Difference between revisions of "Team:BIT-China/Demonstrate"

 
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    <script src="https://2018.igem.org/Template:BIT-China/js/base-loading?action=raw&ctype=text/javascript"></script>
 
  
 
</head>
 
</head>
  
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<body id="ibody" class="scoll_dis">
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<body>
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          <ul id="left-nav">
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    <ul id="left-nav">
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         <li>
 
         <li>
 
             <a>PROJECT</a>
 
             <a>PROJECT</a>
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                 <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsFeedback">Feedback</a></li>
 
                 <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsFeedback">Feedback</a></li>
 
                 <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsOutput">Output</a></li>
 
                 <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsOutput">Output</a></li>
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                 <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsInput">Input</a></li>
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                 <li><a href="https://2018.igem.org/Team:BIT-China/Results">Results</a></li>
 
             </ul>
 
             </ul>
 
         </li>
 
         </li>
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             <ul>
 
             <ul>
 
                 <li><a href="https://2018.igem.org/Team:BIT-China/Model">Overview</a></li>
 
                 <li><a href="https://2018.igem.org/Team:BIT-China/Model">Overview</a></li>
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                 <li><a href="https://2018.igem.org/Team:BIT-China/FluorescentProbesModel">Fluorescent Probes Model </a></li>
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                 <li><a href="https://2018.igem.org/Team:BIT-China/FluorescentProbesModel">Fluorescent Probe Model </a></li>
 
                 <li><a href="https://2018.igem.org/Team:BIT-China/H2O2DecompositionModel">H<sub>2</sub>O<sub>2</sub>
 
                 <li><a href="https://2018.igem.org/Team:BIT-China/H2O2DecompositionModel">H<sub>2</sub>O<sub>2</sub>
 
                         Decomposition Model</a></li>
 
                         Decomposition Model</a></li>
  
 
                 <li><a href="https://2018.igem.org/Team:BIT-China/roGFP2-Orp1MichaelisEquationModel">roGFP2-Orp1
 
                 <li><a href="https://2018.igem.org/Team:BIT-China/roGFP2-Orp1MichaelisEquationModel">roGFP2-Orp1
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                         Michaelis equations Model</a></li>
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                         Michaelis equation Model</a></li>
 
             </ul>
 
             </ul>
 
         </li>
 
         </li>
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         </li>
 
         </li>
 
     </ul>
 
     </ul>
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     <a href="https://2018.igem.org/Team:BIT-China"><img id="imgA" class="imgA-new-pos" src="https://static.igem.org/mediawiki/2018/4/46/T--BIT-China--iGEM2018-A_img.png" /></a>
 
     <a href="https://2018.igem.org/Team:BIT-China"><img id="imgA" class="imgA-new-pos" src="https://static.igem.org/mediawiki/2018/4/46/T--BIT-China--iGEM2018-A_img.png" /></a>
 
     <!-- end -->
 
     <!-- end -->
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     <img style="position:fixed;height:100vh;top:0;right:0;" src="https://static.igem.org/mediawiki/2018/0/09/T--BIT-China--iGEM2018-achievement-bg.png">
 
     <img style="position:fixed;height:100vh;top:0;right:0;" src="https://static.igem.org/mediawiki/2018/0/09/T--BIT-China--iGEM2018-achievement-bg.png">
  
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     <div id="parts-content-all" class="content_container" style="margin-top: calc(25vh - 30px);">
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     <div id="parts-content-all" class="content_container" style="margin-top: calc(25vh - 30px);width: 50vw;">
 
         <div class="cd-section">
 
         <div class="cd-section">
 
             <div class="PT-title-1">
 
             <div class="PT-title-1">
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             <div class="PT-content-all">
 
             <div class="PT-content-all">
 
                 <div class="PT-content">
 
                 <div class="PT-content">
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                     <p class="PT-content-p">
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                     <figure class="EXP-Fig">
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                         Our engineered yeast should finally have the function of monitoring antioxidants in real time.
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                         <a href="https://2018.igem.org/Team:BIT-China/Parts" target="_Blank"> <img src="https://static.igem.org/mediawiki/2018/a/a1/T--BIT-China--Demonstrate1Parts.png"></a>
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                        To demonstrate this, we integrated the two subsystems together into ∆<i>yca1</i> yeast and had
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                     </figure>
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                        series of tests on it, in which the increasing of ROS in yeast can be effectively captured by
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                     <figure class="EXP-Fig">
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                        our roGFP2-Orp1 protein.
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                         <a href="https://2018.igem.org/Team:BIT-China/Design" target="_Blank"> <img src="https://static.igem.org/mediawiki/2018/c/c5/T--BIT-China--Demonstrate2GeneCircuit.png"></a>
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                     </p>
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                    </figure>
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                    <figure class="EXP-Fig">
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                     <p class="PT-content-p">
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                         <a href="https://2018.igem.org/Team:BIT-China/Results" target="_Blank"> <img src="https://static.igem.org/mediawiki/2018/d/d1/T--BIT-China--Demonstrate3Results.png"></a>
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                         In order to regulate endogenous ROS, we replaced yeast endogenous YNO1 <b>promotor</b> with a
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                     </figure>
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                        galactose-inducible promotor, GAL1-GAL10. We then constructed the plasmid
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                     <figure class="EXP-Fig">
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                        pESC-TEF1p-roGFP2-Orp1-CYC1t, a shuttle vector marked with <i>trp1</i>. Cellular redox status
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                         <a href="https://2018.igem.org/Team:BIT-China/HPOverview" target="_Blank"> <img src="https://static.igem.org/mediawiki/2018/4/45/T--BIT-China--Demonstrate4HP.png"></a>
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                        were monitored by measuring the fluorescence ratio at 488 nm(reduced state) and 405 nm(oxidized
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                     </figure>
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                         state). Cells were cultured in SD medium supplemented with 1% galactose at 30 ℃, during which
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                     <figure class="EXP-Fig">
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                        GAL1-GAL10 promotor was induced when galactose added. It suggests that overexpression of <i>yno1</i>yno1
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                         <a href="https://2018.igem.org/Team:BIT-China/Collaborations" target="_Blank"> <img src="https://static.igem.org/mediawiki/2018/f/fa/T--BIT-China--Demonstrate5Collaborations.png"></a>
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                        gene will increase cells ROS level and decline the fluorescence ratio at 488 nm and 405 nm,
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                    </figure>
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                        which means there are more ROS in our modified strain.
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                    <figure class="EXP-Fig">
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                     </p>
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                         <a href="https://2018.igem.org/Team:BIT-China/Model" target="_Blank"> <img src="https://static.igem.org/mediawiki/2018/c/c1/T--BIT-China--Demonstrate6Modeling.png"></a>
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                     </figure>
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                     <p class="PT-content-p">
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                     <figure class="EXP-Fig">
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                         As the results showing, our output subsystem can reflect the accumulation of endogenous ROS
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                         <a href="https://2018.igem.org/Team:BIT-China/Attributions" target="_Blank"> <img src="https://static.igem.org/mediawiki/2018/6/6f/T--BIT-China--Demonstrate7Attributions.png"></a>
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                        caused by regulator subsystem(figure). A simple decription.
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                     </p>
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                     <p class="PT-content-p">
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                         Since we have proved the feasibility that the antioxidative ability of antioxidant can be
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                        measured by fluorescence probes(6.3.4). We can easily set up the relationship between our
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                        system and real antioxidative ability of antioxidant. However, due to time limitation, the
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                        final results will only be shown during the Giant Jamboree. Our presentation will on Saturday
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                         3:15 PM - 3:45 PM, Room 312 and our poster will at Zone 4 - #235 and welcome to us to see our
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                        great work!
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                     </p>
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                     <figure class="MD-Fig">
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                         <img src="">
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                        <figcaption>缺图</figcaption>
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                     </figure>
 
                     </figure>
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                 </div>
 
                 </div>
 
             </div>
 
             </div>
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             <p style="font-size:15px;margin: 0;padding: 0;margin-top: 6px;color:  rgb(161, 161, 161);">
 
             <p style="font-size:15px;margin: 0;padding: 0;margin-top: 6px;color:  rgb(161, 161, 161);">
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                 Copyright © 2018 BIT-China
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                 Copyright © 2018 BIT-China
 
             </p>
 
             </p>
 
         </div>
 
         </div>

Latest revision as of 19:55, 17 October 2018

DEMONSTRATE

Contact

Institute of Biotransformation and synthetic biosystem School of Chemistry and Chemical Engineering.

Beijing Institute of Technology

100081, Beijing

Email:lichun@bit.edu.cn

Copyright © 2018 BIT-China