Difference between revisions of "Team:HUST-China/Composite part"
Zyz2018hust (Talk | contribs) |
|||
| Line 252: | Line 252: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533000</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533000" style="color:blue;"><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533000" style="color:blue;">BBa_K2533000</td> |
<td>RBS-ldhD-RBS-lldP</td> | <td>RBS-ldhD-RBS-lldP</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533001</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533001" style="color:blue;">BBa_K2533001</a></td> |
<td>RBS-ldhDC-RBS-lldP</td> | <td>RBS-ldhDC-RBS-lldP</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533002</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533002" style="color:blue;">BBa_K2533002</a></td> |
<td>RBS-ldhDnARSdR-lldP</td> | <td>RBS-ldhDnARSdR-lldP</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533003</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533003" style="color:blue;">BBa_K2533003</a></td> |
<td>RBS-ldhDARSdR-lldP</td> | <td>RBS-ldhDARSdR-lldP</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533014</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533014" style="color:blue;">BBa_K2533014</a></td> |
<td>RBS-TH-RBS-gldA-RBS-LLDP</td> | <td>RBS-TH-RBS-gldA-RBS-LLDP</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533016</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533000" style="color:blue;">BBa_K2533016</a></td> |
<td>RBS-ldhD-FLAG-RBS-lldP-his tag</td> | <td>RBS-ldhD-FLAG-RBS-lldP-his tag</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533017</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533017" style="color:blue;">BBa_K2533017</a></td> |
<td>RBS-ldhDC-FLAG-RBS-lldP-his tag</td> | <td>RBS-ldhDC-FLAG-RBS-lldP-his tag</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533018</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533018" style="color:blue;">BBa_K2533018</a></td> |
<td>RBS-ldhDnARSdR-FLAG-RBS-lldP-his tag</td> | <td>RBS-ldhDnARSdR-FLAG-RBS-lldP-his tag</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533019</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533019" style="color:blue;">BBa_K2533019</a></td> |
<td>RBS-ldhDARSdR-FLAG-RBS-lldP-his tag</td> | <td>RBS-ldhDARSdR-FLAG-RBS-lldP-his tag</td> | ||
| Line 310: | Line 310: | ||
<div class="col-md-12"> | <div class="col-md-12"> | ||
| − | <p> | + | <p>BBa_K2533016, BBa_K2533017, BBa_K2533018, BBa_K2533019</p> |
| − | BBa_K2533016, BBa_K2533017, BBa_K2533018, BBa_K2533019</p> | + | |
</div> | </div> | ||
<div class="col-md-6 col-md-offset-2" > <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/87/T--HUST-China--2018-composite_part-picture2.png.png"></div> | <div class="col-md-6 col-md-offset-2" > <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/87/T--HUST-China--2018-composite_part-picture2.png.png"></div> | ||
| + | </div> | ||
<div class="container"> | <div class="container"> | ||
| Line 320: | Line 320: | ||
</div> | </div> | ||
| − | + | ||
| + | <div class="row"> | ||
| + | <div class="col-md-12 info-blocks"> | ||
| + | <i class="icon-info-blocks material-icons"><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/6/63/T--HUST-China--2018-zhaozehong01.png"></i> | ||
| + | <div class="info-blocks-in" style="background-color: #ffffff; border: 1px solid #eeeeee;border-radius:5px;"> | ||
<h3>1.2. Rhodopseudomonas palustris</h3> | <h3>1.2. Rhodopseudomonas palustris</h3> | ||
| − | |||
<div class="col-md-10 col-md-offset-1 table-responsive"> | <div class="col-md-10 col-md-offset-1 table-responsive"> | ||
<table class="table table-bordered table-hover" style="text-align: center;"> | <table class="table table-bordered table-hover" style="text-align: center;"> | ||
| Line 331: | Line 334: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533045</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533045" style="color:blue;">BBa_K2533045</a></td> |
<td>RBS-mleS</td> | <td>RBS-mleS</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533046</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533046" style="color:blue;">BBa_K2533046</a></td> |
<td>RBS-lldP</td> | <td>RBS-lldP</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533047</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533047" style="color:blue;">BBa_K2533047</a></td> |
<td>RBS-ldhA</td> | <td>RBS-ldhA</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533048</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533000" style="color:blue;">BBa_K2533048</a></td> |
<td>RBS-mleS-RBS-lldP</td> | <td>RBS-mleS-RBS-lldP</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533049</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533049" style="color:blue;">BBa_K2533049</a></td> |
<td>RBS-lldP-RBS-ldhA</td> | <td>RBS-lldP-RBS-ldhA</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533050</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533050" style="color:blue;">BBa_K2533050</a></td> |
<td>mleS-lldp-ldhA</td> | <td>mleS-lldp-ldhA</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533051</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533051" style="color:blue;">BBa_K2533051</td> |
<td>mleS-lldP-TT</td> | <td>mleS-lldP-TT</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533052</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533052" style="color:blue;">BBa_K2533052</a></td> |
<td>RBS-lldP-RBS-ldhA-TT</td> | <td>RBS-lldP-RBS-ldhA-TT</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533053</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533053" style="color:blue;">BBa_K2533053</a></td> |
<td>RBS-mleS-RBS-lldP-RBS-ldhA-TT</td> | <td>RBS-mleS-RBS-lldP-RBS-ldhA-TT</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533054</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533054" style="color:blue;">BBa_K2533054</a></td> |
<td>RBS-ldhA-RBS-lldP</td> | <td>RBS-ldhA-RBS-lldP</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533055</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533055" style="color:blue;">BBa_K2533055</a></td> |
<td>RBS-ldhA-RBS-lldP-TT</td> | <td>RBS-ldhA-RBS-lldP-TT</td> | ||
| Line 404: | Line 407: | ||
</div> | </div> | ||
</div> | </div> | ||
| − | + | </div> | |
<div class="row"> | <div class="row"> | ||
<div class="col-md-12 content-text"> | <div class="col-md-12 content-text"> | ||
<div class="about-logo"> | <div class="about-logo"> | ||
| − | |||
| − | |||
| − | |||
<h3><strong>Part2: <span class="red-content">Electrogenic microorganism system: chemical energy→electric energy</span></strong></h3> | <h3><strong>Part2: <span class="red-content">Electrogenic microorganism system: chemical energy→electric energy</span></strong></h3> | ||
</div> | </div> | ||
| Line 417: | Line 417: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-md-12 info-blocks"> | <div class="col-md-12 info-blocks"> | ||
| − | <i class="icon-info-blocks material-icons"><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/ | + | <i class="icon-info-blocks material-icons"><img class="img-responsive" src="https://static.igem.org/mediawiki/2018/0/0c/T--HUST-China--2018-xiwa01.png"></i> |
<div class="info-blocks-in" style="background-color: #ffffff; border: 1px solid #eeeeee;border-radius:5px;"> | <div class="info-blocks-in" style="background-color: #ffffff; border: 1px solid #eeeeee;border-radius:5px;"> | ||
<h3>2.1. Shewanella(S.oneidensis)</h3> | <h3>2.1. Shewanella(S.oneidensis)</h3> | ||
| Line 428: | Line 428: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533030</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533030" style="color:blue;">BBa_K2533030</a></td> |
<td>RBS-dld</td> | <td>RBS-dld</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533031</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533031" style="color:blue;">BBa_K2533031</a></td> |
<td>RBS-lldEFG</td> | <td>RBS-lldEFG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533032</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533032" style="color:blue;">BBa_K2533032</a></td> |
<td>RBS-dld-TT-Ptac-RBS-lldEFG</td> | <td>RBS-dld-TT-Ptac-RBS-lldEFG</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533033</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533033" style="color:blue;">BBa_K2533033</a></td> |
<td>RBS-gapA</td> | <td>RBS-gapA</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533034</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533034" style="color:blue;">BBa_K2533034</a></td> |
<td>RBS-mdh</td> | <td>RBS-mdh</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533035</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533035" style="color:blue;">BBa_K2533035</a></td> |
<td>RBS-gapA-RBS-mdh-TT</td> | <td>RBS-gapA-RBS-mdh-TT</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533036</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533036" style="color:blue;">BBa_K2533036</td> |
<td>RBS-pflB</td> | <td>RBS-pflB</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533037</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533037" style="color:blue;">BBa_K2533037</a></td> |
<td>RBS-fdh</td> | <td>RBS-fdh</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533038</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533038" style="color:blue;">BBa_K2533038</a></td> |
<td>RBS-pflB-RBS-fdh</td> | <td>RBS-pflB-RBS-fdh</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533039</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533039" style="color:blue;">BBa_K2533039</a></td> |
<td>RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh</td> | <td>RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533056</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533056" style="color:blue;">BBa_K2533056</a></td> |
<td>PlacIq-lacI-Ptac-RBS-gapA</td> | <td>PlacIq-lacI-Ptac-RBS-gapA</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533057</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533057" style="color:blue;">BBa_K2533057</a></td> |
<td>PlacIq-lacI-Ptac-RBS-mdh</td> | <td>PlacIq-lacI-Ptac-RBS-mdh</td> | ||
Revision as of 19:12, 17 October 2018
Composite part
Part1: Photosynthetic microorganism system: optical energy→chemical energy
1.1 Synechocystis sp. PCC 6803(cyanobacteria)
| Name | Description |
|---|---|
| BBa_K2533000 | RBS-ldhD-RBS-lldP |
| BBa_K2533001 | RBS-ldhDC-RBS-lldP |
| BBa_K2533002 | RBS-ldhDnARSdR-lldP |
| BBa_K2533003 | RBS-ldhDARSdR-lldP |
| BBa_K2533014 | RBS-TH-RBS-gldA-RBS-LLDP |
| BBa_K2533016 | RBS-ldhD-FLAG-RBS-lldP-his tag |
| BBa_K2533017 | RBS-ldhDC-FLAG-RBS-lldP-his tag |
| BBa_K2533018 | RBS-ldhDnARSdR-FLAG-RBS-lldP-his tag |
| BBa_K2533019 | RBS-ldhDARSdR-FLAG-RBS-lldP-his tag |
BBa_K2533000, BBa_K2533001, BBa_K2533002, BBa_K2533003, BBa_K2533014
BBa_K2533016, BBa_K2533017, BBa_K2533018, BBa_K2533019
1.2. Rhodopseudomonas palustris
| Name | Description |
|---|---|
| BBa_K2533045 | RBS-mleS |
| BBa_K2533046 | RBS-lldP |
| BBa_K2533047 | RBS-ldhA |
| BBa_K2533048 | RBS-mleS-RBS-lldP |
| BBa_K2533049 | RBS-lldP-RBS-ldhA |
| BBa_K2533050 | mleS-lldp-ldhA |
| BBa_K2533051 | mleS-lldP-TT |
| BBa_K2533052 | RBS-lldP-RBS-ldhA-TT |
| BBa_K2533053 | RBS-mleS-RBS-lldP-RBS-ldhA-TT |
| BBa_K2533054 | RBS-ldhA-RBS-lldP |
| BBa_K2533055 | RBS-ldhA-RBS-lldP-TT |
BBa_K2533050, BBa_K2533048, BBa_K2533054, BBa_K2533049
Our photosynthetic microorganism system is consist of Synechocystis sp. PCC 6803(cyanobacteria) and Rhodopseudomonas palustris. They are all modified to produce lactate to improve the electrogenic efficiency of Shewanella(S.oneidensis).
Part2: Electrogenic microorganism system: chemical energy→electric energy
2.1. Shewanella(S.oneidensis)
| Name | Description |
|---|---|
| BBa_K2533030 | RBS-dld |
| BBa_K2533031 | RBS-lldEFG |
| BBa_K2533032 | RBS-dld-TT-Ptac-RBS-lldEFG |
| BBa_K2533033 | RBS-gapA |
| BBa_K2533034 | RBS-mdh |
| BBa_K2533035 | RBS-gapA-RBS-mdh-TT |
| BBa_K2533036 | RBS-pflB |
| BBa_K2533037 | RBS-fdh |
| BBa_K2533038 | RBS-pflB-RBS-fdh |
| BBa_K2533039 | RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh |
| BBa_K2533056 | PlacIq-lacI-Ptac-RBS-gapA |
| BBa_K2533057 | PlacIq-lacI-Ptac-RBS-mdh |
BBa_K2533030, BBa_K2533031, BBa_K2533032, BBa_K2533033, BBa_K2533034, BBa_K2533036, BBa_K2533037
BBa_K2533035, BBa_K2533038, BBa_K2533039
Our MFC produces electricity by Shewanella(S.oneidensis) through transfer chemical energy into electric energy. As a result, we design these parts to strengthen the ability of Shewanella(S.oneidensis) to absorb lactate，with an intention to boost the efficiency of electrogenic microorganism system.
