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} | } | ||
</style> | </style> | ||
− | + | ||
</head> | </head> | ||
− | <body | + | <body> |
− | + | <ul id="left-nav"> | |
<li> | <li> | ||
<a>PROJECT</a> | <a>PROJECT</a> | ||
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<li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsFeedback">Feedback</a></li> | <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsFeedback">Feedback</a></li> | ||
<li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsOutput">Output</a></li> | <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsOutput">Output</a></li> | ||
− | <li><a href="https://2018.igem.org/Team:BIT-China/ | + | <li><a href="https://2018.igem.org/Team:BIT-China/Results">Results</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
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<ul> | <ul> | ||
<li><a href="https://2018.igem.org/Team:BIT-China/Model">Overview</a></li> | <li><a href="https://2018.igem.org/Team:BIT-China/Model">Overview</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/FluorescentProbesModel">Fluorescent Probe Model </a></li> | ||
<li><a href="https://2018.igem.org/Team:BIT-China/H2O2DecompositionModel">H<sub>2</sub>O<sub>2</sub> | <li><a href="https://2018.igem.org/Team:BIT-China/H2O2DecompositionModel">H<sub>2</sub>O<sub>2</sub> | ||
Decomposition Model</a></li> | Decomposition Model</a></li> | ||
− | <li><a href="https://2018.igem.org/Team:BIT-China/ | + | |
− | + | <li><a href="https://2018.igem.org/Team:BIT-China/roGFP2-Orp1MichaelisEquationModel">roGFP2-Orp1 | |
+ | Michaelis equation Model</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
<li> | <li> | ||
− | <a> | + | <a>HUMAN PRACTICES</a> |
<ul> | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/HPOverview">Overview</a></li> | ||
<li><a href="https://2018.igem.org/Team:BIT-China/Human_Practices">Integrated Human Practices</a></li> | <li><a href="https://2018.igem.org/Team:BIT-China/Human_Practices">Integrated Human Practices</a></li> | ||
<li><a href="https://2018.igem.org/Team:BIT-China/Public_Engagement">Education & Public Engagement</a></li> | <li><a href="https://2018.igem.org/Team:BIT-China/Public_Engagement">Education & Public Engagement</a></li> | ||
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<div class="ME-title-content"> | <div class="ME-title-content"> | ||
<img class="ME-title-img" src="https://static.igem.org/mediawiki/2018/8/84/T--BIT-China--iGEM2018-top_mask.svg"> | <img class="ME-title-img" src="https://static.igem.org/mediawiki/2018/8/84/T--BIT-China--iGEM2018-top_mask.svg"> | ||
− | <a style="text-decoration: none;color:#131313;"> | + | <a style="text-decoration: none;color:#131313;">WHAT IS THE POINT?</a> |
<img class=" | <img class=" | ||
ME-title-img" src="https://static.igem.org/mediawiki/2018/4/46/T--BIT-China--iGEM2018-lower_mask.svg"> | ME-title-img" src="https://static.igem.org/mediawiki/2018/4/46/T--BIT-China--iGEM2018-lower_mask.svg"> | ||
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<table> | <table> | ||
<colgroup> | <colgroup> | ||
− | <col style="width: | + | <col style="width: 30%;" /> |
− | <col style="width: | + | <col style="width: 30%;" /> |
<col /> | <col /> | ||
− | <col style="width: | + | <col style="width: 40%;" /> |
</colgroup> | </colgroup> | ||
<thead> | <thead> | ||
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<table> | <table> | ||
<colgroup> | <colgroup> | ||
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<col /> | <col /> | ||
− | <col style="width: | + | <col style="width: 40%;" /> |
</colgroup> | </colgroup> | ||
<tbody style="font-size: 16px;"> | <tbody style="font-size: 16px;"> | ||
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<table> | <table> | ||
<colgroup> | <colgroup> | ||
− | <col /> | + | <col style="width: 30%;" /> |
− | <col /> | + | <col style="width: 70%;" /> |
+ | |||
</colgroup> | </colgroup> | ||
<thead> | <thead> | ||
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<table> | <table> | ||
<colgroup> | <colgroup> | ||
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+ | |||
</colgroup> | </colgroup> | ||
<tbody style="font-size: 16px;"> | <tbody style="font-size: 16px;"> | ||
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<h3 id="a-nav-dot-box-M2" class="ME-title-content-a"> | <h3 id="a-nav-dot-box-M2" class="ME-title-content-a"> | ||
<span class="collapseCircle"></span> | <span class="collapseCircle"></span> | ||
− | 2. | + | 2. Marker |
</h3> | </h3> | ||
<div id="collapse_body_M2" class="collapse_body"> | <div id="collapse_body_M2" class="collapse_body"> | ||
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<table> | <table> | ||
<colgroup> | <colgroup> | ||
− | <col /> | + | <col style="width: 50%;" /> |
− | <col /> | + | <col style="width: 50%;" /> |
+ | |||
</colgroup> | </colgroup> | ||
<thead> | <thead> | ||
<tr style="font-size: 20px;"> | <tr style="font-size: 20px;"> | ||
− | <th> | + | <th>Marker</th> |
<th>From</th> | <th>From</th> | ||
</tr> | </tr> | ||
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<table> | <table> | ||
<colgroup> | <colgroup> | ||
− | <col /> | + | <col style="width: 50%;" /> |
− | <col /> | + | <col style="width: 50%;" /> |
+ | |||
</colgroup> | </colgroup> | ||
<tbody style="font-size: 16px;"> | <tbody style="font-size: 16px;"> | ||
<tr> | <tr> | ||
− | <td>Trans 2K DNA | + | <td>Trans 2K DNA Marker</td> |
<td>Trans Gen Biotech</td> | <td>Trans Gen Biotech</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>Trans 2K plus DNA | + | <td>Trans 2K plus DNA Marker</td> |
<td>Trans Gen Biotech</td> | <td>Trans Gen Biotech</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>Trans 1KB DNA | + | <td>Trans 1KB DNA Marker</td> |
<td>Trans Gen Biotech</td> | <td>Trans Gen Biotech</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td>Trans 2K plusⅡDNA | + | <td>Trans 2K plusⅡDNA Marker</td> |
<td>Trans Gen Biotech</td> | <td>Trans Gen Biotech</td> | ||
</tr> | </tr> | ||
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</colgroup> | </colgroup> | ||
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+ | <tr> | ||
+ | <td>Vitamin E</td> | ||
+ | <td> solarbio</td> | ||
+ | </tr> | ||
</tbody> | </tbody> | ||
</table> | </table> | ||
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<tbody style="font-size: 16px;"> | <tbody style="font-size: 16px;"> |
Latest revision as of 00:54, 18 October 2018
Equipments
Name | Type | Company |
---|
Transferpettor | 1000µl 100µl 10µl 2.5µl | Eppendorf |
ELIASA | Elx808 | America Bioteck company |
Electrophresis Apparatus | DYY-6C | Beijing LIUYI bio-technology company |
OD Machine | UV-2100 | UNICO |
Incubator Shaker | IS-RDS3 | Beijing KECHUANGBAIFANG Technical Co., Ltd |
Electro-heating Standing-temperature Water Bath | DK-8D | Shanghai YIHENG Technical Co., Ltd |
Gel-imaging System | GeneGenius | SYNGENE |
Blue Rubber Cutting Machine | BD-BGCI | Biofriend |
Electro-heating Standing-temperature Cultivator | SKP-02.420 | America HENGFENG company |
High Speed Freezing Centrifuge | D-37520 | Germany Thermo company |
Low temperature enzyme even instrument | L1 | HEMA |
Electronic Balance | FA2104SW | MINQIAO |
High-Pressure Steam Sterilization Pot | MLS-3750 | SANYO |
Laminar Flow Cabinet | SW-CJ-2FD | Taisite |
Microwave | / | Galanz |
Electro-heating Standing-temperature Drum Wind Drying Machine | DHG-9030A | Shanghai YIHENG Technical Co., Ltd |
96 Well Thermal Cycler | / | Gene Company Limited |
High Speed Micro-Centrifuge | D3024 | SCILOGEX |
Refrigerator | BCD-610W | SIEMENS |
Heto Ultra Freeze | / | Thermo |
Ultrapure Water System | / | / |
Ice Machine | SPR-120 | SIMAG |
Nano Drop | 2000c | America Thermo Scientific company |
Microscope | / | Ruich Allway |
Noise Isolating Tamber | vcx750 | Ningbo Scientz Biotechnology CO. , Ltd |
Noise Cleaner | KQ-100DE | Kunshang Noise Equipment CO. Ltd. |
Real-time fluorescent quantitative PCR | LightCycler 96 | Roche |
1. Antibiotic
Antibiotic | Preparation method |
---|
Ampicillin | Dissolved in distilled water and the final concentration is 100mg/ml |
Chloromycetin | Dissolved in absolute ethyl alcohol and final concentration is 25 mg/ml |
2. Marker
Marker | From |
---|
Trans 2K DNA Marker | Trans Gen Biotech |
Trans 2K plus DNA Marker | Trans Gen Biotech |
Trans 1KB DNA Marker | Trans Gen Biotech |
Trans 2K plusⅡDNA Marker | Trans Gen Biotech |
3. Buffer
Buffer | From |
---|
6×DNA Loading buffer | Trans Gen Biotech |
2×PrimeSTARCG buffer | TaKaRa |
2×Taq PCR Master Mix | Biomed |
2×ES Taq Master Mix | CWBio |
2×SYBR Green qPCR Master Mix | TaKaPa |
10×T4 DNA Ligase Buffer | TaKaRa |
10×Fast Digest Green Buffer | Scientific |
10×Fast Digest Buffer | Scientific |
10×Pfu Buffer | Biomed |
5×Trans Start FastPfu Buffer | Trans Gen Biotech |
5×PrimeSTAR Buffer | TaKaRa |
5×FastPfu Fly Buffer | Trans Gen Biotech |
5×Primrscript RTMaster Mix | TaKaRa |
4. Enzyme
Enzyme | From |
---|
PrimeSTAR HS DNA polymerase | TaKaRa |
2×SYBR Green qPCR Master Mix | TaKaPa |
5×Primrscript RTMaster Mix | TaKaRa |
Pfu DNA polymerase | Biomed |
Trans Start FastPfu DNA polymerase | Trans Gen Biotech |
Trans Start FastPfu Fly DNA polymerase | Trans Gen Biotech |
T4 DNA Ligase | Thermo Scientific |
Fast digest DpnⅠ | Thermo Scientific |
Fast digest KpnⅠ | Thermo Scientific |
Fast digest NotⅠ | Thermo Scientific |
Fast digest EcoRⅠ | Thermo Scientific |
Fast digest PstⅠ | Thermo Scientific |
Fast digest XbaⅠ | Thermo Scientific |
Fast digest SpeⅠ | Thermo Scientific |
5. Kit
Kit | From |
---|
GeneJET Gel Exteaction Kit | Thermo Scientific |
HiPure Gel Pure DNA Micro Kit | Magen |
TIAN prep Mini plasmid Kit | TIANGEN BIOTECH (BEIJING) CO.,LTD. |
TIANamp Yeast DNA Kit | TIANGEN BIOTECH (BEIJING) CO.,LTD. |
Reactive oxygen species (ROS) test kit (Cat. No.: E004 Chemiluminescence) | Nanjing Jiancheng Bioengineering Institute |
6. Agarose Gel Electrophoresis
Reagent | From / Preparation method |
---|
Golden View (BIOWEST REGULAR AGAROSE G-10 ) | Biomed |
1×TAE | Diluted with 50×TAE |
50×TAE | Tris 242g, Na2EDTA·2H2O 37.2g, 57.1ml glacial acetic acid, 1L water |
7. Culture medium
Culture medium | Preparation method |
---|
LB(for E.coli) | 10g Tryptone peptone, 5g Yeast extract, 5gNaCl, dissolve in 1.0L water (add extra 20g agar powder for solid medium ) |
YPD(for yeast) | 20g Tryptone peptone, 10g Yeast extract, dissolve in 950ml water. Autoclave. Add 50ml 40%Glucose (add extra 20g agar powder for solid medium ) |
SD-His(for yeast) | 6.7g Yeast Nitrogen Base Without Amino acids, 0.6g Drop-out amino acid mixture, 100mg Leucine, 20mg Tryptophane, 20mg uracil, dissolve in 1.0L water. Autoclave. Add 50ml 40%Glucose (add extra 20g agar powder for solid medium ) |
SD-Leu(for yeast) | 6.7g Yeast Nitrogen Base Without Amino acids, 0.6g Drop-out amino acid mixture, 20mg Histidine, 20mg Tryptophane, 20mg uracil, dissolve in 1.0L water. Autoclave. Add 50ml 40%Glucose (add extra 20g agar powder for solid medium ) |
SD-Ura(for yeast) | 6.7g Yeast Nitrogen Base Without Amino acids, 0.6g Drop-out amino acid mixture, 20mg Histidine, 100mg Leucine, 20mg Tryptophane, dissolve in 1.0L water. Autoclave. Add 50ml 40%Glucose (add extra 20g agar powder for solid medium ) |
SD-Trp(for yeast) | 6.7g Yeast Nitrogen Base Without Amino acids, 0.6g Drop-out amino acid mixture, 20mg Histidine, 100mg Leucine, 20mg uracil, dissolve in 1.0L water. Autoclave. Add 50ml 40%Glucose (add extra 20g agar powder for solid medium ) |
8. Antioxidant
Antioxidant | From |
---|
L-Ascorbic acid | Tianjin Fuchen Chemical Reagent Factory |
Quercetin | Beijing Dongyan Biotech Co.,Ltd |
Catechol | Sinopharm Chemical Reagent Co.,Ltd |
Resveratrol | J&K Scientific Ltd |
β-Carotene | J&K Scientific Ltd |
trans-Ferulic acid | J&K Scientific Ltd |
Gallic acid | J&K Scientific Ltd |
Caffeic acid | J&K Scientific Ltd |
Vitamin E | solarbio |
9. Host cells
Name | From |
---|
TOP10 | Biomed |
DH5α | Biomed |
CENPK2-1C | iBT SynBios |
10. Other reagents
Name | Preparation method / From |
---|
50% Glycerol | Use distilled water to dilute 50ml glycerol twice as much |
dd H2O | / |
Absolute Ethyl Alcohol | / |
40% Glucose | 20g glycerol dissolved in 90 ml of water. |
PEG (50%w/v) | / |
1.0M LiAc | 6.6g LiAC dissolved in 100 ml of water. |
0.1M LiAc | 0.66g LiAC dissolved in 100 ml of water. |
SS-DNA (10.0mg/ml) | Solarbio |
30% H2O2 | Beijing Chemical Works |