Difference between revisions of "Team:HUST-China/Parts Overview"
(Created page with "<html lang="en"> <head> <meta charset="utf-8"> <title>HillSide Multi purpose HTML5 Template</title> <meta name="viewport" content="width=device-width, initial-scale=1.0" /> <m...") |
|||
| (2 intermediate revisions by 2 users not shown) | |||
| Line 160: | Line 160: | ||
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/wetlab/protocols">Protocols</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/wetlab/protocols">Protocols</a></li> | ||
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/InterLab">Interlab</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/InterLab">Interlab</a></li> | ||
| − | |||
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Notebook">Notebook</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Notebook">Notebook</a></li> | ||
</ul> | </ul> | ||
| Line 169: | Line 168: | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Modeling overview">Modeling overview</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Modeling overview">Modeling overview</a></li> | ||
| − | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/ | + | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/model_of_systems">Model of systems</a></li> |
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Sort of three genes">Sort of three genes</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Sort of three genes">Sort of three genes</a></li> | ||
| − | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/ | + | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Software">Software</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
| Line 180: | Line 179: | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Parts Overview">Parts Overview</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Parts Overview">Parts Overview</a></li> | ||
| − | |||
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Composite part">Composite part</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Composite part">Composite part</a></li> | ||
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Part collection">Part collection</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Part collection">Part collection</a></li> | ||
| Line 189: | Line 187: | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Human Practices">Human Practices</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Human Practices">Human Practices</a></li> | ||
| − | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/ | + | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Education_Engagement">Education&Engagement</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
| Line 195: | Line 193: | ||
<a href="#" data-toggle="dropdown" class="dropdown-toggle waves-effect waves-dark">TEAM<b class="caret"></b></a> | <a href="#" data-toggle="dropdown" class="dropdown-toggle waves-effect waves-dark">TEAM<b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| − | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Team | + | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Team">Team Members</a></li> |
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Collaborations">Collaborations</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Collaborations">Collaborations</a></li> | ||
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Attributions">Attributions</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Attributions">Attributions</a></li> | ||
| Line 215: | Line 213: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-lg-12"> | <div class="col-lg-12"> | ||
| − | <h2 class="pageTitle">Parts Overview | + | <h2 class="pageTitle">Parts Overview</h2> |
| − | + | ||
</div> | </div> | ||
</div> | </div> | ||
| Line 225: | Line 222: | ||
<div class="container content"> | <div class="container content"> | ||
<div class="row"> | <div class="row"> | ||
| − | |||
<div class="col-md-12 content-text"> | <div class="col-md-12 content-text"> | ||
<div class="about-logo"> | <div class="about-logo"> | ||
| − | + | <p>As mentioned in the project description and result, our three different circuits in three different kinds of bacteria well supported our project and each of them contributes a lot to the biobricks. This year, we designed, constructed and verified 25 parts, and 21 of them were submitted. There are <a href="https://2018.igem.org/Team:HUST-China/Composite_part" style="color:blue;">composite parts</a> producing enzyme to transform different carbon source to lactate and transform out of the bacteria. We completely collected the parts from our project and well documented them with our test data.</p> | |
| − | <p | + | |
| − | This year, we designed, constructed and verified 25 parts, and 21 of them were submitted. There are | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div class="col-md- | + | <div class="col-md-10 col-md-offset-1 table-responsive"> |
<table class="table table-bordered table-hover" style="text-align: center;"> | <table class="table table-bordered table-hover" style="text-align: center;"> | ||
<tr> | <tr> | ||
| Line 245: | Line 235: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533000</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533000" style="color:blue;">BBa_K2533000</a></td> |
<td>RBS-ldhD-RBS-lldP</td> | <td>RBS-ldhD-RBS-lldP</td> | ||
| − | <td> | + | <td>ldhD can produce lactate, and lldP can release it from cytoplasm to the environment</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533002</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533001" style="color:blue;">BBa_K2533001</td> |
| + | <td>RBS-ldhDC-RBS-lldP</td> | ||
| + | <td>ldhDC can produce lactate, lldP can release it from cytoplasm to the environment</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533002" style="color:blue;">BBa_K2533002</a></td> | ||
<td>RBS-ldhDnARSdR-RBS-lldP</td> | <td>RBS-ldhDnARSdR-RBS-lldP</td> | ||
<td>ldhDnARSdR can produce lactate, and lldP can release it from cytoplasm to the environment.</td> | <td>ldhDnARSdR can produce lactate, and lldP can release it from cytoplasm to the environment.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533003</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533003" style="color:blue;">BBa_K2533003</a></td> |
<td>RBS-ldhDARSdR-RBS-lldP</td> | <td>RBS-ldhDARSdR-RBS-lldP</td> | ||
<td>ldhDARSdR can produce lactate, and lldP can release it from cytoplasm to the environment.</td> | <td>ldhDARSdR can produce lactate, and lldP can release it from cytoplasm to the environment.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533014</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533014" style="color:blue;">BBa_K2533014</a></td> |
<td>RBS-TH-RBS-gldA-RBS-LLDP</td> | <td>RBS-TH-RBS-gldA-RBS-LLDP</td> | ||
<td>produce and release lactate</td> | <td>produce and release lactate</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533016" style="color:blue;">BBa_K2533016</a></td> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<td>RBS-ldhD-FLAG-lldP-his tag</td> | <td>RBS-ldhD-FLAG-lldP-his tag</td> | ||
<td>ldhD can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | <td>ldhD can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533017</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533017" style="color:blue;">BBa_K2533017</a></td> |
<td>RBS-ldhDC-FLAG-lldP-his tag</td> | <td>RBS-ldhDC-FLAG-lldP-his tag</td> | ||
<td>ldhDC can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | <td>ldhDC can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533018</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533018" style="color:blue;">BBa_K2533018</a></td> |
<td>RBS-ldhDnARSdR-FLAG-RBS-lldP-his tag</td> | <td>RBS-ldhDnARSdR-FLAG-RBS-lldP-his tag</td> | ||
<td>ldhDnARSdR can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | <td>ldhDnARSdR can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533019</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533019" style="color:blue;">BBa_K2533019</a></td> |
<td>RBS-ldhDARSdR-FLAG-RBS-lldP-his tag</td> | <td>RBS-ldhDARSdR-FLAG-RBS-lldP-his tag</td> | ||
<td>ldhDARSdR can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | <td>ldhDARSdR can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533030</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533030" style="color:blue;">BBa_K2533030</a></td> |
<td>RBS-dld</td> | <td>RBS-dld</td> | ||
<td>It encodes D-lactate dehydrogenase.</td> | <td>It encodes D-lactate dehydrogenase.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533031</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533031" style="color:blue;">BBa_K2533031</a></td> |
<td>RBS-lldEFG</td> | <td>RBS-lldEFG</td> | ||
<td>produce L-lactate dehydrogenase complex protein.</td> | <td>produce L-lactate dehydrogenase complex protein.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533032</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533032" style="color:blue;">BBa_K2533032</a></td> |
<td>RBS-dld-TT-Ptac-RBS-lldEFG</td> | <td>RBS-dld-TT-Ptac-RBS-lldEFG</td> | ||
<td>It encodes FAD-dependent D-lactate dehydrogenase and L-lactate dehydrogenase complex protein.</td> | <td>It encodes FAD-dependent D-lactate dehydrogenase and L-lactate dehydrogenase complex protein.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533033</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533033" style="color:blue;">BBa_K2533033</a></td> |
<td>RBS-gapA</td> | <td>RBS-gapA</td> | ||
<td>It encodes glyceraldehyde-3-phosphate dehydrogenase.</td> | <td>It encodes glyceraldehyde-3-phosphate dehydrogenase.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533034</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533034" style="color:blue;">BBa_K2533034</a></td> |
<td>RBS-mdh</td> | <td>RBS-mdh</td> | ||
<td>It encodes NAD dependent malate dehydrogenase.</td> | <td>It encodes NAD dependent malate dehydrogenase.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533035</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533035" style="color:blue;">BBa_K2533035</a></td> |
<td>RBS-gapA-RBS-mdh-TT</td> | <td>RBS-gapA-RBS-mdh-TT</td> | ||
<td>It encodes glyceraldehyde-3-phosphate dehydrogenase and NAD dependent malate dehydrogenase.</td> | <td>It encodes glyceraldehyde-3-phosphate dehydrogenase and NAD dependent malate dehydrogenase.</td> | ||
| Line 322: | Line 312: | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533036</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533036" style="color:blue;">BBa_K2533036</a></td> |
<td>RBS-pflB</td> | <td>RBS-pflB</td> | ||
<td>It encodes pyruvate formate-lyase.</td> | <td>It encodes pyruvate formate-lyase.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533037</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533037" style="color:blue;">BBa_K2533037</a></td> |
<td>RBS-fdh</td> | <td>RBS-fdh</td> | ||
<td>It encodes formate dehydrogenase cytochrome b.</td> | <td>It encodes formate dehydrogenase cytochrome b.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533038</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533038" style="color:blue;">BBa_K2533038</a></td> |
<td>RBS-pflB-RBS-fdh</td> | <td>RBS-pflB-RBS-fdh</td> | ||
<td>It encodes pyruvate formate-lyase and formate.</td> | <td>It encodes pyruvate formate-lyase and formate.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533039</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533039" style="color:blue;">BBa_K2533039</a></td> |
<td>RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh</td> | <td>RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh</td> | ||
<td>It encodes glyceraldehyde-3-phosphate dehydrogenase, NAD dependent malate dehydrogenase, pyruvate formate-lyase and formate dehydrogenase cytochrome b.</td> | <td>It encodes glyceraldehyde-3-phosphate dehydrogenase, NAD dependent malate dehydrogenase, pyruvate formate-lyase and formate dehydrogenase cytochrome b.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533045</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533045" style="color:blue;">BBa_K2533045</a></td> |
<td>RBS-mleS</td> | <td>RBS-mleS</td> | ||
<td>It encodes malate dehydrogenase.</td> | <td>It encodes malate dehydrogenase.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533046</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533046" style="color:blue;">BBa_K2533046</a></td> |
<td>RBS-lldP</td> | <td>RBS-lldP</td> | ||
<td>It encodes L-lactate permease.</td> | <td>It encodes L-lactate permease.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533047</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533047" style="color:blue;">BBa_K2533047</a></td> |
<td>RBS-ldhA</td> | <td>RBS-ldhA</td> | ||
<td>It encodes D-lactate dehydrogenase.</td> | <td>It encodes D-lactate dehydrogenase.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533048</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533048" style="color:blue;">BBa_K2533048</a></td> |
<td>RBS-mleS-RBS-lldP</td> | <td>RBS-mleS-RBS-lldP</td> | ||
<td>It encodes malate dehydrogenase & L-lactate permease.</td> | <td>It encodes malate dehydrogenase & L-lactate permease.</td> | ||
| Line 363: | Line 353: | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533049</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533049" style="color:blue;">BBa_K2533049</a></td> |
<td>RBS-lldP-RBS-ldhA</td> | <td>RBS-lldP-RBS-ldhA</td> | ||
<td>It encodes L-lactate permease & D-lactate dehydrogenase.</td> | <td>It encodes L-lactate permease & D-lactate dehydrogenase.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533050</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533050" style="color:blue;">BBa_K2533050</a></td> |
<td>mleS-lldp-ldhA</td> | <td>mleS-lldp-ldhA</td> | ||
<td>It encodes malate dehydrogenase & L-lactate permease & D-lactate dehydrogenase.</td> | <td>It encodes malate dehydrogenase & L-lactate permease & D-lactate dehydrogenase.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533051</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533051" style="color:blue;">BBa_K2533051</a></td> |
<td>mleS-lldP-TT</td> | <td>mleS-lldP-TT</td> | ||
<td>It encodes malate dehydrogenase & L-lactate permease.</td> | <td>It encodes malate dehydrogenase & L-lactate permease.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533052</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533052" style="color:blue;">BBa_K2533052</a></td> |
<td>RBS-lldP-RBS-ldhA-TT</td> | <td>RBS-lldP-RBS-ldhA-TT</td> | ||
<td>Product and transport of lactic acid.</td> | <td>Product and transport of lactic acid.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533053</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533053" style="color:blue;">BBa_K2533053</a></td> |
<td>RBS-mleS-RBS-lldP-RBS-ldhA-TT</td> | <td>RBS-mleS-RBS-lldP-RBS-ldhA-TT</td> | ||
<td>Product and transport of lactic acid.</td> | <td>Product and transport of lactic acid.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533054</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533054" style="color:blue;">BBa_K2533054</a></td> |
<td>RBS-ldhA-RBS-lldP</td> | <td>RBS-ldhA-RBS-lldP</td> | ||
<td>Product and transport of lactic acid.</td> | <td>Product and transport of lactic acid.</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td>BBa_K2533055</td> | + | <td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2533055" style="color:blue;">BBa_K2533055</a></td> |
<td>RBS-ldhA-RBS-lldP-TT</td> | <td>RBS-ldhA-RBS-lldP-TT</td> | ||
<td>Product and transport of lactic acid.</td> | <td>Product and transport of lactic acid.</td> | ||
Latest revision as of 23:08, 17 October 2018
Parts Overview
As mentioned in the project description and result, our three different circuits in three different kinds of bacteria well supported our project and each of them contributes a lot to the biobricks. This year, we designed, constructed and verified 25 parts, and 21 of them were submitted. There are composite parts producing enzyme to transform different carbon source to lactate and transform out of the bacteria. We completely collected the parts from our project and well documented them with our test data.
| Part Number | Name | Description |
|---|---|---|
| BBa_K2533000 | RBS-ldhD-RBS-lldP | ldhD can produce lactate, and lldP can release it from cytoplasm to the environment |
| BBa_K2533001 | RBS-ldhDC-RBS-lldP | ldhDC can produce lactate, lldP can release it from cytoplasm to the environment |
| BBa_K2533002 | RBS-ldhDnARSdR-RBS-lldP | ldhDnARSdR can produce lactate, and lldP can release it from cytoplasm to the environment. |
| BBa_K2533003 | RBS-ldhDARSdR-RBS-lldP | ldhDARSdR can produce lactate, and lldP can release it from cytoplasm to the environment. |
| BBa_K2533014 | RBS-TH-RBS-gldA-RBS-LLDP | produce and release lactate |
| BBa_K2533016 | RBS-ldhD-FLAG-lldP-his tag | ldhD can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein. |
| BBa_K2533017 | RBS-ldhDC-FLAG-lldP-his tag | ldhDC can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein. |
| BBa_K2533018 | RBS-ldhDnARSdR-FLAG-RBS-lldP-his tag | ldhDnARSdR can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein. |
| BBa_K2533019 | RBS-ldhDARSdR-FLAG-RBS-lldP-his tag | ldhDARSdR can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein. |
| BBa_K2533030 | RBS-dld | It encodes D-lactate dehydrogenase. |
| BBa_K2533031 | RBS-lldEFG | produce L-lactate dehydrogenase complex protein. |
| BBa_K2533032 | RBS-dld-TT-Ptac-RBS-lldEFG | It encodes FAD-dependent D-lactate dehydrogenase and L-lactate dehydrogenase complex protein. |
| BBa_K2533033 | RBS-gapA | It encodes glyceraldehyde-3-phosphate dehydrogenase. |
| BBa_K2533034 | RBS-mdh | It encodes NAD dependent malate dehydrogenase. |
| BBa_K2533035 | RBS-gapA-RBS-mdh-TT | It encodes glyceraldehyde-3-phosphate dehydrogenase and NAD dependent malate dehydrogenase. |
| BBa_K2533036 | RBS-pflB | It encodes pyruvate formate-lyase. |
| BBa_K2533037 | RBS-fdh | It encodes formate dehydrogenase cytochrome b. |
| BBa_K2533038 | RBS-pflB-RBS-fdh | It encodes pyruvate formate-lyase and formate. |
| BBa_K2533039 | RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh | It encodes glyceraldehyde-3-phosphate dehydrogenase, NAD dependent malate dehydrogenase, pyruvate formate-lyase and formate dehydrogenase cytochrome b. |
| BBa_K2533045 | RBS-mleS | It encodes malate dehydrogenase. |
| BBa_K2533046 | RBS-lldP | It encodes L-lactate permease. |
| BBa_K2533047 | RBS-ldhA | It encodes D-lactate dehydrogenase. |
| BBa_K2533048 | RBS-mleS-RBS-lldP | It encodes malate dehydrogenase & L-lactate permease. |
| BBa_K2533049 | RBS-lldP-RBS-ldhA | It encodes L-lactate permease & D-lactate dehydrogenase. |
| BBa_K2533050 | mleS-lldp-ldhA | It encodes malate dehydrogenase & L-lactate permease & D-lactate dehydrogenase. |
| BBa_K2533051 | mleS-lldP-TT | It encodes malate dehydrogenase & L-lactate permease. |
| BBa_K2533052 | RBS-lldP-RBS-ldhA-TT | Product and transport of lactic acid. |
| BBa_K2533053 | RBS-mleS-RBS-lldP-RBS-ldhA-TT | Product and transport of lactic acid. |
| BBa_K2533054 | RBS-ldhA-RBS-lldP | Product and transport of lactic acid. |
| BBa_K2533055 | RBS-ldhA-RBS-lldP-TT | Product and transport of lactic acid. |
