Difference between revisions of "Team:BIT-China/Description"

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{{BIT-China}}
 
 
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            background-color: transparent !important;
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        #content {
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<h1>Description</h1>
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            padding: 0;
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            background-color: transparent !important;
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        }
  
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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        #top_title {
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            display: none;
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        }
  
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</div>
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            border-style: transparent !important;
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    </style>
  
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    <link rel="stylesheet" href="https://2018.igem.org/Template:BIT-China/css/common-style?action=raw&amp;ctype=text/css">
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    <link rel="stylesheet" href="https://2018.igem.org/Template:BIT-China/css/project-common-style?action=raw&amp;ctype=text/css">
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</head>
  
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<div class="column two_thirds_size">
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<body>
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<h3>What should this page contain?</h3>
+
−
<ul>
+
−
<li> A clear and concise description of your project.</li>
+
−
<li>A detailed explanation of why your team chose to work on this particular project.</li>
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−
<li>References and sources to document your research.</li>
+
−
<li>Use illustrations and other visual resources to explain your project.</li>
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</ul>
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</div>
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<div class="column third_size" >
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            <ul id="left-nav">
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<div class="highlight decoration_A_full">
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        <li>
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<h3>Inspiration</h3>
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            <a>PROJECT</a>
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<p>See how other teams have described and presented their projects: </p>
+
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Background">Background</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Description">Description</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Design">Idea & Design</a></li>
 +
            </ul>
 +
        </li>
  
−
<ul>
+
        <li>
−
<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
+
            <a>EXPERIMENTS</a>
−
<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
+
            <ul>
−
<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
+
                <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsRegulator">Regulator</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
+
                <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsFeedback">Feedback</a></li>
−
</ul>
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                <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsOutput">Output</a></li>
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</div>
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                <li><a href="https://2018.igem.org/Team:BIT-China/Results">Results</a></li>
−
</div>
+
            </ul>
 +
        </li>
  
 +
        <li>
 +
            <a>MODELING</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Model">Overview</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/FluorescentProbesModel">Fluorescent Probe Model </a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/H2O2DecompositionModel">H<sub>2</sub>O<sub>2</sub>
 +
                        Decomposition Model</a></li>
  
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/roGFP2-Orp1MichaelisEquationModel">roGFP2-Orp1
 +
                        Michaelis equation Model</a></li>
 +
            </ul>
 +
        </li>
  
 +
        <li>
 +
            <a>HUMAN PRACTICES</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/HPOverview">Overview</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Human_Practices">Integrated Human Practices</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Public_Engagement">Education & Public Engagement</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Collaborations">Collaborations</a></li>
 +
            </ul>
 +
        </li>
  
−
<div class="column two_thirds_size" >
+
        <li>
−
<h3>Advice on writing your Project Description</h3>
+
            <a>NOTEBOOK</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Notebook">Lab Book</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Protocols">Methodology / Protocols</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Equipment">Material & Equipment</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/InterLab">Measurement / InterLab</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Safety">Safety</a></li>
 +
            </ul>
 +
        </li>
  
−
<p>
+
        <li>
−
We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements.  
+
            <a>ACHIEVEMENTS</a>
−
</p>
+
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/JudgingForm">Judging Form</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Parts">Parts</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Improve">Improve</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Applied_Design">Applied Design</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Demonstrate">Demonstrate</a></li>
 +
            </ul>
 +
        </li>
  
−
</div>
+
        <li>
 +
            <a>TEAM</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Team">Members</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Attributions">Attributions</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Gallery">Gallery</a></li>
 +
            </ul>
 +
        </li>
 +
    </ul>
 +
    <a href="https://2018.igem.org/Team:BIT-China"><img id="imgA" class="imgA-new-pos" src="https://static.igem.org/mediawiki/2018/4/46/T--BIT-China--iGEM2018-A_img.png " /></a>
 +
    <!-- end -->
  
−
<div class="column third_size">
+
    <div class="PRO-white-head"></div>
−
<h3>References</h3>
+
−
<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
+
  
−
</div>
+
    <img class="PRO-BG" src="https://static.igem.org/mediawiki/2018/7/74/T--BIT-China--iGEM2018-project-bg.png">
  
 +
    <div id="PRO-content-all" class="content_container" style="margin-top:25vh;">
 +
        <div style="font-family: 'kg_second_chances_solidRg';font-size: 30px;color: #131313;width: 100%;float:right;">
 +
            <a style="border-bottom: 3px solid #131313;color: #131313;text-decoration: none;right: 0;float: right;">DESCRIPTION</a>
 +
        </div>
  
 +
        <div id="PRO1" class="cd-section">
 +
            <div class="PRO-title-2">
 +
                <a style="text-decoration:none;color:#131313;">Overview</a>
 +
            </div>
  
 +
            <div class="PRO-content-all PRO-margin-toTitle2">
 +
                <div class="PRO-content">
 +
                    <p class="PRO-content-p">
 +
                        To achieve our goal of detecting the antioxidants capacity of different products in living
 +
                        cells, it our system can be divided into three parts, regulator, feedback and output.
 +
                    </p>
 +
                </div>
 +
            </div>
 +
        </div>
  
 +
        <div id="PRO2" class="cd-section PRO-margin-Title2Up">
 +
            <div class="PRO-title-2">
 +
                <a style="text-decoration:none;color:#131313;">Regulator</a>
 +
            </div>
  
 +
            <div class="PRO-content-all">
 +
                <div class="PRO-content">
 +
                    <p class="PRO-content-p PRO-margin-toTitle2">
 +
                        Firstly, we choose to increase the intracellular ROS content, considering that:
 +
                    </p>
 +
 +
                    <ul class="PRO-content-p PRO-margin-toContentP">
 +
                        <li>
 +
                            ● The level of ROS in normal cells is low and hard to detection.
 +
                        </li>
 +
                        <li>
 +
                            ● Make the cell under oxidative stress to simulate the normal cellular oxidative process
 +
                            or, rather, the aging process.
 +
                        </li>
 +
                        <li>
 +
                            ● The most intuitive effect of antioxidants is their reduction of intracellular ROS
 +
                            content. We can evaluate antioxidants indirectly through the remaining amount of ROS.
 +
                        </li>
 +
                        <li>
 +
                            ● After reviewing most cell-based antioxidant detection methods recorded in literature, we
 +
                            discover that almost all protocols will induce cell in an oxidant stress firstly.
 +
                        </li>
 +
                    </ul>
 +
 +
                    <p class="PRO-content-p PRO-margin-toContentP">
 +
                        Therefore, we established this part to do such kind of thing. Meanwhile, we hope the way to
 +
                        stimulate the accumulation of ROS is easy and equipment-independent, which makes our system
 +
                        more available for customers.
 +
                    </p>
 +
                    <p class="PRO-content-p PRO-margin-toContentP">
 +
                        However, higher oxidative stress is always accompanied by lower survival rate, in hence we also
 +
                        need to do something to make our host cell more robust and prevent cells from dying for
 +
                        oxidation.
 +
                    </p>
 +
                </div>
 +
            </div>
 +
        </div>
 +
 +
        <div id="PRO2" class="cd-section PRO-margin-Title2Up">
 +
            <div class="PRO-title-2">
 +
                <a style="text-decoration:none;color:#131313;">Feedback</a>
 +
            </div>
 +
 +
            <div class="PRO-content-all">
 +
                <div class="PRO-content">
 +
                    <p class="PRO-content-p PRO-margin-toTitle2">
 +
                        After increasing the concentration of ROS in cell, there's still somethings we need to
 +
                        consider:
 +
                    </p>
 +
 +
                    <ul class="PRO-content-p PRO-margin-toContentP">
 +
                        <li>
 +
                            ● The high level of ROS will damage the cells and even lead to cells death.
 +
                        </li>
 +
                        <li>
 +
                            ● Low survival rate under high oxidant state may affect the signal output of our system,
 +
                            making our results not convincing.
 +
                        </li>
 +
                        <li>
 +
                            ● It's hard to control the ROS accumulation in every assay, which means our test may be
 +
                            NON-repeatable.
 +
                        </li>
 +
                    </ul>
 +
 +
                    <p class="PRO-content-p PRO-margin-toContentP">
 +
                        Based on these reasons, we need to add an feedback system to control the concentration of ROS
 +
                        in vivo, make it higher than normal level, lower than death level to maintain cell viability
 +
                        and last, steady for test. We solve this problem by adding an ROS sensor, which has a suitable
 +
                        sensing threshold so that it can maintain the balancing between the high level of ROS in living
 +
                        cell and survival rate of cells.
 +
                    </p>
 +
                </div>
 +
            </div>
 +
        </div>
 +
 +
        <div id="PRO2" class="cd-section PRO-margin-Title2Up">
 +
            <div class="PRO-title-2">
 +
                <a style="text-decoration:none;color:#131313;">Output</a>
 +
            </div>
 +
 +
            <div class="PRO-content-all">
 +
                <div class="PRO-content">
 +
                    <p class="PRO-content-p PRO-margin-toTitle2">
 +
                        Last but not least, the part of characterizing residual amount of intracellular ROS.
 +
                    </p>
 +
                    <p class="PRO-content-p PRO-margin-toContentP">
 +
                        Different from chemical detecting methods, our signal output substance must be:
 +
                    </p>
 +
 +
                    <ul class="PRO-content-p PRO-margin-toContentP">
 +
                        <li>
 +
                            ● simpler for users to detect.
 +
                        </li>
 +
                        <li>
 +
                            ● harmless to cells.
 +
                        </li>
 +
                        <li>
 +
                            ● accurate and sensitive enough to reflect the intracellular ROS content.
 +
                        </li>
 +
                        <li>
 +
                            ● Reversible so we can get real-time data.
 +
                        </li>
 +
                    </ul>
 +
 +
                    <p class="PRO-content-p PRO-margin-toContentP">
 +
                        To meet those needs, chemical redox probe has been out of our consideration, even though they
 +
                        have characteristic of extreme-high sensitivity, still they are irreversibility and unstable.
 +
                        We prefer a kind of characterization which can exist in living cell also in a consist level for
 +
                        stable measurement.
 +
                    </p>
 +
                </div>
 +
            </div>
 +
        </div>
 +
    </div>
 +
 +
    <!-- footer start -->
 +
    <div class="footer-all" style="margin-top:65px;">
 +
        <div class="footer-left">
 +
            <img src="https://static.igem.org/mediawiki/2018/c/ca/T--BIT-China--iGEM2018-sponsor-1.png">
 +
            <img src="https://static.igem.org/mediawiki/2018/0/07/T--BIT-China--iGEM2018-sponsor-2.png">
 +
        </div>
 +
        <div class="footer-right">
 +
            <p style="font-family:'kg_second_chances_solidRg';font-size:12px;margin: 0;padding: 0 0 3px;margin-top: 8px;">Contact</p>
 +
            <p style="font-size:15px;margin: 0;padding: 0;margin-top: 4px;">Institute of
 +
                Biotransformation and synthetic biosystem School of Chemistry and Chemical Engineering.
 +
            </p>
 +
            <p style="font-size:15px;margin: 0;padding: 0;margin-top: 4px;">
 +
                Beijing Institute of Technology
 +
            </p>
 +
            <p style="font-size:15px;margin: 0;padding: 0;margin-top: 4px;">
 +
                100081, Beijing
 +
            </p>
 +
            <p style="font-size:15px;margin: 0;padding: 0;margin-top: 4px;">
 +
                Email:lichun@bit.edu.cn
 +
            </p>
 +
 +
            <p style="font-size:15px;margin: 0;padding: 0;margin-top: 4px;color:  rgb(161, 161, 161);">
 +
                Copyright © 2018 BIT-China
 +
            </p>
 +
        </div>
 +
    </div>
 +
    <!-- end -->
 +
 +
    <script src="https://2018.igem.org/Template:BIT-China/js/jquery-min?action=raw&ctype=text/javascript"></script>
 +
    <script src="https://2018.igem.org/Template:BIT-China/js/copy-tendina?action=raw&ctype=text/javascript"></script>
 +
    <script>
 +
        $('#left-nav').tendina({
 +
            animate: true,
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</body>
  
 
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Latest revision as of 00:24, 18 October 2018

Overview

To achieve our goal of detecting the antioxidants capacity of different products in living cells, it our system can be divided into three parts, regulator, feedback and output.

Regulator

Firstly, we choose to increase the intracellular ROS content, considering that:

  • ● The level of ROS in normal cells is low and hard to detection.
  • ● Make the cell under oxidative stress to simulate the normal cellular oxidative process or, rather, the aging process.
  • ● The most intuitive effect of antioxidants is their reduction of intracellular ROS content. We can evaluate antioxidants indirectly through the remaining amount of ROS.
  • ● After reviewing most cell-based antioxidant detection methods recorded in literature, we discover that almost all protocols will induce cell in an oxidant stress firstly.

Therefore, we established this part to do such kind of thing. Meanwhile, we hope the way to stimulate the accumulation of ROS is easy and equipment-independent, which makes our system more available for customers.

However, higher oxidative stress is always accompanied by lower survival rate, in hence we also need to do something to make our host cell more robust and prevent cells from dying for oxidation.

Feedback

After increasing the concentration of ROS in cell, there's still somethings we need to consider:

  • ● The high level of ROS will damage the cells and even lead to cells death.
  • ● Low survival rate under high oxidant state may affect the signal output of our system, making our results not convincing.
  • ● It's hard to control the ROS accumulation in every assay, which means our test may be NON-repeatable.

Based on these reasons, we need to add an feedback system to control the concentration of ROS in vivo, make it higher than normal level, lower than death level to maintain cell viability and last, steady for test. We solve this problem by adding an ROS sensor, which has a suitable sensing threshold so that it can maintain the balancing between the high level of ROS in living cell and survival rate of cells.

Output

Last but not least, the part of characterizing residual amount of intracellular ROS.

Different from chemical detecting methods, our signal output substance must be:

  • ● simpler for users to detect.
  • ● harmless to cells.
  • ● accurate and sensitive enough to reflect the intracellular ROS content.
  • ● Reversible so we can get real-time data.

To meet those needs, chemical redox probe has been out of our consideration, even though they have characteristic of extreme-high sensitivity, still they are irreversibility and unstable. We prefer a kind of characterization which can exist in living cell also in a consist level for stable measurement.

Contact

Institute of Biotransformation and synthetic biosystem School of Chemistry and Chemical Engineering.

Beijing Institute of Technology

100081, Beijing

Email:lichun@bit.edu.cn

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