<summary>Thawing Frozen Cells<a name="4">&emsp;</a></summary>
+
<summary>Thawing Frozen Cells</summary>
 
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/f/fe/T--Tongji_China--protocol-3-3.pdf" ><br>
 
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/f/fe/T--Tongji_China--protocol-3-3.pdf" ><br>
 
<a href="https://static.igem.org/mediawiki/2018/f/fe/T--Tongji_China--protocol-3-3.pdf" download="">Download</a>
 
<a href="https://static.igem.org/mediawiki/2018/f/fe/T--Tongji_China--protocol-3-3.pdf" download="">Download</a>
Line 226: Line 227:
  
  
<br><br>
+
<br><a name="4">.</a><br><br><br>
  
 
<h1>Mouse</h1><br>
 
<h1>Mouse</h1><br>

Revision as of 14:00, 17 October 2018

Protocol
Wet Lab
Protocol



Please wait for the loading, if you want to read these hidden stuff.

Plasmid

Annealing Oilgos
Download
Chemical Transformation
Download
Colnony PCR
Download
Consumables
Download
DNA Gel Electrophoresis
Download
Gel Extraction & Minipreps
Download
Ligation
Download
Restriction Digestion
Download

.


Pseudomonas aeruginosa


Colony PCR of Pseudomonas aeruginosa
Download
Creating Bacterial Glycerol Stocks
Download
Electroporation
Download
Western Blot
Download

.


Hela Cell


Immunofluorescence Staining
Download
Passage HeLa Cells
Download
Thawing Frozen Cells
Download

.


Mouse


Oral Gavage of Mice
Download
Immunohistochemistry
Download