Difference between revisions of "Team:IIT Delhi/Composite Part"

 
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.bgimg-1, .bgimg-2, .bgimg-3, .bgimg-4 {
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.bgimg-1, .bgimg-2, .bgimg-3, .bgimg-5, .bgimg-4 {
 
   position: relative;
 
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   background-attachment: fixed;
 
   background-attachment: fixed;
 
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   background-size: cover;
 
   background-size: cover;
  
 
}
 
}
 
.bgimg-1 {
 
.bgimg-1 {
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   background-image: url("https://static.igem.org/mediawiki/2018/6/65/T--IIT_Delhi--Finally.png");
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   background-image: url("https://static.igem.org/mediawiki/2018/f/ff/T--IIT_Delhi--white.jpeg");
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   min-height: 800px;
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   min-height: 100px;
 
}
 
}
  
 
.bgimg-2 {
 
.bgimg-2 {
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   background-image: url("https://static.igem.org/mediawiki/2018/2/2a/T--IIT_Delhi--Temp-1.png");
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   background-image: url("https://static.igem.org/mediawiki/2018/f/ff/T--IIT_Delhi--white.jpeg");
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   min-height: 600px;
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   min-height: 100px;
 
}
 
}
  
 
.bgimg-3 {
 
.bgimg-3 {
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   background-image: url("https://static.igem.org/mediawiki/2018/7/72/T--IIT_Delhi--B5.png");
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   background-image: url("https://static.igem.org/mediawiki/2018/f/ff/T--IIT_Delhi--white.jpeg");
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   min-height: 600px;
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   min-height: 100px;
 
}
 
}
  
 
.bgimg-4{
 
.bgimg-4{
 
background-image: url("https://static.igem.org/mediawiki/2018/9/90/T--IIT_Delhi--Dark2.png");
 
background-image: url("https://static.igem.org/mediawiki/2018/9/90/T--IIT_Delhi--Dark2.png");
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   min-height: 600px;
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   min-height: 300px;
  
 
}
 
}
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.bgimg-5 {
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  background-image: url("https://static.igem.org/mediawiki/2018/f/ff/T--IIT_Delhi--white.jpeg");
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  min-height: 100px;
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}
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.caption {
 
.caption {
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/* Turn off parallax scrolling for tablets and phones */
 
/* Turn off parallax scrolling for tablets and phones */
 
@media only screen and (max-device-width: 1024px) {
 
@media only screen and (max-device-width: 1024px) {
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     .bgimg-1, .bgimg-2, .bgimg-3, .bgimg-4 {
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     .bgimg-1, .bgimg-2, .bgimg-3, .bgimg-4, .bgimg-5 {
 
         background-attachment: scroll;
 
         background-attachment: scroll;
 
     }
 
     }
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#banner {
 
#banner {
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background: url("https://static.igem.org/mediawiki/2018/c/ce/T--IIT_Delhi--Compo.png") ;
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background: url("https://static.igem.org/mediawiki/2018/f/ff/T--IIT_Delhi--composite_parts.png") ;
  
 
background-position: center;
 
background-position: center;
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</script>
 
</script>
 
<script>
 
<script>
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    window.sr = ScrollReveal({reset: true});
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  window.sr = ScrollReveal({reset: true});
 
     window.sr = ScrollReveal();
 
     window.sr = ScrollReveal();
 
     /*sr.reveal('.img1', {duration: 700});
 
     /*sr.reveal('.img1', {duration: 700});
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     <body>
 
     <body>
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   <div id="globalWrapperdivi" style="margin-top: -6.5%;z-index: 1">           
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   <div id="globalWrapperdivi" style="margin-top: -6.5%;z-index: 1;background-color: white">           
 
          
 
          
 
    
 
    
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     </div>
 
     </div>
 
   </div>
 
   </div>
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<div class="dropdownsakks">
 
         <div class= "borderYtoX">
 
         <div class= "borderYtoX">
−
   <a href="https://2018.igem.org/Team:IIT_Delhi/Results" >Results</a>
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   <a href="javascript:void(0)" >Results</a>
 
     </div>
 
     </div>
  
−
          
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  <div class="dropdownsakks-content"  style="margin-top: 66px;">
 +
      <a href="https://2018.igem.org/Team:IIT_Delhi/Demonstrate">Demonstrate</a>
 +
      <a href="https://2018.igem.org/Team:IIT_Delhi/InterLab">Interlab</a>
 +
     
 +
    </div>
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 +
 
 +
 
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         </div>
 
          
 
          
 
          
 
          
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     </div>
 
     </div>
 
      
 
      
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        <a href="https://2018.igem.org/Team:IIT_Delhi/Results" style="padding-bottom: 15px;padding-top: 15px;" >Results</a>
 
−
       
 
 
     <button class="overlayved-btn" onclick="myFunction2()" style="padding-bottom: 15px;">Parts
 
     <button class="overlayved-btn" onclick="myFunction2()" style="padding-bottom: 15px;">Parts
 
       <i class="fa fa-caret-right"></i>   
 
       <i class="fa fa-caret-right"></i>   
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     <a href="https://2018.igem.org/Team:IIT_Delhi/Basic_Part" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 3px;" >Basic Parts</a>
 
     <a href="https://2018.igem.org/Team:IIT_Delhi/Basic_Part" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 3px;" >Basic Parts</a>
 
     <a href="https://2018.igem.org/Team:IIT_Delhi/Composite_Part" style="font-size: 15px;font-size: 15px; padding: 10px; padding-bottom: 5px; padding-top: 5px;" >Composite Parts</a>
 
     <a href="https://2018.igem.org/Team:IIT_Delhi/Composite_Part" style="font-size: 15px;font-size: 15px; padding: 10px; padding-bottom: 5px; padding-top: 5px;" >Composite Parts</a>
 +
         
 +
    </div>
 +
 
 +
       
 +
    <button class="overlayved-btn" onclick="myFunction6()" style="padding-bottom: 15px;">Results
 +
      <i class="fa fa-caret-right"></i> 
 +
    </button>
 +
   
 +
    <div class="dropdownsakks-container" id="mydropdownsakks6" style= "font-style: italic;">
 +
    <a href="https://2018.igem.org/Team:IIT_Delhi/Demonstrate" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 3px;" >Demonstrate</a>
 +
    <a href="https://2018.igem.org/Team:IIT_Delhi/InterLab" style="font-size: 15px;font-size: 15px; padding: 10px; padding-bottom: 5px; padding-top: 5px;" >Interlab</a>
 
            
 
            
 
     </div>
 
     </div>
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           </div>   
 
           </div>   
 
      
 
      
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</div>
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</div>
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<!-- Banner -->
 
<!-- Banner -->
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</section>
 
</section>
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<div class="bgimg-1">
 
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  <div class="caption">
 
−
    <span class="border" >Suggestions de do is par xD</span>
 
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  </div>
 
−
</div>
 
  
−
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<div style="color: #777;background-color:white;text-align:center;padding:50px 80px;text-align: justify;">
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<div style="color: black;background-color:white;text-align:center;padding:50px 80px;text-align: justify;margin: auto;width: 80%;font-size: 60%;line-height: 1.5;">
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<table>
−
   <p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;"> BBa_K2814003 = pLTL+RBS+sfGFP+TT (reporter circuit)
+
   <tr>
−
     <br>
+
    <th>S. No</th>
−
      </p>
+
    <th>Part Name</th>
−
      <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">This part was formed by ligating hybrid promoter(lac-tet-lac) with the a reporter sequence containing superfolder GFP along with a ssrA degradation tag and a terminator, a RBS preceded this sequence so that the hybrid promoter could be used in tandem. Hence, sfGFP can be induced by IPTG or/and aTc depending upon the repressors acting upon it.</p>
+
    <th>BioBrick</th>
−
   
+
    <th>Description</th>
−
  <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">The pLTL (Lac-Tet-Lac) (BBa_K2814001) is a hybrid promoter. It is composed of the operator and promoter sequences of tac and the -35 to -135 region of the phage lambda pLac promoter. There are multiple benefits of using the pLTL promoter.
+
    <th>Part Type</th>
−
</p>
+
  </tr>
−
   
+
  <tr>
 +
    <td>1</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814003"><span style="font-weight: 400;">BBa_K2814003</span></a></td>
 +
    <td>pLTL+RBS+sfGFP+TT</td>
 +
    <td>sfGFP reporter under pLTL hybrid promoter</td>
 +
     <td>Reporter</td>
 +
  </tr>
 +
  <tr>
 +
    <td>2</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814006"><span style="font-weight: 400;">BBa_K2814006</span></a></td>
 +
    <td>pLTL+RBS+mKate2+TT</td>
 +
    <td>mKate2 reporter under pLTL hybrid promoter</td>
 +
    <td>Reporter</td>
 +
  </tr>
 +
  <tr>
 +
    <td>3</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814016"><span style="font-weight: 400;">BBa_K2814016</span></a></td>
 +
    <td>SN_TP901 RIFFL</td>
 +
    <td>Second Node of Recombinase based Incoherent Feed-Forward Loop</td>
 +
    <td>Composite</td>
 +
  </tr>
 +
  <tr>
 +
    <td>4</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814023"><span style="font-weight: 400;">BBa_K2814023</span></a></td>
 +
    <td>SN_BxbI_RIFFL</td>
 +
    <td>Alternate Second Node of Recombinase based Incoherent Feed-Forward Loop</td>
 +
    <td>Composite</td>
 +
  </tr>
 +
  <tr>
 +
    <td>5</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814020"><span style="font-weight: 400;">BBa_K2814020</span></a></td>
 +
    <td>TN_TP901-1 RIFFL</td>
 +
    <td>Third Node of Recombinase based Incoherent Feed-Forward Loop</td>
 +
    <td>Composite</td>
 +
  </tr>
 +
  <tr>
 +
    <td>6</td>
 +
    <td><a href="http://parts.igem.org/Part:BBa_K2814024"><span style="font-weight: 400;">BBa_K2814024</span></a></td>
 +
    <td>pTet+RBS+GFP+RBS+TetR+TT</td>
 +
    <td>Transcriptional auto-negative feedback loop (Control)</td>
 +
    <td>Composite</td>
 +
  </tr>
 +
</table></div>
  
−
      <ul style="font-size:20px;" >
 
−
     
 
−
      <li style="font-family: 'Lato', sans-serif;font-weight:400;">The hybrid pLTL shows almost complete repression on being repressed and on complete induction (either by IPTG or aTc), the hybrid pLTL promoter shows a 1.4-2 times higher expression.</li>
 
−
      <li style="font-family: 'Lato', sans-serif;font-weight:400;">The hybrid pLTL promoter also permits flexible gene expression because it can be utilized under either or both repression controls (LacI and TetR) simultaneously.
 
−
</li>
 
−
     
 
−
    </ul>
 
  
−
  <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">The RBS used is standard Elowitz RBS and we have use a codon optimized(for E. coli) variant of superfolder GFP along with a ssrA degradation tag in order to speed up the degradation rate of the GFP. More information related to the sfGFP+ssrA degtag and the part sequence can be found on it’s BioBrick page (BBa_K2814007).The terminator used is a standard double terminator composed of rrnb T1 and T7 Te terminators. (BBa_K2814008)
 
  
−
</p>
 
  
−
<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">References</p>
+
     <div style="background-color: white">
−
  <ol style="font-size:20px;" >  <li style="font-family: 'Lato', sans-serif;font-weight:400;">KONSTANTINOVIC M, MAKSIMOVIC V, NIKCEVIC G, GLISIN V. Hybrid PLtl promoter with dual regulation control. DNA and cell biology. 1991 Jun;10(5):389-95.</li> </ol>
+
         <div  style="background-color: #555;color:black;font-size: 35px;text-align: center;font-family: sans-serif;"><h4 style="color: white">Contact us</h4></div>
−
 
+
             <div style="text-align: center;margin-bottom: -136px;"><h4 style="color:black;font-size: 50px;"><u class="fx" style="cursor: pointer;text-align: center">Address</u></h4><p style="text-align: center;margin-bottom: 16px">Undergraduate Laboratory<br>
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+
−
</div>
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−
 
+
−
<div class="bgimg-2">
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−
  <div class="caption">
+
−
     <span class="border" >Daalo kuch bhi isme xD</span>
+
−
  </div>
+
−
</div>
+
−
 
+
−
<div style="position:relative;">
+
−
  <div style="color: #777;background-color:white;text-align:center;padding:50px 80px;text-align: justify;">
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−
 
+
−
  <p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;"> BBa_K2814006 = pLTL+RBS+mKate2+TT (reporter circuit)
+
−
<br>
+
−
      </p>
+
−
 
+
−
 
+
−
<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">A reporter sequence containing hybrid promoter(lac-tet-lac) with bright fluorescent mKate2 gene along with a ssrA degradation tag and a terminator, a RBS preceded this sequence so that the hybrid promoter could be used in tandem with this reporter. Can be induced by IPTG or/and aTc depending upon the repressors acting upon it.
+
−
 
+
−
</p>
+
−
 
+
−
<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">The pLTL (Lac-Tet-Lac) (BBa_K2814001) is a hybrid promoter. It is composed of the operator and promoter sequences of pTetand  pLac promoter. There are multiple benefits of using the pLTL promoter.
+
−
 
+
−
</p>
+
−
 
+
−
<ul style="font-size:20px;" >
+
−
     
+
−
      <li style="font-family: 'Lato', sans-serif;font-weight:400;">The hybrid pLTL shows almost complete repression on being repressed and on complete induction (either by IPTG or aTc), the hybrid pLTL promoter shows a 1.4-2 times higher expression.</li>
+
−
      <li style="font-family: 'Lato', sans-serif;font-weight:400;">The hybrid pLTL promoter also permits flexible gene expression because it can be utilized under either or both repression controls (LacI and TetR) simultaneously.
+
−
 
+
−
</li>
+
−
     
+
−
    </ul>
+
−
 
+
−
<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">The RBS used is standard Elowitz RBS and we have use a codon optimized variant of mKate2 along with a ssrA degradation tag in order to speed up the degradation rate of the mKate2. More information related to the mKate2+ssrA degtag and the part sequence can be found on it’s BioBrick page (BBa_K2814009).The terminator used is a standard double terminator composed of rrnb T1 and T7 Te terminators. (BBa_K2814008)
+
−
 
+
−
</p>
+
−
 
+
−
 
+
−
 
+
−
  </div>
+
−
</div>
+
−
 
+
−
<div class="bgimg-3">
+
−
  <div class="caption">
+
−
    <span class="border">Daalo kuch bhi xD</span>
+
−
  </div>
+
−
</div>
+
−
 
+
−
<div style="position:relative;">
+
−
  <div style="color: #777;background-color:white;text-align:center;padding:50px 80px;text-align: justify;">
+
−
 
+
−
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814016 = SN_TP901 RIFFL
+
−
<br>
+
−
      </p>
+
−
 
+
−
 
+
−
<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">This part constitutes our second node for Recombinase based Incoherent FeedForward Loop (RIFFL). It contains BxbI integrase between attP and attB sites of TP901-1 Integrase, which when flipped can be activated by induction of hybrid promoter.
+
−
 
+
−
 
+
−
</p>
+
−
 
+
−
<br>
+
−
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814023 = SN_BxbI_RIFFL
+
−
<br>
+
−
      </p>
+
−
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814020 = TN_TP901-1 RIFFL
+
−
<br>
+
−
      </p>
+
−
 
+
−
<br>
+
−
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814020 = TN_TP901-1 RIFFL
+
−
<br>
+
−
      </p>
+
−
 
+
−
 
+
−
+
−
 
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  </div>
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</div>
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    <!--/**/-->
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<div style="background-color: white">
+
−
         <div  style="background-color: #555;font-size: 35px;text-align: center;font-family: sans-serif;"><h4 style="color: white">Contact us</h4></div>
+
−
             <div style="text-align: center;margin-bottom: -136px;"><h4 style="font-size: 50px;"><u class="fx" style="cursor: pointer;text-align: center">Address</u></h4><p style="text-align: center;margin-bottom: 16px">Undergraduate Laboratory<br>
+
 
Department of Biotechnology and Biochemical Engineering, IIT Delhi</p>  
 
Department of Biotechnology and Biochemical Engineering, IIT Delhi</p>  
 
             </div>
 
             </div>
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         <div style="margin-left: 75%;height: auto;max-height: inherit;width: auto;max-width: inherit;margin-bottom: 8px;"><i class="fa fa-facebook-official fx" style="font-size:90px;transition: 0.5s;transition-duration: 0.3s;transition-property: transform;margin-bottom: 30px;"></i></div>
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         <div style="margin-left: 75%;height: auto;max-height: inherit;width: auto;max-width: inherit;margin-bottom: 8px;"><a href="https://www.facebook.com/igemiitdelhi/" style="color:black;"><i class="fa fa-facebook-official fx" style="font-size:90px;transition: 0.5s;transition-duration: 0.3s;transition-property: transform;margin-bottom: 30px;"></i></a></div>
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         <div style="height: auto;max-height: inherit;width: auto;max-width: inherit; margin-left: 20%;margin-top: -125.5px;display: block;"><i class="fa fa-linkedin fxln" style="font-size:90px;transition: 0.5s;transition-duration: 0.3s;transition-property: transform;margin-bottom: 30px;"></i></div>
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         <div style="height: auto;max-height: inherit;width: auto;max-width: inherit; margin-left: 20%;margin-top: -125.5px;display: block;"><a href="https://twitter.com/igem_iit_delhi?lang=en" style="color:black;"><i class="fa fa-twitter fxln" style="font-size:90px;transition: 0.5s;transition-duration: 0.3s;transition-property: transform;margin-bottom: 30px;"></i></a></div>
 
              
 
              
−
         <div style="margin-left: 90%;margin-top: -120px;display: block;height: auto;max-height: inherit;width: auto;max-width: inherit;"><i class="fa fa-instagram fx1" style="font-size:90px;transition: 0.5s;transition-duration: 0.3s;transition-property: transform;margin-bottom: 30px;"></i></div>
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         <div style="margin-left: 90%;margin-top: -120px;display: block;height: auto;max-height: inherit;width: auto;max-width: inherit;"><a href="https://www.instagram.com/igemiitd/" style="color:black;"><i class="fa fa-instagram fx1" style="font-size:90px;transition: 0.5s;transition-duration: 0.3s;transition-property: transform;margin-bottom: 30px;"></i></a></div>
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         <div style="margin-left: 4.5%;margin-top: -120px;display: block;height: auto;max-height: inherit;width: auto;max-width: inherit;"><i class="fa fa-envelope fxm" style="font-size:90px;transition: 0.5s;transition-duration: 0.3s;transition-property: transform;margin-bottom: 30px;"></i></div>
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         <div style="margin-left: 4.5%;margin-top: -120px;display: block;height: auto;max-height: inherit;width: auto;max-width: inherit;"><a href="iitd.igem@gmail.com" style="color:black;"><i class="fa fa-envelope fxm" style="font-size:90px;transition: 0.5s;transition-duration: 0.3s;transition-property: transform;margin-bottom: 30px;"></i></a></div>
 
     </div>
 
     </div>
 
         </div>
 
         </div>

Latest revision as of 23:41, 17 October 2018

iGEM IIT Delhi

Notebook
Lab Records Medal Criteria
Team
Members Attributions
Collaboration
Human Practices
Human Practices Safety Public Engagement
Modelling
Parts
Basic Parts Composite Parts
Results
Demonstrate Interlab
Project
Overview Design
S. No Part Name BioBrick Description Part Type
1 BBa_K2814003 pLTL+RBS+sfGFP+TT sfGFP reporter under pLTL hybrid promoter Reporter
2 BBa_K2814006 pLTL+RBS+mKate2+TT mKate2 reporter under pLTL hybrid promoter Reporter
3 BBa_K2814016 SN_TP901 RIFFL Second Node of Recombinase based Incoherent Feed-Forward Loop Composite
4 BBa_K2814023 SN_BxbI_RIFFL Alternate Second Node of Recombinase based Incoherent Feed-Forward Loop Composite
5 BBa_K2814020 TN_TP901-1 RIFFL Third Node of Recombinase based Incoherent Feed-Forward Loop Composite
6 BBa_K2814024 pTet+RBS+GFP+RBS+TetR+TT Transcriptional auto-negative feedback loop (Control) Composite

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