Difference between revisions of "Team:BIT-China/Parts"

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{{BIT-China}}
 
 
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<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h3>Note</h3>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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    <link rel="stylesheet" href="https://2018.igem.org/Template:BIT-China/css/common-style?action=raw&amp;ctype=text/css">
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<h3>Adding parts to the registry</h3>
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        .table-body table tr:nth-child(2n+1) {
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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        .table-head td,
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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ADD PARTS
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</a>
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<h3>Inspiration</h3>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<body>
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<ul>
+
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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</ul>
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</div>
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</div>
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 +
          <ul id="left-nav">
 +
        <li>
 +
            <a>PROJECT</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Background">Background</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Description">Description</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Design">Idea & Design</a></li>
 +
            </ul>
 +
        </li>
  
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<div class="clear extra_space"></div>
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        <li>
 +
            <a>EXPERIMENTS</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsRegulator">Regulator</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsFeedback">Feedback</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsOutput">Output</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Results">Results</a></li>
 +
            </ul>
 +
        </li>
  
 +
        <li>
 +
            <a>MODELING</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Model">Overview</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/FluorescentProbesModel">Fluorescent Probe Model </a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/H2O2DecompositionModel">H<sub>2</sub>O<sub>2</sub>
 +
                        Decomposition Model</a></li>
  
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/roGFP2-Orp1MichaelisEquationModel">roGFP2-Orp1
 +
                        Michaelis equation Model</a></li>
 +
            </ul>
 +
        </li>
  
 +
        <li>
 +
            <a>HUMAN PRACTICES</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/HPOverview">Overview</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Human_Practices">Integrated Human Practices</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Public_Engagement">Education & Public Engagement</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Collaborations">Collaborations</a></li>
 +
            </ul>
 +
        </li>
  
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<div class="column full_size">
+
        <li>
 +
            <a>NOTEBOOK</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Notebook">Lab Book</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Protocols">Methodology / Protocols</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Equipment">Material & Equipment</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/InterLab">Measurement / InterLab</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Safety">Safety</a></li>
 +
            </ul>
 +
        </li>
  
−
<h3>What information do I need to start putting my parts on the Registry?</h3>
+
        <li>
−
<p>The information needed to initially create a part on the Registry is:</p>
+
            <a>ACHIEVEMENTS</a>
−
<ul>
+
            <ul>
−
<li>Part Name</li>
+
                <li><a href="https://2018.igem.org/Team:BIT-China/JudgingForm">Judging Form</a></li>
−
<li>Part type</li>
+
                <li><a href="https://2018.igem.org/Team:BIT-China/Parts">Parts</a></li>
−
<li>Creator</li>
+
                <li><a href="https://2018.igem.org/Team:BIT-China/Improve">Improve</a></li>
−
<li>Sequence</li>
+
                <li><a href="https://2018.igem.org/Team:BIT-China/Applied_Design">Applied Design</a></li>
−
<li>Short Description (60 characters on what the DNA does)</li>
+
                <li><a href="https://2018.igem.org/Team:BIT-China/Demonstrate">Demonstrate</a></li>
−
<li>Long Description (Longer description of what the DNA does)</li>
+
            </ul>
−
<li>Design considerations</li>
+
        </li>
−
</ul>
+
  
−
<p>
+
        <li>
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
+
            <a>TEAM</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Team">Members</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Attributions">Attributions</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Gallery">Gallery</a></li>
 +
            </ul>
 +
        </li>
 +
    </ul>
  
−
</div>
+
    <a href="https://2018.igem.org/Team:BIT-China"><img id="imgA" class="imgA-new-pos" src="https://static.igem.org/mediawiki/2018/4/46/T--BIT-China--iGEM2018-A_img.png" /></a>
 +
    <!-- end -->
  
 +
    <div id="parts-content-all" class="content_container" style="margin-top: calc(25vh - 30px);">
 +
        <div class="cd-section">
 +
            <div class="PT-title-1">
 +
                <a style="border-bottom: 3px solid #131313;text-decoration: none;color: #131313;">PARTS</a>
 +
            </div>
 +
        </div>
  
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                <div class="PT-content">
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                    <p class="PT-content-p">
 +
                        In 2018, BIT-China has submitted 16 parts. We divide our parts into 3 group, Regulator,
 +
                        Feedback and Output. Each group contains some parts with similar function or close relation.
 +
                        Different groups also connect with each other.
 +
                    </p>
 +
                </div>
 +
            </div>
 +
        </div>
  
−
<div class="column full_size">
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        <div id="PT1" class="cd-section">
−
<h3>Part Table </h3>
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            <div class="PT-title-2 PT-margin-Title2Up">
 +
                <a style="text-decoration: none;color: #131313;">Regulator</a>
 +
            </div>
  
−
<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
+
            <div class="PT-content-all">
 +
                <div class="PT-title-3 PT-margin-toTitle2">
 +
                    <a style="text-decoration: none;color: #131313;">Increase ROS Content </a>
 +
                </div>
  
−
</html>
+
                <div class="PT-content">
−
<groupparts>iGEM18 BIT-China</groupparts>
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                    <p class="PT-content-p PT-margin-toTitle3">
−
<html>
+
                        BBa_K2765026 and BBa_K2765021 is a collection of <b>regulate components</b> including coding
−
</div>
+
                        protein and expression cassette, which can increase the accumulation of intracellular ROS
 +
                        through overexpressing yon1 induced by galactose.
 +
                    </p>
 +
                </div>
 +
 
 +
                <div class="PT-title-3 PT-margin-Title3Up">
 +
                    <a style="text-decoration: none;color: #131313;">Improve Tolerance</a>
 +
                </div>
 +
 
 +
                <div class="PT-content">
 +
                    <p class="PT-content-p PT-margin-toTitle3">
 +
                        BBa_K2765030 is 1000bp homologous arm of <i>yca1 </i>gene, which can knock out <i>yca1</i> gene
 +
                        through
 +
                        homologous recombination so that the yeast's tolerance to H<sub>2</sub>O<sub>2</sub> could be
 +
                        improved.
 +
                    </p>
 +
                </div>
 +
            </div>
 +
        </div>
 +
 
 +
        <div id="PT2" class="cd-section">
 +
            <div class="PT-title-2 PT-margin-Title2Up">
 +
                <a style="text-decoration: none;color: #131313;">Feedback</a>
 +
            </div>
 +
 
 +
            <div class="PT-content-all">
 +
                <div class="PT-title-3 PT-margin-Title3Up">
 +
                    <a style="text-decoration: none;color: #131313;">Control ROS Content</a>
 +
                </div>
 +
                <div class="PT-content">
 +
                    <p class="PT-content-p PT-margin-toTitle3">
 +
                        BBa_K2765000~BBa_K2765005 is a collection of <b>feedback components</b> that are downstream
 +
                        gene
 +
                        promoter of Yap1. We use them to turn on the expression of dCas9 which can inhibit the
 +
                        expression of yno1 gene. Considering the application of our system in the future, to realize
 +
                        different feedback effect, we verified different promoters, GLR1p, SOD2p, TRR1p, TRX2p and GSH1p,
 +
                        with different transcription level.
 +
                    </p>
 +
                </div>
 +
            </div>
 +
        </div>
 +
 
 +
        <div id="PT3" class="cd-section">
 +
            <div class="PT-title-2 PT-margin-Title2Up">
 +
                <a style="text-decoration: none;color: #131313;">Output</a>
 +
            </div>
 +
            <div class="PT-content-all">
 +
                <div class="PT-title-3 PT-margin-Title3Up">
 +
                    <a style="text-decoration: none;color: #131313;">Detect ROS content</a>
 +
                </div>
 +
 
 +
                <div class="PT-content">
 +
                    <p class="PT-content-p PT-margin-toTitle3">
 +
                        BBa_K2765040~BBa_K2765053 is a collection of <b>output components</b> including promoters,
 +
                        coding
 +
                        protein and expression cassette, which can express redox-sensitive green fluorescent protein
 +
                        roGFP2-Orp1 and display ROS content in cells by its fluorescence ratio.
 +
                    </p>
 +
 
 +
                    <p class="PT-content-p PT-margin-ContentPUp">
 +
                        According to this measurement principle, the concentration of whole roGFP2-Orp1 can influence
 +
                        the final signal output strength, even detect the same sample, so we verified four promoters,
 +
                        FBA1, TEF2, TEF1 and ENO2, with different transcription level to solve this interfere.
 +
                        Considering the application of our system in the future, we made this collection and wished
 +
                        these parts can be used in different antioxidant detection.
 +
                    </p>
 +
                </div>
 +
 
 +
                <div class="PT-title-3 PT-margin-Title3Up">
 +
                    <a style="text-decoration: none;color: #131313;">Improve a previous part</a>
 +
                </div>
 +
 
 +
                <div class="PT-content">
 +
                    <p class="PT-content-p PT-margin-toTitle3">
 +
                        We performed codon optimization on team Connell 2017's previous part <a style="color:#131313;" href="http://parts.igem.org/Part:BBa_K2296006"
 +
                            target="_Blank">BBa_K2296006</a> and obtained our core part BBa_K2296006, which codes for roGFP2-Orp1 protein. Also we got As a result, it is more suitable
 +
                        to our chassis organisms---yeast and has better expression in yeast. <a style="text-decoration: none;color:#131313;"
 +
                            href="https://2018.igem.org/Team:BIT-China/Improve">Check
 +
                            here to know further
 +
                            information.</a>
 +
                    </p>
 +
                </div>
 +
            </div>
 +
        </div>
 +
 
 +
        <div id="PT4" class="cd-section" style="margin-top: 20px;text-align: center;font-family: 'helveticaregular';color: #131313;">
 +
            <div class="PT-title-2 PT-margin-Title2Up">
 +
                <a style="text-decoration: none;color: #131313;">Part Form</a>
 +
            </div>
 +
 
 +
            <div class="PT-content-all PT-margin-toTitle2">
 +
                <div class="PT-content">
 +
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 +
                        <table>
 +
                            <colgroup>
 +
                                <col style="width:50px;" />
 +
                                <col style="width: 150px;" />
 +
                                <col style="width: 200px;" />
 +
                                <col style="width: 200px;" />
 +
                                <col />
 +
                            </colgroup>
 +
                            <thead>
 +
                                <tr style="font-size: 20px;">
 +
                                    <th>No.</th>
 +
                                    <th>Biobrick</th>
 +
                                    <th>Name</th>
 +
                                    <th>Description</th>
 +
                                    <th>Length</th>
 +
                                </tr>
 +
                            </thead>
 +
                        </table>
 +
                    </div>
 +
                    <div class="table-body">
 +
                        <table>
 +
                            <colgroup>
 +
                                <col style="width: 50px;" />
 +
                                <col style="width: 150px;" />
 +
                                <col style="width: 200px;" />
 +
                                <col style="width: 200px;" />
 +
                                <col />
 +
                            </colgroup>
 +
                            <tbody style="font-size: 16px;">
 +
                                <tr>
 +
                                    <td colspan="5" style="font-family:'kg_second_chances_solidRg';">Regulator</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>1</td>
 +
                                    <td>
 +
                                        <a href="http://parts.igem.org/Part:BBa_K2765021" target="_Blank">BBa_K2765021</a>
 +
                                    </td>
 +
                                    <td>yno1</td>
 +
                                    <td>ROS Content Increaser</td>
 +
                                    <td>1713 bp</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>2</td>
 +
                                    <td>
 +
                                        <a href="http://parts.igem.org/Part:BBa_K2765026" target="_Blank">BBa_K2765026</a>
 +
                                    </td>
 +
                                    <td>GAL1p-yno1-CYC1t</td>
 +
                                    <td>Increase ROS Content </td>
 +
                                    <td>2651 bp</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>3</td>
 +
                                    <td>
 +
                                        <a href="http://parts.igem.org/Part:BBa_K2765030" target="_Blank">BBa_K2765030</a>
 +
                                    </td>
 +
                                    <td>homologous arm-ura(△yca1)</td>
 +
                                    <td>Knock out the yca1 gene</td>
 +
                                    <td>3038 bp</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td colspan="5" style="font-family:'kg_second_chances_solidRg';">Feedback</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>4</td>
 +
                                    <td>
 +
                                        <a href="http://parts.igem.org/Part:BBa_K2765000" target="_Blank">BBa_K2765000</a>
 +
                                    </td>
 +
                                    <td>GAL1p</td>
 +
                                    <td>Turn on the expression of dCas9</td>
 +
                                    <td>332 bp</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>5</td>
 +
                                    <td>
 +
                                        <a href="http://parts.igem.org/Part:BBa_K2765001" target="_Blank">BBa_K2765001</a>
 +
                                    </td>
 +
                                    <td>SOD2p</td>
 +
                                    <td>Turn on the expression of dCas9</td>
 +
                                    <td>526 bp</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>6</td>
 +
                                    <td>
 +
                                        <a href="http://parts.igem.org/Part:BBa_K2765002" target="_Blank">BBa_K2765002</a>
 +
                                    </td>
 +
                                    <td>TRR1p</td>
 +
                                    <td>Turn on the expression of dCas9</td>
 +
                                    <td>960 bp</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>7</td>
 +
                                    <td>
 +
                                        <a href="http://parts.igem.org/Part:BBa_K2765003" target="_Blank">BBa_K2765003</a>
 +
                                    </td>
 +
                                    <td>TRX2p</td>
 +
                                    <td>Turn on the expression of dCas9</td>
 +
                                    <td>283 bp</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>8</td>
 +
                                    <td>
 +
                                        <a href="http://parts.igem.org/Part:BBa_K2765005" target="_Blank">BBa_K2765005</a>
 +
                                    </td>
 +
                                    <td>GSH1p</td>
 +
                                    <td>Turn on the expression of dCas9</td>
 +
                                    <td>2027 bp</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td colspan="5" style="font-family:'kg_second_chances_solidRg';">Output</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>9</td>
 +
                                    <td>
 +
                                        <a href="http://parts.igem.org/Part:BBa_K2765040" target="_Blank">BBa_K2765040</a>
 +
                                    </td>
 +
                                    <td>FBA1p</td>
 +
                                    <td>Turn on the expression of roGFP2-Orp1</td>
 +
                                    <td>631 bp</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>10</td>
 +
                                    <td>
 +
                                        <a href="http://parts.igem.org/Part:BBa_K2765042" target="_Blank">BBa_K2765042</a>
 +
                                    </td>
 +
                                    <td>ENO2p</td>
 +
                                    <td>Turn on the expression of roGFP2-Orp1</td>
 +
                                    <td>512 bp</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>11</td>
 +
                                    <td>
 +
                                        <a href="http://parts.igem.org/Part:BBa_K2765043" target="_Blank">BBa_K2765043</a>
 +
                                    </td>
 +
                                    <td>roGFP2-Orp1</td>
 +
                                    <td>ROS Content Detector</td>
 +
                                    <td>1317 bp</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>12</td>
 +
                                    <td>
 +
                                        <a href="http://parts.igem.org/Part:BBa_K2765045" target="_Blank">BBa_K2765045</a>
 +
                                    </td>
 +
                                    <td>TEF1p</td>
 +
                                    <td>Turn on the expression of roGFP2-Orp1</td>
 +
                                    <td>402 bp</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>13</td>
 +
                                    <td>
 +
                                        <a href="http://parts.igem.org/Part:BBa_K2765050" target="_Blank">BBa_K2765050</a>
 +
                                    </td>
 +
                                    <td>TEF2p-roGFP2-Orp1-CYC1t</td>
 +
                                    <td>Detect ROS Content</td>
 +
                                    <td>1965 bp</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>14</td>
 +
                                    <td>
 +
                                        <a href="http://parts.igem.org/Part:BBa_K2765051" target="_Blank">BBa_K2765051</a>
 +
                                    </td>
 +
                                    <td>TEF1p-roGFP2-Orp1- CYC1t</td>
 +
                                    <td>Detect ROS Content</td>
 +
                                    <td>1964 bp</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>15</td>
 +
                                    <td>
 +
                                        <a href="http://parts.igem.org/Part:BBa_K2765052" target="_Blank">BBa_K2765052</a>
 +
                                    </td>
 +
                                    <td>ENO2p-roGFP2-Orp1- CYC1t</td>
 +
                                    <td>Detect ROS Content</td>
 +
                                    <td>2074 bp</td>
 +
                                </tr>
 +
                                <tr>
 +
                                    <td>16</td>
 +
                                    <td>
 +
                                        <a href="http://parts.igem.org/Part:BBa_K2765053" target="_Blank">BBa_K2765053</a>
 +
                                    </td>
 +
                                    <td>FBA1p-roGFP2-Orp1- CYC1t</td>
 +
                                    <td>Detect ROS Content</td>
 +
                                    <td>2193 bp</td>
 +
                                </tr>
 +
                            </tbody>
 +
                        </table>
 +
                    </div>
 +
                </div>
 +
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            <p style="font-size:15px;margin: 0;padding: 0;margin-top: 6px;">Institute of
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                Biotransformation and synthetic biosystem School of Chemistry and Chemical Engineering.
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            </p>
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            <p style="font-size:15px;margin: 0;padding: 0;margin-top: 6px;">
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                Beijing Institute of Technology
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            </p>
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            <p style="font-size:15px;margin: 0;padding: 0;margin-top: 6px;">
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                100081, Beijing
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                Email:lichun@bit.edu.cn
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Latest revision as of 22:20, 17 October 2018

PARTS

In 2018, BIT-China has submitted 16 parts. We divide our parts into 3 group, Regulator, Feedback and Output. Each group contains some parts with similar function or close relation. Different groups also connect with each other.

Regulator
Increase ROS Content

BBa_K2765026 and BBa_K2765021 is a collection of regulate components including coding protein and expression cassette, which can increase the accumulation of intracellular ROS through overexpressing yon1 induced by galactose.

Improve Tolerance

BBa_K2765030 is 1000bp homologous arm of yca1 gene, which can knock out yca1 gene through homologous recombination so that the yeast's tolerance to H2O2 could be improved.

Feedback
Control ROS Content

BBa_K2765000~BBa_K2765005 is a collection of feedback components that are downstream gene promoter of Yap1. We use them to turn on the expression of dCas9 which can inhibit the expression of yno1 gene. Considering the application of our system in the future, to realize different feedback effect, we verified different promoters, GLR1p, SOD2p, TRR1p, TRX2p and GSH1p, with different transcription level.

Output
Detect ROS content

BBa_K2765040~BBa_K2765053 is a collection of output components including promoters, coding protein and expression cassette, which can express redox-sensitive green fluorescent protein roGFP2-Orp1 and display ROS content in cells by its fluorescence ratio.

According to this measurement principle, the concentration of whole roGFP2-Orp1 can influence the final signal output strength, even detect the same sample, so we verified four promoters, FBA1, TEF2, TEF1 and ENO2, with different transcription level to solve this interfere. Considering the application of our system in the future, we made this collection and wished these parts can be used in different antioxidant detection.

Improve a previous part

We performed codon optimization on team Connell 2017's previous part BBa_K2296006 and obtained our core part BBa_K2296006, which codes for roGFP2-Orp1 protein. Also we got As a result, it is more suitable to our chassis organisms---yeast and has better expression in yeast. Check here to know further information.

Part Form
No. Biobrick Name Description Length
Regulator
1 BBa_K2765021 yno1 ROS Content Increaser 1713 bp
2 BBa_K2765026 GAL1p-yno1-CYC1t Increase ROS Content 2651 bp
3 BBa_K2765030 homologous arm-ura(△yca1) Knock out the yca1 gene 3038 bp
Feedback
4 BBa_K2765000 GAL1p Turn on the expression of dCas9 332 bp
5 BBa_K2765001 SOD2p Turn on the expression of dCas9 526 bp
6 BBa_K2765002 TRR1p Turn on the expression of dCas9 960 bp
7 BBa_K2765003 TRX2p Turn on the expression of dCas9 283 bp
8 BBa_K2765005 GSH1p Turn on the expression of dCas9 2027 bp
Output
9 BBa_K2765040 FBA1p Turn on the expression of roGFP2-Orp1 631 bp
10 BBa_K2765042 ENO2p Turn on the expression of roGFP2-Orp1 512 bp
11 BBa_K2765043 roGFP2-Orp1 ROS Content Detector 1317 bp
12 BBa_K2765045 TEF1p Turn on the expression of roGFP2-Orp1 402 bp
13 BBa_K2765050 TEF2p-roGFP2-Orp1-CYC1t Detect ROS Content 1965 bp
14 BBa_K2765051 TEF1p-roGFP2-Orp1- CYC1t Detect ROS Content 1964 bp
15 BBa_K2765052 ENO2p-roGFP2-Orp1- CYC1t Detect ROS Content 2074 bp
16 BBa_K2765053 FBA1p-roGFP2-Orp1- CYC1t Detect ROS Content 2193 bp

Contact

Institute of Biotransformation and synthetic biosystem School of Chemistry and Chemical Engineering.

Beijing Institute of Technology

100081, Beijing

Email:lichun@bit.edu.cn

Copyright © 2018 BIT-China