Difference between revisions of "Team:SBS SH 112144"

 
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  <span style="font-size:48px; display:block; color:#222; padding-top:70px;" >OUR THEME</span>
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  <p class="widthall" style=" color:#959595; font-size:24px; text-align:left;">CyanoElimination: <b>The lysis of Cyanobacteria</b> in freshwater ecosystem using Cyanophage lysozyme and its <b>commercial implications.</b></p>
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<h1> Welcome to iGEM 2018! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h3>Project Description</h3>
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<p>Cyanobacteria release microcystin LR(MCLR), the most common of microcystin(MC), which is toxic to human and can lead to severe diseases such as liver cancer(Shimizu 2011). Massive reproduction of cyanobacteria has occurred throughout the world and caused serious water quality problem, such as the case of Taihu Lake in 2007. MCLR are structurally stable to withstand physicochemical and biological stresses, including pH, temperature, sunlight and common enzymes, under natural conditions (Harada 1996; Okano et al. 2006). Neither can common water filtering process successfully get rid of it, meaning that only those natural MC-degrading bacterias is capable of degrading MCLR. Studying the degradation process is thus critical to address the water quality problem brought by MCLR. Among 10-more species of such bacteria, our group intends to research on the strand Sphingomonas sp, which possess a MC-degrading pathway that involves 3 genes: mrl A mrl B mrl C that works in a sequential chain reaction(Shimizu 2011) and an additional gene, mrl D, whose product is speculated to aid the transport of MCLR or its degradation process(Bourne, 2001). The MRL A enzyme first linearize the ring-form MCLR, then MRLB cut it into Tetrapeptide, following by the further hydrolysis of Tetra-Peptide into smaller peptides, such as Adda, illustrated by the graph below.(Bourne, 2001). Previous studies have also shown that MCLR can induce the expression of all 3 genes, while linearized MCLR,tetrapeptide and Adda only stimulate the expression of mlr A and mlrB (Shimizu 2011). Being familiar with these information, our group aims to explore on how different combination of these genes can affect the MCLR degradation rates, so that a more efficient degrading model mabe be established.</p>
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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  <span style="font-size:36px; display:block; color:#222; padding-top:50px;padding-bottom:20px;" >Let us introduce you to....</span>
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  <p class="pcss"><b>The rampant growth of cyanobacteria </b>in freshwater ecosystem has become more than an environmental issue. Their incredible ability to multiply and voracious consumption of oxygen often make them a disturbing factor to natural systems. We realized during public outreach that although effective ways to gather and salvage cyanobacteria have been developed, there are barely any success in decomposing these bacteria.</p>
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  <p class="pcss"><b>Our team identified a cyanophagelysozyme, cp-OS lysozyme 1. </b>Alone with other chemicals such as bugbuster, this lysozyme in small reaction systems could lyse the cyanobacteria effectively. Through a series of experiments, we were able to acquire the recombinant protein from E. coli cells. Our modeling helped us to find the best experimental conditions for a prototype device which would contribute to a system of cyanobacteria elimination. Our research lays foundation for the utilization of cyanobacteria components in agricultural, bioenergetic, and even medical fields.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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  <p class="pcss" style="margin:0px 0px 20px 0px;"><nobr><center>Here we presents you with a minecraft video on the introduction to our project.</center></nobr></p>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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Latest revision as of 23:32, 17 October 2018

Header

OUR
CYANOBACTERIA-ELIMINATION
PROJECT

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Home
OUR THEME

CyanoElimination: The lysis of Cyanobacteria in freshwater ecosystem using Cyanophage lysozyme and its commercial implications.

Let us introduce you to....

The rampant growth of cyanobacteria in freshwater ecosystem has become more than an environmental issue. Their incredible ability to multiply and voracious consumption of oxygen often make them a disturbing factor to natural systems. We realized during public outreach that although effective ways to gather and salvage cyanobacteria have been developed, there are barely any success in decomposing these bacteria.

Our team identified a cyanophagelysozyme, cp-OS lysozyme 1. Alone with other chemicals such as bugbuster, this lysozyme in small reaction systems could lyse the cyanobacteria effectively. Through a series of experiments, we were able to acquire the recombinant protein from E. coli cells. Our modeling helped us to find the best experimental conditions for a prototype device which would contribute to a system of cyanobacteria elimination. Our research lays foundation for the utilization of cyanobacteria components in agricultural, bioenergetic, and even medical fields.

VIDEO

Here we presents you with a minecraft video on the introduction to our project.