Difference between revisions of "Team:BIT-China"

 
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                                    <a href="#intro">Introduction
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                                    <a href="#measure">Measure
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                                    <a href="#approach">Approach
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                                    </a>
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                                    <a href="#target">Target
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                                    </a>
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                                <li>
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                                    <a href="#contact">Contact us
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                                    </a>
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            <a>PROJECT</a>
                        <a id="nav-toggle" class="nav_slide_button" href="#">
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            <ul>
                            <span></span>
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                <li><a href="https://2018.igem.org/Team:BIT-China/Background">Background</a></li>
                        </a>
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                 <li><a href="https://2018.igem.org/Team:BIT-China/Description">Description</a></li>
                    </div>
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                 <li><a href="https://2018.igem.org/Team:BIT-China/Design">Idea & Design</a></li>
                </div>
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             </ul>
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         </li>
                    <div class="col-md-8 col-md-offset-2 text-center inner">
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                        <h1 class="animated fadeInDown">BIT-China</h1>
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                        <img src="https://static.igem.org/mediawiki/2018/d/da/T--BIT-China--iGEM2018_logo.png" height="100" width="100" />
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                    </div>
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                </div>
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             </div>
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         </section>
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    </header>
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        <li>
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            <a>EXPERIMENTS</a>
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            <ul>
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                <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsRegulator">Regulator</a></li>
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                <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsFeedback">Feedback</a></li>
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                <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsOutput">Output</a></li>
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                <li><a href="https://2018.igem.org/Team:BIT-China/Results">Results</a></li>
 +
            </ul>
 +
        </li>
  
    <section class="dark-bg text-center section-padding contact-wrap" id="intro">
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        <li>
         <div class="container">
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            <a>MODELING</a>
             <div class="row">
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            <ul>
                 <div class="col-md-8 col-md-offset-2 wp1">
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                <li><a href="https://2018.igem.org/Team:BIT-China/Model">Overview</a></li>
                    <h1 class="arrow text-center">About us</h1>
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                <li><a href="https://2018.igem.org/Team:BIT-China/FluorescentProbesModel">Fluorescent Probe Model </a></li>
                    <p>This year, we will focus on how to identify the antioxidant capacity of antioxidants.. Antioxidants has
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                <li><a href="https://2018.igem.org/Team:BIT-China/H2O2DecompositionModel">H<sub>2</sub>O<sub>2</sub>
                        been widely used in foods, Health products and cosmetic. But is there any measure way to know which
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                        Decomposition Model</a></li>
                        antioxidant is better?</p>
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                 </div>
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                <li><a href="https://2018.igem.org/Team:BIT-China/roGFP2-Orp1MichaelisEquationModel">roGFP2-Orp1
             </div>
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                        Michaelis equation Model</a></li>
 +
            </ul>
 +
         </li>
 +
 
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        <li>
 +
            <a>HUMAN PRACTICES</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/HPOverview">Overview</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Human_Practices">Integrated Human Practices</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Public_Engagement">Education & Public Engagement</a></li>
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                <li><a href="https://2018.igem.org/Team:BIT-China/Collaborations">Collaborations</a></li>
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             </ul>
 +
        </li>
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 +
        <li>
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            <a>NOTEBOOK</a>
 +
            <ul>
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                <li><a href="https://2018.igem.org/Team:BIT-China/Notebook">Lab Book</a></li>
 +
                 <li><a href="https://2018.igem.org/Team:BIT-China/Protocols">Methodology / Protocols</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Equipment">Material & Equipment</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/InterLab">Measurement / InterLab</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Safety">Safety</a></li>
 +
            </ul>
 +
        </li>
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 +
        <li>
 +
            <a>ACHIEVEMENTS</a>
 +
            <ul>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/JudgingForm">Judging Form</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Parts">Parts</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Improve">Improve</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Applied_Design">Applied Design</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Demonstrate">Demonstrate</a></li>
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            </ul>
 +
        </li>
 +
 
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        <li>
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            <a>TEAM</a>
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            <ul>
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                 <li><a href="https://2018.igem.org/Team:BIT-China/Team">Members</a></li>
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                <li><a href="https://2018.igem.org/Team:BIT-China/Attributions">Attributions</a></li>
 +
                <li><a href="https://2018.igem.org/Team:BIT-China/Gallery">Gallery</a></li>
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             </ul>
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        </li>
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    </ul>
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    <a href="https://2018.igem.org/Team:BIT-China"><img id="imgA" class="imgA-new-pos" src="https://static.igem.org/mediawiki/2018/4/46/T--BIT-China--iGEM2018-A_img.png" /></a>
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                     <h1 class="arrow text-center">Measure ways before us</h1>
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                    <p>1. Most of the current detection methods for antioxidant properties are based on chemical assays, which
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                        has limited value for us since the redox reaction in vivo is quite different from that in vitro.
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             <div id="text1" style="z-index:1;position: absolute;left:calc(50vw - 250px);bottom:calc(50vh - 230px);">
                        <br> 2. Others also consider to transfer redox reactions to the intracellular environment by adding H2O2
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                 <p class="p_style" style="width:350px;font-size:16px !important;">
                        and different antioxidants in order, through observing whether the yeast died or not, they could
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                     <font size="5" face="kg_second_chances_solidRg">“A”</font> indicates antioxidant, and can also
                        know which antioxidant would work better. However, people still looking forward a more gentle way
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                    represent the best in the grade rating. So the core of our project is to build an antioxidant
                        of representation.
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                    detecting system to rank antioxidants.
                        <br>
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                     <h1 class="arrow text-center">What we are trying to do</h1>
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                    <p>We are going to modified yeast to identify the antioxidant capacity of antioxidants. Single-cell eukaryotic
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                        yeasts have a similar apoptotic process as animal cells, and due to S. cerevisiae have advantages,
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             <div id="text2" style="position: absolute;left:calc(50vw - 365px);top:calc(50vh + 135px);">
                        Saccharomyces cerevisiae is an important model organism for studying apoptosis.</p>
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                 <p class="p_style" style="width:520px;">
                </div>
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                     <font size="5" face="kg_second_chances_solidRg">Antioxidants</font> are <br>compounds that inhibit
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                    oxidation.They can scavenge the excess ROS in the organisms. The antioxidants mentioned here is the
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                    one working in our bodies, helping us defect the damage of oxidation.
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                </p>
 
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                    <h1 class="arrow text-center">how to achieve that</h1>
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                    <p>To achieve that goal, our project will be separate into 4 parts:
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                <p class="p_style" style="width:360px;">
                        <br> 1. Regulate
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                    <font size="5" face="kg_second_chances_solidRg">Oxidation</font> damage is very common in our daily
                        <br> Firstly, we will over express gene ndi1 (which may express ubiquinone oxidoreductase, transfers
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                    life. It may be caused by UV in the sun, unhealthy living habits, irregular work and rest times,
                        electrons from NADH to ubiquinone in the respiratory chain. It has been proved that over express
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                    and its accumulation will eventually infect the normal function of our body, lead to gene mutation,
                        ndi1 could accumulate ROS. [1]) to accumulate ROS (Reactive oxygen species). Besides, through knocking
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                    cell cancer and aging. Therefore, antioxidants are urgently needed to defended the damage of
                        out gene yca1 (also known as MCA1, may expresses Ca(2+)-dependent cysteine protease MCA1. MCA1 could
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                    oxidation.
                        regulate apoptosis upon H2O2 treatment. Once knock it out, yeast may survive from high oxidative
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                </p>
                        stress. [2]) we will improve the tolerance of ROS, which means our yeast will stay alive during the
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                        whole process of detection.
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                        <br> 2. Feedback
+
                        <br> To ensure our yeast won’t die from large accumulation of ROS, we need to promptly turn off the
+
                        expression of ROS. At the same time, this will also meet the detection range of ro-GFP.
+
                        <br> 3. Input
+
                        <br> after that, we will add different antioxidants into the medium, we will standardize the externally
+
                        added antioxidants to make the results more feasible.
+
                        <br> 4. Output
+
                        <br> We will express orp-ro-GFP in our yeast to sense the ROS remaining, (it’s a kind of Fusion protein,
+
                        which will become oxidized and turn into orp-ox-GFP once react with ROS, and when the amount of ROS
+
                        drop, it will back to orp-ro-GFP. Its two different states correspond to different wavelengths of
+
                        excitation light, which has been described and used in
+
                        <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2296000">Cornell University 2017</a> ) to know the residual amount of yeast endogenous ROS in different states,
+
                        so that we can compare the performance of different antioxidants, and conclude which antioxidant
+
                        is better.
+
                        <br>
+
                    </p>
+
                </div>
+
 
             </div>
 
             </div>
 +
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         </div>
 
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                    <font size="5" face="kg_second_chances_solidRg">Even</font> though antioxidant is so important,
 +
                    there’s no standard ways to tell us which antioxidant works better.
 +
                </p>
 
             </div>
 
             </div>
  
 +
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                        <h2>
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                     <font size="5" face="kg_second_chances_solidRg">Our</font> goal is to build a system with function
                            <i class="fa fa-envelope"></i>
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                    of detecting the antioxidants and has the advantages of high biological relevance, simple
                            <span>Email</span>
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                    operation, low-cost, good reproducibility, accuracy, and high sensitivity for antioxidant
                        </h2>
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                    detection. More importantly, we hope to establish an efficient standard for evaluating antioxidants
                        <p>
+
                     through our system
                            <a href="mailto:bit_china@163.com">bit_china@163.com</a>
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                 </p>
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                        <p>bit_china</p>
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Latest revision as of 01:27, 18 October 2018

“A” indicates antioxidant, and can also represent the best in the grade rating. So the core of our project is to build an antioxidant detecting system to rank antioxidants.

Antioxidants are
compounds that inhibit oxidation.They can scavenge the excess ROS in the organisms. The antioxidants mentioned here is the one working in our bodies, helping us defect the damage of oxidation.

Oxidation damage is very common in our daily life. It may be caused by UV in the sun, unhealthy living habits, irregular work and rest times, and its accumulation will eventually infect the normal function of our body, lead to gene mutation, cell cancer and aging. Therefore, antioxidants are urgently needed to defended the damage of oxidation.

Even though antioxidant is so important, there’s no standard ways to tell us which antioxidant works better.

Our goal is to build a system with function of detecting the antioxidants and has the advantages of high biological relevance, simple operation, low-cost, good reproducibility, accuracy, and high sensitivity for antioxidant detection. More importantly, we hope to establish an efficient standard for evaluating antioxidants through our system