Difference between revisions of "Team:Tongji China/Protocol"

 
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<title>Protocol</title>
 
<title>Protocol</title>
 
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                         <div id="subNav" style="text-align:center">
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                             <ul>
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                             <center><ul>
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                                 <li><a data-scroll="" href="#gluconacetobacter"><em>G. xylinus</em></a>
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                                 <li><a data-scroll="" href="#1">Plasmid</a>
 
                                 </li>
 
                                 </li>
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                                 <li><a data-scroll="" href="#general">General/<em>E. coli</em></a>
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                                 <li><a data-scroll="" href="#2"><em><I>Pseudomonas aeruginosa</I></em></a>
 
                                 </li>
 
                                 </li>
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                                 <li><a data-scroll="" href="#functionalisation">Functionalisation</a>
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                                 <li><a data-scroll="" href="#3">HeLa Cell</a>
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                                </li>
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                                <li><a data-scroll="" href="#4">Mouse<a name="1">&emsp;</a></a>
 
                                 </li>
 
                                 </li>
 
                                  
 
                                  
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                             </ul>
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                             </ul></center>
 
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                         </div>
 
                         </div>
 
                     </div>
 
                     </div>
  
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<section class="first">
 
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<h1>General Protocols</h1>
 
  
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<div class="multi-summary protocols">
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<font size = "2">Please wait for the loading, if you want to read these hidden stuff. </font>
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<br><br>
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<h1>Plasmid</h1>
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<div class="multi-summary">
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<details>
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<summary>Annealing Oilgos</summary>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/c/c5/T--Tongji_China--protocol-1-1.pdf" width="100%" internalinstanceid="6"><br>
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<a href="https://static.igem.org/mediawiki/2018/c/c5/T--Tongji_China--protocol-1-1.pdf" download="">Download</a>
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</details>
 +
 
 
<details>
 
<details>
 
<summary>Chemical Transformation</summary>
 
<summary>Chemical Transformation</summary>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" width="800" internalinstanceid="6">
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/b7/T--Tongji_China--protocol-1-2.pdf" internalinstanceid="10" /><br>
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<a href="https://static.igem.org/mediawiki/2017/a/ad/T--ETH_Zurich--chemicaltransformation.pdf" download="">Download</a>
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<a href="https://static.igem.org/mediawiki/2018/b/b7/T--Tongji_China--protocol-1-2.pdf" download="">Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
 
<summary>Colony PCR</summary>
 
<summary>Colony PCR</summary>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" width="800" internalinstanceid="10">
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/bc/T--Tongji_China--protocol-1-3.pdf" internalinstanceid="14"><br>
−
<a href="https://static.igem.org/mediawiki/2017/5/57/T--ETH_Zurich--colonypcr.pdf" download="">Download</a>
+
<a href="https://static.igem.org/mediawiki/2018/b/bc/T--Tongji_China--protocol-1-3.pdf" download="">Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
 
<summary>Consumables</summary>
 
<summary>Consumables</summary>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/e/e1/T--ETH_Zurich--consumables.pdf" width="800" internalinstanceid="14">
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/25/T--Tongji_China--protocol-1-4.pdf" internalinstanceid="14"><br>
−
<a href="https://static.igem.org/mediawiki/2017/e/e1/T--ETH_Zurich--consumables.pdf" download="">Download</a>
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<a href="https://static.igem.org/mediawiki/2018/2/25/T--Tongji_China--protocol-1-4.pdf" download="">Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
<summary>Cryostock of Bacteria</summary>
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<summary>DNA Gel Electrophoresis</summary>
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--cryostock.pdf" width="800">
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/1/10/T--Tongji_China--protocol-1-5.pdf" ><br>
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<a href="https://static.igem.org/mediawiki/2017/2/2f/T--ETH_Zurich--cryostock.pdf" download="">Download</a>
+
<a href="https://static.igem.org/mediawiki/2018/1/10/T--Tongji_China--protocol-1-5.pdf" download="">Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
<summary>Electroporation</summary>
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<summary>Gel Extraction & Minipreps</summary>
−
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" width="800">
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/3/37/T--Tongji_China--protocol-1-6.pdf" ><br>
−
<a href="https://static.igem.org/mediawiki/2017/6/6c/T--ETH_Zurich--electroporation.pdf" download="">Download</a>
+
<a href="https://static.igem.org/mediawiki/2018/3/37/T--Tongji_China--protocol-1-6.pdf" download="">Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
<summary>Gel Extraction</summary>
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<summary>Ligation</summary>
−
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/4/4b/T--ETH_Zurich--gelextraction.pdf" width="800">
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/e/e3/T--Tongji_China--protocol-1-7.pdf" ><br>
−
<a href="https://static.igem.org/mediawiki/2017/4/4b/T--ETH_Zurich--gelextraction.pdf" download="">Download</a>
+
<a href="https://static.igem.org/mediawiki/2018/e/e3/T--Tongji_China--protocol-1-7.pdf" download="">Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
<summary>Gibson Assembly</summary>
+
<summary>Restriction Digestion<a name="2">&emsp;</a></summary>
−
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/d/dc/T--ETH_Zurich--gibsonassembly.pdf" width="800">
+
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/2d/T--Tongji_China--protocol-1-8.pdf" ><br>
−
<a href="https://static.igem.org/mediawiki/2017/d/dc/T--ETH_Zurich--gibsonassembly.pdf" download="">Download</a>
+
<a href="https://static.igem.org/mediawiki/2018/2/2d/T--Tongji_China--protocol-1-8.pdf" download="">Download</a>
 
</details>
 
</details>
  
 +
 +
 +
<br><br>
 +
<h1><I>Pseudomonas aeruginosa</I></h1><br>
 
<details>
 
<details>
−
<summary>Ligase Chain Reaction</summary>
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<summary>Colony PCR of Pseudomonas aeruginosa</summary>
−
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/1/1c/T--ETH_Zurich--LCR.pdf" width="800">
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/5/50/T--Tongji_China--protocol-2-1.pdf" ><br>
−
<a href="https://static.igem.org/mediawiki/2017/1/1c/T--ETH_Zurich--LCR.pdf" download="">Download</a>
+
<a href="https://static.igem.org/mediawiki/2018/5/50/T--Tongji_China--protocol-2-1.pdf" download="">Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
<summary>Miniprep</summary>
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<summary>Creating Bacterial Glycerol Stocks</summary>
−
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--miniprep.pdf" width="800">
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-2-2.pdf" ><br>
−
<a href="https://static.igem.org/mediawiki/2017/0/02/T--ETH_Zurich--miniprep.pdf" download="">Download</a>
+
<a href="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-2-2.pdf" download="">Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
<summary>PCR Clean-Up</summary>
+
<summary>Electroporation</summary>
−
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PCRcleanup.pdf" width="800">
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/2f/T--Tongji_China--protocol-2-3.pdf" ><br>
−
<a href="https://static.igem.org/mediawiki/2017/2/20/T--ETH_Zurich--PCRcleanup.pdf" download="">Download</a>
+
<a href="https://static.igem.org/mediawiki/2018/2/2f/T--Tongji_China--protocol-2-3.pdf" download="">Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
<summary>Phusion PCR</summary>
+
<summary>Western Blot<a name="3">&emsp;</a></summary>
−
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/0/08/T--ETH_Zurich--Phusionpcr.pdf" width="800">
+
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/8/8e/T--Tongji_China--protocol-2-4.pdf" ><br>
−
<a href="https://static.igem.org/mediawiki/2017/0/08/T--ETH_Zurich--Phusionpcr.pdf" download="">Download</a>
+
<a href="https://static.igem.org/mediawiki/2018/8/8e/T--Tongji_China--protocol-2-4.pdf" download="">Download</a>
 
</details>
 
</details>
  
 +
<br><br>
 +
 +
 +
 +
<h1>HeLa Cell</h1><br>
 
<details>
 
<details>
−
<summary>Preparation of Chemically Competent Cells</summary>
+
<summary>Immunofluorescence Staining</summary>
−
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/b/b5/T--ETH_Zurich--chemicallycompetent.pdf" width="800">
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/6/62/T--Tongji_China--protocol-3-1.pdf" ><br>
−
<a href="https://static.igem.org/mediawiki/2017/b/b5/T--ETH_Zurich--chemicallycompetent.pdf" download="">Download</a>
+
<a href="https://static.igem.org/mediawiki/2018/6/62/T--Tongji_China--protocol-3-1.pdf" download="">Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
<summary>Preparation of Electrocompetent Cells</summary>
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<summary>Passage HeLa Cells</summary>
−
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/e/e3/T--ETH_Zurich--electrocompetent.pdf" width="800">
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-3-2.pdf" ><br>
−
<a href="https://static.igem.org/mediawiki/2017/e/e3/T--ETH_Zurich--electrocompetent.pdf" download="">Download</a>
+
<a href="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-3-2.pdf" download="">Download</a>
 
</details>
 
</details>
  
 
<details>
 
<details>
−
<summary>Restriction Ligation</summary>
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<summary>Thawing Frozen Cells<a name="4">&emsp;</a></summary>
−
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" width="800">
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<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/f/fe/T--Tongji_China--protocol-3-3.pdf" ><br>
−
<a href="https://static.igem.org/mediawiki/2017/5/56/T--ETH_Zurich--restrictionligation.pdf" download="">Download</a>
+
<a href="https://static.igem.org/mediawiki/2018/f/fe/T--Tongji_China--protocol-3-3.pdf" download="">Download</a>
 
</details>
 
</details>
−
</div>
 
−
</section>
 
  
  
 +
<br><br>
  
 +
<h1>Mouse</h1><br>
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<details>
 +
<summary>Oral Gavage of Mice</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/b4/T--Tongji_China--protocol-4-1.pdf" ><br>
 +
<a href="https://static.igem.org/mediawiki/2018/b/b4/T--Tongji_China--protocol-4-1.pdf" download="">Download</a>
 +
</details>
 +
 +
<details>
 +
<summary>Immunohistochemistry</summary>
 +
<embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/4/43/T--Tongji_China--protocol-4-2.pdf" ><br>
 +
<a href="https://static.igem.org/mediawiki/2018/4/43/T--Tongji_China--protocol-4-2.pdf" download="">Download</a>
 +
</details>
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 +
</div>
 
<br>
 
<br>
 
         </div></div>
 
         </div></div>

Latest revision as of 18:17, 17 October 2018

Protocol
Wet Lab
Protocol

Please wait for the loading, if you want to read these hidden stuff.

Plasmid

Annealing Oilgos
Download
Chemical Transformation
Download
Colony PCR
Download
Consumables
Download
DNA Gel Electrophoresis
Download
Gel Extraction & Minipreps
Download
Ligation
Download
Restriction Digestion 
Download


Pseudomonas aeruginosa


Colony PCR of Pseudomonas aeruginosa
Download
Creating Bacterial Glycerol Stocks
Download
Electroporation
Download
Western Blot 
Download


HeLa Cell


Immunofluorescence Staining
Download
Passage HeLa Cells
Download
Thawing Frozen Cells 
Download


Mouse


Oral Gavage of Mice
Download
Immunohistochemistry
Download

Contact

College of Life Science and Technology

Tongji university

No.1239, Yangpu District, Shanghai, China

Email: tongjiigem2018@126.com