Difference between revisions of "Team:Bulgaria/safety"

 
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−
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<!-- CUSTOM TOP -->
 
<div class="contentHolder" id="topTitle">
 
<div class="contentHolder" id="topTitle">
−
<a id="pagetitle">PARTS</a>
+
<a id="pagetitle">SAFETY</a>
 
</div>
 
</div>
 
</div>
 
</div>
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<div id="scroldown"></div>
 
<div id="scroldown"></div>
 +
<!-- CUSTOM CONTENT HOLDER -->
 +
<div class="contentHolder" id="WHOUSE">
 +
<h1>WHO WILL USE OUR PRODUCT</h1>
 +
<p>Our target audience consists of scientists and clinicians interested in the detection of the Delta F508 mutation
 +
, responsible for approximately 70% of the Cystic Fibrosis cases in Bulgaria and Europe as a whole. Our system is pla
 +
nned to be used only in a controlled environment.
 +
</p>
 +
</div>
 +
 +
<!-- CUSTOM CONTENT HOLDER -->
 +
<div class="contentHolder" id="SAFE">
 +
<h1>IS IT SAFE?</h1>
 +
<p>Not only is it safer than any other technology that does the same thing,
 +
but it is also drastically cheaper and vastly beneficial due to its simplicity
 +
-  it does not require DNA sequencing for the detection step.
 +
</p>
 +
</div>
  
 
<!-- CUSTOM CONTENT HOLDER -->
 
<!-- CUSTOM CONTENT HOLDER -->
−
<div class="contentHolder" id="PARTS">
+
<div class="contentHolder" id="USEDSTRAINS">
−
<h1>This year, iGEM Bulgaria has submitted three new parts.</h1>
+
<h1>USED ORGANISM STRAINS</h1>
 
<p>
 
<p>
−
<ol class=”contentList”>
+
  # <i>Escherichia coli</i> - DH5&#945; strain
−
<li>Cer sequence from plasmid ColE1 - BBa_K2847000
+
  <br>
−
(a silver medal criteria)
+
  # <i>Escherichia coli</i> - TOP10 strain
−
parts.igem.org/Part:BBa_K2847000</li>
+
</p>
 +
</div>
  
−
<li>Inverted Cer sequence from plasmid ColE1 - BBa_K2847001
+
<!-- CUSTOM CONTENT HOLDER -->
−
parts.igem.org/Part:BBa_K2847001</li>
+
<div class="contentHolder" id="RISKS">
 +
<h1>POTENTIAL RISKS</h1>
 +
<p>Our project poses the typical risks of working with a level 1 GMO.
 +
</p>
 +
</div>
  
−
<li>pSC101 ori + RepA (thermosensitive mutant) + AmpR gene - BBa_K2847002
+
<!-- CUSTOM CONTENT HOLDER -->
−
parts.igem.org/Part:BBa_K2847002</li>
+
<div class="contentHolder" id="WASTE">
−
</ol>
+
<h1>WASTE TREATMENT</h1>
 +
<p>All biological materials and equipment used for handling were collected separately and autoclaved. 
 
</p>
 
</p>
 
</div>
 
</div>
  
  
 +
<!-- CUSTOM CONTENT HOLDER -->
 +
<div class="contentHolder" id="EMERGENCY">
 +
<h1>EMERGENCY REACTIONS</h1>
 +
<p>
 +
 +
<strong>If lab coats or clothes start burning:</strong><br>
 +
emergency showers
 +
<br><strong>If acids or other harmful liquids get in the eyes:</strong><br>
 +
eye showers
 +
<br><strong>If injuries occur:</strong><br>
 +
treat them with first aid-kits, report the injury, go to the hospital if necessary
 +
<br><strong>If solutions with GMO are running down the bench:</strong><br>
 +
swap and disinfect the place with NaClO (bleach)
 +
</p>
 +
</div>
 +
 +
 +
 +
<!-- CUSTOM CONTENT HOLDER -->
 +
<div class="contentHolder" id="EQUIPMENT">
 +
<h1>PROTECTIVE EQUIPMENT</h1>
 +
<p>
 +
<strong>Eye protection</strong><br>
 +
<strong>Lab coats </strong><br>
 +
<strong>Safety glasses in special areas of the lab</strong><br>
 +
<a style="font-size: 15px;">(should be used in other areas if necessary)</a>
 +
</p>
 +
</div>
 +
 +
 +
<!-- CUSTOM CONTENT HOLDER -->
 +
<div class="contentHolder" id="OTHERS">
 +
<h1>OTHER RULES</h1>
 +
<p>
 +
<strong>Do not work alone!</strong><br>
 +
<a style="font-size: 15px;">There should be at least one person in the lab with you.</a><br>
 +
<br><strong>Do not pipette with your mouth!</strong><br>
 +
<br><strong>Disinfect hands after contamination with GMOs!</strong><br>
 +
<br><strong>Disinfect hands and wash them after working in the lab!</strong><br>
 +
<br><strong>Do not eat, swallow, wash and snore with any chemicals!</strong><br>
 +
<br><strong>Do not bring food, drinks, pets and snacks in the lab!</strong><br>
 +
<br><strong>Be sure that you have informed the instructor of the next step of the experiment plan and gotten the permission of him!</strong><br>
 +
<br><strong>Record the general nature of the work clearly in your lab journal!</strong><br>
 +
 +
</p>
 +
</div>
  
 
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−
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+
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 +
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 +
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 +
  <a href="https://2018.igem.org/Team:Bulgaria/survey">SURVEY</a>
 +
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 +
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 +
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 +
      <a href="https://2018.igem.org/Team:Bulgaria/COLLABSURVEYS">SURVEYS</a>
 +
  <a href="https://2018.igem.org/Team:Bulgaria/COLLABOTHERS">OTHERS</a>
 +
  <a href="https://2018.igem.org/Team:Bulgaria/COLLABfollow">#FollowMeToiGEM</a>
 +
</div>
 +
  </div>
 +
  <a href="https://2018.igem.org/Team:Bulgaria/parts">PARTS</a>
 +
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 +
    
 +
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 +
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 +
      <a href="https://2018.igem.org/Team:Bulgaria/MEDALSILVER">SILVER</a>
 +
  <a href="https://2018.igem.org/Team:Bulgaria/MEDALGOLD">GOLD</a>
 +
<a href="https://2018.igem.org/Team:Bulgaria/MEDALAWARD">SPECIAL AWARD</a>
 +
</div>
 +
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 +
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MENU
 
MENU

Latest revision as of 23:31, 17 October 2018

PROJECT USE IS IT SAFE USED ORGANISM STRAINS POTENTIAL RISKS WASTE TREATMENT EMERGENCY REACTIONS PROTECTIVE EQUIPMENT OTHER RULES

WHO WILL USE OUR PRODUCT

Our target audience consists of scientists and clinicians interested in the detection of the Delta F508 mutation , responsible for approximately 70% of the Cystic Fibrosis cases in Bulgaria and Europe as a whole. Our system is pla nned to be used only in a controlled environment.

IS IT SAFE?

Not only is it safer than any other technology that does the same thing, but it is also drastically cheaper and vastly beneficial due to its simplicity - it does not require DNA sequencing for the detection step.

USED ORGANISM STRAINS

# Escherichia coli - DH5α strain
# Escherichia coli - TOP10 strain

POTENTIAL RISKS

Our project poses the typical risks of working with a level 1 GMO.

WASTE TREATMENT

All biological materials and equipment used for handling were collected separately and autoclaved.

EMERGENCY REACTIONS

If lab coats or clothes start burning:
emergency showers
If acids or other harmful liquids get in the eyes:
eye showers
If injuries occur:
treat them with first aid-kits, report the injury, go to the hospital if necessary
If solutions with GMO are running down the bench:
swap and disinfect the place with NaClO (bleach)

PROTECTIVE EQUIPMENT

Eye protection
Lab coats
Safety glasses in special areas of the lab
(should be used in other areas if necessary)

OTHER RULES

Do not work alone!
There should be at least one person in the lab with you.

Do not pipette with your mouth!

Disinfect hands after contamination with GMOs!

Disinfect hands and wash them after working in the lab!

Do not eat, swallow, wash and snore with any chemicals!

Do not bring food, drinks, pets and snacks in the lab!

Be sure that you have informed the instructor of the next step of the experiment plan and gotten the permission of him!

Record the general nature of the work clearly in your lab journal!