Difference between revisions of "Team:Tongji China/Protocol"
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<title>Protocol</title> | <title>Protocol</title> | ||
<style> | <style> | ||
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| + | height: 600px; | ||
| + | } | ||
| + | h1{ | ||
| + | width: 100%; | ||
| + | color: #BC818D; | ||
| + | margin-left: 0%; | ||
| + | height: auto; | ||
| + | font-size: 1.5em; | ||
| + | font-family: Trebuchet MS; | ||
| + | font-weight: 550; | ||
| + | } | ||
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.multi-summary { | .multi-summary { | ||
display: flex; | display: flex; | ||
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.multi-summary > details:first-child { | .multi-summary > details:first-child { | ||
| − | margin-top: | + | margin-top: 5%; |
} | } | ||
.multi-summary > details:last-child { | .multi-summary > details:last-child { | ||
| − | margin-bottom: | + | margin-bottom: 5%; |
} | } | ||
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background: #44474B; | background: #44474B; | ||
width: 100%; | width: 100%; | ||
| − | margin-bottom: | + | margin-bottom: 3%; |
} | } | ||
#subNav { | #subNav { | ||
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<div id="navWrap" class=""> | <div id="navWrap" class=""> | ||
| − | <div id="subNav"> | + | <div id="subNav" style="text-align:center"> |
| − | <ul> | + | <center><ul> |
| − | <li><a data-scroll="" href="# | + | <li><a data-scroll="" href="#1">Plasmid</a> |
</li> | </li> | ||
| − | <li><a data-scroll="" href="# | + | <li><a data-scroll="" href="#2"><em><I>Pseudomonas aeruginosa</I></em></a> |
</li> | </li> | ||
| − | <li><a data-scroll="" href="# | + | <li><a data-scroll="" href="#3">HeLa Cell</a> |
| + | </li> | ||
| + | <li><a data-scroll="" href="#4">Mouse<a name="1"> </a></a> | ||
</li> | </li> | ||
| − | </ul> | + | </ul></center> |
<br class="clearLeft"> | <br class="clearLeft"> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | < | + | |
| + | <font size = "2">Please wait for the loading, if you want to read these hidden stuff. </font> | ||
| + | <br><br> | ||
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<h1>Plasmid</h1> | <h1>Plasmid</h1> | ||
| − | <div class="multi-summary | + | <div class="multi-summary"> |
<details> | <details> | ||
<summary>Annealing Oilgos</summary> | <summary>Annealing Oilgos</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/c/c5/T--Tongji_China--protocol-1-1.pdf" width=" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/c/c5/T--Tongji_China--protocol-1-1.pdf" width="100%" internalinstanceid="6"><br> |
<a href="https://static.igem.org/mediawiki/2018/c/c5/T--Tongji_China--protocol-1-1.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/c/c5/T--Tongji_China--protocol-1-1.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<details> | <details> | ||
<summary>Chemical Transformation</summary> | <summary>Chemical Transformation</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/b7/T--Tongji_China--protocol-1-2.pdf" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/b7/T--Tongji_China--protocol-1-2.pdf" internalinstanceid="10" /><br> |
<a href="https://static.igem.org/mediawiki/2018/b/b7/T--Tongji_China--protocol-1-2.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/b/b7/T--Tongji_China--protocol-1-2.pdf" download="">Download</a> | ||
</details> | </details> | ||
<details> | <details> | ||
| − | <summary> | + | <summary>Colony PCR</summary> |
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/bc/T--Tongji_China--protocol-1-3.pdf" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/bc/T--Tongji_China--protocol-1-3.pdf" internalinstanceid="14"><br> |
<a href="https://static.igem.org/mediawiki/2018/b/bc/T--Tongji_China--protocol-1-3.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/b/bc/T--Tongji_China--protocol-1-3.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<details> | <details> | ||
<summary>Consumables</summary> | <summary>Consumables</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/25/T--Tongji_China--protocol-1-4.pdf" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/25/T--Tongji_China--protocol-1-4.pdf" internalinstanceid="14"><br> |
<a href="https://static.igem.org/mediawiki/2018/2/25/T--Tongji_China--protocol-1-4.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/2/25/T--Tongji_China--protocol-1-4.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<details> | <details> | ||
<summary>DNA Gel Electrophoresis</summary> | <summary>DNA Gel Electrophoresis</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/1/10/T--Tongji_China--protocol-1-5.pdf" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/1/10/T--Tongji_China--protocol-1-5.pdf" ><br> |
<a href="https://static.igem.org/mediawiki/2018/1/10/T--Tongji_China--protocol-1-5.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/1/10/T--Tongji_China--protocol-1-5.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<details> | <details> | ||
<summary>Gel Extraction & Minipreps</summary> | <summary>Gel Extraction & Minipreps</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/3/37/T--Tongji_China--protocol-1-6.pdf" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/3/37/T--Tongji_China--protocol-1-6.pdf" ><br> |
<a href="https://static.igem.org/mediawiki/2018/3/37/T--Tongji_China--protocol-1-6.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/3/37/T--Tongji_China--protocol-1-6.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<details> | <details> | ||
<summary>Ligation</summary> | <summary>Ligation</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/e/e3/T--Tongji_China--protocol-1-7.pdf" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/e/e3/T--Tongji_China--protocol-1-7.pdf" ><br> |
<a href="https://static.igem.org/mediawiki/2018/e/e3/T--Tongji_China--protocol-1-7.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/e/e3/T--Tongji_China--protocol-1-7.pdf" download="">Download</a> | ||
</details> | </details> | ||
<details> | <details> | ||
| − | <summary>Restriction Digestion</summary> | + | <summary>Restriction Digestion<a name="2"> </a></summary> |
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/2d/T--Tongji_China--protocol-1-8.pdf" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/2d/T--Tongji_China--protocol-1-8.pdf" ><br> |
<a href="https://static.igem.org/mediawiki/2018/2/2d/T--Tongji_China--protocol-1-8.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/2/2d/T--Tongji_China--protocol-1-8.pdf" download="">Download</a> | ||
</details> | </details> | ||
| − | <h1>Pseudomonas aeruginosa</h1> | + | |
| + | |||
| + | <br><br> | ||
| + | <h1><I>Pseudomonas aeruginosa</I></h1><br> | ||
<details> | <details> | ||
<summary>Colony PCR of Pseudomonas aeruginosa</summary> | <summary>Colony PCR of Pseudomonas aeruginosa</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/5/50/T--Tongji_China--protocol-2-1.pdf" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/5/50/T--Tongji_China--protocol-2-1.pdf" ><br> |
<a href="https://static.igem.org/mediawiki/2018/5/50/T--Tongji_China--protocol-2-1.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/5/50/T--Tongji_China--protocol-2-1.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<details> | <details> | ||
<summary>Creating Bacterial Glycerol Stocks</summary> | <summary>Creating Bacterial Glycerol Stocks</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-2-2.pdf" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-2-2.pdf" ><br> |
<a href="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-2-2.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-2-2.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<details> | <details> | ||
<summary>Electroporation</summary> | <summary>Electroporation</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/2f/T--Tongji_China--protocol-2-3.pdf" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/2/2f/T--Tongji_China--protocol-2-3.pdf" ><br> |
<a href="https://static.igem.org/mediawiki/2018/2/2f/T--Tongji_China--protocol-2-3.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/2/2f/T--Tongji_China--protocol-2-3.pdf" download="">Download</a> | ||
</details> | </details> | ||
<details> | <details> | ||
| − | <summary>Western Blot</summary> | + | <summary>Western Blot<a name="3"> </a></summary> |
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/8/8e/T--Tongji_China--protocol-2-4.pdf" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/8/8e/T--Tongji_China--protocol-2-4.pdf" ><br> |
<a href="https://static.igem.org/mediawiki/2018/8/8e/T--Tongji_China--protocol-2-4.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/8/8e/T--Tongji_China--protocol-2-4.pdf" download="">Download</a> | ||
</details> | </details> | ||
| − | <h1> | + | <br><br> |
| + | |||
| + | |||
| + | |||
| + | <h1>HeLa Cell</h1><br> | ||
<details> | <details> | ||
<summary>Immunofluorescence Staining</summary> | <summary>Immunofluorescence Staining</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/6/62/T--Tongji_China--protocol-3-1.pdf" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/6/62/T--Tongji_China--protocol-3-1.pdf" ><br> |
<a href="https://static.igem.org/mediawiki/2018/6/62/T--Tongji_China--protocol-3-1.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/6/62/T--Tongji_China--protocol-3-1.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<details> | <details> | ||
<summary>Passage HeLa Cells</summary> | <summary>Passage HeLa Cells</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-3-2.pdf" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-3-2.pdf" ><br> |
<a href="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-3-2.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/a/af/T--Tongji_China--protocol-3-2.pdf" download="">Download</a> | ||
</details> | </details> | ||
<details> | <details> | ||
| − | <summary>Thawing Frozen Cells</summary> | + | <summary>Thawing Frozen Cells<a name="4"> </a></summary> |
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/f/fe/T--Tongji_China--protocol-3-3.pdf" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/f/fe/T--Tongji_China--protocol-3-3.pdf" ><br> |
<a href="https://static.igem.org/mediawiki/2018/f/fe/T--Tongji_China--protocol-3-3.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/f/fe/T--Tongji_China--protocol-3-3.pdf" download="">Download</a> | ||
</details> | </details> | ||
| − | <h1>Mouse</h1> | + | |
| + | <br><br> | ||
| + | |||
| + | <h1>Mouse</h1><br> | ||
<details> | <details> | ||
<summary>Oral Gavage of Mice</summary> | <summary>Oral Gavage of Mice</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/b4/T--Tongji_China--protocol-4-1.pdf" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/b/b4/T--Tongji_China--protocol-4-1.pdf" ><br> |
<a href="https://static.igem.org/mediawiki/2018/b/b4/T--Tongji_China--protocol-4-1.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/b/b4/T--Tongji_China--protocol-4-1.pdf" download="">Download</a> | ||
</details> | </details> | ||
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<details> | <details> | ||
<summary>Immunohistochemistry</summary> | <summary>Immunohistochemistry</summary> | ||
| − | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/4/43/T--Tongji_China--protocol-4-2.pdf" | + | <embed type="application/pdf" src="https://static.igem.org/mediawiki/2018/4/43/T--Tongji_China--protocol-4-2.pdf" ><br> |
<a href="https://static.igem.org/mediawiki/2018/4/43/T--Tongji_China--protocol-4-2.pdf" download="">Download</a> | <a href="https://static.igem.org/mediawiki/2018/4/43/T--Tongji_China--protocol-4-2.pdf" download="">Download</a> | ||
</details> | </details> | ||
| − | </ | + | </div> |
<br> | <br> | ||
</div></div> | </div></div> | ||
Latest revision as of 18:17, 17 October 2018
Wet Lab
Protocol
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