Difference between revisions of "Team:Bulgaria/CONTRIBUTION"

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<div class="contentHolder" id="topTitle">
 
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<a id="pagetitle">PROJECT / CONTRIBUTION</a>
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<a id="pagetitle">PROJECT CONTRIBUTION</a>
 
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<div class="contentHolder" id="PROJECTCONTRIBUTION">
 
<div class="contentHolder" id="PROJECTCONTRIBUTION">
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<h1>TITLE ONE</h1>
 
 
<p>
 
<p>
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PARAGRAPH
+
In the initial stages of our work, we generated and tested the expression of 5
 +
chromoprotein constructs to try our protocols. All of them were cloned into a pSB1C3
 +
vector with a strong promoter and a potent RBS site. Under these conditions, most of the
 +
proteins showed as a severe metabolic burden to the host cells, leading to small sizes of
 +
the colonies and color loss upon re-cultivation. The exception was the amilGFP protein -
 +
was stable enough to be used under the control of a strong promoter and on a high copy
 +
number vector. We also measured its growth kinetics in comparison with the standard
 +
pSB1C3 vector with a red color device. All data could be viewed at the corresponding page
 +
of the registry.
 
</p>
 
</p>
 
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       <a href="https://2018.igem.org/Team:Bulgaria/theproject" >DESCRIPTION</a>
 
       <a href="https://2018.igem.org/Team:Bulgaria/theproject" >DESCRIPTION</a>
 
       <a href="https://2018.igem.org/Team:Bulgaria/DESIGN">DESIGN</a>
 
       <a href="https://2018.igem.org/Team:Bulgaria/DESIGN">DESIGN</a>
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  <a href="https://2018.igem.org/Team:Bulgaria/EXPERIMENTS">EXPERIMENTS</a>
 
 
  <a href="https://2018.igem.org/Team:Bulgaria/PROTOCOLS">PROTOCOLS</a>
 
  <a href="https://2018.igem.org/Team:Bulgaria/PROTOCOLS">PROTOCOLS</a>
 
  <a href="https://2018.igem.org/Team:Bulgaria/LABBOOK">LAB BOOK</a>
 
  <a href="https://2018.igem.org/Team:Bulgaria/LABBOOK">LAB BOOK</a>
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       <a href="https://2018.igem.org/Team:Bulgaria/MEDALSILVER">SILVER</a>
 
       <a href="https://2018.igem.org/Team:Bulgaria/MEDALSILVER">SILVER</a>
 
  <a href="https://2018.igem.org/Team:Bulgaria/MEDALGOLD">GOLD</a>
 
  <a href="https://2018.igem.org/Team:Bulgaria/MEDALGOLD">GOLD</a>
 +
<a href="https://2018.igem.org/Team:Bulgaria/MEDALAWARD">SPECIAL AWARD</a>
 
</div>
 
</div>
 
   </div>  
 
   </div>  
−
   <a href="https://2018.igem.org/Team:Bulgaria/jForm" id="specialMenu">JUDGING FORM</a>
+
   <a href="https://igem.org/2018_Judging_Form?id=2847" id="specialMenu">JUDGING FORM</a>
 
   <a href="javascript:void(0);" class="icon" onclick="burgermenu()">
 
   <a href="javascript:void(0);" class="icon" onclick="burgermenu()">
 
MENU
 
MENU

Latest revision as of 23:27, 17 October 2018

PROJECT USE IS IT SAFE USED ORGANISM STRAINS POTENTIAL RISKS WASTE TREATMENT EMERGENCY REACTIONS PROTECTIVE EQUIPMENT OTHER RULES

In the initial stages of our work, we generated and tested the expression of 5 chromoprotein constructs to try our protocols. All of them were cloned into a pSB1C3 vector with a strong promoter and a potent RBS site. Under these conditions, most of the proteins showed as a severe metabolic burden to the host cells, leading to small sizes of the colonies and color loss upon re-cultivation. The exception was the amilGFP protein - was stable enough to be used under the control of a strong promoter and on a high copy number vector. We also measured its growth kinetics in comparison with the standard pSB1C3 vector with a red color device. All data could be viewed at the corresponding page of the registry.