Difference between revisions of "Team:WashU StLouis/Daily"

 
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<body>
 
<body>
 
<div>
 
<div>
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<img src="https://static.igem.org/mediawiki/2018/b/b6/T--WashU_StLouis--resultsbanner.pngr.png" width="100%" style="top:40px;right:0px;"/>
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<img src="https://static.igem.org/mediawiki/2018/b/bb/T--WashU_StLouis--dailynotebookbanner.png" width="100%" style="top:40px;right:0px;"/>
 
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   <table>
 
   <table>
 
       <tr>
 
       <tr>
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         <th colspan=7 style="border:0.2vw solid #672A90    ;background-color:#3782B6; color:#3F0B76">July 2018</th>
+
         <th colspan=7 style="background-color:#3782B6; border: 0.2vw solid #386A8D; color:#04263E">July 2018</th>
 
       </tr>
 
       </tr>
 
       <tr>
 
       <tr>
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        <td class = "date">23</td>
 
 
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       <tr>
 
       <tr>
 +
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         <td class = "date">24</td>
 
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         <td class = "date">28</td>
 
         <td class = "date">29</td>
 
         <td class = "date">29</td>
−
        <td class = "date">30</td>
 
 
       </tr>
 
       </tr>
 
       <tr>
 
       <tr>
−
         <td></td>
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         <td class = "date">30</td>
 
         <td></td>
 
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     <p><em>Present: Cam, Diva, Elizabeth, Havisha</em></p>
 
     <p><em>Present: Cam, Diva, Elizabeth, Havisha</em></p>
 
     <ul>
 
     <ul>
−
         <li>Prepared chemically competent DH5α E. coli cells [Cam, Diva, Elizabeth, Havisha]</li>
+
         <li>Prepared chemically competent <I>DH5α</I> <I>E. coli</I> cells [Cam, Diva, Elizabeth, Havisha]</li>
 
         <li>Developed list of protocols for the wiki [Havisha, Diva]</li>
 
         <li>Developed list of protocols for the wiki [Havisha, Diva]</li>
 
     </ul>
 
     </ul>
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     <center>
 
     <center>
 
       <img src="https://static.igem.org/mediawiki/2018/2/25/T--WashU_StLouis--626notebook.jpeg" width= "300" />
 
       <img src="https://static.igem.org/mediawiki/2018/2/25/T--WashU_StLouis--626notebook.jpeg" width= "300" />
−
       <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Transformed DH5α from interlab studies day one on 6/25 </p>
+
       <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Transformed <I>DH5α</I> from interlab studies day one on 6/25 </p>
 
     </center>
 
     </center>
 
     </div>
 
     </div>
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         <li>Completed safety form [Diva, Havisha]</li>
 
         <li>Completed safety form [Diva, Havisha]</li>
 
         <li>Made LB Agar Media [Diva, Havisha]</li>
 
         <li>Made LB Agar Media [Diva, Havisha]</li>
−
         <li>Started an overnight culture of DH5α  [Diva, Havisha]</li>
+
         <li>Started an overnight culture of <I>DH5α</I> [Diva, Havisha]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
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         <li>Submitted safety form [Diva]</li>
 
         <li>Submitted safety form [Diva]</li>
 
         <li>Plated LB Agar plates [Diva, Elizabeth, Havisha]</li>
 
         <li>Plated LB Agar plates [Diva, Elizabeth, Havisha]</li>
−
         <li>Made chemically competent DH5α [Diva, Elizabeth, Havisha]</li>
+
         <li>Made chemically competent <I>DH5α</I> [Diva, Elizabeth, Havisha]</li>
 
         <li>Developed banners for wiki pages [Havisha]</li>
 
         <li>Developed banners for wiki pages [Havisha]</li>
 
     </ul>
 
     </ul>
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     <ul>
 
     <ul>
 
         <li>Performed calibration protocol for interlab study [Diva, Havisha]</li>
 
         <li>Performed calibration protocol for interlab study [Diva, Havisha]</li>
−
        <li>Made LB Agar + CM plates [Diva, Havisha]</li>
 
 
     </ul>
 
     </ul>
 
     <center>
 
     <center>
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     <p><em>Present: Diva, Elizabeth, Havisha</em></p>
 
     <p><em>Present: Diva, Elizabeth, Havisha</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Made LB Agar + CM plates [Diva, Elizabeth, Havisha]</li>
 +
        <li>Transformed <I>E. coli</I> <I>DH5α</I> for Interlab Protocol Day 1 [Diva, Elizabeth, Havisha]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
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     <p><em>Present: Diva, Elizabeth, Havisha</em></p>
 
     <p><em>Present: Diva, Elizabeth, Havisha</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Met with Jeff to discuss project goals and progress [Diva, Elizabeth, Havisha]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
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     <p><em>Present: Cam, Diva, Elizabeth</em></p>
 
     <p><em>Present: Cam, Diva, Elizabeth</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Worked on Day 2 of interlab protocol  [Cam, Diva, Elizabeth]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
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     <p><em>Present: Cam, Diva, Elizabeth</em></p>
 
     <p><em>Present: Cam, Diva, Elizabeth</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Worked on Day 3 of interlab protocol [Cam, Diva, Elizabeth]</li>
 +
        <li>Informed of contamination with E. coli and materials [Cam, Diva, Elizabeth]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
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     <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p>
 
     <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Made 1L LB Broth [Diva]</li>
 +
        <li>Made LB Agar + CM plates [Cam, Elizabeth]</li>
 +
        <li>Made TSS Buffer [Elizabeth, Diva]</li>
 +
        <li>Looked into GPCRs [Kyle]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
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     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><strong><u>Saturday, July 7</u></strong></p>
 
     <p><strong><u>Saturday, July 7</u></strong></p>
−
     <p>No work done today!</p>
+
     <p><em>Present: Cam, Elizabeth</em></p>
 +
    <ul>
 +
        <li>Transformed <I>DH5α</I> cells using competent cell test kit [Cam, Elizabeth]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><strong><u>Sunday, July 8</u></strong></p>
 
     <p><strong><u>Sunday, July 8</u></strong></p>
−
     <p>No work done today!</p>
+
     <p><em>Present: Elizabeth</em></p>
 +
    <ul>
 +
        <li>Checked transformed cell plates for growth [Elizabeth]</li>
 +
    </ul>
 +
    <center>
 +
      <img src="https://static.igem.org/mediawiki/2018/3/36/T--WashU_StLouis--781notebook.jpeg" width= "300" />
 +
      <img src="https://static.igem.org/mediawiki/2018/8/8e/T--WashU_StLouis--782notebook.jpeg" width= "300" />
 +
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Agar + CM plates with cells transformed with iGEM test kit from 7/7</p>
 +
    </center>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
 
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Consulted with Microsoft computing experts on modelling and device [Cam]</li>
 +
        <li>Finalized ribitol operon sequences to order [Havisha]</li>
 +
        <li>Studied resistance genes and sequences to order [Elizabeth]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
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     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
 
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Met with Dr. Brennan to discuss project checkpoints and progress [Elizabeth, Havisha, Kyle]</li>
 +
        <li>Made new LB media [Diva]</li>
 +
        <li>Made and plated LB agar and LB agar + CM [Diva, Elizabeth, Havisha]</li>
 +
        <li>Studied resistance genes and sequences to order [Elizabeth]</li>
 +
        <li>Ordered ribitol operon gene sequences [Havisha]</li>
 +
        <li>Met with Dr. Parikh and attended a lecture to develop international human practices [Kyle]</li>
 +
        <li>Began <I>DH5α</I> overnight culture [Diva, Elizabeth, Havisha]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
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     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><strong><u>Wednesday, July 11</u></strong></p>
 
     <p><strong><u>Wednesday, July 11</u></strong></p>
−
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
+
     <p><em>Present: Diva, Elizabeth, Havisha</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Plated <I>DH5α</I> cells on LB agar plate to check for plate contamination [Havisha]</li>
 +
        <li>Checked plated <I>DH5α</I> cells [Diva, Elizabeth]</li>
 
     </ul>
 
     </ul>
 +
    <center>
 +
      <img src="https://static.igem.org/mediawiki/2018/a/a4/T--WashU_StLouis--711notebook.jpeg" width= "300" />
 +
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Agar plate with overnight culture of <I>DH5α</I> from 7/11</p>
 +
    </center>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,019: Line 1,047:
 
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
 
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Conducted Day 1 of interlab protocol with competent cells from NEB [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
 +
        <li>Discussed human practices progress and integration into project [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
 +
        <li>Checked plated transformed <I>DH5α</I> cells [Diva, Elizabeth]</li>
 
     </ul>
 
     </ul>
 +
    <center>     
 +
      <img src="https://static.igem.org/mediawiki/2018/c/c8/T--WashU_StLouis--7121notebook.jpeg" width= "300" />   
 +
      <img src="https://static.igem.org/mediawiki/2018/5/5c/T--WashU_StLouis--7122notebook.jpeg" width= "300" />
 +
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Agar + CM plates with transformed cells from Day 1 of interlab protocol from 7/12</p>
 +
    </center>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p>
 
     <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Conducted Day 2 of interlab protocol [Diva, Elizabeth]</li>
 +
        <li>Met with Monsanto plant scientists to discuss project design and resistance genes [Diva, Elizabeth, Kyle]</li>
 +
        <li>Conducted Hour 0 of Day 3 of interlab protocol [Cam, Diva, Elizabeth]</li>
 +
        <li>Made LB Agar + CM plates [Cam, Diva]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
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     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><strong><u>Saturday, July 14</u></strong></p>
 
     <p><strong><u>Saturday, July 14</u></strong></p>
−
     <p>No work done today!</p>
+
     <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p>
 +
    <ul>
 +
        <li>Conducted Hour 6 of Day 3 of interlab protocol [Diva, Elizabeth, Kyle]</li>
 +
        <li>Made LB broth [Cam]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,057: Line 1,099:
 
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
 
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Conducted a production meeting to redefine project progress and delegate individual tasks [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
 +
        <li>Compiling gene sequences for resistance genes [Elizabeth]</li>
 +
        <li>Met with a representative from the Missouri Coalition for the Environment [Kyle]</li>
 +
        <li>Worked on device logistics and reached out to possible advisors for development [Kyle]</li>
 +
        <li>Procured space in WashU makerspace for device prototyping [Cam]</li>
 +
        <li>Solidified wiki homepage formatting [Havisha]</li>
 +
        <li>Looked into longevity of E. coli and yeast and possible alternatives to maintaining a live culture in device [Diva]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
Line 1,068: Line 1,116:
 
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
 
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Researched biobrick scars and Gibson assembly as a possible alternative [Elizabeth]</li>
 +
        <li>Finalized gene sequences for resistance genes [Elizabeth]</li>
 +
        <li>Began developing poster for EECE interns presentation [Havisha]</li>
 +
        <li>Ordered ribitol for testing transformed cells [Cam]</li>
 +
        <li>Contacted Bayer for possible collaborations with education and human practices [Kyle]</li>
 +
        <li>Worked on device design [Kyle]</li>
 +
        <li>Explored powdered cells for one time use as an alternative to a live culture in device [Diva]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
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     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
 
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Worked on poster for presentation [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
 +
        <li>Worked on summary presentation for video conference [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
 +
        <li>Looked into plasmids and promoter to use with ribitol operon [Havisha]</li>
 +
        <li>Ordered AvrSr35 gene sequences [Elizabeth]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
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     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
 
     <p><strong><u>Thursday, July 19</u></strong></p>
 
     <p><strong><u>Thursday, July 19</u></strong></p>
−
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
+
     <p><em>Present: Cam, Elizabeth, Havisha, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Attended and presented at Southern iGEM video conference [Elizabeth, Havisha, Kyle]</li>
 +
        <li>Made Agar + CM plates [Cam, Havisha, Kyle]</li>
 +
        <li>Discussed and consulted grad students regarding golden gate assembly for resistance gene sequences [Cam, Elizabeth, Havisha, Kyle]</li>
 +
        <li>Worked on poster for presentation [Havisha]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
Line 1,101: Line 1,161:
 
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
 
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
 
     <ul>
 
     <ul>
−
         <li>ploop</li>
+
         <li>Met with the head of St. Louis Indoor Produce and toured facility for possible collaborations and human practices [Diva, Elizabeth, Havisha, Kyle]</li>
 +
        <li>Met to discuss and define project timeline and goals [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
 
     </ul>
 
     </ul>
 
     </div>
 
     </div>
Line 1,109: Line 1,170:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Friday, July 21</u></strong></p>
+
     <p><strong><u>Saturday, July 21</u></strong></p>
−
     <p><em>Present: Alex, Collin, Zoe, Maddie, Micah, Mark</em></p>
+
     <p><em>Present: Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Conducted a collaborations video call with the ICT Mumbai iGEM team [Elizabeth, Kyle]</li>
−
         <li>Miniprepped the three cultures with lac+RBS+phrAT <em>Alex</em></li>
+
         <li>Attended and presented at North American Kick-off event video conference [Elizabeth, Havisha, Kyle]</li>
−
        <li>Measured the OD730 of the four cyanobacteria cultures <em>[Maddie]</em></li>
+
        <li>Transformed <I>DH5α</I> with positive and negative controls for interlab CFU/mL protocol [Diva, Havisha]</li>
−
              <ul>
+
         <li>Checked plates and began four overnight cultures for interlab CFU/mL protocol [Diva]</li>
−
                  <li>0.9366 (#1), 3.989 (#2), 1.584 (#3), 0.6518 (#4)</li>
+
     </ul>
−
              </ul>
+
     <center>      
−
         <li>Measured the amount of light that each cyanobacteria is receiving on average (in micro-molars per meter squared per second) using a FieldScout Quantum Light Meter <em>[Maddie, Zoe]</em></li>
+
      <img src="https://static.igem.org/mediawiki/2018/4/4b/T--WashU_StLouis--7211notebook.jpeg" width= "30%" />  
−
              <ul>
+
      <img src="https://static.igem.org/mediawiki/2018/5/53/T--WashU_StLouis--7212notebook.jpeg" width= "30%" />
−
                  <li> 8.4 (#1), 23 (#2), 16.6 (#3), 9.8 (#4)</li>
+
      <img src="https://static.igem.org/mediawiki/2018/d/d3/T--WashU_StLouis--7213notebook.jpeg" width= "30%" />
−
              </ul>
+
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Agar + CM plates with transformed cells from for CFU interlab protocol from 7/21</p>
−
        <li>Digested lac+RBS+uvsE, lac+RBS+phrAC, lac+RBS+Dsup, and RBS+blue chromoprotein to determine if the ligations were correct <em>[Alex, Mark]</em></li>
+
    </center>
−
         <li>Performed gel electrophoresis on the 3 miniprepped samples (#4, #5, #6) of phrAT and the digested lac+RBS+uvsE, lac+RBS+phrAC, lac+RBS+Dsup, and RBS+blue chromoprotein to test if the ligations were correct <em>[Alex]</em></li>
+
−
     </ol>
+
−
     <p><strong> Outside Work / Discussion </strong><p>
+
−
    <ol>
+
−
        <li>Skyped with Cardiff iGEM to explore potential collaboration scenarios <em>[Alex, Micah, Mark, Zoe, Collin]</em></li>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,134: Line 1,190:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Saturday, July 22</u></strong></p>
+
     <p><strong><u>Sunday, July 22</u></strong></p>
−
     <p><em></em></p>
+
     <p><em>Present: Diva, Havisha</em></p>
−
     <p>No lab work done today!</p>
+
     <ul>
 +
        <li>Conducted dilution and plating steps for interlab CFU/mL protocol [Diva, Havisha]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,143: Line 1,201:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Sunday, July 23</u></strong></p>
+
     <p><strong><u>Monday, July 23</u></strong></p>
−
     <p><em>Present: Maddie</em></p>
+
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Conducted a production meeting to redefine project progress and delegate individual tasks [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
−
         <li>Measured the OD730 of the four cyanobacteria cultures <em>[Maddie]</em></li>
+
        <li>Met with Dr. Feher to discuss device logistics [Kyle]</li>
−
              <ul>
+
        <li>Counted colonies on plates for interlab CFU/mL protocol [Cam, Diva, Elizabeth, Kyle]</li>
−
                  <li> 1.4122 (#1), 3.965 (#2), 2.3244 (#3), 0.9878 (#4)</li>
+
         <li>Met with Microsoft to touch base on goals for the modeling component of our project [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
−
              </ul>
+
        <li>Met with Dr. Brennan to discuss part assembly and testing of ordered parts [Diva, Elizabeth, Havisha]</li>
−
    </ol>
+
    </ul>
−
    <p><strong> Outside Work / Discussion </strong><p>
+
    <center>      
 +
      <img src="https://static.igem.org/mediawiki/2018/a/a4/T--WashU_StLouis--723.1notebook.jpeg" width= "30%" />  
 +
      <img src="https://static.igem.org/mediawiki/2018/0/05/T--WashU_StLouis--723.2notebook.jpeg" width= "30%" />
 +
      <img src="https://static.igem.org/mediawiki/2018/0/0d/T--WashU_StLouis--723.3notebook.jpeg" width= "30%" />
 +
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Agar + CM plates with diluted cultures of transformed cells from for CFU interlab protocol from 7/22</p>
 +
    </center>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,159: Line 1,222:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Monday, July 24</u></strong></p>
+
     <p><strong><u>Tuesday, July 24</u></strong></p>
−
     <p><em>Present: Maddie, Micah, Mark, Zoe, Collin, Alex</em></p>
+
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Toured the Danforth Plant Sciences Center [Cam, Elizabeth, Havisha, Kyle]</li>
−
         <li>Digested lac+RBS+uvsE, lac+RBS+phrAC, lac+RBS+Dsup, phrAT, lac+RBS and RBS+blue chromoprotein <em>[Alex]</em></li>
+
         <li>Finished and printed poster [Diva]</li>
−
        <li>Performed gel electrophoresis for the digested lac+RBS+uvsE, lac+RBS+phrAC, lac+RBS+Dsup, phrAT, lac+RBS and RBS+blue chromoprotein <em>[Alex]</em></li>
+
    </ul>
−
        <li>Measured the OD730 of the four cyanobacteria cultures <em>[Mark, Micah]</em></li>
+
−
              <ul>
+
−
                  <li> 1.7252 (#1), 3.884 (#2), 2.8400 (#3), 1.218 (#4)</li>
+
−
              </ul>
+
−
        <li>Digested uvsE, phrAC, Dsup, and lac+RBS <em>[Collin, Mark]</em></li>
+
−
         <li>Performed gel electrophoresis on digested uvsE, phrAC, Dsup, and lac+RBS <em>[Mark, Collin]</em></li>
+
−
        <li>Performed a PCR gradient test on phrAT, phrAC, Dsup, and uvsE with Golden Gate compatible primers <em>[Maddie, Zoe]</em></li>
+
−
              <ul>
+
−
                  <li>uvsE and Dsup were successful</li>
+
−
              </ul>
+
−
        <li>Prepared three overnight cultures of lac+RBS+phrAC, three cultures of lac+RBS+uvsE, and two cultures of lac+RBS+Dsup <em>[Zoe]</em></li>
+
−
 
+
−
    </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
−
    <ol>
+
−
        <li>Practiced our presentation for the EECE poster session <em>[Maddie, Alex, Collin]</em></li>
+
−
        <li>Met with Dr. Brennan to discuss our progress <em>[Mark, Maddie, Alex, Collin, Zoe, Micah]</em></li>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Tuesday, July 25</u></strong></p>
+
     <p><strong><u>Wednesday, July 25</u></strong></p>
−
     <p><em>Present: Micah, Alex, Collin, Zoe, Mark, Maddie</em></p>
+
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Presented at EECE Interns Poster Session [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
−
         <li>Made glycerol stocks of three cultures with lac+RBS+phrAC (#4, #5, #6), three cultures with lac+RBS+uvsE (#4, #5, #6), and two cultures with lac+RBS+Dsup (#4, #5) <em>[Alex]</em></li>
+
         <li>Met with Dr. Kunkel to discuss resistance genes and application of project [Elizabeth, Havisha]</li>
−
        <li>Performed gel purification on the Golden Gate (GG) compatible uvsE and Dsup PCR products <em>[Maddie, Mark]</em></li>
+
         <li>Ordered Sr35 genes [Elizabeth]</li>
−
        <li>Performed gel purification on lac+RBS, phrAC, Dscup, and uvsE <em>[Mark, Maddie]</em></li>
+
     </ul>  
−
         <li>Miniprepped three cultures with lac+RBS+phrAC (#4, #5, #6), three cultures with lac+RBS+uvsE (#4, #5, #6), and two cultures with lac+RBS+Dsup (#4, #5) <em>[Zoe]</em></li>
+
  </div>
−
        <li>Measured the OD730 of the four cyanobacteria colonies <em>[Mark, Collin]</em></li>
+
−
              <ul>
+
−
                  <li> 2.126 (#1), 3.881 (#2), 3.667 (#3), 1.5272 (#4)</li>
+
−
              </ul>
+
−
         <li>Transformed BBa_I13521 (RFP with a constitutive promoter) into DH5α E. coli cells <em>[Maddie]</em></li>
+
−
        <li>Ligated lac+RBS (BBa_J04500) with phrAC, Dsup, phrAT, and uvsE individually in pSB1C3 <em>[Alex, Mark, Collin]</em>
+
−
        <li>Transformed the ligated lac+RBS+Dsup, lac+RBS+phrAT, lac+RBS+phrAC, and lac+RBS+uvsE <em>[Mark, Collin]</em></li>
+
−
       
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
−
    <ol>
+
−
        <li>Presented at the EECE summer poster session <em>[Zoe, Maddie, Collin, Alex, Micah, Mark]</em></li>
+
−
    </ol>
+
−
    </div>
+
 
   </div>
 
   </div>
  
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Wednesday, July 26</u></strong></p>
+
     <p><strong><u>Thursday, July 26</u></strong></p>
−
     <p><em>Present: Maddie, Mark Wang, Collin, Alex, Zoe, Micah</em></p>
+
     <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Made ampicillin and chloramphenicol [Cam]</li>
−
         <li>Measured the OD730 of the four cyanobacteria cultures <em>[Collin, Mark]</em></li>
+
         <li>Made kanamycin [Elizabeth]</li>
−
              <ul>
+
         <li>Made LB+Cam plates [Kyle]</li>
−
                  <li> 2.2204 (#1), 3.742 (#2), 3.786 (#3), 1.7132 (#4)
+
         <li>Made overnight <I>DH5α</I> cultures [Diva]</li>
−
              </ul>
+
     </ul>
−
         <li>Performed gradient PCR on PCV0020 <em>[Zoe, Maddie]</em></li>
+
−
              <ul>
+
−
                  <li> Did not work </li>
+
−
              </ul>
+
−
         <li>Made liquid cultures for 3 colonies each of lac+RBS+uvsE, lac+RBS+Dsup, lac+RBS+phrAC, lac+RBS+phrAT (#7, #8, #9) <em>[Alex]</em></li>
+
−
        <li>Tested the efficacy of the UV box by exposing plates of DH5α E. coli cells with RFP to UV for different time intervals <em>[Micah, Mark]</em></li>
+
−
         <li>Plated pDeg+PCPC360 and pDeg+pTrc on Kan plates and PCV0020 on Spec plates <em>[Collin, Mark]</em></li>
+
−
    </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
−
    <ol>
+
−
        <li>Met with Dr. Larry Gilbertson, a molecular biologist at Monsanto, and discussed methods of transforming plant cells <em>[Alex, Micah, Mark, Collin, Zoe]</em></li>
+
−
     </ol>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Thursday, July 27</u></strong></p>
+
     <p><strong><u>Friday, July 27</u></strong></p>
−
     <p><em>Present: Zoe, Micah, Alex, Collin, Mark</em></p>
+
     <p><em>Present: Diva, Elizabeth, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Made glycerol stocks of <I>DH5α</I> cells [Diva]</li>
−
        <li>Checked the UV-exposed RFP plates <em>[Micah]</em></li>
+
         <li>Made competent <I>DH5α</I> cells [Diva, Elizabeth, Kyle]</li>
−
         <li>Made glycerol stocks for three cultures each of lac+RBS+phrAC, lac+RBS+phrAT, lac+RBS+uvsE, and lac+RBS+Dsup (#7, #8, #9) <em>[Alex]</em></li>
+
     </ul>
−
        <li>Miniprepped the three cultures each of lac+RBS+phrAC, lac+RBS+phrAT, lac+RBS+uvsE, and lac+RBS+Dsup (#7, #8, #9) <em>[Zoe, Mark]</em>  </li>
+
−
         <li>Prepared Kanamycin (Kan) and Spectinomycin (Spec) solutions <em>[Mark, Collin]</em></li>
+
−
        <li>Measured the OD730 of the four cyanobacteria cultures <em>[Mark]</em></li>
+
−
              <ul>
+
−
                  <li> 2.4388 (#1), 0.3345 (#2), 0.3613 (#3), 1.9760 (#4)</li>
+
−
              </ul>
+
−
        <li>Tested the UV box and shaker with two cultures of DH5α E. coli cells with RFP (One inside the box and the other outside the box) <em>[Micah]</em></li>
+
−
        <li>Prepared three overnight cultures of DH5α E. coli cells with pDeg+PCPC360, pDeg+pTrc, and PCV0020 <em>[Collin]</em></li>
+
−
        <li>Digested lac+RBS+Dsup #7, lac+RBS+uvsE #7, lac+RBS+phrAC #8, lac+RBS+phrAT #9, and RBS+blue chromoprotein #1 <em>[Alex]</em></li>
+
−
        <li>Performed gel electrophoresis for the digested lac+RBS+Dsup #7, lac+RBS+uvsE #7, lac+RBS+phrAC #8, lac+RBS+phrAT #9, and RBS+blue chromoprotein #1 <em>[Alex]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Friday, July 28</u></strong></p>
+
     <p><strong><u>Saturday, July 28</u></strong></p>
−
     <p><em>Present: Collin, Mark, Zoe, Alex, Micah</em></p>
+
     <p><em>Present: Elizabeth, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Ran competent cell test kit [Elizabeth, Kyle]</li>
−
         <li>Checked the OD600 of the two cultures used to test the UV box and shaker <em>[Micah]</em></li>
+
         <li>Discussed human practices [Elizabeth, Kyle]</li>
−
        <li>Prepared glycerol stocks of DH5α E. coli cells with pDeg+PCPC360, pDeg+pTrc, and PCV0020 <em>[Alex]</em></li>
+
    </ul>
−
         <li>Miniprepped the DH5α E. coli cells with pDeg+PCPC360, pDeg+pTrc, and PCV0020 <em>[Zoe]</em></li>
+
−
        <li>Measured the OD730 of the cyanobacteria cultures <em>[Mark, Collin]</em></li>
+
−
              <ul>
+
−
                  <li>2.8328 (#1), 3.682 (#2), 3.652 (#3), 2.2800 (#4)</li>
+
−
              </ul>
+
−
        <li>Performed a PCR gradient for phrAT and phrAC with GG compatible primers <em>[Alex]</em></li>
+
−
        <li>Performed gel electrophoresis for the PCR gradient for phrAT and phrAC with GG compatible primers <em>[Alex]</em></li>
+
−
    </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Saturday, July 29</u></strong></p>
+
     <p><strong><u>Sunday, July 29</u></strong></p>
−
     <p><em>Present: Micah, Collin</em></p>
+
     <p><em>Present: Diva, Elizabeth, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Checked plates from competent cell kit [Elizabeth]</li>
−
        <li>Measured the OD600 of the DH5α E. coli cultures (one inside the box and one in the culture room) for the UV box test <em>[Micah]</em></li>
+
        <li>Worked on wiki content for global human practices [Diva, Kyle]</li>
−
        <li>Measured the OD730 of the four cyanobacteria cultures <em>[Collin]</em></li>
+
    </ul>
−
            <ul>
+
−
                <li>3.0176 (#1), 3.622 (#2), 3.468 (#3), 2.2704 (#4)</li>
+
−
            </ul>
+
−
    </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Sunday, July 30</u></strong></p>
+
     <p><strong><u>Monday, July 30</u></strong></p>
−
     <p><em></em></p>
+
     <p><em>Present: Diva, Elizabeth, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Grew an overnight culture of <I>DH5α</I> cells [Diva]
−
         <li>Measured the OD730 of the four cyanobacteria cultures <em>[Mark]</em></li>
+
        <li>Transformed NEB competent <I>DH5α</I> cells with pSB1C3 (pre-miniprep) [Elizabeth]</li>
−
              <ul>
+
         <li>Tested for competent cells [Kyle]</li>
−
                  <li>3.3316 (#1), 3.489 (#2), 3.314 (#3), 2.7836 (#4)</li>
+
         <li>Met with Dr. Brennan to discuss part assembly and competent cell preparation protocol [Diva, Elizabeth, Kyle]</li>
−
              </ul>
+
         <li>Met with Jeff and other grad students to discuss preventing contamination and improving efficacy of interlab technique [Diva, Elizabeth, Kyle]</li>
−
         <li>Prepared overnight cultures of DH5α E. coli cells with lac+RBS+Dsup, lac+RBS+phrAT, and RFP individually <em>[Mark]</em></li>
+
     </ul>
−
         <li>Digested uvsE, phrAT, phrAC, and Dsup from pSB1C3 and digested lac+RBS+psB1A2 to linearize the plasmid <em>[Alex]</em></li>
+
−
         <li>Performed gel electrophoresis for uvsE, phrAT, phrAC, Dsup, and lac+RBS+psB1A2 <em>[Alex]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Monday, July 31</u></strong></p>
+
     <p><strong><u>Tuesday, July 31</u></strong></p>
−
    <p><em>Present: Collin, Micah, Alex, Mark, Zoe</em></p>
+
    <p><em>Present: Diva, Elizabeth, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Made competent <I>DH5α</I> cells [Kyle, Elizabeth]</li>
−
        <li>Performed a PCR gradient for phrAT and phrAC with GG compatible primers <em>[Mark, Collin]</em></li>
+
         <li>Tested TSS and SOC media pH with glass pH probe [Elizabeth, Diva]</li>
−
         <li>Performed gel electrophoresis for the PCR gradient of phrAT and phrAC (with GG compatible primers) <em>[Mark]</em></li>
+
    </ul>
−
        <li>Measured the OD730 of the four cyanobacteria cultures <em>[Mark]</em></li>
+
    <center>      
−
              <ul>
+
      <img src="https://static.igem.org/mediawiki/2018/c/c4/T--WashU_StLouis--7311notebook.jpeg" width= "30%" />  
−
                  <li>3.913 (#1), 3.371 (#2), 3.357 (#3), 3.273 (#4)</li>
+
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Cells transformed with pSB1C3 from 7/30</p>
−
              </ul>
+
    </center>
−
        <li>Prepared a liquid culture of DH5α cells wih lac+RBS+phrAT in pSB1C3 <em>[Zoe]</em></li>
+
    <center>      
−
        <li>Ligated phrAT, Dsup, phrAC, and uvsE into a pSB1A2 backbone with lac+RBS <em>[Zoe]</em></li>
+
      <img src="https://static.igem.org/mediawiki/2018/5/55/T--WashU_StLouis--7312notebook.jpeg" width= "20%" />  
−
        <li>Performed another PCR gradient for phrAT  with GG compatible ends <em>[Collin]</em></li>
+
      <img src="https://static.igem.org/mediawiki/2018/5/52/T--WashU_StLouis--7313notebook.jpeg" width= "50%" />  
−
        <li>Performed gel electrophoresis for the PCR gradient of phrAT with GG compatible ends <em>[Collin, Mark]</em></li>
+
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Results from competent cell test kit from 7/30</p>
−
        <li>Digested lac+RBS+Dsup+pSB1C3 and lac+RBS+phrAT+pSB1C3 to linearize the plasmids <em>[Micah]</em></li>
+
    </center>
−
        <li>Performed gel electrophoresis on the digested lac+RBS+Dsup+pSB1C3 and lac+RBS+phrAT+pSB1C3 plasmids <em>[Mark]</em></li>
+
−
    </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,346: Line 1,333:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Tuesday, August 1</u></strong></p>
+
     <p><strong><u>Wednesday, August 1</u></strong></p>
−
     <p><em>Present: Zoe, Mark, Micah, Collin, Alex</em></p>
+
     <p><em>Present: Diva</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Made LB agar plates [Diva]</li>
−
        <li>Miniprepped lac+RBS+phrAT+pSB1C3 <em>[Zoe]</em></li>
+
     </ul>
−
        <li>Purified RBS+blue chromoprotein and the linearized lac+RBS+Dsup+pSB1C3 from their respective gels <em>[Mark]</em></li>
+
−
        <li>Digested PCPC360 and pTrc to linearize both plasmids <em>[Micah]</em></li>
+
−
        <li>Performed gel electrophoresis on the digested PCPC360 and pTrc <em>[Micah, Mark]</em></li>
+
−
        <li>Digested lac+RBS+phrAT+pSB1C3 <em>[Micah]</em></li>
+
−
        <li>Performed gel electrophoresis on the digested lac+RBS+phrAT+pSB1C3 along with undigested PCPC360 and pTrc <em>[Mark]</em></li>
+
−
        <li>Measured the OD730 of the four cyanobacteria cultures <em>[Micah]</em></li>
+
−
              <ul>
+
−
                  <li>4.078 (#1), 3.298 (#2), 3.163 (#3), 3.422 (#4)</li>
+
−
              </ul>
+
−
         <li>Ligated RBS+Blue chromoprotein into lac+RBS+Dsup+pSB1C3 <em>[Mark]</em></li>
+
−
        <li>Transformed the ligated lac+RBS+Dsup+RBS+Blue chromoprotein+psB1C3 into DH5α E. coli cells <em>[Mark, Zoe]</em></li>
+
−
        <li>Prepared three cultures each of DH5α E. coli cells with lac+RBS+uvsE+psB1A2, lac+RBS+Dsup+psB1A2, lac+RBS+phrAT+pSB1A2, and lac+RBS+phrAC+pSB1A2 (numbered #1, #2, #3) <em>[Micah]</em></li>
+
−
        <li>Prepared an overnight cultures of DH5α E. coli cells <em>[Collin]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Wednesday, August 2</u></strong></p>
+
     <p><strong><u>Thursday, August 2</u></strong></p>
−
     <p><em>Present: Zoe, Mark, Micah, Collin, Alex</em></p>
+
     <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Made competent <I>DH5α</I> cells [Cam, Diva]</li>
−
         <li>Prepared glycerol stocks for each culture of DH5α E. coli cells with lac+RBS+uvsE+psB1A2, lac+RBS+Dsup+psB1A2, lac+RBS+phrAT+pSB1A2, and lac+RBS+phrAC+pSB1A2 (numbered #1, #2, #3) <em>[Mark]</em></li>
+
         <li>Transformed competent cells with RFP [Kyle, Elizabeth]</li>
−
        <li>Miniprepped each culture of DH5α E. coli cells with lac+RBS+uvsE+psB1A2, lac+RBS+Dsup+psB1A2, lac+RBS+phrAT+pSB1A2, and lac+RBS+phrAC+pSB1A2 (numbered #1, #2, #3) <em>[Zoe]</em></li>
+
     </ul>
−
         <li>Measured the OD730 of the four cyanobacteria cultures <em>[Mark]</em></li>
+
−
            <ul>
+
−
                <li>4.783 (#1), 3.230 (#2), 3.110 (#3), 4.019 (#4)</li>
+
−
            </ul>
+
−
        <li>Prepared an overnight culture of a DH5α E. coli colony with ligated lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 <em>[Micah]</em></li>
+
−
        <li>Digested the previously ligated lac+RBS+phrAT+pSB1A2, lac+RBS+Dsup+pSB1A2, lac+RBS+phrAC+pSB1A2, and lac+RBS+uvsE+pSB1A2 to test if the ligations were correct <em>[Mark, Micah]</em></li>
+
−
        <li>Performed gel electrophoresis on the digested lac+RBS+phrAT+pSB1A2, lac+RBS+Dsup+pSB1A2, lac+RBS+phrAC+pSB1A2, and lac+RBS+uvsE+pSB1A2 <em>[Mark]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Thursday, August 3</u></strong></p>
+
     <p><strong><u>Friday, August 3</u></strong></p>
−
     <p><em>Present: Zoe, Mark, Micah, Collin, Alex</em></p>
+
     <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Transformed competent cells with Part BBa_K2663000 [Elizabeth, Kyle]</li>
−
         <li>Miniprepped the lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 <em>[Zoe]</em></li>
+
         <li>Made LB agar + CM plates [Diva, Cam]</li>
−
        <li>Digested the pCB3C5 backbone, lac+RBS+Dsup+pSB1A2, lac+RBS+phrAT+pSB1A2, lac+RBS+phrAC+pSB1A2, lac+RBS+uvsE+pSB1A2, and RBS+Blue chromoprotein <em>[Collin]</em></li>
+
         <li>Made overnight cultures of cells transformed with RFP [Cam]</li>
−
         <li>Measured the OD730 of the four cyanobacteria cultures <em>[Mark]</em></li>
+
     </ul>
−
              <ul>
+
−
                  <li>5.052 (#1), 3.208 (#2), 3.156 (#3), 4.308 (#4)</li>
+
−
              </ul>
+
−
        <li>Performed gel electrophoresis on the digested pCB3C5 backbone, lac+RBS+Dsup+pSB1A2, lac+RBS+phrAT+pSB1A2, lac+RBS+phrAC+pSB1A2, lac+RBS+uvsE+pSB1A2, and RBS+Blue chromoprotein <em>[Mark, Collin]</em></li>
+
−
         <li>Digested the previously ligated lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 to test if the ligation was correct<em>[Alex]</em></li>
+
−
        <li>Performed gel electrophoresis on the digested lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 <em>[Alex]</em></li>
+
−
        <li>Prepared three overnight cultures of DH5α E. coli cells with lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 and with lac+RBS+phrAT+pSB1A2 <em>[Micah]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Friday, August 4</u></strong></p>
+
     <p><strong><u>Saturday, August 4</u></strong></p>
−
     <p><em>Present: Zoe, Mark, Micah, Collin, Alex</em></p>
+
     <p><em>Present: Elizabeth, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Made LB [Elizabeth, Kyle]</li>
−
         <li>Miniprepped three cultures of lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 (#2, #3, #4) and three cultures of lac+RBS+phrAT+pSB1A2 (#1, #2, #3) <em>[Zoe]</em></li>
+
         <li>Made glycerol stocks of overnight cultures [Elizabeth, Kyle]</li>
−
         <li>Measured the OD730 of the four cyanobacteria cultures <em>[Mark]</em></li>
+
         <li>Plated cells to check for contamination [Elizabeth, Kyle]</li>
−
              <ul>
+
        <li>Ordered miniprep kit [Elizabeth, Kyle]</li>
−
                  <li>5.207 (#1), 3.122 (#2), 3.024 (#3), 4.709 (#4)</li>
+
        <li>Checked plates to ensure lack of contamination [Elizabeth, Kyle]</li>
−
              </ul>
+
         <li>Plated overnight cultures [Elizabeth, Kyle]</li>
−
         <li>Prepared overnight cultures of DH5α E. coli cells with lac+RBS+uvsE+pSB1A2 and lac+RBS+phrAC+pSB1A2 <em>[Mark]</em></li>
+
     </ul>
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Saturday, August 5</u></strong></p>
+
     <p><strong><u>Sunday, August 5</u></strong></p>
−
     <p><em>Present: Zoe, Mark, Micah, Collin, Alex</em></p>
+
     <p><em>Present: Elizabeth</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Made glycerol stocks from overnight cultures [Elizabeth]</li>
−
         <li>Measured the OD730 of the four cyanobacteria cultures <em>[Collin]</em></li>
+
        <li>Miniprepped RFP, ribitol component promoter, and RBS [Elizabeth]</li>
−
              <ul>
+
         <li>Autoclaved new tips and microcentrifuge tubes [Elizabeth]</li>
−
                  <li>7.436 (#1), 3.031 (#2), 2.914 (#3), 4.865 (#4)</li>
+
         <li>Made a negative control to test for contamination in the culture tubes [Elizabeth]</li>
−
              </ul>
+
     </ul>
−
         <li>Miniprepped lac+RBS+uvsE+pSB1A2 and lac+RBS+phrAC+pSB1A2 <em>[Collin]</em></li>
+
−
         <li>Plated DH5α E. coli cells with lac+RBS+Dsup+RBS+Blue chromoprotein+pSB1C3 from its glycerol stock <em>[Collin]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Sunday, August 6</u></strong></p>
+
     <p><strong><u>Monday, August 6</u></strong></p>
−
     <p><em>Present: Zoe, Mark, Micah, Collin, Alex</em></p>
+
     <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Made ampicillin plates [Cam, Diva]</li>
−
         <li>Measured the OD730 of the four cyanobacteria cultures <em>[Mark]</em></li>
+
        <li>Measured DNA concentrations using nanodrop [Cam, Diva, Elizabeth, Kyle]</li>
−
              <ul>
+
         <li>Made more ampicillin, chloramphenicol, and kanamycin [Cam, Diva]</li>
−
                  <li>5.473 (#1), 3.029 (#2), 2.650 (#3), 4.676 (#4)</li>
+
         <li>Plated pRSII424 on ampicillin plate [Cam, Diva]</li>
−
              </ul>
+
     </ul>
−
        <li>Prepared an overnight cultures from the glycerol stock of DH5α E. coli cells with lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 <em>[Mark]</em></li>
+
−
         <li>Digested lac+RBS+phrAC+pSB1A2 (3 samples: #4, #5, and #6), lac+RBS+uvsE+pSB1A2 (3 samples: #4, #5, and #6), lac+RBS+pSB1A2, phrAT+pSB1C3, phrAC+pSB1C3, uvsE+psB1C3, lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3, pSB4C5, and pSB3C5 <em>[Collin, Mark]</em></li>
+
−
         <li>Performed gel electrophoresis on the digested lac+RBS+phrAC+pSB1A2 (3 samples: #4, #5, and #6), lac+RBS+uvsE+pSB1A2 (3 samples: #4, #5, and #6), lac+RBS+pSB1A2, phrAT+pSB1C3, phrAC+pSB1C3, uvsE+psB1C3, lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3, pSB4C5, and pSB3C5 <em>[Collin]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Monday, August 7</u></strong></p>
+
     <p><strong><u>Tuesday, August 7</u></strong></p>
−
     <p><em>Present: Zoe, Mark, Micah, Maddie, Collin, Alex</em></p>
+
     <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Made an overnight culture of pRSII424 [Cam, Elizabeth, Kyle]</li>
−
         <li>Measured the OD730 of the four cyanobacteria cultures <em>[Maddie]</em></li>
+
     </ul>
−
              <ul>
+
−
                  <li>5.820 (#1), 2.935 (#2), 2.564 (#3), 4.845 (#4)</li>
+
−
              </ul>
+
−
        <li>Purified the digested uvsE, phrAC, lac+RBS+psB1A2, pSB4C5, and phrAT from their respective agarose gels <em>[Collin]</em></li>
+
−
        <li>Miniprepped lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 <em>[Micah]</em></li>
+
−
        <li>Prepared competent DH5α E. coli cells <em>[Micah, Maddie]</em></li>
+
−
        <li>Transformed DH5α E. coli cells with RFP (BBa_J00450) <em>[Maddie, Micah]</em></li>
+
−
        <li>Ligated uvsE to lac+RBS+pSB1A2, phrAC to lac+RBS+pSB1A2, and lac+RBS+Dsup+RBS+blue chromoprotein to pSB4C5 <em>[Collin]</em></li>
+
−
        <li>Transformed DH5α E. coli cells with the ligated lac+RBS+pSB1A2, lac+RBS+phrAC+pSB1A2, and lac+RBS+Dsup+RBS+blue chromoprotein+pSB4C5 <em>[Collin]</em></li>
+
−
        <li>Digested phrAT+pSB1C3, lac+RBS+uvsE+pSB1A2 #5, pSB1C3+RBS+blue chromoprotein, and lac+pSB1C3 <em>[Mark]</em></li>
+
−
        <li>Performed gel electrophoresis on the digested phrAT+pSB1C3, lac+RBS+uvsE+pSB1A2 #5, pSB1C3+RBS+blue chromoprotein, and lac+pSB1C3 <em>[Mark]</em></li>
+
−
        <li>Prepared LB Media <em>[Maddie]</em></li>
+
−
        <li>Performed the UV irradiation test on DH5α cells with Dsup (with time intervals of 0, 2, 5, and 15 minutes) <em>[Micah, Alex]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Tuesday, August 8</u></strong></p>
+
     <p><strong><u>Wednesday, August 8</u></strong></p>
−
     <p><em>Present: Zoe, Mark, Micah, Maddie, Collin, Alex</em></p>
+
     <p><em>Present: Elizabeth, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Minipreped pRSII424 plasmid [Elizabeth, Havisha, Kyle]</li>
−
         <li>Purified lac+RBS+uvsE+pSB1A2, RBS+blue chromoprotein, lac+pSB1C3, and lac+RBS+uvsE from their respective agarose gels <em>[Collin]</em></li>
+
         <li>Performed PCR on ribitol operon, ribitol transporter, and AvrSr35 [Elizabeth, Havisha, Kyle]</li>
−
         <li>Performed PCR on phrAT with Golden Gate (GG) compatible primers (Step 1) <em>[Maddie]</em></li>
+
         <li>Began an overnight culture of <I>DH5α</I> cells [Elizabeth, Havisha]</li>
−
        <li>Performed gel electrophoresis on the GG compatible phrAT PCR product <em>[Maddie]</em></li>
+
     </ul>
−
        <li>Purified the GG compatible phrAT PCR product from its agarose gel <em>[Maddie]</em></li>
+
−
        <li>Measured the OD730 of two cyanobacteria cultures <em>[Mark]</em></li>
+
−
              <ul>
+
−
                  <li>6.465 (#1), 4.660 (#4)</li>
+
−
                  <li>We decided to only continue measuring the the OD730 of growing cultures. Cultures #2 and #3 seem to have already plateaued</li>
+
−
              </ul>
+
−
        <li>Performed Golden Gate assembly to ligate Dsup to pDeg_pTrc, uvsE to pDeg_pTrc, Dsup to pDeg_PCPC, and uvsE to pDeg_PCPC <em>[Alex]</em> </li>
+
−
        <li>Ligated phrAT to lac+RBS+pSB1A2, RBS+blue chromoprotein to lac+RBS+Dsup+pSB1A2, RBS+blue chromoprotein to pSB1C3+lac+RBS+uvsE, and lac+RBS+uvsE to pSB4C5 <em>[Mark]</em></li>
+
−
         <li>Transformed DH5α E. coli cells with the ligated lac+RBS+phrAT+pSB1A2, lac+RBS+Dsup+RBS+blue chromoprotein+pSB1A2, lac+RBS+uvsE+RBS+blue chromoprotein+pSB1C3, and lac+RBS+uvsE+pSB4C5<em>[Mark, Zoe]</em>
+
−
        <li>Performed the UV irradiation test on DH5α cells with Dsup (with time intervals of 0, 15, 30, and 60 minutes) <em>[Micah, Mark]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Wednesday, August 9</u></strong></p>
+
     <p><strong><u>Thursday, August 9</u></strong></p>
−
     <p><em>Present: Zoe, Mark, Micah, Maddie, Collin, Alex</em></p>
+
     <p><em>Present: Cam, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Conducted collaboration video calls with Cardiff, Westminster, and University of Minnesota iGEM teams [Havisha, Kyle]</li>
−
         <li>Measured the OD730 of two cyanobacteria cultures <em>[Collin]</em></li>
+
        <li>Made competent <I>DH5α</I> cells [Cam]</li>
−
              <ul>
+
         <li>Performed PCR on yeast plasmid backbone [Havisha]</li>
−
                  <li>6.434 (#1), 4.676 (#4)</li>
+
         <li>Made and ran gel electrophoresis with PCR products [Havisha]</li>
−
              </ul>
+
     </ul>
−
        <li>Ligated phrAT to lac+RBS+pSB1A2, RBS+blue chromoprotein to lac+RBS+uvsE+pSB1A2, phrAC to lac+RBS+pSB1A2, and uvsE to lac+RBS+pSB1A2 <em>[Collin]</em></li>
+
−
         <li>Transformed lac+RBS+phrAT+pSB1A2, lac+RBS+uvsE+RBS+blue chromoprotein+pSB1A2, lac+RBS+phrAC+pSB1A2, and lac+RBS+uvsE+pSB1A2 into DH5α E. coli cells <em>[Collin, Zoe]</em></li>
+
−
         <li>Performed the UV irradiation test on DH5α cells with Dsup (with time intervals of 0, 30, 60, 90, and 120 minutes) <em>[Micah, Mark, Collin, Zoe, Maddie]</em></li>
+
−
       
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Thursday, August 10</u></strong></p>
+
     <p><strong><u>Friday, August 10</u></strong></p>
−
     <p><em>Present: Mark, Micah, Maddie, Collin, Alex</em></p>
+
     <p><em>Present: Elizabeth, Havisha</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Performed PCR on ribitol operon, ribitol transporter, and AvrSr35 [Havisha]</li>
−
         <li>Measured the OD730 of two cyanobacteria cultures <em>[Maddie]</em></li>
+
         <li>Made and ran gel electrophoresis with PCR products [Havisha]</li>
−
              <ul>
+
         <li>Transformed <I>DH5α</I> cells with mini prepped pSB1C3 plasmid with RFP [Elizabeth]</li>
−
                  <li>6.254 (#1), 4.047 (#4)</li>
+
         <li>Transformed <I>DH5α</I> cells with YFP Part BBa_I6031[Elizabeth]</li>
−
              </ul>
+
     </ul>
−
         <li>Performed gel electrophoresis on lac+RBS+uvsE+RBS+blue chromoprotein+pSB1C3, lac+RBS+Dsup+pSB4C5, Trc+Dsup, PCP+Dsup, Trc+uvsE, and PCP+uvsE <em>[Mark, Collin]</em>
+
−
         <li>Performed PCR to add golden gate ends onto phrAT <em>[Alex]</em></li>
+
−
         <li>Prepared overnight cultures of lac+RBS+pSB1C3, lac+RBS+uvsE+RBS+blue chromoprotein+pSB1A2 (3 cultures; #1, #2, #3), and lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3 <em>[Alex]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,560: Line 1,465:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Friday, August 11</u></strong></p>
+
     <p><strong><u>Saturday, August 11</u></strong></p>
−
     <p><em>Present: Micah, Maddie</em></p>
+
     <p><em>Present: Cam, Havisha</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Purified ribitol transporter and AvrSr35 from agarose gel [Cam, Havisha]</li>
−
        <li>Miniprepped the 3 cultures with lac+RBS+uvsE+RBS+blue chromoprotein+pSB1A2 <em>[Micah]</em></li>
+
        <li>Performed PCR on ribitol operon, ribitol transporter, and pRSII424 plasmid [Cam, Havisha]</li>
−
        <li>Digested lac+RBS+pSB1C3, uvsE+pSB1C3, phrAT+pSB1C3, phrAC+pSB1C3, Trc+Dsup, and PCP+uvsE <em>[Maddie]</em></li>
+
        <li>Made and ran gel electrophoresis with PCR products [Cam, Havisha]</li>
−
        <li>Performed gel electrophoresis on the digested lac+RBS+pSB1C3, uvsE+pSB1C3, phrAT+pSB1C3, phrAC+pSB1C3, Trc+Dsup, and PCP+uvsE <em>[Maddie]</em></li>
+
    </ul>
−
        <li>Tranformed plac Or2-62 (BBa_I12210) into DH5α E. Coli cells <em>[Micah]</em></li>
+
    <center>      
−
    </ol>
+
      <img src="https://static.igem.org/mediawiki/2018/7/77/T--WashU_StLouis--811notebook.jpeg" width= "20%" />  
−
    <p><strong> Outside Work / Discussion </strong><p>
+
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Cells transformed with pSB1C3 from 8/10</p>
 +
    </center>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Saturday, August 12</u></strong></p>
+
     <p><strong><u>Sunday, August 12</u></strong></p>
−
     <p><em>Present: Mark, Micah, Collin, Alex</em></p>
+
     <p><em>Present: Cam, Havisha</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Performed PCR on ribitol operon and ribitol transporter [Havisha]</li>
−
     
+
        <li>Made SOC Media [Cam]</li>
−
    </ol>
+
        <li>Purified pRSII424 plasmid from agarose gel [Cam, Havisha]</li>
−
    <p><strong> Outside Work / Discussion </strong><p>
+
        <li>Transformed <I>DH5α</I> cells with pSB1A3 plasmid [Cam, Havisha]</li>
−
    <ol>
+
        <li>Transformed <I>DH5α</I> cells with pSB1K3 plasmid [Cam, Havisha]</li>
−
         <li>Met up with the Missouri S&T iGEM Team in Rolla Missouri <em>[Alex, Collin, Mark, Micah]</em></li>
+
         <li>Transformed <I>DH5α</I> cells with YFP Part BBa_I6031 [Cam, Havisha]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,591: Line 1,498:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Sunday, August 13</u></strong></p>
+
     <p><strong><u>Monday, August 13</u></strong></p>
−
     <p><em>Present: Maddie, Alex</em></p>
+
     <p><em>Present: Cam, Havisha</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Began an overnight culture of cells transformed with pSB1A3 plasmid [Havisha]</li>
−
        <li>Gel purified lac+RBS+pSB1C3,lac+RBS+phrAC+pSB1C3, and lac+RBS+uvsE+pSB1C3 <em>[Maddie]</em></li>
+
        <li>Made LB agar + Kanamycin plates [Cam, Havisha]</li>
−
        <li>Ligated lac+RBS+uvsE and lac+RBS+phrAC <em>[Alex]</em></li>
+
        <li>Transformed <I>DH5α</I> cells with pSB1K3 plasmid [Cam, Havisha]</li>
−
        <li>Transformed lac+RBS+uvsE and lac+RBS+phrAC into DH5α E. Coli cells <em>[Alex]</em></li>
+
        <li>Transformed <I>DH5α</I> cells with YFP Part BBa_I6031 [Cam, Havisha]</li>
−
     </ol>
+
    </ul>
−
    <p><strong> Outside Work / Discussion </strong><p>
+
     <center>     
 +
      <img src="https://static.igem.org/mediawiki/2018/6/67/T--WashU_StLouis--8121notebook.jpeg" width= "20%" />  
 +
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Cells transformed with pSB1A3 from 8/12</p>
 +
    </center>
 +
    <center>     
 +
      <img src="https://static.igem.org/mediawiki/2018/b/bf/T--WashU_StLouis--8122notebook.jpeg" width= "20%" />  
 +
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Cells transformed with YFP from 8/12</p>
 +
    </center>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Monday, August 14</u></strong></p>
+
     <p><strong><u>Tuesday, August 14</u></strong></p>
−
     <p><em>Present: Mark, Micah, Maddie, Alex</em></p>
+
     <p><em>Present: Cam, Havisha</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Made LB + Amp Media [Cam, Havisha]</li>
−
         <li>Digested phrAT+pSB1C3 <em>[Maddie, Mark]</em></li>
+
        <li>Made LB + Kan Media [Cam, Havisha]</li>
−
         <li>Prepared liquid cultures of lac+RBS+uvsE+pSB1C3 and lac+RBS+phrAC+pSB1C3 (3 cultures of each; #20, #21, #22) <em>[Alex, Mark]</em></li>
+
         <li>Began an overnight culture of <I>DH5α</I> cells with pSB1K3 plasmid [Cam, Havisha]</li>
−
         <li>Performed the UV irradiation test on DH5α cells with Dsup (with time intervals of 0, 1, 2, 3, and 4 hours) <em>[Micah, Mark, Maddie]</em></li>
+
         <li>Began an overnight culture of <I>DH5α</I> cells with YFP Part BBa_I6031 [Cam, Havisha]</li>
−
        <li>Performed PCR to add Golden Braid ends to Dsup <em>[Alex]</em></li>
+
         <li>Began an overnight culture of <I>DH5α</I> cells with pSB1A3 plasmid [Cam, Havisha]</li>
−
     </ol>
+
    </ul>
−
     <p><strong> Outside Work / Discussion </strong><p>
+
    <center>      
 +
      <img src="https://static.igem.org/mediawiki/2018/1/10/T--WashU_StLouis--8141notebook.jpeg" width= "20%" />  
 +
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Cells transformed with pSB1K3 from 8/13</p>
 +
     </center>
 +
     <center>      
 +
      <img src="https://static.igem.org/mediawiki/2018/1/13/T--WashU_StLouis--8142notebook.jpeg" width= "20%" />  
 +
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Cells transformed with YFP from 8/13</p>
 +
    </center>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Tuesday, August 15</u></strong></p>
+
     <p><strong><u>Wednesday, August 15</u></strong></p>
−
     <p><em>Present: Mark, Micah, Maddie, Alex</em></p>
+
     <p><em>Present: Cam, Elizabeth, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Miniprepped pSB1K3 plasmid [Cam, Elizabeth, Havisha]</li>
−
         <li>Counted the colonies on the plates from the previous day's UV irradiation test <em>[Micah, Mark, Alex]</em></li>
+
         <li>Miniprepped YFP Part BBa_I6031 [Cam, Elizabeth, Havisha]</li>
−
         <li>Miniprepped the overnight cultures of lac+RBS+uvsE+pSB1C3 and lac+RBS+phrAC+pSB1C3 <em>[Maddie]</em></li>
+
         <li>Miniprepped pSB1A3 plasmid [Cam, Elizabeth, Havisha]</li>
−
         <li>Digested phrAT+pSB1C3, lac+RBS+phrAC+pSB1C3, and lac+RBS+uvsE <em>[Mark]</em></li>
+
         <li>Worked on arduino coding for device [Kyle]</li>
−
         <li>Performed gel electrophoresis on the digested phrAT+pSB1C3, lac+RBS+phrAC+pSB1C3, and lac+RBS+uvsE <em>[Mark]</em></li>
+
         <li>Transformed electrocompetent <I>DH5α</I> cells for practice [Cam, Elizabeth, Havisha, Kyle]</li>
−
         <li>Prepared liquid cultures of induced lac+RBS+Dsup+RBS+Blue chromoprotein+pSB1C3, non-induced lac+RBS+Dsup+RBS+Blue chromoprotein+pSB1C3, control DH5α E. Coli cells and lac+RBS+uvsE <em>[Alex]</em></li>
+
    </ul>
−
        <li>Performed PCR to add golden gate compatible ends to phrAT <em>[Maddie]</em></li>
+
    <center>      
−
        <li>Prepared LB+CM mini plates <em>[Mark]</em></li>
+
      <img src="https://static.igem.org/mediawiki/2018/e/e5/T--WashU_StLouis--815notebook.jpeg" width= "20%" />  
−
 
+
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Overnight cultures of YFP, pSB1A3 plasmid, and pSB1K3 plasmid from 8/14</p>
−
    </ol>
+
    </center>
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Wednesday, August 16</u></strong></p>
+
     <p><strong><u>Thursday, August 16</u></strong></p>
−
     <p><em>Present: Zoe, Mark, Micah, Maddie, Alex</em></p>
+
     <p><em>Present: Cam, Elizabeth, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Met with Dr. Shah from the Danforth Plant Science Center [Cam, Elizabeth, Havisha, Kyle]</li>
−
         <li>Performed gel purification on phrAT+pSB1C3 <em>[Maddie]</em></li>
+
         <li>Transformed <I>DH5α</I> cells for Day 1 of the interlab protocol [Cam, Elizabeth, Havisha, Kyle]</li>
−
         <li>Prepared 1200 mL of LB+Agar <em>[Maddie]</em></li>
+
         <li>Transformed electrocompetent <I>DH5α</I> cells for practice [Cam, Elizabeth, Havisha, Kyle]</li>
−
        <li>Performed the UV irradiation test on Dsup and the control <em>[Micah, Mark]</em></li>
+
     </ul>
−
        <li>Performed restriction digestion on lac+RBS+uvsE+pSB1C3, lac+RBS+phrAC+pSB1C3, and lac+RBS+pSB1C3 <em>[Maddie, Mark]</em></li>
+
−
         <li>Performed gel electrophoresis on the digested DNA <em>[Mark]</em></li>
+
−
        <li>Ligated lac+RBS+uvSE+pSB1C3 with lac+RBS+blue chromoprotein, lac+RBS+pSB1C3 with blue chromoprotein, and lac+RBS+pSB1C3 with phrAT <em>[Zoe, Alex]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Thursday, August 17</u></strong></p>
+
     <p><strong><u>Friday, August 17</u></strong></p>
−
     <p><em>Present: Zoe, Mark, Micah, Maddie, Alex</em></p>
+
     <p><em>Present: Cam, Elizabeth, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Attended the iGEM Midwest Meetup [Cam, Elizabeth]</li>
−
         <li>Digested lac+RBS+phrAC+pSB1C3 <em>[Maddie]</em></li>
+
         <li>Started overnight cultures for Day 2 of the interlab protocol [Kyle]</li>
−
        <li>Counted the colonies on the plates from the previous day's UV irradiation test <em>[Micah, Mark, Alex, Zoe]</em></li>
+
     </ul>
−
         <li>Performed a PCR gradient of uvsE for GoldenBraid <em>[Maddie]</em></li>
+
−
        <li>Prepared overnight cultures of Dsup+BCP, lac+RBS+phrAT+pSB1C3, and RBS+BCP+pSB1C3 <em>[Mark]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Friday, August 18</u></strong></p>
+
     <p><strong><u>Saturday, August 18</u></strong></p>
−
     <p><em>Present: Zoe, Mark, Micah, Maddie, Alex</em></p>
+
     <p><em>Present: Cam, Elizabeth, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Started overnight cultures for Day 2 of the interlab protocol [Elizabeth]</li>
−
         <li>Prepared glycerol stocks of DH5α E. coli cells that contained lac+RBS+phrAT+pSB1C3, lac+RBS+BCP+pSB1C3, and lac+RBS+Dsup+RBS+BCP+pSB1C3 <em>[Mark]</em></li>
+
         <li>Transformed <I>DH5α</I> cells with positive control, negative control, and test device two for Day 1 of the interlab protocol [Cam, Elizabeth, Kyle]</li>
−
         <li>Miniprepped lac+RBS+phrAT+pSB1C3 <em>[Zoe]</em></li>
+
     </ul>
−
        <li>Performed a UV irradiation test for Dsup and the Blue chromoprotein control <em>[Micah, Mark]</em></li>
+
−
        <li>Performed PCR for uvsE <em>[Maddie, Zoe]</em></li>
+
−
        <li>Digested lac+RBS+phrAT+pSB1C3 <em>[Alex]</em></li>
+
−
        <li>Ran a gel electrophoresis for the digested lac+RBS+phrAT+pSB1C3 <em>[Mark]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Saturday, August 19</u></strong></p>
+
     <p><strong><u>Sunday, August 19</u></strong></p>
−
     <p><em>Present: Zoe, Maddie, Mark</em></p>
+
     <p><em>Present: Cam, Elizabeth</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Made overnight cultures of positive control, negative control, and test device two for Day 2 of the interlab protocol [Cam]</li>
−
         <li>Counted colonies on the plates from the UV irradiation test on Dsup <em>[Zoe, Maddie, Mark]</em></li>
+
         <li>Performed part three of the interlab calibration protocol [Elizabeth]</li>
−
    </ol>
+
        <li>Made LB agar and chloramphenicol plates [Elizabeth]</li>
−
     <p><strong> Outside Work / Discussion </strong><p>
+
     </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,707: Line 1,612:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Sunday, August 20</u></strong></p>
+
     <p><strong><u>Monday, August 20</u></strong></p>
−
     <p><em>Present: Micah</em></p>
+
     <p><em>Present: Cam, Elizabeth, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Finalized sequencing primers [Elizabeth]</li>
−
         <li>Prepared overnight cultures of DH5α E. coli cells with lac+RBS+blue chromoprotein+pSB1C3, lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3, and lac+RBS+uvsE+RBS+blue chromoprotein+pSB1C3 (#1) <em>[Micah]</em></li>
+
         <li>Transformed <I>DH5α</I> cells with positive control, test device one, test device two, and test device three for Day 1 of the interlab protocol [Cam, Elizabeth, Kyle]</li>
−
    </ol>
+
        <li>Made LB agar and chloramphenicol plates [Cam, Elizabeth]</li>
−
    <p><strong> Outside Work / Discussion </strong><p>
+
        <li>Transformed <I>DH5α</I> cells with YFP [Kyle]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,720: Line 1,626:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Monday, August 21</u></strong></p>
+
     <p><strong><u>Tuesday, August 21</u></strong></p>
−
     <p><em>Present:Mark, Micah</em></p>
+
     <p><em>Present: Cam, Elizabeth, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Transformed <I>DH5α</I> cells with Gal1, Tef2, and RFP + terminator [Cam, Elizabeth, Kyle]</li>
−
         <li>Prepared overnight cultures of DH5α E. coli cells with pSB1C3+lac+RBS+phrAC+RBS+blue chromoprotein (2 cultures, #1 and #2), pSB1C3+lac+RBS+blue chromoprotein, and pSB1C3+lac+RBS+Dsup+RBS+blue chromoprotein <em>[Mark]</em></li>
+
     </ul>
−
        <li>Miniprepped DH5α E. coli cell cultures with lac+RBS+blue chromoprotein+pSB1C3, lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3, and lac+RBS+uvsE+RBS+blue chromoprotein+pSB1C3 (#1) <em>[Micah]</em></li>
+
−
    </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
−
    <ol>
+
−
        <li>Watched the Great American Eclipse of 2017 <em>[Micah, Mark, Zoe, Maddie, Alex, Collin]</em></li>
+
−
     </ol>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,737: Line 1,637:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Tuesday, August 22</u></strong></p>
+
     <p><strong><u>Wednesday, August 22</u></strong></p>
−
     <p><em>Present: Mark, Zoe, Micah, Alex, Maddie</em></p>
+
     <p><em>Present: Cam, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Autoclaved water and culture tubes [Cam, Kyle]</li>
−
         <li>Miniprepped lac+RBS+phrAC+RBS+BCP+pSB1C3 <em>[Zoe]</em></li>
+
         <li>Made overnight cultures of Gal1, Tef2, and RFP + terminator [Cam]</li>
−
         <li>Gel purified lac+RBS+phrAT+pSB1C3 <em>[Maddie]</em></li>
+
     </ul>
−
        <li>Prepared LB+CM plates <em>[Mark]</em></li>
+
−
        <li>Performed the UV irradiation test on uvsE, phrAC, and the blue chromoprotein control <em>[Micah, Mark]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,753: Line 1,649:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Wednesday, August 23</u></strong></p>
+
     <p><strong><u>Thursday, August 23</u></strong></p>
−
     <p><em>Present: Zoe, Maddie, Mark, Alex, Micah</em></p>
+
     <p><em>Present: Cam</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Made overnight cultures of for Day 2 of the interlab protocol [Cam]</li>
−
         <li>Counted the colonies on the plates from the previous day's UV irradiation test <em>[Micah, Alex, Mark, Zoe, Maddie]</em></li>
+
     </ul>
−
        <li>Streaked lac+RBS+uvsE+RBS+BCP+pSB1C3 and lac+RBS+phrAC+RBS+BCP+pSB1C3 onto LB+CM plates <em>[Micah]</em></li>
+
−
        <li>Ligated lac+RBS+phrAT+pSB1C3  with RBS+BCP <em>[Micah]</em></li>
+
−
        <li>Transformed DH5α E. coli cells with lac+RBS+phrAT+RBS+BCP+pSB1C3 <em>[Mark]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,769: Line 1,660:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Thursday, August 24</u></strong></p>
+
     <p><strong><u>Friday, August 24</u></strong></p>
−
     <p><em>Present:Micah</em></p>
+
     <p><em>Present: Elizabeth, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Performed hour zero preparations for Interlab Day 3 [Havisha]</li>
−
         <li>Prepared overnight cultures of DH5α E. coli cells with lac+RBS+phrAT+RBS+BCP+pSB1C3 <em>{Micah]</em></li>
+
        <li>Performed hour six for Interlab Day 3 [Elizabeth]</li>
−
    </ol>
+
        <li>Set up digestion reactions for Sr35 part 1, Sr35 part 2, GAL1, and TEF1 [Elizabeth]</li>
−
    <p><strong> Outside Work / Discussion </strong><p>
+
         <li>Ran gel with Sr35 part 1, Sr35 part 2, GAL1, and TEF1 [Elizabeth, Havisha]</li>
 +
        <li>Performed PCR for AvrSr35 [Havisha]</li>
 +
        <li>Autoclaved tips [Kyle]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,782: Line 1,676:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Friday, August 25</u></strong></p>
+
     <p><strong><u>Saturday, August 25</u></strong></p>
−
     <p><em>Present: Zoe</em></p>
+
     <p><em>Present: Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Purified GAL1 and TEF1 from gel [Havisha]</li>
−
         <li>Miniprepped lac+RBS+phrAT+RBS+BCP+pSB1C3 <em>[Zoe]</em></li>
+
         <li>Performed PCR for AvrSr35 [Kyle]</li>
−
    </ol>
+
        <li>Ran gel with AvrSr35 PCR product to confirm fragment size [Havisha]</li>
−
     <p><strong> Outside Work / Discussion </strong><p>
+
     </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,795: Line 1,689:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Saturday, August 26</u></strong></p>
+
     <p><strong><u>Sunday, August 26</u></strong></p>
−
    <p><em></em></p>
+
 
     <p>No work done today!</p>
 
     <p>No work done today!</p>
 
     </div>
 
     </div>
Line 1,804: Line 1,697:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Sunday, August 27</u></strong></p>
+
     <p><strong><u>Monday, August 27</u></strong></p>
−
     <p><em></em></p>
+
     <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Met with Dr. Brennan to discuss project progress, logistics, and possible troubleshooting [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,813: Line 1,708:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Monday, August 28</u></strong></p>
+
     <p><strong><u>Tuesday, August 28</u></strong></p>
−
     <p><em></em></p>
+
     <p><em>Present: Elizabeth, Havisha, Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Performed PCR for pRSII424 plasmid [Elizabeth]
 +
        <li>Set up digestion reactions for ribitol operon and ribitol transporter [Elizabeth, Kyle]
 +
        <li>Set up digestion reactions for RFP + terminator and promoter + RBS [Elizabeth, Havisha]
 +
        <li>Made and ran gel with ribitol operon and ribitol transporter [Elizabeth, Havisha]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,822: Line 1,722:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Tuesday, August 29</u></strong></p>
+
     <p><strong><u>Wednesday, August 29</u></strong></p>
−
     <p><em></em></p>
+
     <p><em>Present: Cam, Havisha</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Purified ribitol transporter from gel [Havisha]</li>
 +
        <li>Made and ran gel to confirm pRSII424 plasmid [Cam, Havisha]</li>
 +
        <li>Purified digestion reactions for RFP + terminator [Cam, Havisha]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,831: Line 1,735:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Wednesday, August 30</u></strong></p>
+
     <p><strong><u>Thursday, August 30</u></strong></p>
−
     <p><em></em></p>
+
     <p><em>Present: Cam, Elizabeth, Havisha, Kyle</em></p>
−
    <p>No work done today!</p>
+
    <ul>
 +
        <li>Made and ran gel to confirm pRSII424 plasmid and AvrSr35 [Cam, Elizabeth, Havisha]</li>
 +
        <li>Set up ligation reaction for ribitol transporter and RFP + terminator [Cam, Elizabeth, Havisha]</li>
 +
        <li>Transformed <I>DH5α</I> cells with ligation products [Cam, Elizabeth, Kyle]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,840: Line 1,748:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Thursday, August 31</u></strong></p>
+
     <p><strong><u>Friday, August 31</u></strong></p>
−
     <p><em>Present: Collin</em></p>
+
     <p><em>Present: Elizabeth</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Checked transformation plates [Elizabeth]</li>
−
        <li>Prepared overnight cultures from glycerol stock of lac+RBS+BCP+pSB1C3 <em>[Collin]</em></li>
+
     </ul>
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,853: Line 1,759:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Friday, September 1</u></strong></p>
+
     <p><strong><u>Saturday, September 1</u></strong></p>
 
     <p><em></em></p>
 
     <p><em></em></p>
 
     <p>No work done today!</p>
 
     <p>No work done today!</p>
Line 1,862: Line 1,768:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Saturday, September 2</u></strong></p>
+
     <p><strong><u>Sunday, September 2</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Elizabeth</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Checked transformation plates [Elizabeth]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,871: Line 1,779:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Sunday, September 3</u></strong></p>
+
     <p><strong><u>Monday, September 3</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Elizabeth</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Made PCR Mix [Elizabeth]</li>
 +
        <li>Performed PCR for Sr35 part 1, AvrSr35, RFP + terminator, ribitol operon, promoter + RBS, and ribiol transporter [Elizabeth]</li>
 +
        <li>Made and ran gel with Sr35 part 1, AvrSr35, RFP + terminator, ribitol operon, promoter + RBS, and ribiol transporter [Elizabeth]</li>
 +
        <li>Set up digestion reaction for Sr35 part 1, AvrSr35, RFP + terminator, ribitol operon, promoter + RBS, and ribiol transporter [Elizabeth]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,880: Line 1,793:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Monday, September 4</u></strong></p>
+
     <p><strong><u>Tuesday, September 4</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha</em></p>
−
    <p>No work done today!</p>
+
    <ul>
 +
        <li>Performed PCR for ribitol operon and ribitol transporter [Elizabeth]</li>
 +
        <li>Set up digestion reactions for RFP + terminator, ribitol operon, promoter + RBS, and ribiol transporter [Elizabeth]</li>
 +
        <li>Made and ran gel with RFP + terminator, ribitol operon, promoter + RBS, and ribiol transporter [Cam, Elizabeth]</li>
 +
        <li>Made and ran gel with AvrSr35, Sr35 part 1, RFP + terminator, yeast plasmid, and promoter + RBS [Elizabeth]</li>
 +
        <li>Purified AvrSr35, Sr35 part 1, yeast plasmid, and promoter + RBS from gel [Havisha]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,889: Line 1,808:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Tuesday, September 5</u></strong></p>
+
     <p><strong><u>Wednesday, September 5</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Elizabeth, Havisha</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Set up digestion reactions for Tef1 and RFP + terminator [Havisha]</li>
 +
        <li>Made and ran gel with Tef1 and RFP + terminator [Havisha]</li>
 +
        <li>Purified Tef1 and RFP + terminator from gel [Elizabeth, Havisha]</li>
 +
        <li>Set up digestion reaction for Tef1 [Elizabeth]</li>
 +
        <li>Made and ran gel with Tef1 and cut out bands [Elizabeth, Havisha]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Wednesday, September 6</u></strong></p>
+
     <p><strong><u>Thursday, September 6</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha, Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Purified Tef 1 from gel [Elizabeth]
 +
        <li>Set up digestion reaction for AvrSr35 and Sr35 part 1 [Elizabeth]</li>
 +
        <li>Made and ran gel with AvrSr35 and Sr35 part 1 [Havisha, Kyle]</li>
 +
        <li>Made LB agar and ampicillin plates [Havisha, Kyle]</li>
 +
        <li>Purified AvrSr35 and Sr35 part 1 from gel [Elizabeth]</li>
 +
        <li>Set up ligation reaction for AvrSr35 + Gal1 and Sr35 + Gal1 [Havisha]</li>
 +
        <li>Transformed <I>DH5α</I> cells with ligation products [Cam, Havisha]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,907: Line 1,840:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Thursday, September 7</u></strong></p>
+
     <p><strong><u>Friday, September 7</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present:Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Met to discuss goals for the week and progress [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
 +
        <li>Checked plates [Havisha]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,916: Line 1,852:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Friday, September 8</u></strong></p>
+
     <p><strong><u>Saturday, September 8</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Elizabeth</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Performed PCR on ribitol transporter, AvrSr35, pRS424 plasmid, Sr35 [Elizabeth]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,925: Line 1,863:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Saturday, September 9</u></strong></p>
+
     <p><strong><u>Sunday, September 9</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Diva, Elizabeth, Havisha</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Set up digestion reactions for pRS424 plasmid and pSB1K3 plasmid [Elizabeth, Havisha]
 +
        <li>Made and ran gel for pRS424 plasmid [Elizabeth, Havisha]</li>
 +
        <li>Purified pRS424 plasmid from gel [Elizabeth, Havisha]</li>
 +
        <li>Made and ran gel for pSB1K3 plasmid [Havisha]</li>
 +
        <li>pSB1K3 plasmid’s RFP insert  from gel [Havisha]</li>
 +
        <li>Set up ligation reaction between pRS424 plasmid and RFP insert [Cam]</li>
 +
        <li>Transformed <I>DH5α</I> cells with ligation products [Cam]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,934: Line 1,880:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Sunday, September 10</u></strong></p>
+
     <p><strong><u>Monday, September 10</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Met to discuss goals for the week and progress [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
 +
        <li>Checked plates [Elizabeth]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,943: Line 1,892:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Monday, September 11</u></strong></p>
+
     <p><strong><u>Tuesday, September 11</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha, Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Met to edit abstract [Cam, Elizabeth, Havisha, Kyle]</li>
 +
        <li>Made and ran gel for ribitol transporter [Elizabeth]</li>
 +
        <li>Set up digestion for AvrSr35 [Elizabeth]</li>
 +
        <li>PCR purified digestion of AvrSr35 [Elizabeth, Havisha]</li>
 +
        <li>Set up ligation reaction between AvrSr35 and Gal1 [Elizabeth, Havisha]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,952: Line 1,907:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Tuesday, September 12</u></strong></p>
+
     <p><strong><u>Wednesday, September 12</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Elizabeth, Havisha, Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Set up digestion for Sr35 part 1 [Elizabeth]</li>
 +
        <li>PCR purified digestion of Sr35 part 1 [Elizabeth, Havisha]</li>
 +
        <li>Set up ligation reaction between Sr35 part 1 and Gal1 [Havisha]</li>
 +
        <li>Transformed <I>DH5α</I> cells with ligation products [Havisha, Kyle]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,961: Line 1,921:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Wednesday, September 13</u></strong></p>
+
     <p><strong><u>Thursday, September 13</u></strong></p>
−
     <p><em></em></p>
+
     <p><em>Present: Elizabeth</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Checked plates [Elizabeth]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,970: Line 1,932:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Thursday, September 14</u></strong></p>
+
     <p><strong><u>Friday, September 14</u></strong></p>
−
    <p><em>Present:Mark</em></p>
+
  <p><em>Present: Elizabeth, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Performed PCR on pRS424 plasmid [Elizabeth]</li>
−
        <li>Transformed Lac+RBS+BCP+pSB1C3 into E.coli cells <em>[Mark]</em></li>
+
        <li>Set up digestion for AvrSr35, pRS424 plasmid, ribitol transporter, and Sr35 part 1 [Elizabeth]</li>
−
    </ol>
+
        <li>PCR purified AvrSr35, pRS424 plasmid, ribitol transporter, and Sr35 [Havisha]</li>
−
    <p><strong> Outside Work / Discussion </strong><p>
+
        <li>Plated cells transformed with pSB1C3 [Kyle]</li>
 +
        <li>Set up digestion for promoter + RBS, ribitol transporter, and RFP + terminator [Elizabeth]</li>
 +
        <li>Organized parts and enzymes inventory [Elizabeth, Kyle]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,983: Line 1,948:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Friday, September 15</u></strong></p>
+
     <p><strong><u>Saturday, September 15</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Elizabeth, Havisha, Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Checked plates [Havisha]</li>
 +
        <li>PCR purified promoter + RBS, ribitol transporter, and RFP + terminator [Havisha]</li>
 +
        <li>Began overnight cultures of pSB1C3 [Kyle]</li>
 +
        <li>PCR purified AvrSr35, Sr35 part 1, and pRS424 plasmid [Havisha]</li>
 +
        <li>Set up digestion reactions for Sr35 part 1, pRS424 plasmid, pSB1C3, and promoter + RBS [Havisha]</li>
 +
        <li>Set up ligation reaction between Sr35 part 1 and Gal1 and pRS424 plasmid and RFP insert from pSB1C3 [Havisha]</li>
 +
        <li>Performed PCR on ribitol operon and ribitol transporter [Elizabeth]</li>
 +
    </ul>
 +
    <center>
 +
      <img src="https://static.igem.org/mediawiki/2018/d/d6/T--WashU_StLouis--915notebook.jpeg" width= "300" />
 +
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Plated cells transformed with pSB1C3 from 9/14</p>
 +
    </center>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 1,992: Line 1,969:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Saturday, September 16</u></strong></p>
+
     <p><strong><u>Monday, September 16</u></strong></p>
−
    <p><em>Present:Maddie</em></p>
+
  <p><em>Present: Elizabeth, Havisha</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Miniprepped pSB1C3 [Elizabeth]</li>
−
        <li>Started a cyanobacteria culture <em>[Maddie]</em></li>
+
        <li>PCR purified ribitol transporter and AvrSr35 [Havisha]</li>
−
        <li>Performed GoldenBraid PCR for uvsE, phrAC, phrAT, and Dsup <em>[Maddie]</em></li>
+
        <li>Performed PCR on AvrSr35 [Elizabeth, Havisha]</li>
−
     </ol>
+
        <li>Made and ran gel with ribitol operon [Havisha]</li>
−
     <p><strong> Outside Work / Discussion </strong><p>
+
        <li>Set up digestion reactions for ribitol transporter and RFP + terminator [Havisha]</li>
 +
        <li>Set up ligation reaction between Sr35 part 1 and Gal1, pRS424 plasmid and RFP insert from pSB1C3, ribitol transporter and promoter + RBS, and RFP + terminator</li>  
 +
        <li>Transformed <I>DH5α</I> cells with ligation products and pSB1C3</li>
 +
     </ul>
 +
     <center>
 +
      <img src="https://static.igem.org/mediawiki/2018/6/62/T--WashU_StLouis--916notebook.jpeg" width= "300" />
 +
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Overnight cultures of pSB1C3 from 9/15</p>
 +
    </center>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,006: Line 1,990:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Sunday, September 17</u></strong></p>
+
     <p><strong><u>Monday, September 17</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha, Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Met to discuss project progress and timeline [Cam, Elizabeth, Havisha, Kyle]</li>
 +
        <li>Made and ran a gel with ribitol operon PCR product [Havisha, Kyle]</li>
 +
        <li>Began overnight cultures of DH5α transformed with Sr35 part 1 and Gal1 plasmid, pRS424 plasmid with RFP insert, and ribitol transporter and promoter + RBS plasmid [Havisha, Kyle]</li>
 +
        <li>Set up digestion reaction for AvrSr35 [Havisha]</li>
 +
        <li>Set up ligation reaction between AvrSr35 and Gal1 [Havisha]</li>
 +
        <li>Transformed <I>DH5α</I> with ligation products [Cam]</li>
 +
        <li>Made chloramphenicol plates [Cam]</li>
 +
    </ul>
 +
    <center>
 +
      <img src="https://static.igem.org/mediawiki/2018/6/60/T--WashU_StLouis--9171notebook.jpeg" width= "300" />
 +
      <img src="https://static.igem.org/mediawiki/2018/a/a6/T--WashU_StLouis--9172notebook.jpeg" width= "300" />
 +
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Plated cells transformed with ligation products from 9/16</p>
 +
    </center>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,015: Line 2,012:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Monday, September 18</u></strong></p>
+
     <p><strong><u>Tuesday, September 18</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Miniprepped Sr35 part 1 and Gal1 plasmid, pRS424 plasmid with RFP insert, and ribitol transporter and promoter + RBS plasmid [Cam, Elizabeth, Havisha]</li>
 +
        <li>Made and ran gels with miniprepped plasmids [Elizabeth, Havisha, Kyle, Diva]</li>
 +
        <li>Conducted a collaboration call with Cardiff regarding hardware, wiki, and parts collaboration [Havisha, Kyle]</li>
 +
    </ul>
 +
    <center>
 +
      <img src="https://static.igem.org/mediawiki/2018/b/ba/T--WashU_StLouis--918notebook.jpeg" width= "100" />
 +
      <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Gel with ligation products</p>
 +
    </center>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,024: Line 2,029:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Tuesday, September 19</u></strong></p>
+
     <p><strong><u>Wednesday, September 19</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Made and ran a gel with ribitol transporter and promoter + RBS plasmid [Cam, Elizabeth, Havisha]</li>
 +
        <li>Set up digestion reaction for Sr35 part 2, Gal1, ribitol transporter, Sr35 part 1 + Gal1 plasmid, and promoter+RBS [Elizabeth]</li>
 +
        <li>Set up ligation reaction between AvrSr35 and Gal1 promoter, Sr35 part 2 and Sr35 part 1 + Gal1 plasmid [Cam, Havisha]</li>
 +
        <li>Transformed <I>DH5α</I> with ligation products and RFP + terminator [Cam]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,033: Line 2,043:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Wednesday, September 20</u></strong></p>
+
     <p><strong><u>Thursday, September 20</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Elizabeth, Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Began overnight cultures of DH5α transformed with AvrSr35 + Gal 1 plasmid [Kyle]<li>
 +
        <li>Set up digestion reaction for Sr35 part 2 [Elizabeth]<li>
 +
        <li>Set up ligation reaction between Sr35 part 2 and Sr35 part 1 + Gal1 plasmid [Elizabeth]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,042: Line 2,056:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Thursday, September 21</u></strong></p>
+
     <p><strong><u>Friday, September 21</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Miniprepped AvrSr35 plasmid [Havisha]
 +
        <li>Made and ran gel to screen AvrSr35 plasmid and pRS424 plasmid with RFP insert [Elizabeth, Havisha, Cam]
 +
        <li>Performed PCR on ribitol operon [Elizabeth]
 +
        <li>Set up digestion reactions for AvrSr35 and Tef1 [Cam, Elizabeth]
 +
        <li>Set up ligation reaction between AvrSr35 and Tef1 [Cam, Elizabeth]
 +
        <li>Transformed <I>DH5α</I> with AvrSr35 + Tef1 plasmid [Cam, Elizabeth]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,051: Line 2,072:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Friday, September 22</u></strong></p>
+
     <p><strong><u>Saturday, September 22</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Began overnight cultures of Sr35 full construct and AvrSr35 + Tef1 plasmids [Kyle]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,060: Line 2,083:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Saturday, September 23</u></strong></p>
+
     <p><strong><u>Sunday, September 23</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Diva, Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Miniprepped Sr35 full construct and AvrSr35 + Tef1 plasmids [Diva, Kyle]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,069: Line 2,094:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Sunday, September 24</u></strong></p>
+
     <p><strong><u>Monday, September 24</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Met to discuss project progress and timeline [Diva, Elizabeth, Havisha, Kyle]</li>
 +
        <li>Performed PCR on ribitol operon [Elizabeth]</li>
 +
        <li>Made and ran gel to screen Sr35 full construct and AvrSr35 + Tef1 plasmids [Cam, Elizabeth, Kyle]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,078: Line 2,107:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Monday, September 25</u></strong></p>
+
     <p><strong><u>Tuesday, September 25</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Made YPD media [Cam, Elizabeth]</li>
 +
        <li>Performed PCR on ribitol transporter [Elizabeth]</li>
 +
        <li>PCR purified ribitol transporter [Elizabeth]</li>
 +
        <li>Set up digestion reactions for pRS424 plasmid, Gal1, Sr35 full construct, and AvrSr35 full construct [Elizabeth]</li>
 +
        <li>Began overnight culture of promoter + RBS [Elizabeth]</li>
 +
        <li>Set up ligation reactions between pRS424 plasmid and RFP insert, pRS424 plasmid and Sr35 construct, pRS424 plasmid and Gal1 promoter, and pRS424 plasmid and AvrSr35 full construct [Elizabeth, Havisha]</li>
 +
        <li>Transformed <I>DH5α</I> with ligation products [Havisha]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,087: Line 2,124:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Tuesday, September 26</u></strong></p>
+
     <p><strong><u>Wednesday, September 26</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Havisha</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Miniprepped promoter + RBS [Cam, Havisha]</li>
 +
        <li>Set up digestion reaction for promoter + RBS [Havisha]</li>
 +
        <li>PCR purified promoter + RBS [Cam, Havisha]</li>
 +
        <li>Set up ligation reaction between Sr35 full construct and pSB1C3 [Cam, Havisha]</li>
 +
        <li>Set up ligation reaction between promoter + RBS and ribitol transporter [Cam]</li>
 +
        <li>Transformed DH5α with Sr35 full construct + pSB1c3, promoter+RBS + ribitol transporter plasmid, and unknowns 1, 2, and 3 [Cam]</li>
 +
        <li>Performed PCR on ribitol operon [Cam]</li>
 +
        <li>Made YPD media [Cam]</li>
 +
        <li>Began overnight cultures of pRS424 plasmid + AvrSr35 full construct, pRS424 plasmid + RFP, pRS424 plasmid + Gal1, and pRS424 plasmid + Sr35 full construct [Cam]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,096: Line 2,143:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Wednesday, September 27</u></strong></p>
+
     <p><strong><u>Thursday, September 27</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Havisha, Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Miniprepped pRS424 plasmid + AvrSr35 full construct, pRS424 plasmid + RFP, pRS424 plasmid + Gal1, and pRS424 plasmid + Sr35 full construct  [Havisha]</li>
 +
        <li>Began overnight cultures of promoter + RBS + ribitol transporter plasmid and Unknowns 1, 2, and 3 [Kyle]</li>
 +
        <li>Made YPD agar media [Kyle]</li>
 +
        <li>Made LB media [Kyle]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,105: Line 2,157:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Thursday, September 28</u></strong></p>
+
     <p><strong><u>Friday, September 28</u></strong></p>
−
    <p><em>Present: Collin</em></p>
+
  <p><em>Present: Cam, Elizabeth</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Set up digestion and ligation reactions for AvrSr35 and pSB1C3 plasmid [Cam, Elizabeth]</li>
−
        <li>Refreshed Cyanobacteria culture <em>[Collin]</em></li>
+
        <li>Set up ligation reaction between Sr35 full construct and pSB1C3 [Cam, Elizabeth]</li>
−
    </ol>
+
        <li>Transformed <I>DH5α</I> with Sr35+pSB1C3 and AvrSr35+pSB1C3</li>
−
    <p><strong> Outside Work / Discussion </strong><p>
+
        <li>Began overnight cultures of promoter + RBS + ribitol transporter plasmid and Unknowns 1, 2, and 3 [Cam, Elizabeth]</li>
 +
        <li>Plated <I>S. cerevisiae EBY100</i> [Cam, Elizabeth]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,118: Line 2,172:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Friday, September 29</u></strong></p>
+
     <p><strong><u>Saturday, September 29</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Elizabeth</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Miniprepped promoter + RBS + ribitol transporter plasmid and Unknowns 1, 2, and 3 [Cam]</li>
 +
        <li>Began overnight cultures of Sr35+pSB1C3 and AvrSr35+pSB1C3 [Elizabeth]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,127: Line 2,184:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Saturday, September 30</u></strong></p>
+
     <p><strong><u>Sunday, September 30</u></strong></p>
−
    <p><em>Present:Collin, Micah, Mark</em></p>
+
  <p><em>Present: Cam, Elizabeth</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Miniprepped Sr35+pSB1C3 and AvrSr35+pSB1C3 [Cam]</li>
−
        <li>Performed PCR on Dsup, uvsE, phrAC, and phrAT for the GoldenBraid assembly <em>[Collin]</em></li>
+
        <li>Made and ran gel to screen Sr35+pSB1C3 and AvrSr35+pSB1C3 [Elizabeth]</li>
−
        <li>Performed the UV irradiaton test for uvsE, phrAC, and the control <em>[Micah, Mark, Collin]</em></li>
+
     </ul>
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,141: Line 2,196:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Sunday, October 1</u></strong></p>
+
     <p><strong><u>Monday, October 1</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha, Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Met to discuss project progress and timeline [Cam, Kyle]</li>
 +
        <li>Made and ran gel to screen ribitol operon PCR, pSB1C3 plasmid + Sr35 full construct, promoter + RBS + ribitol transporter plasmid, and pRS424 plasmid + RFP [Elizabeth, Havisha]</li>
 +
        <li>Made and ran gel to screen pSB1C3 plasmid + AvrSr35 full construct, RFP + term plasmid, and pRS424 plasmid + AvrSr35 full construct [Havisha]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,150: Line 2,209:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Monday, October 2</u></strong></p>
+
     <p><strong><u>Tuesday, October 2</u></strong></p>
−
    <p><em>Present: Collin</em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Made and ran gels to screen pRS424 plasmid + RFP, pRS424 plasmid + AvrSr35 full construct, pSB1C3 plasmid + Sr35 full construct, and pSB1C3 plasmid + AvrSr35 full construct [Elizabeth, Havisha]</li>
−
        <li>Measured the ODs of the Cyanobacteria culture <em>[Collin]</em></li>
+
        <li>Set up digestion reactions for Tef1, pRS424 plasmid, RFP, pSB1C3 plasmid + AvrSr35, Sr35 full construct, Improved part, and Gal1 [Elizabeth]
−
            <ul>
+
        <li>PCR purified digestions [Elizabeth]</li>
−
                <li> 3.413 (#1), 0.1370 (#2)</li>
+
        <li>Set up ligation reactions between Tef1 and pRS424 plasmid, RFP and pRS424 plasmid, pSB1C3 plasmid + AvrSr35 and Sr35 full construct, and Improved part and Gal1 [Elizabeth, Havisha]</li>
−
            </ul>
+
        <li>Transformed <I>DH5α</I> with ligation products [Cam]</li>
−
    </ol>
+
        <li>Made LB agar + CM plates [Cam, Havisha]</li>
−
    <p><strong> Outside Work / Discussion </strong><p>
+
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,166: Line 2,225:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Tuesday, October 3</u></strong></p>
+
     <p><strong><u>Wednesday, October 3</u></strong></p>
−
    <p><em>Present:Maddie</em></p>
+
  <p><em>Present: Havisha</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Made overnight cultures of <I>DH5α</I> transformed with Tef1 and pRS424 plasmid, RFP and pRS424 plasmid, pSB1C3 plasmid + AvrSr35 and Sr35 full construct, and Improved part and Gal1 [Havisha]</li>
−
         <li> Measured the OD of the cyanobacteria culture <em>[Maddie]</em></li>
+
    </ul>
−
              <ul>
+
−
                  <li> 0.1452 (#2)</li>
+
−
              </ul>
+
−
    </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,182: Line 2,236:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Wednesday, October 4</u></strong></p>
+
     <p><strong><u>Thursday, October 4</u></strong></p>
−
    <p><em>Present:Maddie</em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Miniprepped Tef1 and pRS424 plasmid, RFP and pRS424 plasmid, pSB1C3 plasmid + AvrSr35 and Sr35 full construct, and Improved part and Gal1 [Cam, Elizabeth, Havisha]</li>
−
         <li> Measured the OD of the cyanobacteria culture <em>[Maddie]</em></li>
+
        <li>Made and ran gel to screen Tef1 and pRS424 plasmid, RFP and pRS424 plasmid, pSB1C3 plasmid + AvrSr35 and Sr35 full construct, and Improved part and Gal1 [Elizabeth, Havisha]</li>
−
              <ul>
+
    </ul>
−
                  <li> 0.3725 (#2)</li>
+
−
              </ul>     
+
−
    </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,198: Line 2,248:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Thursday, October 5</u></strong></p>
+
     <p><strong><u>Friday, October 5</u></strong></p>
−
    <p><em>Present:Maddie</em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Made and ran a gel to screen pRS424 plasmid + RFP [Cam, Elizabeth, Havisha]</li>
−
         <li> Measured the OD of the cyanobacteria culture <em>[Maddie]</em></li>
+
         <li>Set up digestion reactions for Gal1+Improved Part, Tef1, pSB1C3, Sr35 full construct, and AvrSr35 full construct [Cam, Elizabeth, Havisha]</li>
−
              <ul>
+
        <li>PCR purified Gal1+Improved Part, Tef1, pSB1C3, Sr35 full construct, and AvrSr35 full construct [Havisha]</li>
−
                  <li> 0.6746 (#2)</li>
+
        <li>Set up ligation reactions between Gal1+Improved Part and Tef1, and Sr35 full construct and AvrSr35 full construct [Elizabeth]</li>
−
              </ul>            
+
     </ul>
−
    </ol>
+
−
     <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,214: Line 2,262:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Friday, October 6</u></strong></p>
+
     <p><strong><u>Saturday, October 6</u></strong></p>
−
    <p><em>Present:Mark</em></p>
+
  <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Transformed <I>DH5α</I> with Gal1+Improved Part+Tef1 plasmid, and Sr35 full construct+AvrSr35 full construct plasmid [Havisha, Kyle]</li>
−
         <li>Prepared LB Media <em>[Mark]</em></li>
+
         <li>Set up digestion reactions for promoter+RBS, ribitol transporter, RFP+terminator, ribitol operon, and Gal1+Improved Part [Cam, Elizabeth, Havisha]</li>
−
    </ol>
+
        <li>PCR purified promoter+RBS, ribitol transporter, RFP+term, ribitol operon, and Gal1+Improved Part [Elizabeth, Havisha]</li>
−
    <p><strong> Outside Work / Discussion </strong><p>
+
        <li>Ligate promoter+RBS and ribitol transporter, ribitol operon and RFP+terminator, and Gal1+Improved Part and pSB1C3 [Cam,  Elizabeth]</li>
−
     </div>
+
        <li>Transformed DH5α with promoter+RBS+ribitol transporter, ribitol operon+RFP+terminator, Gal1+Improved+pSB1C3, and pRS424 [Cam, Havisha]</li>
 +
        <li>Began overnight cultures of <I>DH5α</I> transformed with Gal1+Improved Part+Tef1 plasmid and Sr35 full construct+AvrSr35 full construct plasmid [Cam]</li>
 +
        <li>Made LB agar + amp plates [Kyle, Cam]</li>
 +
        <li>Tested solar panels and battery charging [Kyle]</li>
 +
     </ul>
 +
  </div>
 
   </div>
 
   </div>
  
Line 2,227: Line 2,280:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Saturday, October 7</u></strong></p>
+
     <p><strong><u>Sunday, October 7</u></strong></p>
−
    <p><em>Present:Mark, Maddie</em></p>
+
  <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Miniprepped Gal1+Improved Part+Tef1 plasmid and Sr35 full construct+AvrSr35 full construct plasmid [Havisha]</li>
−
        <li>Prepared LB Agar <em>[Mark]</em></li>
+
        <li>Worked on ribitol modelling [Diva]</li>
−
        <li>Prepared overnight cultures of BCP, uvsE, and phrAC <em>[Maddie]</em></li>
+
        <li>Worked on device and hardware [Kyle]</li>
−
    </ol>
+
        <li>Made an ran a gel to screen Gal1+Improved Part+Tef1 plasmid and Sr35 full construct+AvrSr35 full construct plasmid [Elizabeth]</li>
−
     <p><strong> Outside Work / Discussion </strong><p>
+
        <li>Set up ligation reaction between Gal1+Improved Part+pSB1C3 [Elizabeth]</li>
 +
        <li>Transformed <I>DH5α</I> with promoter+RBS+ribitol transporter, ribitol operon+RFP+terminator, and pRS424 [Cam, Elizabeth]</li>
 +
        <li>Began overnight cultures of Sr35 full construct+pSB1C3 [Cam]</li>
 +
     </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,241: Line 2,297:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Sunday, October 8</u></strong></p>
+
     <p><strong><u>Monday, October 8</u></strong></p>
−
    <p><em>Present:Mark, Micah</em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Made overnight colonies of Gal1+Improved Part+pSB1C3, promoter+RBS+ribitol transporter plasmid, ribitol operon+RFP+terminator plasmid, and pRS424 [Havisha] </li>
−
        <li>Prepared for a UV irradiation test for uvsE, phrAC, and BCP control <em>[Mark, Micah]</em></li>
+
        <li>Miniprepped Sr35 part 1+pSB1C3, promoter+RBS+ribitol transporter plasmid, and pRS424 [Cam, Havisha]</li>
−
    </ol>
+
        <li>Made and ran gel to screen Sr35 full construct+pSB1C3, promoter+RBS+ribitol transporter plasmid, and pRS424 [Cam, Havisha]</li>
−
    <p><strong> Outside Work / Discussion </strong><p>
+
        <li>Set up digestion reactions for pRS424, pSB1C3, linearized pSB1C3, and RFP+terminator, Sr35 part 1, Tef1 [Cam, Elizabeth, Havisha]</li>
 +
        <li>Made and ran gel to purify pRS424 and RFP insert from pSB1C3 [Cam, Havisha]</li>
 +
        <li>Set up ligation reaction between pRS424 and RFP insert, Sr35 part 1 and Sr35 part 2, and RFP+terminator and ribitol operon [Cam, Havisha]</li>
 +
        <li>Transformed <I>DH5α</I> with RFP+pRS424 plasmid, Sr35 full construct+pSB1C3, Gal1+Improved part+Tef1, and RFP+terminator+ribitol operon plasmid [Havisha]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,254: Line 2,314:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Monday, October 9</u></strong></p>
+
     <p><strong><u>Tuesday, October 9</u></strong></p>
−
    <p><em>Present: Collin</em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Miniprepped Gal1+Improved Part+pSB1C3 [Elizabeth, Havisha]</li>
−
         <li> Measured the OD of the cyanobacteria culture <em>[Maddie]</em></li>
+
         <li>Made and ran gel to screen Gal1+Improved Part+pSB1C3 [Cam, Elizabeth]</li>
−
              <ul>
+
        <li>Began <I>EBY100</I> overnight cultures [Elizabeth]</li>
−
                  <li> 3.1760 (#2)</li>
+
        <li>Began overnight cultures of ribitol operon, Sr35 full construct+pSB1C3,  and RFP+pRS424 plasmid [Cam, Elizabeth]</li>
−
              </ul>            
+
     </ul>
−
    </ol>      
+
−
    </ol>
+
−
     <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,271: Line 2,328:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Tuesday, October 10</u></strong></p>
+
     <p><strong><u>Wednesday, October 10</u></strong></p>
−
    <p><em>Present: Collin</em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Miniprepped ribitol operon, Sr35 full construct+pSB1C3,  and RFP+pRS424 plasmid [Cam, Elizabeth, Havisha]</li>
−
         <li>Digested neomycin phosphotransferase (nptII) and pSB1C3 <em>[Colin]</em></li>
+
     </ul>
−
        <li>Ran a gel electrophoresis for the digested nptII and pSB1C3 <em>[Collin]</em></li>
+
−
        <li>Transformed uvsE+BCP into E. coli <em>[Collin]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,286: Line 2,339:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Wednesday, October 11</u></strong></p>
+
     <p><strong><u>Thursday, October 11</u></strong></p>
−
    <p><em>Present: Collin</em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Set up digestion reactions for Sr35 part1, Sr35 part 2, AvrSr35 full construct, Gal1+Improved part, and pRS424 [Cam, Havisha]</li>
−
         <li>Made Kanamycin plates <em>[Collin]</em></li>
+
         <li>Set up ligation reactions between Sr35 part+part 2, AvrSr35+pRS424, Gal1+Improved part+pRS424 [Elizabeth]</li>
−
         <li>Prepared bacterial stabs of Dsup, phrAC, and phrAT for the UChicago team <em>[Collin]</em></li>
+
     </ul>
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,300: Line 2,351:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Thursday, October 12</u></strong></p>
+
     <p><strong><u>Friday, October 12</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Transformed DH5α with Sr35 part+part 2, AvrSr35+pRS424, Gal1+Improved part+pRS424, and Tef1 [Cam, Elizabeth, Havisha]</li>
 +
        <li>Began overnight cultures of <I>DH5α</I> transformed with Sr35 part+part 2, AvrSr35+pRS424, Gal1+Improved part+pRS424, and Tef1 [Cam, Elizabeth]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,309: Line 2,363:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Friday, October 13</u></strong></p>
+
     <p><strong><u>Saturday, October 13</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Elizabeth</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Miniprepped Sr35 part+part 2, AvrSr35+pRS424, Gal1+Improved part+pRS424, and Tef1 [Cam, Elizabeth]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,318: Line 2,374:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Saturday, October 14</u></strong></p>
+
     <p><strong><u>Sunday, October 14</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Elizabeth</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Made and ran gel to screen Sr35 part+part 2, AvrSr35+pRS424, and Gal1+Improved part+pRS424 [Elizabeth]</li>
 +
        <li>Made overnight cultures of WT <I>DH5α</I> and <I>DH5α</I> transformed with ribitol transporter [Elizabeth, Cam]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Sunday, October 15</u></strong></p>
+
     <p><strong><u>Monday, October 15</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Elizabeth</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Transferred WT <I>DH5α</I> and <I>DH5α</I> into M9 Media [Elizabeth]</li>
 +
        <li>Made competent <I>EBY100</I> [Elizabeth]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,336: Line 2,398:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Monday, October 16</u></strong></p>
+
     <p><strong><u>Tuesday, October 16</u></strong></p>
 
     <p><em>Present: Mark</em></p>
 
     <p><em>Present: Mark</em></p>
−
    <p><strong> Lab Work </strong></p>
+
  <p><em>Present: Elizabeth</em></p>
−
     <ol>
+
     <ul>
−
        <li>Gel purified pSB1C3 and nptII <em>[Mark]</em></li>
+
        <li>Washed and extracted fluid from WT <I>DH5α</I> and <I>DH5α</I> [Elizabeth]</li>
−
        <li>Ligated pSB1C3 and nptII <em>[Mark]</em></li>
+
        <li>Transformed <I>EBY100</I> with improved part full construct and Avr35+Sr35 full construct [Elizabeth]</li>
−
        <li>Transformed pSB1C3+nptII <em>[Mark]</em></li>
+
     </ul>
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,351: Line 2,411:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Tuesday, October 17</u></strong></p>
+
     <p><strong><u>Wednesday, October 17</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Elizabeth, Havisha</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Ran GC-MS samples [Cam, Elizabeth, Havisha]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,360: Line 2,422:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Wednesday, October 18</u></strong></p>
+
     <p><strong><u>Thursday, October 18</u></strong></p>
−
    <p><em></em></p>
+
  <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p>No work done today!</p>
+
     <ul>
 +
        <li>Prepared for Jamboree [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
 +
    </ul>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,369: Line 2,433:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Thursday, October 19</u></strong></p>
+
     <p><strong><u>Friday, October 19</u></strong></p>
−
    <p><em>Present: Collin</em></p>
+
  <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Prepared for Jamboree [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
−
         <li>Streaked phrAT, phrAC, BCP, uvsE, and BCP <em>[Collin]</em></li>
+
     </ul>
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,382: Line 2,444:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Friday, October 20</u></strong></p>
+
     <p><strong><u>Saturday, October 20</u></strong></p>
−
    <p><em>Present: Collin</em></p>
+
  <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Prepared for Jamboree [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
−
         <li>Transformed lac+RBS+Dsup+BCP into E.coli cells <em>[Collin]</em></li>
+
     </ul>
−
        <li>Prepared cultures of lac+RBS+Dsup, lac+RBS+BCP, and lac+RBS+uvsE <em>[Collin]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,396: Line 2,455:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Saturday, October 21</u></strong></p>
+
     <p><strong><u>Sunday, October 21</u></strong></p>
−
    <p><em>Present: Micah</em></p>
+
  <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
         <li>Prepared for Jamboree [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
−
        <li>Transformed medium promoter+medium RBS (BBa_K608006) into E. coli <em>[Micah]</em></li>
+
     </ul>
−
         <li>Prepared cultures from previous transformation <em>[Micah]</em></li>
+
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,410: Line 2,466:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Sunday, October 22</u></strong></p>
+
     <p><strong><u>Monday, October 22</u></strong></p>
−
    <p><em>Present:Collin</em></p>
+
  <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Prepared for Jamboree [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
−
        <li>Made glycerol stocks of lac+RBS+Dsup+RBS+BCP+pSB1C3 <em>[Collin]</em></li>
+
     </ul>
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
Line 2,423: Line 2,477:
 
   <div class="mySlides fade">
 
   <div class="mySlides fade">
 
     <div style="height: 200px; width: 100%;">
 
     <div style="height: 200px; width: 100%;">
−
     <p><strong><u>Monday, October 23</u></strong></p>
+
     <p><strong><u>Tuesday, October 23</u></strong></p>
−
    <p><em>Present: Collin</em></p>
+
  <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p>
−
     <p><strong> Lab Work </strong></p>
+
     <ul>
−
    <ol>
+
        <li>Prepared for Jamboree [Cam, Diva, Elizabeth, Havisha, Kyle]</li>
−
        <li>Miniprepped lac+RBS+BCP+pSB1C3 <em>[Collin]</em></li>
+
     </ul>
−
     </ol>
+
−
    <p><strong> Outside Work / Discussion </strong><p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>

Latest revision as of 22:37, 17 October 2018

June 2018
Sunday Monday Tuesday Wednesday Thursday Friday Saturday
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30

Friday, June 1

No work done today!

Saturday, June 2

No work done today!

Sunday, June 3

No work done today!

Monday, June 4

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Researched different resistance genes to identify ones that are sequenced and applicable to the project [Elizabeth, Diva, Cam]
  • Studied glucosamine detection mechanisms to signal the presence the presence of fungi [Havisha]
  • Planned trip to Uganda and human practices potential abroad [Kyle]

Tuesday, June 5

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Researched different resistance genes to identify ones that are sequenced and applicable to the project [Elizabeth, Diva]
  • Compared different aspects of Sr genes [Cam]
  • Examined a protocol for rapid cloning of plant disease resistance genes [Diva]
  • Studied glucosamine detection mechanisms to signal the presence the presence of fungi [Havisha, Diva]
  • Planned trip to Uganda and potential human practices abroad [Kyle]

Wednesday, June 6

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Examined lengths of resistance gene sequences to begin ordering material [Elizabeth]
  • Studied fungal spore traps to limit specificity to Puccinia graminis [Havisha]
  • Investigated different detection and reporting mechanisms for reporting information with a device [Cam, Diva]
  • Planned trip to Uganda and potential human practices abroad [Kyle]

Thursday, June 7

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Met with Dr. Brennan to redefine project goals and timeline [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Reviewed videos from the BGRI to determine locations of wheat rust fungus prevalence [Elizabeth]
  • Prepared presentation for Microsoft partnership meeting and consulted Dr. Brennan on areas for improvement [Cam]
  • Studied fungal spore traps to limit specificity to Puccinia graminis [Havisha]
  • Continued research on various detection mechanisms [Diva]
  • Planned trip to Uganda and potential human practices abroad [Kyle]

Friday, June 8

Present: Cam, Diva, Elizabeth, Havisha

  • Met with a representative from Microsoft to establish a technology partnership [Cam]
  • Reviewed papers regarding resistance gene interactions (Sr35, Sr22, and Sr45) [Elizabeth]
  • Considered phage related detection mechanisms, alongside the use of CRISPR/CAS9 [Diva]
  • Studied fungal spore traps to select only ones of Puccinia graminis [Havisha]

Saturday, June 9

No work done today!

Sunday, June 10

No work done today!

Monday, June 11

Present: Cam, Diva, Elizabeth, Havisha

  • Prepared a summary of the global impact of rust for Microsoft [Cam]
  • Planned for the use of Sr33 and Sr13 [Elizabeth]
  • Studied compounds unique to germinated P. graminis and infectious structures [Havisha]
  • Investigated the DNA repair mechanism of eukaryotes (fungi in particular) [Diva]

Tuesday, June 12

Present: Cam, Diva, Elizabeth, Havisha

  • Revised our project abstract to incorporate changes made over the past week [Cam, Diva, Elizabeth, Havisha]
  • Conducted a marketing brainstorming session [Cam, Diva, Elizabeth, Havisha]
  • Researched Sr50 and truncating resistance genes [Elizabeth]
  • Compiled a research summary for Microsoft [Cam]
  • Examined different detection mechanisms for ribitol [Havisha]
  • Investigated the DNA repair mechanism of eukaryotes (fungi in particular) [Diva]

Wednesday, June 13

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Met with Dr. Brennan to assess abstract and project design in preparation to meet with Monsanto [Cam, Diva, Elizabeth, Kyle, Havisha]
  • Compiled gene sequences needed for the project [Elizabeth]
  • Began developing the wiki [Havisha]
  • Identified the sequence for a gene induced by ribotin to transfer into a plasmid and transform E. coli with [Havisha]
  • Investigated the DNA repair mechanism of eukaryotes (fungi in particular) [Diva]
  • Planned trip to Uganda and potential human practices abroad [Kyle]

Thursday, June 14

Present: Cam, Diva, Elizabeth, Havisha

  • Connected with many iGEM teams through social media [Elizabeth, Cam]
  • Developed the wiki template and daily notebook log pages [Havisha]
  • Attempted to identify promoter sequences induced by ribotin [Diva]
  • Investigated truncating Sr50 and fusion proteins [Elizabeth]

Friday, June 15

Present: Cam, Diva, Elizabeth, Havisha

  • Developed footer and human practices page of the wiki [Havisha]
  • Conducted a call with a grain farmer for fundraising and human practices efforts [Cam]
  • Organized presentation in preparation for a meeting with Monsanto [Cam]
  • Investigated Sr35 and fusion proteins [Elizabeth]
  • Met with Jeff to discuss logistics of project [Havisha, Elizabeth]

Saturday, June 16

No work done today!

Sunday, June 17

No work done today!

Monday, June 18

Present: Cam, Diva, Elizabeth, Havisha

  • Worked on presentation in preparation for meeting with Monsanto [Cam, Diva, Elizabeth, Havisha]
  • Prepared PBS buffer and LB Agar plates in preparation for interlab [Cam, Diva, Elizabeth, Havisha]
  • Developed technology partnership with Microsoft. [Cam]

Tuesday, June 19

Present: Cam, Diva, Elizabeth, Havisha

  • Met with Dr. Brennan to prepare for Monsanto presentation. [Cam, Diva, Elizabeth, Havisha]
  • Prepared LB buffer and began overnight culture. [Cam, Diva, Elizabeth, Havisha]

Control agar + CM plates made on 6/18

Wednesday, June 20

Present: Cam, Diva, Elizabeth, Havisha

  • Prepared chemically competent DH5α E. coli cells [Cam, Diva, Elizabeth, Havisha]
  • Developed list of protocols for the wiki [Havisha, Diva]

Thursday, June 21

Present: Cam, Diva, Elizabeth, Havisha

  • Toured Monsanto and Pfizer facilities [Cam, Diva, Elizabeth, Havisha]
  • Presented to Monsanto researchers to solicit advice and feedback from people in the field [Cam, Diva, Elizabeth, Havisha]

Friday, June 22

Present: Cam, Diva, Elizabeth, Havisha

  • Identified gene sequences for ribitol metabolism [Havisha]
  • Began investigating LAMP and primers [Diva]
  • Studied protein-protein interactions of CC-NLR proteins [Diva, Elizabeth]
  • Continued with fundraising efforts and coordinating partnership with Monsanto [Cam]

Saturday, June 23

No work done today!

Sunday, June 24

No work done today!

Monday, June 25

Present: Cam, Diva, Havisha

  • Worked on Day 1 of the interlab study protocol [Cam, Diva, Havisha]

Tuesday, June 26

Present: Cam, Diva, Havisha

  • Troubleshooted problems with Day 1 transformation [Cam, Diva, Havisha]
  • Corresponded with Microsoft regarding digital lab notebook [Cam]
  • Solidified gene sequences needed for general detection mechanism [Havisha]
  • Began filling out iGEM safety form part 1 [Diva]

Transformed DH5α from interlab studies day one on 6/25

Wednesday, June 27

Present: Cam, Diva, Havisha

  • Wrote project overview, background, and general detection mechanism summary for wiki [Havisha]
  • Completed safety form [Diva, Havisha]
  • Made LB Agar Media [Diva, Havisha]
  • Started an overnight culture of DH5α [Diva, Havisha]

Thursday, June 28

Present: Diva, Elizabeth, Havisha

  • Submitted safety form [Diva]
  • Plated LB Agar plates [Diva, Elizabeth, Havisha]
  • Made chemically competent DH5α [Diva, Elizabeth, Havisha]
  • Developed banners for wiki pages [Havisha]

Friday, June 29

Present: Diva, Elizabeth, Havisha

  • Worked on members’ introductions for the wiki [Diva, Elizabeth, Havisha]
  • Tested competency of cells prepared on 6/28 [Diva, Elizabeth, Havisha]

Control agar plate made on 6/28

Saturday, June 30

No work done today!

Sunday, July 1

Present: Diva, Havisha

  • Performed calibration protocol for interlab study [Diva, Havisha]

Control agar plate with non-transformed competent cells plated on 6/29

Agar + CM plates with cells transformed with iGEM test kit from 6/29

Monday, July 2

Present: Diva, Elizabeth, Havisha

  • Made LB Agar + CM plates [Diva, Elizabeth, Havisha]
  • Transformed E. coli DH5α for Interlab Protocol Day 1 [Diva, Elizabeth, Havisha]

Tuesday, July 3

Present: Diva, Elizabeth, Havisha

  • Met with Jeff to discuss project goals and progress [Diva, Elizabeth, Havisha]

Wednesday, July 4

Present: Cam, Diva, Elizabeth

  • Worked on Day 2 of interlab protocol [Cam, Diva, Elizabeth]

Thursday, July 5

Present: Cam, Diva, Elizabeth

  • Worked on Day 3 of interlab protocol [Cam, Diva, Elizabeth]
  • Informed of contamination with E. coli and materials [Cam, Diva, Elizabeth]

Friday, July 6

Present: Cam, Diva, Elizabeth, Kyle

  • Made 1L LB Broth [Diva]
  • Made LB Agar + CM plates [Cam, Elizabeth]
  • Made TSS Buffer [Elizabeth, Diva]
  • Looked into GPCRs [Kyle]

Saturday, July 7

Present: Cam, Elizabeth

  • Transformed DH5α cells using competent cell test kit [Cam, Elizabeth]

Sunday, July 8

Present: Elizabeth

  • Checked transformed cell plates for growth [Elizabeth]

Agar + CM plates with cells transformed with iGEM test kit from 7/7

Monday, July 9

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Consulted with Microsoft computing experts on modelling and device [Cam]
  • Finalized ribitol operon sequences to order [Havisha]
  • Studied resistance genes and sequences to order [Elizabeth]

Tuesday, July 10

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Met with Dr. Brennan to discuss project checkpoints and progress [Elizabeth, Havisha, Kyle]
  • Made new LB media [Diva]
  • Made and plated LB agar and LB agar + CM [Diva, Elizabeth, Havisha]
  • Studied resistance genes and sequences to order [Elizabeth]
  • Ordered ribitol operon gene sequences [Havisha]
  • Met with Dr. Parikh and attended a lecture to develop international human practices [Kyle]
  • Began DH5α overnight culture [Diva, Elizabeth, Havisha]

Wednesday, July 11

Present: Diva, Elizabeth, Havisha

  • Plated DH5α cells on LB agar plate to check for plate contamination [Havisha]
  • Checked plated DH5α cells [Diva, Elizabeth]

Agar plate with overnight culture of DH5α from 7/11

Thursday, July 12

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Conducted Day 1 of interlab protocol with competent cells from NEB [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Discussed human practices progress and integration into project [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Checked plated transformed DH5α cells [Diva, Elizabeth]

Agar + CM plates with transformed cells from Day 1 of interlab protocol from 7/12

Friday, July 13

Present: Cam, Diva, Elizabeth, Kyle

  • Conducted Day 2 of interlab protocol [Diva, Elizabeth]
  • Met with Monsanto plant scientists to discuss project design and resistance genes [Diva, Elizabeth, Kyle]
  • Conducted Hour 0 of Day 3 of interlab protocol [Cam, Diva, Elizabeth]
  • Made LB Agar + CM plates [Cam, Diva]

Saturday, July 14

Present: Cam, Diva, Elizabeth, Kyle

  • Conducted Hour 6 of Day 3 of interlab protocol [Diva, Elizabeth, Kyle]
  • Made LB broth [Cam]

Sunday, July 15

No work done today!

Monday, July 16

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Conducted a production meeting to redefine project progress and delegate individual tasks [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Compiling gene sequences for resistance genes [Elizabeth]
  • Met with a representative from the Missouri Coalition for the Environment [Kyle]
  • Worked on device logistics and reached out to possible advisors for development [Kyle]
  • Procured space in WashU makerspace for device prototyping [Cam]
  • Solidified wiki homepage formatting [Havisha]
  • Looked into longevity of E. coli and yeast and possible alternatives to maintaining a live culture in device [Diva]

Tuesday, July 17

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Researched biobrick scars and Gibson assembly as a possible alternative [Elizabeth]
  • Finalized gene sequences for resistance genes [Elizabeth]
  • Began developing poster for EECE interns presentation [Havisha]
  • Ordered ribitol for testing transformed cells [Cam]
  • Contacted Bayer for possible collaborations with education and human practices [Kyle]
  • Worked on device design [Kyle]
  • Explored powdered cells for one time use as an alternative to a live culture in device [Diva]

Wednesday, July 18

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Worked on poster for presentation [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Worked on summary presentation for video conference [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Looked into plasmids and promoter to use with ribitol operon [Havisha]
  • Ordered AvrSr35 gene sequences [Elizabeth]

Thursday, July 19

Present: Cam, Elizabeth, Havisha, Kyle

  • Attended and presented at Southern iGEM video conference [Elizabeth, Havisha, Kyle]
  • Made Agar + CM plates [Cam, Havisha, Kyle]
  • Discussed and consulted grad students regarding golden gate assembly for resistance gene sequences [Cam, Elizabeth, Havisha, Kyle]
  • Worked on poster for presentation [Havisha]

Friday, July 20

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Met with the head of St. Louis Indoor Produce and toured facility for possible collaborations and human practices [Diva, Elizabeth, Havisha, Kyle]
  • Met to discuss and define project timeline and goals [Cam, Diva, Elizabeth, Havisha, Kyle]

Saturday, July 21

Present: Diva, Elizabeth, Havisha, Kyle

  • Conducted a collaborations video call with the ICT Mumbai iGEM team [Elizabeth, Kyle]
  • Attended and presented at North American Kick-off event video conference [Elizabeth, Havisha, Kyle]
  • Transformed DH5α with positive and negative controls for interlab CFU/mL protocol [Diva, Havisha]
  • Checked plates and began four overnight cultures for interlab CFU/mL protocol [Diva]

Agar + CM plates with transformed cells from for CFU interlab protocol from 7/21

Sunday, July 22

Present: Diva, Havisha

  • Conducted dilution and plating steps for interlab CFU/mL protocol [Diva, Havisha]

Monday, July 23

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Conducted a production meeting to redefine project progress and delegate individual tasks [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Met with Dr. Feher to discuss device logistics [Kyle]
  • Counted colonies on plates for interlab CFU/mL protocol [Cam, Diva, Elizabeth, Kyle]
  • Met with Microsoft to touch base on goals for the modeling component of our project [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Met with Dr. Brennan to discuss part assembly and testing of ordered parts [Diva, Elizabeth, Havisha]

Agar + CM plates with diluted cultures of transformed cells from for CFU interlab protocol from 7/22

Tuesday, July 24

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Toured the Danforth Plant Sciences Center [Cam, Elizabeth, Havisha, Kyle]
  • Finished and printed poster [Diva]

Wednesday, July 25

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Presented at EECE Interns Poster Session [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Met with Dr. Kunkel to discuss resistance genes and application of project [Elizabeth, Havisha]
  • Ordered Sr35 genes [Elizabeth]

Thursday, July 26

Present: Cam, Diva, Elizabeth, Kyle

  • Made ampicillin and chloramphenicol [Cam]
  • Made kanamycin [Elizabeth]
  • Made LB+Cam plates [Kyle]
  • Made overnight DH5α cultures [Diva]

Friday, July 27

Present: Diva, Elizabeth, Kyle

  • Made glycerol stocks of DH5α cells [Diva]
  • Made competent DH5α cells [Diva, Elizabeth, Kyle]

Saturday, July 28

Present: Elizabeth, Kyle

  • Ran competent cell test kit [Elizabeth, Kyle]
  • Discussed human practices [Elizabeth, Kyle]

Sunday, July 29

Present: Diva, Elizabeth, Kyle

  • Checked plates from competent cell kit [Elizabeth]
  • Worked on wiki content for global human practices [Diva, Kyle]

Monday, July 30

Present: Diva, Elizabeth, Kyle

  • Grew an overnight culture of DH5α cells [Diva]
  • Transformed NEB competent DH5α cells with pSB1C3 (pre-miniprep) [Elizabeth]
  • Tested for competent cells [Kyle]
  • Met with Dr. Brennan to discuss part assembly and competent cell preparation protocol [Diva, Elizabeth, Kyle]
  • Met with Jeff and other grad students to discuss preventing contamination and improving efficacy of interlab technique [Diva, Elizabeth, Kyle]

Tuesday, July 31

Present: Diva, Elizabeth, Kyle

  • Made competent DH5α cells [Kyle, Elizabeth]
  • Tested TSS and SOC media pH with glass pH probe [Elizabeth, Diva]

Cells transformed with pSB1C3 from 7/30

Results from competent cell test kit from 7/30

Wednesday, August 1

Present: Diva

  • Made LB agar plates [Diva]

Thursday, August 2

Present: Cam, Diva, Elizabeth, Kyle

  • Made competent DH5α cells [Cam, Diva]
  • Transformed competent cells with RFP [Kyle, Elizabeth]

Friday, August 3

Present: Cam, Diva, Elizabeth, Kyle

  • Transformed competent cells with Part BBa_K2663000 [Elizabeth, Kyle]
  • Made LB agar + CM plates [Diva, Cam]
  • Made overnight cultures of cells transformed with RFP [Cam]

Saturday, August 4

Present: Elizabeth, Kyle

  • Made LB [Elizabeth, Kyle]
  • Made glycerol stocks of overnight cultures [Elizabeth, Kyle]
  • Plated cells to check for contamination [Elizabeth, Kyle]
  • Ordered miniprep kit [Elizabeth, Kyle]
  • Checked plates to ensure lack of contamination [Elizabeth, Kyle]
  • Plated overnight cultures [Elizabeth, Kyle]

Sunday, August 5

Present: Elizabeth

  • Made glycerol stocks from overnight cultures [Elizabeth]
  • Miniprepped RFP, ribitol component promoter, and RBS [Elizabeth]
  • Autoclaved new tips and microcentrifuge tubes [Elizabeth]
  • Made a negative control to test for contamination in the culture tubes [Elizabeth]

Monday, August 6

Present: Cam, Diva, Elizabeth, Kyle

  • Made ampicillin plates [Cam, Diva]
  • Measured DNA concentrations using nanodrop [Cam, Diva, Elizabeth, Kyle]
  • Made more ampicillin, chloramphenicol, and kanamycin [Cam, Diva]
  • Plated pRSII424 on ampicillin plate [Cam, Diva]

Tuesday, August 7

Present: Cam, Diva, Elizabeth, Kyle

  • Made an overnight culture of pRSII424 [Cam, Elizabeth, Kyle]

Wednesday, August 8

Present: Elizabeth, Havisha, Kyle

  • Minipreped pRSII424 plasmid [Elizabeth, Havisha, Kyle]
  • Performed PCR on ribitol operon, ribitol transporter, and AvrSr35 [Elizabeth, Havisha, Kyle]
  • Began an overnight culture of DH5α cells [Elizabeth, Havisha]

Thursday, August 9

Present: Cam, Havisha, Kyle

  • Conducted collaboration video calls with Cardiff, Westminster, and University of Minnesota iGEM teams [Havisha, Kyle]
  • Made competent DH5α cells [Cam]
  • Performed PCR on yeast plasmid backbone [Havisha]
  • Made and ran gel electrophoresis with PCR products [Havisha]

Friday, August 10

Present: Elizabeth, Havisha

  • Performed PCR on ribitol operon, ribitol transporter, and AvrSr35 [Havisha]
  • Made and ran gel electrophoresis with PCR products [Havisha]
  • Transformed DH5α cells with mini prepped pSB1C3 plasmid with RFP [Elizabeth]
  • Transformed DH5α cells with YFP Part BBa_I6031[Elizabeth]

Saturday, August 11

Present: Cam, Havisha

  • Purified ribitol transporter and AvrSr35 from agarose gel [Cam, Havisha]
  • Performed PCR on ribitol operon, ribitol transporter, and pRSII424 plasmid [Cam, Havisha]
  • Made and ran gel electrophoresis with PCR products [Cam, Havisha]

Cells transformed with pSB1C3 from 8/10

Sunday, August 12

Present: Cam, Havisha

  • Performed PCR on ribitol operon and ribitol transporter [Havisha]
  • Made SOC Media [Cam]
  • Purified pRSII424 plasmid from agarose gel [Cam, Havisha]
  • Transformed DH5α cells with pSB1A3 plasmid [Cam, Havisha]
  • Transformed DH5α cells with pSB1K3 plasmid [Cam, Havisha]
  • Transformed DH5α cells with YFP Part BBa_I6031 [Cam, Havisha]

Monday, August 13

Present: Cam, Havisha

  • Began an overnight culture of cells transformed with pSB1A3 plasmid [Havisha]
  • Made LB agar + Kanamycin plates [Cam, Havisha]
  • Transformed DH5α cells with pSB1K3 plasmid [Cam, Havisha]
  • Transformed DH5α cells with YFP Part BBa_I6031 [Cam, Havisha]

Cells transformed with pSB1A3 from 8/12

Cells transformed with YFP from 8/12

Tuesday, August 14

Present: Cam, Havisha

  • Made LB + Amp Media [Cam, Havisha]
  • Made LB + Kan Media [Cam, Havisha]
  • Began an overnight culture of DH5α cells with pSB1K3 plasmid [Cam, Havisha]
  • Began an overnight culture of DH5α cells with YFP Part BBa_I6031 [Cam, Havisha]
  • Began an overnight culture of DH5α cells with pSB1A3 plasmid [Cam, Havisha]

Cells transformed with pSB1K3 from 8/13

Cells transformed with YFP from 8/13

Wednesday, August 15

Present: Cam, Elizabeth, Havisha, Kyle

  • Miniprepped pSB1K3 plasmid [Cam, Elizabeth, Havisha]
  • Miniprepped YFP Part BBa_I6031 [Cam, Elizabeth, Havisha]
  • Miniprepped pSB1A3 plasmid [Cam, Elizabeth, Havisha]
  • Worked on arduino coding for device [Kyle]
  • Transformed electrocompetent DH5α cells for practice [Cam, Elizabeth, Havisha, Kyle]

Overnight cultures of YFP, pSB1A3 plasmid, and pSB1K3 plasmid from 8/14

Thursday, August 16

Present: Cam, Elizabeth, Havisha, Kyle

  • Met with Dr. Shah from the Danforth Plant Science Center [Cam, Elizabeth, Havisha, Kyle]
  • Transformed DH5α cells for Day 1 of the interlab protocol [Cam, Elizabeth, Havisha, Kyle]
  • Transformed electrocompetent DH5α cells for practice [Cam, Elizabeth, Havisha, Kyle]

Friday, August 17

Present: Cam, Elizabeth, Kyle

  • Attended the iGEM Midwest Meetup [Cam, Elizabeth]
  • Started overnight cultures for Day 2 of the interlab protocol [Kyle]

Saturday, August 18

Present: Cam, Elizabeth, Kyle

  • Started overnight cultures for Day 2 of the interlab protocol [Elizabeth]
  • Transformed DH5α cells with positive control, negative control, and test device two for Day 1 of the interlab protocol [Cam, Elizabeth, Kyle]

Sunday, August 19

Present: Cam, Elizabeth

  • Made overnight cultures of positive control, negative control, and test device two for Day 2 of the interlab protocol [Cam]
  • Performed part three of the interlab calibration protocol [Elizabeth]
  • Made LB agar and chloramphenicol plates [Elizabeth]

Monday, August 20

Present: Cam, Elizabeth, Kyle

  • Finalized sequencing primers [Elizabeth]
  • Transformed DH5α cells with positive control, test device one, test device two, and test device three for Day 1 of the interlab protocol [Cam, Elizabeth, Kyle]
  • Made LB agar and chloramphenicol plates [Cam, Elizabeth]
  • Transformed DH5α cells with YFP [Kyle]

Tuesday, August 21

Present: Cam, Elizabeth, Kyle

  • Transformed DH5α cells with Gal1, Tef2, and RFP + terminator [Cam, Elizabeth, Kyle]

Wednesday, August 22

Present: Cam, Kyle

  • Autoclaved water and culture tubes [Cam, Kyle]
  • Made overnight cultures of Gal1, Tef2, and RFP + terminator [Cam]

Thursday, August 23

Present: Cam

  • Made overnight cultures of for Day 2 of the interlab protocol [Cam]

Friday, August 24

Present: Elizabeth, Havisha, Kyle

  • Performed hour zero preparations for Interlab Day 3 [Havisha]
  • Performed hour six for Interlab Day 3 [Elizabeth]
  • Set up digestion reactions for Sr35 part 1, Sr35 part 2, GAL1, and TEF1 [Elizabeth]
  • Ran gel with Sr35 part 1, Sr35 part 2, GAL1, and TEF1 [Elizabeth, Havisha]
  • Performed PCR for AvrSr35 [Havisha]
  • Autoclaved tips [Kyle]

Saturday, August 25

Present: Havisha, Kyle

  • Purified GAL1 and TEF1 from gel [Havisha]
  • Performed PCR for AvrSr35 [Kyle]
  • Ran gel with AvrSr35 PCR product to confirm fragment size [Havisha]

Sunday, August 26

No work done today!

Monday, August 27

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Met with Dr. Brennan to discuss project progress, logistics, and possible troubleshooting [Cam, Diva, Elizabeth, Havisha, Kyle]

Tuesday, August 28

Present: Elizabeth, Havisha, Kyle

  • Performed PCR for pRSII424 plasmid [Elizabeth]
  • Set up digestion reactions for ribitol operon and ribitol transporter [Elizabeth, Kyle]
  • Set up digestion reactions for RFP + terminator and promoter + RBS [Elizabeth, Havisha]
  • Made and ran gel with ribitol operon and ribitol transporter [Elizabeth, Havisha]

Wednesday, August 29

Present: Cam, Havisha

  • Purified ribitol transporter from gel [Havisha]
  • Made and ran gel to confirm pRSII424 plasmid [Cam, Havisha]
  • Purified digestion reactions for RFP + terminator [Cam, Havisha]

Thursday, August 30

Present: Cam, Elizabeth, Havisha, Kyle

  • Made and ran gel to confirm pRSII424 plasmid and AvrSr35 [Cam, Elizabeth, Havisha]
  • Set up ligation reaction for ribitol transporter and RFP + terminator [Cam, Elizabeth, Havisha]
  • Transformed DH5α cells with ligation products [Cam, Elizabeth, Kyle]

Friday, August 31

Present: Elizabeth

  • Checked transformation plates [Elizabeth]

Saturday, September 1

No work done today!

Sunday, September 2

Present: Elizabeth

  • Checked transformation plates [Elizabeth]

Monday, September 3

Present: Elizabeth

  • Made PCR Mix [Elizabeth]
  • Performed PCR for Sr35 part 1, AvrSr35, RFP + terminator, ribitol operon, promoter + RBS, and ribiol transporter [Elizabeth]
  • Made and ran gel with Sr35 part 1, AvrSr35, RFP + terminator, ribitol operon, promoter + RBS, and ribiol transporter [Elizabeth]
  • Set up digestion reaction for Sr35 part 1, AvrSr35, RFP + terminator, ribitol operon, promoter + RBS, and ribiol transporter [Elizabeth]

Tuesday, September 4

Present: Cam, Elizabeth, Havisha

  • Performed PCR for ribitol operon and ribitol transporter [Elizabeth]
  • Set up digestion reactions for RFP + terminator, ribitol operon, promoter + RBS, and ribiol transporter [Elizabeth]
  • Made and ran gel with RFP + terminator, ribitol operon, promoter + RBS, and ribiol transporter [Cam, Elizabeth]
  • Made and ran gel with AvrSr35, Sr35 part 1, RFP + terminator, yeast plasmid, and promoter + RBS [Elizabeth]
  • Purified AvrSr35, Sr35 part 1, yeast plasmid, and promoter + RBS from gel [Havisha]

Wednesday, September 5

Present: Elizabeth, Havisha

  • Set up digestion reactions for Tef1 and RFP + terminator [Havisha]
  • Made and ran gel with Tef1 and RFP + terminator [Havisha]
  • Purified Tef1 and RFP + terminator from gel [Elizabeth, Havisha]
  • Set up digestion reaction for Tef1 [Elizabeth]
  • Made and ran gel with Tef1 and cut out bands [Elizabeth, Havisha]

Thursday, September 6

Present: Cam, Elizabeth, Havisha, Kyle

  • Purified Tef 1 from gel [Elizabeth]
  • Set up digestion reaction for AvrSr35 and Sr35 part 1 [Elizabeth]
  • Made and ran gel with AvrSr35 and Sr35 part 1 [Havisha, Kyle]
  • Made LB agar and ampicillin plates [Havisha, Kyle]
  • Purified AvrSr35 and Sr35 part 1 from gel [Elizabeth]
  • Set up ligation reaction for AvrSr35 + Gal1 and Sr35 + Gal1 [Havisha]
  • Transformed DH5α cells with ligation products [Cam, Havisha]

Friday, September 7

Present:Cam, Diva, Elizabeth, Havisha, Kyle

  • Met to discuss goals for the week and progress [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Checked plates [Havisha]

Saturday, September 8

Present: Elizabeth

  • Performed PCR on ribitol transporter, AvrSr35, pRS424 plasmid, Sr35 [Elizabeth]

Sunday, September 9

Present: Cam, Diva, Elizabeth, Havisha

  • Set up digestion reactions for pRS424 plasmid and pSB1K3 plasmid [Elizabeth, Havisha]
  • Made and ran gel for pRS424 plasmid [Elizabeth, Havisha]
  • Purified pRS424 plasmid from gel [Elizabeth, Havisha]
  • Made and ran gel for pSB1K3 plasmid [Havisha]
  • pSB1K3 plasmid’s RFP insert from gel [Havisha]
  • Set up ligation reaction between pRS424 plasmid and RFP insert [Cam]
  • Transformed DH5α cells with ligation products [Cam]

Monday, September 10

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Met to discuss goals for the week and progress [Cam, Diva, Elizabeth, Havisha, Kyle]
  • Checked plates [Elizabeth]

Tuesday, September 11

Present: Cam, Elizabeth, Havisha, Kyle

  • Met to edit abstract [Cam, Elizabeth, Havisha, Kyle]
  • Made and ran gel for ribitol transporter [Elizabeth]
  • Set up digestion for AvrSr35 [Elizabeth]
  • PCR purified digestion of AvrSr35 [Elizabeth, Havisha]
  • Set up ligation reaction between AvrSr35 and Gal1 [Elizabeth, Havisha]

Wednesday, September 12

Present: Elizabeth, Havisha, Kyle

  • Set up digestion for Sr35 part 1 [Elizabeth]
  • PCR purified digestion of Sr35 part 1 [Elizabeth, Havisha]
  • Set up ligation reaction between Sr35 part 1 and Gal1 [Havisha]
  • Transformed DH5α cells with ligation products [Havisha, Kyle]

Thursday, September 13

Present: Elizabeth

  • Checked plates [Elizabeth]

Friday, September 14

Present: Elizabeth, Havisha, Kyle

  • Performed PCR on pRS424 plasmid [Elizabeth]
  • Set up digestion for AvrSr35, pRS424 plasmid, ribitol transporter, and Sr35 part 1 [Elizabeth]
  • PCR purified AvrSr35, pRS424 plasmid, ribitol transporter, and Sr35 [Havisha]
  • Plated cells transformed with pSB1C3 [Kyle]
  • Set up digestion for promoter + RBS, ribitol transporter, and RFP + terminator [Elizabeth]
  • Organized parts and enzymes inventory [Elizabeth, Kyle]

Saturday, September 15

Present: Elizabeth, Havisha, Kyle

  • Checked plates [Havisha]
  • PCR purified promoter + RBS, ribitol transporter, and RFP + terminator [Havisha]
  • Began overnight cultures of pSB1C3 [Kyle]
  • PCR purified AvrSr35, Sr35 part 1, and pRS424 plasmid [Havisha]
  • Set up digestion reactions for Sr35 part 1, pRS424 plasmid, pSB1C3, and promoter + RBS [Havisha]
  • Set up ligation reaction between Sr35 part 1 and Gal1 and pRS424 plasmid and RFP insert from pSB1C3 [Havisha]
  • Performed PCR on ribitol operon and ribitol transporter [Elizabeth]

Plated cells transformed with pSB1C3 from 9/14

Monday, September 16

Present: Elizabeth, Havisha

  • Miniprepped pSB1C3 [Elizabeth]
  • PCR purified ribitol transporter and AvrSr35 [Havisha]
  • Performed PCR on AvrSr35 [Elizabeth, Havisha]
  • Made and ran gel with ribitol operon [Havisha]
  • Set up digestion reactions for ribitol transporter and RFP + terminator [Havisha]
  • Set up ligation reaction between Sr35 part 1 and Gal1, pRS424 plasmid and RFP insert from pSB1C3, ribitol transporter and promoter + RBS, and RFP + terminator
  • Transformed DH5α cells with ligation products and pSB1C3

Overnight cultures of pSB1C3 from 9/15

Monday, September 17

Present: Cam, Elizabeth, Havisha, Kyle

  • Met to discuss project progress and timeline [Cam, Elizabeth, Havisha, Kyle]
  • Made and ran a gel with ribitol operon PCR product [Havisha, Kyle]
  • Began overnight cultures of DH5α transformed with Sr35 part 1 and Gal1 plasmid, pRS424 plasmid with RFP insert, and ribitol transporter and promoter + RBS plasmid [Havisha, Kyle]
  • Set up digestion reaction for AvrSr35 [Havisha]
  • Set up ligation reaction between AvrSr35 and Gal1 [Havisha]
  • Transformed DH5α with ligation products [Cam]
  • Made chloramphenicol plates [Cam]

Plated cells transformed with ligation products from 9/16

Tuesday, September 18

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Miniprepped Sr35 part 1 and Gal1 plasmid, pRS424 plasmid with RFP insert, and ribitol transporter and promoter + RBS plasmid [Cam, Elizabeth, Havisha]
  • Made and ran gels with miniprepped plasmids [Elizabeth, Havisha, Kyle, Diva]
  • Conducted a collaboration call with Cardiff regarding hardware, wiki, and parts collaboration [Havisha, Kyle]

Gel with ligation products

Wednesday, September 19

Present: Cam, Elizabeth, Havisha

  • Made and ran a gel with ribitol transporter and promoter + RBS plasmid [Cam, Elizabeth, Havisha]
  • Set up digestion reaction for Sr35 part 2, Gal1, ribitol transporter, Sr35 part 1 + Gal1 plasmid, and promoter+RBS [Elizabeth]
  • Set up ligation reaction between AvrSr35 and Gal1 promoter, Sr35 part 2 and Sr35 part 1 + Gal1 plasmid [Cam, Havisha]
  • Transformed DH5α with ligation products and RFP + terminator [Cam]

Thursday, September 20

Present: Elizabeth, Kyle

  • Began overnight cultures of DH5α transformed with AvrSr35 + Gal 1 plasmid [Kyle]
  • Set up digestion reaction for Sr35 part 2 [Elizabeth]
  • Set up ligation reaction between Sr35 part 2 and Sr35 part 1 + Gal1 plasmid [Elizabeth]

Friday, September 21

Present: Cam, Elizabeth, Havisha

  • Miniprepped AvrSr35 plasmid [Havisha]
  • Made and ran gel to screen AvrSr35 plasmid and pRS424 plasmid with RFP insert [Elizabeth, Havisha, Cam]
  • Performed PCR on ribitol operon [Elizabeth]
  • Set up digestion reactions for AvrSr35 and Tef1 [Cam, Elizabeth]
  • Set up ligation reaction between AvrSr35 and Tef1 [Cam, Elizabeth]
  • Transformed DH5α with AvrSr35 + Tef1 plasmid [Cam, Elizabeth]

Saturday, September 22

Present: Kyle

  • Began overnight cultures of Sr35 full construct and AvrSr35 + Tef1 plasmids [Kyle]

Sunday, September 23

Present: Diva, Kyle

  • Miniprepped Sr35 full construct and AvrSr35 + Tef1 plasmids [Diva, Kyle]

Monday, September 24

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Met to discuss project progress and timeline [Diva, Elizabeth, Havisha, Kyle]
  • Performed PCR on ribitol operon [Elizabeth]
  • Made and ran gel to screen Sr35 full construct and AvrSr35 + Tef1 plasmids [Cam, Elizabeth, Kyle]

Tuesday, September 25

Present: Cam, Elizabeth, Havisha

  • Made YPD media [Cam, Elizabeth]
  • Performed PCR on ribitol transporter [Elizabeth]
  • PCR purified ribitol transporter [Elizabeth]
  • Set up digestion reactions for pRS424 plasmid, Gal1, Sr35 full construct, and AvrSr35 full construct [Elizabeth]
  • Began overnight culture of promoter + RBS [Elizabeth]
  • Set up ligation reactions between pRS424 plasmid and RFP insert, pRS424 plasmid and Sr35 construct, pRS424 plasmid and Gal1 promoter, and pRS424 plasmid and AvrSr35 full construct [Elizabeth, Havisha]
  • Transformed DH5α with ligation products [Havisha]

Wednesday, September 26

Present: Cam, Havisha

  • Miniprepped promoter + RBS [Cam, Havisha]
  • Set up digestion reaction for promoter + RBS [Havisha]
  • PCR purified promoter + RBS [Cam, Havisha]
  • Set up ligation reaction between Sr35 full construct and pSB1C3 [Cam, Havisha]
  • Set up ligation reaction between promoter + RBS and ribitol transporter [Cam]
  • Transformed DH5α with Sr35 full construct + pSB1c3, promoter+RBS + ribitol transporter plasmid, and unknowns 1, 2, and 3 [Cam]
  • Performed PCR on ribitol operon [Cam]
  • Made YPD media [Cam]
  • Began overnight cultures of pRS424 plasmid + AvrSr35 full construct, pRS424 plasmid + RFP, pRS424 plasmid + Gal1, and pRS424 plasmid + Sr35 full construct [Cam]

Thursday, September 27

Present: Havisha, Kyle

  • Miniprepped pRS424 plasmid + AvrSr35 full construct, pRS424 plasmid + RFP, pRS424 plasmid + Gal1, and pRS424 plasmid + Sr35 full construct [Havisha]
  • Began overnight cultures of promoter + RBS + ribitol transporter plasmid and Unknowns 1, 2, and 3 [Kyle]
  • Made YPD agar media [Kyle]
  • Made LB media [Kyle]

Friday, September 28

Present: Cam, Elizabeth

  • Set up digestion and ligation reactions for AvrSr35 and pSB1C3 plasmid [Cam, Elizabeth]
  • Set up ligation reaction between Sr35 full construct and pSB1C3 [Cam, Elizabeth]
  • Transformed DH5α with Sr35+pSB1C3 and AvrSr35+pSB1C3
  • Began overnight cultures of promoter + RBS + ribitol transporter plasmid and Unknowns 1, 2, and 3 [Cam, Elizabeth]
  • Plated S. cerevisiae EBY100 [Cam, Elizabeth]

Saturday, September 29

Present: Cam, Elizabeth

  • Miniprepped promoter + RBS + ribitol transporter plasmid and Unknowns 1, 2, and 3 [Cam]
  • Began overnight cultures of Sr35+pSB1C3 and AvrSr35+pSB1C3 [Elizabeth]

Sunday, September 30

Present: Cam, Elizabeth

  • Miniprepped Sr35+pSB1C3 and AvrSr35+pSB1C3 [Cam]
  • Made and ran gel to screen Sr35+pSB1C3 and AvrSr35+pSB1C3 [Elizabeth]

Monday, October 1

Present: Cam, Elizabeth, Havisha, Kyle

  • Met to discuss project progress and timeline [Cam, Kyle]
  • Made and ran gel to screen ribitol operon PCR, pSB1C3 plasmid + Sr35 full construct, promoter + RBS + ribitol transporter plasmid, and pRS424 plasmid + RFP [Elizabeth, Havisha]
  • Made and ran gel to screen pSB1C3 plasmid + AvrSr35 full construct, RFP + term plasmid, and pRS424 plasmid + AvrSr35 full construct [Havisha]

Tuesday, October 2

Present: Cam, Elizabeth, Havisha

  • Made and ran gels to screen pRS424 plasmid + RFP, pRS424 plasmid + AvrSr35 full construct, pSB1C3 plasmid + Sr35 full construct, and pSB1C3 plasmid + AvrSr35 full construct [Elizabeth, Havisha]
  • Set up digestion reactions for Tef1, pRS424 plasmid, RFP, pSB1C3 plasmid + AvrSr35, Sr35 full construct, Improved part, and Gal1 [Elizabeth]
  • PCR purified digestions [Elizabeth]
  • Set up ligation reactions between Tef1 and pRS424 plasmid, RFP and pRS424 plasmid, pSB1C3 plasmid + AvrSr35 and Sr35 full construct, and Improved part and Gal1 [Elizabeth, Havisha]
  • Transformed DH5α with ligation products [Cam]
  • Made LB agar + CM plates [Cam, Havisha]

Wednesday, October 3

Present: Havisha

  • Made overnight cultures of DH5α transformed with Tef1 and pRS424 plasmid, RFP and pRS424 plasmid, pSB1C3 plasmid + AvrSr35 and Sr35 full construct, and Improved part and Gal1 [Havisha]

Thursday, October 4

Present: Cam, Elizabeth, Havisha

  • Miniprepped Tef1 and pRS424 plasmid, RFP and pRS424 plasmid, pSB1C3 plasmid + AvrSr35 and Sr35 full construct, and Improved part and Gal1 [Cam, Elizabeth, Havisha]
  • Made and ran gel to screen Tef1 and pRS424 plasmid, RFP and pRS424 plasmid, pSB1C3 plasmid + AvrSr35 and Sr35 full construct, and Improved part and Gal1 [Elizabeth, Havisha]

Friday, October 5

Present: Cam, Elizabeth, Havisha

  • Made and ran a gel to screen pRS424 plasmid + RFP [Cam, Elizabeth, Havisha]
  • Set up digestion reactions for Gal1+Improved Part, Tef1, pSB1C3, Sr35 full construct, and AvrSr35 full construct [Cam, Elizabeth, Havisha]
  • PCR purified Gal1+Improved Part, Tef1, pSB1C3, Sr35 full construct, and AvrSr35 full construct [Havisha]
  • Set up ligation reactions between Gal1+Improved Part and Tef1, and Sr35 full construct and AvrSr35 full construct [Elizabeth]

Saturday, October 6

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Transformed DH5α with Gal1+Improved Part+Tef1 plasmid, and Sr35 full construct+AvrSr35 full construct plasmid [Havisha, Kyle]
  • Set up digestion reactions for promoter+RBS, ribitol transporter, RFP+terminator, ribitol operon, and Gal1+Improved Part [Cam, Elizabeth, Havisha]
  • PCR purified promoter+RBS, ribitol transporter, RFP+term, ribitol operon, and Gal1+Improved Part [Elizabeth, Havisha]
  • Ligate promoter+RBS and ribitol transporter, ribitol operon and RFP+terminator, and Gal1+Improved Part and pSB1C3 [Cam, Elizabeth]
  • Transformed DH5α with promoter+RBS+ribitol transporter, ribitol operon+RFP+terminator, Gal1+Improved+pSB1C3, and pRS424 [Cam, Havisha]
  • Began overnight cultures of DH5α transformed with Gal1+Improved Part+Tef1 plasmid and Sr35 full construct+AvrSr35 full construct plasmid [Cam]
  • Made LB agar + amp plates [Kyle, Cam]
  • Tested solar panels and battery charging [Kyle]

Sunday, October 7

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Miniprepped Gal1+Improved Part+Tef1 plasmid and Sr35 full construct+AvrSr35 full construct plasmid [Havisha]
  • Worked on ribitol modelling [Diva]
  • Worked on device and hardware [Kyle]
  • Made an ran a gel to screen Gal1+Improved Part+Tef1 plasmid and Sr35 full construct+AvrSr35 full construct plasmid [Elizabeth]
  • Set up ligation reaction between Gal1+Improved Part+pSB1C3 [Elizabeth]
  • Transformed DH5α with promoter+RBS+ribitol transporter, ribitol operon+RFP+terminator, and pRS424 [Cam, Elizabeth]
  • Began overnight cultures of Sr35 full construct+pSB1C3 [Cam]

Monday, October 8

Present: Cam, Elizabeth, Havisha

  • Made overnight colonies of Gal1+Improved Part+pSB1C3, promoter+RBS+ribitol transporter plasmid, ribitol operon+RFP+terminator plasmid, and pRS424 [Havisha]
  • Miniprepped Sr35 part 1+pSB1C3, promoter+RBS+ribitol transporter plasmid, and pRS424 [Cam, Havisha]
  • Made and ran gel to screen Sr35 full construct+pSB1C3, promoter+RBS+ribitol transporter plasmid, and pRS424 [Cam, Havisha]
  • Set up digestion reactions for pRS424, pSB1C3, linearized pSB1C3, and RFP+terminator, Sr35 part 1, Tef1 [Cam, Elizabeth, Havisha]
  • Made and ran gel to purify pRS424 and RFP insert from pSB1C3 [Cam, Havisha]
  • Set up ligation reaction between pRS424 and RFP insert, Sr35 part 1 and Sr35 part 2, and RFP+terminator and ribitol operon [Cam, Havisha]
  • Transformed DH5α with RFP+pRS424 plasmid, Sr35 full construct+pSB1C3, Gal1+Improved part+Tef1, and RFP+terminator+ribitol operon plasmid [Havisha]

Tuesday, October 9

Present: Cam, Elizabeth, Havisha

  • Miniprepped Gal1+Improved Part+pSB1C3 [Elizabeth, Havisha]
  • Made and ran gel to screen Gal1+Improved Part+pSB1C3 [Cam, Elizabeth]
  • Began EBY100 overnight cultures [Elizabeth]
  • Began overnight cultures of ribitol operon, Sr35 full construct+pSB1C3, and RFP+pRS424 plasmid [Cam, Elizabeth]

Wednesday, October 10

Present: Cam, Elizabeth, Havisha

  • Miniprepped ribitol operon, Sr35 full construct+pSB1C3, and RFP+pRS424 plasmid [Cam, Elizabeth, Havisha]

Thursday, October 11

Present: Cam, Elizabeth, Havisha

  • Set up digestion reactions for Sr35 part1, Sr35 part 2, AvrSr35 full construct, Gal1+Improved part, and pRS424 [Cam, Havisha]
  • Set up ligation reactions between Sr35 part+part 2, AvrSr35+pRS424, Gal1+Improved part+pRS424 [Elizabeth]

Friday, October 12

Present: Cam, Elizabeth, Havisha

  • Transformed DH5α with Sr35 part+part 2, AvrSr35+pRS424, Gal1+Improved part+pRS424, and Tef1 [Cam, Elizabeth, Havisha]
  • Began overnight cultures of DH5α transformed with Sr35 part+part 2, AvrSr35+pRS424, Gal1+Improved part+pRS424, and Tef1 [Cam, Elizabeth]

Saturday, October 13

Present: Cam, Elizabeth

  • Miniprepped Sr35 part+part 2, AvrSr35+pRS424, Gal1+Improved part+pRS424, and Tef1 [Cam, Elizabeth]

Sunday, October 14

Present: Cam, Elizabeth

  • Made and ran gel to screen Sr35 part+part 2, AvrSr35+pRS424, and Gal1+Improved part+pRS424 [Elizabeth]
  • Made overnight cultures of WT DH5α and DH5α transformed with ribitol transporter [Elizabeth, Cam]

Monday, October 15

Present: Elizabeth

  • Transferred WT DH5α and DH5α into M9 Media [Elizabeth]
  • Made competent EBY100 [Elizabeth]

Tuesday, October 16

Present: Mark

Present: Elizabeth

  • Washed and extracted fluid from WT DH5α and DH5α [Elizabeth]
  • Transformed EBY100 with improved part full construct and Avr35+Sr35 full construct [Elizabeth]

Wednesday, October 17

Present: Cam, Elizabeth, Havisha

  • Ran GC-MS samples [Cam, Elizabeth, Havisha]

Thursday, October 18

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Prepared for Jamboree [Cam, Diva, Elizabeth, Havisha, Kyle]

Friday, October 19

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Prepared for Jamboree [Cam, Diva, Elizabeth, Havisha, Kyle]

Saturday, October 20

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Prepared for Jamboree [Cam, Diva, Elizabeth, Havisha, Kyle]

Sunday, October 21

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Prepared for Jamboree [Cam, Diva, Elizabeth, Havisha, Kyle]

Monday, October 22

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Prepared for Jamboree [Cam, Diva, Elizabeth, Havisha, Kyle]

Tuesday, October 23

Present: Cam, Diva, Elizabeth, Havisha, Kyle

  • Prepared for Jamboree [Cam, Diva, Elizabeth, Havisha, Kyle]

Wednesday, October 24

Giant Jamboree!

Thursday, October 25

Giant Jamboree!

Friday, October 26

Giant Jamboree!

Saturday, October 27

Giant Jamboree!

Sunday, October 28

Giant Jamboree!

Monday, October 29

No work done today!

Tuesday, October 30

No work done today!

Wednesday, October 31

No work done today!