Difference between revisions of "Team:SBS SH 112144/Human Practices"

 
(18 intermediate revisions by 3 users not shown)
Line 1: Line 1:
 
{{SBS_SH_112144}}
 
{{SBS_SH_112144}}
 +
{{:Team:SBS_SH_112144/header4}}
 
<html>
 
<html>
 +
<head>
 +
<style type="text/css">
 +
.center{
 +
width:70%;
 +
margin:0 auto;
 +
text-align:left;
 +
}
 +
</style>
 +
</head>
 +
<body>
 +
<div style="width:100%; margin-bottom:20px"><img src="https://static.igem.org/mediawiki/2018/8/8d/T--SBS_SH_112144--HUMAN.png" style="width:100%;" /></div>
 +
<div class="center">
 +
<h4>Human Practice Overview</h4>
 +
<p>The Human Practice part of our project is of great concern and can be divided into two major components: interaction with the public which would influence our experimental design as well as how our device would change the current social situation. The former component could be further categorized into five different areas: expert interviews, social research, safety device, public outreach, and collaboration. Through all these activities, we attempt to inform people of the cyanobacteria's negative influence on environment as well as society. Meanwhile, we want to prove our product's practical value and its applicability in the real aquatic environment. The public outreach is crucial to us, because it eventually shifts our focus from eradicating the cyanobacteria in the lakes to successfully degrading the salvaged cyanobacteria into usable commercial components. The our comprehensive design and achievements are presented in details later.</p>
 +
<center><img src="https://static.igem.org/mediawiki/2018/3/3a/T--SBS_SH_112144--cyanobacteria22.jpg" width="800"></center>
  
 +
<h4> Integrated Human Practice </h4>
  
<div class="clear"></div>
+
<h5>Field trip at Lake Taihu</h5>
 +
<p>Scientific research need empathy, and our team was shocked to learn through background research that due to the lack of regulation on factories dumping nitrogen and phosphorus waste into the water, the Taihu lake and its complex ecosystem has been threatened by cyanobacteria since the beginning of 21st century. Thus, we visited the Taihu Laboratory for Lake Ecosystem Research affiliated to Chinese Academy of Sciences to learn more about the background information on the current situation of cyanobacteria outbreak in China, governmental control, and practical ways to deal with them. The formal academic discussion with the researchers and the station supervisor was of great help. We learned that the current solutions could not eradicate cyanobacteria completely and backfire secondary problems such as leaving the salvaged cyanobacteria unprocessed. Comparatively, our approach of using the cyanophage lysozyme should be both cost-effective and environmental friendly. Later that day, we also collected water samples from Lake Taihu in order to gain more insights into the problem of cyanobacterial bloom.</p>
  
  
  
<div class="column full_size">
 
  
<h1>Human Practices</h1>
 
<p>
 
At iGEM we believe societal considerations should be upfront and integrated throughout the design and execution of synthetic biology projects. “Human Practices” refers to iGEM teams’ efforts to actively consider how the world affects their work and the work affects the world. Through your Human Practices activities, your team should demonstrate how you have thought carefully and creatively about whether your project is responsible and good for the world. We invite you to explore issues relating (but not limited) to the ethics, safety, security, and sustainability of your project, and to show how this exploration feeds back into your project purpose, design and execution.
 
</p>
 
  
 +
<center><img src="https://static.igem.org/mediawiki/2018/2/2f/T--SBS_SH_112144--cyanobacteria23.jpg" width="500" height="350"></center>
 +
<center><img src="https://static.igem.org/mediawiki/2018/f/f9/T--SBS_SH_112144--cyanobacteria24.jpg" style="padding-left: 20px;width: 516px;"></center>
  
 +
<h5>Interview with Environmental Science and Engineering Prof. Wang from Fudan University</h5>
  
<p>For more information, please see the <a href="https://2018.igem.org/Human_Practices">Human Practices Hub</a>. There you will find:</p>
+
<p>In July 2018, after researching on existing methods about controlling Cyanobacteria in the Taihu Lake, we shifted to evaluate the practical value our product can create. Thus, we contacted Professor Wang from Fudan University.</p>
+
<ul>
+
<li> an <a href="https://2018.igem.org/Human_Practices/Introduction">introduction</a> to Human Practices at iGEM </li>
+
<li>tips on <a href="https://2018.igem.org/Human_Practices/How_to_Succeed">how to succeed</a> including explanations of judging criteria and advice about how to conduct and document your Human Practices work</li>
+
<li>descriptions of <a href="https://2018.igem.org/Human_Practices/Examples">exemplary work</a> to inspire you</li>
+
<li>links to helpful <a href="https://2018.igem.org/Human_Practices/Resources">resources</a></li>
+
<li>And more! </li>
+
</ul>
+
+
  
  
 +
<center><img src="https://static.igem.org/mediawiki/2018/e/e7/T--SBS_SH_112144--cyanobacteria29.jpg" width="500" height="400"></center>
 +
<p>To our surprise, Professor asserted that our product could only work in the lab because we did not consider the more complicated affecting factors in the lake ecosystem. He further suggested that there's already a well- developed method to collect most of the Cyanobacterium in one area, and then salvage them on the shore. However, there's not a mature way to deal with these muddy Cyanobacteria. To bury them or to burn them create secondary pollution to the environment because if the accumulated nitrogen and phosphorus in the salvaged cyanobacteria flow back into the lake, cyanobacteria bloom would come back. </p>
  
 +
<p>The discussion with Prof. Wang did not frustrate us, but it provides a great inspiration to our new focus: why don't we design a model that could lyse the cyanobacteria and convert it into its different components so that we could convert the "waste" into "wealth". And our design could add up to the already existed methods to fully complete a health "cyanobacteria elimination system".</p>
  
<p>On this page, your team should document all of your Human Practices work and activities. You should write about the Human Practices topics you considered in your project, document any activities you conducted to explore these topics (such as engaging with experts and stakeholders), describe why you took a particular approach (including referencing any work you built upon), and explain if and how you integrated takeaways from your Human Practices work back into your project purpose, design and/or execution. </p>
 
 
<p>If your team has gone above and beyond in work related to safety, then you should document this work on your Safety wiki page and provide a description and link on this page. If your team has developed education and public engagement efforts that go beyond a focus on your particular project, and for which would like to nominate your team for the Best Education and Public Engagement Special Prize, you should document this work on your Education and Education wiki page and provide a description and link here. </p>
 
</div>
 
 
<div class="clear"></div>
 
  
+
<h5>Offline research</h5>
<div class="column full_size">
+
<p>We designed the offline research as the preliminary investigation of a more detailed online research. Therefore, this research aims to provide us with a general idea about public's view on cyanobacteria pollution in China. We went to central Wuxi, where the citizens have been suffering from cyanobacteria in the Taihu Lake, to conduct our offline research. We have received response from 91 questionnaires covering different genders and age groups. Based on analysis of these questionnaires, we were able to learn the public understanding of the cyanobacteria problem, such problem's influence on people's daily life, and public willingness to support our program.</p>
<div class="highlight decoration_background">
+
<p>The results and analysis are stated below :</p>
<p>The iGEM judges will review this page to assess whether you have met the Silver and/or Gold medal requirements based on the Integrated Human Practices criteria listed below. If you nominate your team for the <a href="https://2018.igem.org/Judging/Awards">Best Integrated Human Practices Special Prize</a> by filling out the corresponding field in the <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a>, the judges will also review this page to consider your team for that prize.
+
<p>1.    About the age group</p>
</p>
+
<p>The effective number is 91 in total. Among all the interviewees, 28.5 percent of them are high school or primary school students; 31.9 percent of them study in college; and the rest 29.7 percent of them are already working.</p>
</div>
+
</div>
+
  
<div class="clear extra_space"></div>
 
  
  
 +
<center><img src="https://static.igem.org/mediawiki/2018/d/d9/T--SBS_SH_112144--cyanobacteria25.jpg" style="width:800px; padding:15px 0px;"></center>
 +
<p>2.      To what degree are people aware of the cyanobacteria problem</p>
 +
<p>The effective number is 91 in total. Among all these interviewees, 9 percent of them are well informed of the cyanobacteria problem; 68 percent of them are partially informed; 14 percent of them are not quite informed,; and the rest are not informed at all.</p>
  
<div class="column full_size">
+
<center><img src="https://static.igem.org/mediawiki/2018/c/c2/T--SBS_SH_112144--cyanobacteria26.jpg" style="width:800px; padding:15px 0px;"></center>
+
<h3>Silver Medal Criterion #3</h3>
+
<p>Convince the judges you have thought carefully and creatively about whether your work is responsible and good for the world. Document how you have investigated these issues and engaged with your relevant communities, why you chose this approach, and what you have learned. Please note that surveys will not fulfill this criteria unless you follow scientifically valid methods. </p>
+
  
 +
<p>3.    On what aspect does the cyanobacteria problem have an impact</p>
 +
<p>The effective number is 91 in total. Among all these interviewees, 24 of them agree that cyanobacteria may particularly influence the drinking water; 10 of them take the view that cyanobacteria may have an impact on family's daily water usage; 9 claim that it may affect fishery products; 45 of them indicate that it may have an impact on water quality; 16 of them are worried about the aquatic ecosystem and other environmental issues; 13 interviewees agree that it may damage the appearance of the Lake Taihu; 16 support that there might be dreadful smell; and the rest don't think it will influence their life at all.</p>
  
<h3>Gold Medal Criterion #1</h3>
+
<center><img src="https://static.igem.org/mediawiki/2018/4/4f/T--SBS_SH_112144--cyanobacteria28.jpg"  style="width:800px; padding:15px 0px;"></center>
<p>Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the purpose, design and/or execution of your project. Document how your project has changed based upon your human practices work.
+
<p>ps: for this question, people could choose more than one option </p>
</p>
+
</div>
+
  
 +
<h5>Online Research</h5>
 +
<p>The online research is crucial to our social research, as it provides us with the most precise perspective through which we could learn about the public opinion on our project. Unlike our former questionnaires, this time the online questionnaire is more complicated and more inclusive of detailed questions about the outbreak of cyanobacteria, the government's policies toward such problems, current solutions to the outbreak and our special approach. Among all 21 questions, about 60% are general to cyanobacteria, and over 20% are about our project itself. Even though we've spent a lot of time and effort designing the questionnaire, we knew with satisfaction that our effort paid off when we received over 200 responses. </p>
 +
<p>Part of the results presents as follows</p>
 +
<h5>I. The Outbreak of Cyanobacteria in China<h5>
 +
<p>Most people have heard about water pollution and cyanobacteria outbreak passively through TV programs or websites, but a large proportion of them are indifferent towards the issue: about 55% of the interviewees don't feel that their lives are affected by the outbreak of cyanobacteria. When being asked about what they think lead to cyanobacteria outbreak, the interviewees provide various responses: the unregulated disposal of fertilizer containing nitrogen and phosphorus, the loss of biodiversity in the water body, and even a warmer temperature, etc. They provide their opinions on the advantage and disadvantage about the existing methods: for instance, salvaging is of lower expense but not effective enough, while using chemicals is faster but causes severe harm to the ecosystem. Despite all the different opinions, people generally all agree that there is no one perfect solution right now. </p>
  
 +
<h5>II. About the project itself</h5>
 +
<p>It really encourages us that the interviewees are surprisingly supportive of our theme. Nearly all the interviewees think highly of our experimental design, of which about 90% are willing to support us financially. Apart from this, they would also follow our Wechat official account and use products made by the decomposed cyanobacteria as well.</p>
  
<div class="clear extra_space"></div>
+
<h5>Analysis of the online and offline research</h5>
  
 +
<p>After the offline and online research, we gained lots of insights on how to improve our experiment design and increase people's awareness on the problem. First of all, we learned that a large proportion of our citizens have no idea about synthetic biology in general and the problem of cyanobacteria pollution: to help with such problem, we subsequently set up our official wechat account hoping that the articles we post within could help remove such ignorance and indifference. Moreover, people's concern about the environmental cost, effectiveness, efficiency and repeatability of the method pushed us to make our experimental design more suited to fulfill the public's requirement.</p>
  
 +
<h5>A "cyanobacteria-eliminating" system with our device</h5>
  
<div class="column full_size">
+
<p>Through our painstaking experiment, we were able to create a prototype of the device, equipped with multiple environmental factors best suited for the full functioning of the immobilized lysozyme. Even though due to limited time we had, we were not able to fully test the subsequent processing(high speed centrifugation, components' self-precipitation, etc) of the end products including chemicals, buffer, and cyanobacteria components, it is thrilling enough to know that we are able to integrate a cyanobacteria lysis enzyme into a system that would fully lyse the formidable cyanobacteria into its valuable components. The full potential that the integrated device could achieve is up to future study and research to decide.</p>
<h3>Best Integrated Human Practices Special Prize</h3>
+
  
<p>To compete for the Best Integrated Human Practices prize, please describe your work on this page and also fill out the description on the judging form. </p>
+
<figure>
 +
  <center><img src="https://static.igem.org/mediawiki/2018/b/b0/T--SBS_SH_112144--cyanobacteria30.jpg" width="400">
 +
  <p></p>
 +
<figcaption>(Our integrated device: 1. Immobilized enzyme(lysozyme processed nickel beads, cross-linking or entrapment; 2. Pump; 3. Reaction mixture reservior including cyanobacteria)</figcaption>
 +
  </center>
 +
</figure>
 +
<p></p><p></p>
 +
<p>As we have stated before, the problem of cyanobacteria pollution is a complex issue, and existing methods could resolve some, but not all, parts of the problem. Since our device could resolve the particular issue of unprocessed salvaged cyanobacteria and turn them into valuable components and metabolites, we could add our model into the design of a well functioning cyanobacteria- eliminating system. This system, as indicated in the diagram below, is composed of the human regulation on the disposal of chemicals into the water, the addition of diverse fish species to consume the overpopulated cyanobacteria, the plantation of aquatic plants to restrain the nitrogen and phosphorus level in the water, an encircled water body for the entrapment of cyanobacteria, and factories using the final version of our device to lyse the cyanobacteria and provide the products to science labs and commercial companies. We strongly believe that by restraining the source of the problem, eliminating the existing problem, and turning the wasted into the valuable, we could establish a healthy ecosystem for all humanities. </p>
  
<p>How does your project affect society and how does society influence the direction of your project? How might ethical considerations and stakeholder input guide your project purpose and design and the experiments you conduct in the lab? How does this feedback enter into the process of your work all through the iGEM competition? Document a thoughtful and creative approach to exploring these questions and how your project evolved in the process to compete for this award!</p>
+
<center><img src= "https://static.igem.org/mediawiki/2018/b/b7/T--SBS_SH_112144--Cyanobacteria_Elimination_System_Picture.jpg"  width=700 /></center>
<p>You must also delete the message box on the top of this page to be eligible for this prize.</p>
+
  
 
</div>
 
</div>
 +
</body>
 +
</html>

Latest revision as of 23:47, 17 October 2018

Header

Human Practice Overview

The Human Practice part of our project is of great concern and can be divided into two major components: interaction with the public which would influence our experimental design as well as how our device would change the current social situation. The former component could be further categorized into five different areas: expert interviews, social research, safety device, public outreach, and collaboration. Through all these activities, we attempt to inform people of the cyanobacteria's negative influence on environment as well as society. Meanwhile, we want to prove our product's practical value and its applicability in the real aquatic environment. The public outreach is crucial to us, because it eventually shifts our focus from eradicating the cyanobacteria in the lakes to successfully degrading the salvaged cyanobacteria into usable commercial components. The our comprehensive design and achievements are presented in details later.

Integrated Human Practice

Field trip at Lake Taihu

Scientific research need empathy, and our team was shocked to learn through background research that due to the lack of regulation on factories dumping nitrogen and phosphorus waste into the water, the Taihu lake and its complex ecosystem has been threatened by cyanobacteria since the beginning of 21st century. Thus, we visited the Taihu Laboratory for Lake Ecosystem Research affiliated to Chinese Academy of Sciences to learn more about the background information on the current situation of cyanobacteria outbreak in China, governmental control, and practical ways to deal with them. The formal academic discussion with the researchers and the station supervisor was of great help. We learned that the current solutions could not eradicate cyanobacteria completely and backfire secondary problems such as leaving the salvaged cyanobacteria unprocessed. Comparatively, our approach of using the cyanophage lysozyme should be both cost-effective and environmental friendly. Later that day, we also collected water samples from Lake Taihu in order to gain more insights into the problem of cyanobacterial bloom.

Interview with Environmental Science and Engineering Prof. Wang from Fudan University

In July 2018, after researching on existing methods about controlling Cyanobacteria in the Taihu Lake, we shifted to evaluate the practical value our product can create. Thus, we contacted Professor Wang from Fudan University.

To our surprise, Professor asserted that our product could only work in the lab because we did not consider the more complicated affecting factors in the lake ecosystem. He further suggested that there's already a well- developed method to collect most of the Cyanobacterium in one area, and then salvage them on the shore. However, there's not a mature way to deal with these muddy Cyanobacteria. To bury them or to burn them create secondary pollution to the environment because if the accumulated nitrogen and phosphorus in the salvaged cyanobacteria flow back into the lake, cyanobacteria bloom would come back.

The discussion with Prof. Wang did not frustrate us, but it provides a great inspiration to our new focus: why don't we design a model that could lyse the cyanobacteria and convert it into its different components so that we could convert the "waste" into "wealth". And our design could add up to the already existed methods to fully complete a health "cyanobacteria elimination system".

Offline research

We designed the offline research as the preliminary investigation of a more detailed online research. Therefore, this research aims to provide us with a general idea about public's view on cyanobacteria pollution in China. We went to central Wuxi, where the citizens have been suffering from cyanobacteria in the Taihu Lake, to conduct our offline research. We have received response from 91 questionnaires covering different genders and age groups. Based on analysis of these questionnaires, we were able to learn the public understanding of the cyanobacteria problem, such problem's influence on people's daily life, and public willingness to support our program.

The results and analysis are stated below :

1. About the age group

The effective number is 91 in total. Among all the interviewees, 28.5 percent of them are high school or primary school students; 31.9 percent of them study in college; and the rest 29.7 percent of them are already working.

2. To what degree are people aware of the cyanobacteria problem

The effective number is 91 in total. Among all these interviewees, 9 percent of them are well informed of the cyanobacteria problem; 68 percent of them are partially informed; 14 percent of them are not quite informed,; and the rest are not informed at all.

3. On what aspect does the cyanobacteria problem have an impact

The effective number is 91 in total. Among all these interviewees, 24 of them agree that cyanobacteria may particularly influence the drinking water; 10 of them take the view that cyanobacteria may have an impact on family's daily water usage; 9 claim that it may affect fishery products; 45 of them indicate that it may have an impact on water quality; 16 of them are worried about the aquatic ecosystem and other environmental issues; 13 interviewees agree that it may damage the appearance of the Lake Taihu; 16 support that there might be dreadful smell; and the rest don't think it will influence their life at all.

ps: for this question, people could choose more than one option

Online Research

The online research is crucial to our social research, as it provides us with the most precise perspective through which we could learn about the public opinion on our project. Unlike our former questionnaires, this time the online questionnaire is more complicated and more inclusive of detailed questions about the outbreak of cyanobacteria, the government's policies toward such problems, current solutions to the outbreak and our special approach. Among all 21 questions, about 60% are general to cyanobacteria, and over 20% are about our project itself. Even though we've spent a lot of time and effort designing the questionnaire, we knew with satisfaction that our effort paid off when we received over 200 responses.

Part of the results presents as follows

I. The Outbreak of Cyanobacteria in China

Most people have heard about water pollution and cyanobacteria outbreak passively through TV programs or websites, but a large proportion of them are indifferent towards the issue: about 55% of the interviewees don't feel that their lives are affected by the outbreak of cyanobacteria. When being asked about what they think lead to cyanobacteria outbreak, the interviewees provide various responses: the unregulated disposal of fertilizer containing nitrogen and phosphorus, the loss of biodiversity in the water body, and even a warmer temperature, etc. They provide their opinions on the advantage and disadvantage about the existing methods: for instance, salvaging is of lower expense but not effective enough, while using chemicals is faster but causes severe harm to the ecosystem. Despite all the different opinions, people generally all agree that there is no one perfect solution right now.

II. About the project itself

It really encourages us that the interviewees are surprisingly supportive of our theme. Nearly all the interviewees think highly of our experimental design, of which about 90% are willing to support us financially. Apart from this, they would also follow our Wechat official account and use products made by the decomposed cyanobacteria as well.

Analysis of the online and offline research

After the offline and online research, we gained lots of insights on how to improve our experiment design and increase people's awareness on the problem. First of all, we learned that a large proportion of our citizens have no idea about synthetic biology in general and the problem of cyanobacteria pollution: to help with such problem, we subsequently set up our official wechat account hoping that the articles we post within could help remove such ignorance and indifference. Moreover, people's concern about the environmental cost, effectiveness, efficiency and repeatability of the method pushed us to make our experimental design more suited to fulfill the public's requirement.

A "cyanobacteria-eliminating" system with our device

Through our painstaking experiment, we were able to create a prototype of the device, equipped with multiple environmental factors best suited for the full functioning of the immobilized lysozyme. Even though due to limited time we had, we were not able to fully test the subsequent processing(high speed centrifugation, components' self-precipitation, etc) of the end products including chemicals, buffer, and cyanobacteria components, it is thrilling enough to know that we are able to integrate a cyanobacteria lysis enzyme into a system that would fully lyse the formidable cyanobacteria into its valuable components. The full potential that the integrated device could achieve is up to future study and research to decide.

(Our integrated device: 1. Immobilized enzyme(lysozyme processed nickel beads, cross-linking or entrapment; 2. Pump; 3. Reaction mixture reservior including cyanobacteria)

As we have stated before, the problem of cyanobacteria pollution is a complex issue, and existing methods could resolve some, but not all, parts of the problem. Since our device could resolve the particular issue of unprocessed salvaged cyanobacteria and turn them into valuable components and metabolites, we could add our model into the design of a well functioning cyanobacteria- eliminating system. This system, as indicated in the diagram below, is composed of the human regulation on the disposal of chemicals into the water, the addition of diverse fish species to consume the overpopulated cyanobacteria, the plantation of aquatic plants to restrain the nitrogen and phosphorus level in the water, an encircled water body for the entrapment of cyanobacteria, and factories using the final version of our device to lyse the cyanobacteria and provide the products to science labs and commercial companies. We strongly believe that by restraining the source of the problem, eliminating the existing problem, and turning the wasted into the valuable, we could establish a healthy ecosystem for all humanities.