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<h2>Background</h2> | <h2>Background</h2> | ||
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<h3>Biosensors</h3> | <h3>Biosensors</h3> | ||
− | <p>Biosensors are devices that are able to detect the presence of analytes and effectively | + | <p style="width:1200px;font-size:18px;">Biosensors are devices that are able to detect the presence of analytes and effectively |
convert this biological response to an electrical signal. This system consists of three components: a bioreceptor, transducer and detector. The | convert this biological response to an electrical signal. This system consists of three components: a bioreceptor, transducer and detector. The | ||
bioreceptor is able to form substrate-specific interactions with the analyte. The transducer is then able to detect the substrate-receptor | bioreceptor is able to form substrate-specific interactions with the analyte. The transducer is then able to detect the substrate-receptor | ||
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<h3>Ligand Binding Domain</h3> | <h3>Ligand Binding Domain</h3> | ||
− | <p>Nuclear receptors are a family of evolutionarily conserved proteins that functions as a | + | <p style="width:1200px;font-size:18px;">Nuclear receptors are a family of evolutionarily conserved proteins that functions as a |
ligand-dependent transcription factor [1]. After binding certain ligands, the receptor undergoes a conformational change which activates them, | ligand-dependent transcription factor [1]. After binding certain ligands, the receptor undergoes a conformational change which activates them, | ||
and allows them to bind directly to DNA to alter gene transcription [1]. Circulating steroid hormones, like cortisol, are able to activate the | and allows them to bind directly to DNA to alter gene transcription [1]. Circulating steroid hormones, like cortisol, are able to activate the | ||
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in the nuclear receptor ligand binding domain to produce a measurable signal.</figcaption> | in the nuclear receptor ligand binding domain to produce a measurable signal.</figcaption> | ||
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<h3>Approaches</h3> | <h3>Approaches</h3> | ||
− | <p>Starting with the natural Ligand Binding Domain of nuclear receptors as our means of binding to ligands, we took two approaches to producing a | + | <p style="width:1200px;font-size:18px;">Starting with the natural Ligand Binding Domain of nuclear receptors as our means of binding to ligands, we took two approaches to producing a |
measurable signal from this interaction. | measurable signal from this interaction. | ||
− | <br><a href="https://2018.igem.org/Team:Queens_Canada/Design"><em>Please click here to see our approaches and design process</em></a></p> | + | <br><a href="https://2018.igem.org/Team:Queens_Canada/Design"><em>Please click here to see our approaches and design process.</em></a></p> |
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+ | <h5>References</h5> | ||
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+ | T. Kino, Glucocorticoid Receptor. MDText.com, Inc., 2000.</li> | ||
+ | <li>H. Reyer, S. Ponsuksili, E. Kanitz, R. Pöhland, K. Wimmers, and E. Murani, “A Natural Mutation in Helix 5 of the Ligand Binding Domain of Glucocorticoid Receptor Enhances Receptor-Ligand Interaction,” PLoS One, vol. 11, no. 10, p. e0164628, Oct. 2016.</li> | ||
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<footer style="background-color: #212121;height:90px;"> | <footer style="background-color: #212121;height:90px;"> | ||
<div class="container"> | <div class="container"> |
Latest revision as of 15:15, 17 October 2018
Background
Biosensors
Biosensors are devices that are able to detect the presence of analytes and effectively convert this biological response to an electrical signal. This system consists of three components: a bioreceptor, transducer and detector. The bioreceptor is able to form substrate-specific interactions with the analyte. The transducer is then able to detect the substrate-receptor interaction and transmit this into an electrical signal, which is amplified and processed by the detector. This information is then capable of being sent to a data storage device for quantification and analytical purposes.
Ligand Binding Domain
Nuclear receptors are a family of evolutionarily conserved proteins that functions as a ligand-dependent transcription factor [1]. After binding certain ligands, the receptor undergoes a conformational change which activates them, and allows them to bind directly to DNA to alter gene transcription [1]. Circulating steroid hormones, like cortisol, are able to activate the receptor and mediate processes such as stress response, energy metabolism and immune responses [2]. The ligand binding domain of nuclear receptors generally consists of eleven alpha-helices and two beta-sheets that enable the formation of a three-layered protein structure [2]. There also exists a regulatory C-terminal helix, titled "helix 12”, that is essential for hormone binding. There are conserved residues within these helices which form critical interactions with the ligand allowing for specificity within the interaction [2].
Approaches
Starting with the natural Ligand Binding Domain of nuclear receptors as our means of binding to ligands, we took two approaches to producing a
measurable signal from this interaction.
Please click here to see our approaches and design process.
References
- T. Kino, Glucocorticoid Receptor. MDText.com, Inc., 2000.
- H. Reyer, S. Ponsuksili, E. Kanitz, R. Pöhland, K. Wimmers, and E. Murani, “A Natural Mutation in Helix 5 of the Ligand Binding Domain of Glucocorticoid Receptor Enhances Receptor-Ligand Interaction,” PLoS One, vol. 11, no. 10, p. e0164628, Oct. 2016.