Difference between revisions of "Team:Tongji China/Protocol"
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<title>Protocol</title> | <title>Protocol</title> | ||
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</head> | </head> | ||
| + | <body> | ||
| + | <div class = "background"> | ||
| + | <div class="content"> | ||
| + | <div class="title"> | ||
| + | Wet Lab | ||
| + | </div> | ||
| + | <div class="logoPicture"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/d/d8/T--Tongji_China--picture-wetlab-protocol-0.png" height="25%" width=15%" /> | ||
| + | </div> | ||
| + | <div class="title_2"> | ||
| + | Protocol | ||
| + | </div><br> | ||
| + | |||
| + | <div id="navWrap" class=""> | ||
| + | <div id="subNav" style="text-align:center"> | ||
| + | <center><ul> | ||
| + | <li><a data-scroll="" href="#1">Plasmid</a> | ||
| + | </li> | ||
| + | <li><a data-scroll="" href="#2"><em><I>Pseudomonas aeruginosa</I></em></a> | ||
| + | </li> | ||
| + | <li><a data-scroll="" href="#3">HeLa Cell</a> | ||
| + | </li> | ||
| + | <li><a data-scroll="" href="#4">Mouse<a name="1"> </a></a> | ||
| + | </li> | ||
| + | |||
| + | </ul></center> | ||
| + | <br class="clearLeft"> | ||
| + | </div> | ||
| + | </div> | ||
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| + | |||
| + | <font size = "2">Please wait for the loading, if you want to read these hidden stuff. </font> | ||
| + | <br><br> | ||
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| + | <h1>Plasmid</h1> | ||
| + | |||
| + | <div class="multi-summary"> | ||
| + | <details> | ||
| + | <summary>Annealing Oilgos</summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/c/c5/T--Tongji_China--protocol-1-1.pdf" width="100%" internalinstanceid="6"><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/c/c5/T--Tongji_China--protocol-1-1.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | |||
| + | <details> | ||
| + | <summary>Chemical Transformation</summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/b7/T--Tongji_China--protocol-1-2.pdf" internalinstanceid="10" /><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/b/b7/T--Tongji_China--protocol-1-2.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | |||
| + | <details> | ||
| + | <summary>Colony PCR</summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/bc/T--Tongji_China--protocol-1-3.pdf" internalinstanceid="14"><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/b/bc/T--Tongji_China--protocol-1-3.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | |||
| + | <details> | ||
| + | <summary>Consumables</summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/25/T--Tongji_China--protocol-1-4.pdf" internalinstanceid="14"><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/2/25/T--Tongji_China--protocol-1-4.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | |||
| + | <details> | ||
| + | <summary>DNA Gel Electrophoresis</summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/1/10/T--Tongji_China--protocol-1-5.pdf" ><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/1/10/T--Tongji_China--protocol-1-5.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | |||
| + | <details> | ||
| + | <summary>Gel Extraction & Minipreps</summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/3/37/T--Tongji_China--protocol-1-6.pdf" ><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/3/37/T--Tongji_China--protocol-1-6.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | |||
| + | <details> | ||
| + | <summary>Ligation</summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/e/e3/T--Tongji_China--protocol-1-7.pdf" ><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/e/e3/T--Tongji_China--protocol-1-7.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | |||
| + | <details> | ||
| + | <summary>Restriction Digestion<a name="2"> </a></summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/2d/T--Tongji_China--protocol-1-8.pdf" ><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/2/2d/T--Tongji_China--protocol-1-8.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | |||
| + | |||
| + | |||
| + | <br><br> | ||
| + | <h1><I>Pseudomonas aeruginosa</I></h1><br> | ||
| + | <details> | ||
| + | <summary>Colony PCR of Pseudomonas aeruginosa</summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/5/50/T--Tongji_China--protocol-2-1.pdf" ><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/5/50/T--Tongji_China--protocol-2-1.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | |||
| + | <details> | ||
| + | <summary>Creating Bacterial Glycerol Stocks</summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-2-2.pdf" ><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-2-2.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | |||
| + | <details> | ||
| + | <summary>Electroporation</summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/2f/T--Tongji_China--protocol-2-3.pdf" ><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/2/2f/T--Tongji_China--protocol-2-3.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | |||
| + | <details> | ||
| + | <summary>Western Blot<a name="3"> </a></summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/8/8e/T--Tongji_China--protocol-2-4.pdf" ><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/8/8e/T--Tongji_China--protocol-2-4.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | |||
| + | <br><br> | ||
| + | |||
| + | |||
| + | |||
| + | <h1>HeLa Cell</h1><br> | ||
| + | <details> | ||
| + | <summary>Immunofluorescence Staining</summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/6/62/T--Tongji_China--protocol-3-1.pdf" ><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/6/62/T--Tongji_China--protocol-3-1.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | |||
| + | <details> | ||
| + | <summary>Passage HeLa Cells</summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-3-2.pdf" ><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-3-2.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | |||
| + | <details> | ||
| + | <summary>Thawing Frozen Cells<a name="4"> </a></summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/f/fe/T--Tongji_China--protocol-3-3.pdf" ><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/f/fe/T--Tongji_China--protocol-3-3.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | |||
| + | |||
| + | <br><br> | ||
| + | <h1>Mouse</h1><br> | ||
| + | <details> | ||
| + | <summary>Oral Gavage of Mice</summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/b4/T--Tongji_China--protocol-4-1.pdf" ><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/b/b4/T--Tongji_China--protocol-4-1.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | <details> | ||
| + | <summary>Immunohistochemistry</summary> | ||
| + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/4/43/T--Tongji_China--protocol-4-2.pdf" ><br> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/4/43/T--Tongji_China--protocol-4-2.pdf" download="">Download</a> | ||
| + | </details> | ||
| + | </div> | ||
| + | <br> | ||
| + | </div></div> | ||
| + | </body> | ||
</html> | </html> | ||
{{Tongji_China/Footer_N}} | {{Tongji_China/Footer_N}} | ||
Latest revision as of 18:17, 17 October 2018
Wet Lab
Protocol
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