Difference between revisions of "Team:IIT Delhi/Attributions"

 
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   <div class="caption">
     <span class="border" >Text anyone?</span>
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     <span class="border" >Project Advisors</span>
 
   </div>
 
   </div>
 
</div>
 
</div>
 +
  
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<div style="color: black;background-color:white;text-align:center;padding:50px 80px;text-align: justify;width: 75%;margin: auto;">
 
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  <p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814001 = pLTL
 
    <br>
 
      </p>
 
      <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;"> The pLTL (Lac-Tet-Lac) is a hybrid promoter. It is a promoter composed of the operator sequences of pTet and the pLac promoter. There are multiple benefits of using the pLTL promoter.
 
</p>
 
   
 
      <ul style="font-size:20px;" >
 
      <li style="font-family: 'Lato', sans-serif;font-weight:400;">The hybrid pLTL shows almost complete repression on being repressed, and on induction (by IPTG and/or aTc), the hybrid pLTL promoter shows a 1.4-2 times higher expression.</li>
 
      <li style="font-family: 'Lato', sans-serif;font-weight:400;">The hybrid pLTL promoter also permits flexible gene expression because it can be utilized under either or both repression controls (LacI and TetR) simultaneously.</li>
 
   
 
    </ul>
 
<br>
 
  
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814008 = rrnB T1 Terminator + T7Te Terminator
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<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">
    <br>
+
      </p>
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      <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;"> Our part is double terminator composed of rrnB T1 Terminator(BBa B0010) and T7 Te Terminator(BBa B0012). Both of these are forward terminators that are extensively used in E. coli.
+
</p>
+
<br>
+
  
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814009 = mKate2
+
<b> Dr. Zia Shaikh </b> <br>
 +
Dr. Zia Shaikh is an assistant professor at DBEB, IIT Delhi and is the Professor-in-charge at iGEM IIT Delhi. He has been associated with the team since the past 3 years and has played a vital role in ensuring smooth functioning of the team. He provided us with a well-furnished lab for our project and was instrumental in getting funds for our team. Also, he helped us in the various aspects of our project, despite it not being his core area of research.<br><br>
  
    <br>
 
      </p>
 
      <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;"> Mkate2 - mKate2 is a red fluorescent protein derived from Entacmaea quadricolor.  It possesses fluorescence with excitation maxima at 588 nm and emission maxima at 588 and 633 nm, mKate2 is almost 3-fold brighter than mKate. mKate2 can be used in  labelling applications along with blue, cyan, green, yellow, and red fluorescent dyes. Its high pH-stability with pKa=5.4 makes it useful for imaging in acidic organelles, such as late and recycling endosomes and lysosomes.
 
  
</p>
+
<b>Dr. Stefan </b><br>
<br>
+
Dr. Stefan is a visiting faculty, from Germany, at DBEB, IIT Delhi. He has been a guiding light for iGEM IIT Delhi since 2015. Even though he is a specialist in Molecular Biology, he was instrumental in our project’s computational analysis as well as team organization and management. He spent countless nights helping us troubleshoot our project and enabled us to present our project in a more detailed manner.<br><br>
  
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814010 = rrnB T1 Terminator
+
<b>Mr. Kshitij Rai</b><br>
 +
Kshitij Rai, a graduate from IIT Delhi headed iGEM IIT Delhi for the past two years. Currently, he is pursuing PhD in Systems, Synthetic, and Physical Biology from Rice University, Texas.<br><br>
  
    <br>
+
<b>Mr. Abhilash Patel</b><br>
      </p>
+
Abhilash Patel is pursuing PhD in Electrical Engineering from IIT Delhi. He is currently the team leader of iGEM IIT Delhi.
    <br>
+
</p>
 
</div>
 
</div>
  
<div class="bgimg-2">
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<div class="bgimg-1">
 
   <div class="caption">
 
   <div class="caption">
     <span class="border" >Text anyone?</span>
+
     <span class="border" >Professors</span>
 
   </div>
 
   </div>
 
</div>
 
</div>
 +
  
<div style="position:relative;">
+
<div style="color: black;background-color:white;text-align:center;padding:50px 80px;text-align: justify;width: 75%;margin: auto;">
  <div style="color: #777;background-color:white;text-align:center;padding:50px 80px;text-align: justify;">
+
  <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">
 
+
<b>Prof. Atul Narang (DBEB)</b><br>
  <p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814007 = sfGFP_ssrA
+
Dr. Atul Narang is the Head of Department of Department of Biochemical Engineering and Biotechnology (DBEB), IIT Delhi. He has supported us remarkably in terms of the lab space, resources and facilities.<br><br>
  
 +
<b>Prof.Shaunak Sen (Department of Electrical Engineering)</b><br>
 +
Prof. Shaunak Sen has assisted us profoundly with Microscopes, Plate-readers, and protocols. We would also like to thank him for his valuable advices on the idea Square Wave Generator, especially during the brainstorming sessions at the beginning.<br><br>
  
    <br>
+
<b>Prof. James Gomes (Kusuma School of Biological Sciences)</b><br>
      </p>
+
We would like to greatly acknowledge Prof. James Gomes for his invaluable guidance towards the idea of the project. He has also assisted us with mathematical analysis, design and construction and possible topologies of the project.<br><br>
      <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">Our part consists of sfGFP appended with a ssrA(LVA) deg-tag. It has been codon optimised for E. coli.  This part is useful as it has high intensity as compared to other gfp variants as well as faster degradation rates and shorter reporter lifetimes.
+
  
 +
<b>Dr.Vivekanandan Perumal ( Kusuma School of Biological Sciences)</b><br>
 +
Dr. Vivekanandan is an assistant professor at KSBS, IIT Delhi. He helped us in developing our cloning strategy and provided us with various lab equipments at crucial times.<br><br>
  
 +
<b>Dr.Ashish Misra (DBEB)</b><br>
 +
Dr. Ashish Misra is an assistant professor at DBEB, IIT Delhi and is a Professor mentor at iGEM IIT Delhi. He joined the team in 2016 and has helped us in troubleshooting our project and gaining more insight into it.<br><br>
 
</p>
 
</p>
 +
</div>
  
<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">Superfolder GFP is a basic (constitutively fluorescent) green fluorescent protein derived from Aequorea victoria. It has an emission wavelength of 510 nm and excitation wavelength of 485nm. It has a robust folding characteristic. The superfolder mutations also make the folding of GFP tolerant of mutations that would otherwise reduce the folding yield of GFP.
+
<div class="bgimg-1">
 +
  <div class="caption">
 +
    <span class="border" >PhD. Researchers</span>
 +
  </div>
 +
</div>
 +
  
 +
<div style="color: black;background-color:white;text-align:center;padding:50px 80px;text-align: justify;width: 75%;margin: auto;">
 +
<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">
  
 +
<b>Ms.Anamika Chauhan</b><br>
 +
Ms. Anamika is a PhD student at DBEB, IIT Delhi and has been a part of team iGEM IIT Delhi since 2015. She was crucial to our Wet Lab experiments and troubleshooting and helped us improve our lab procedures. Moreover, she has been very supportive at crucial moments faced during the project.<br><br>
 +
 +
<b>Mr. Arun Thapa</b><br>
 +
Mr. Arun is a PhD student at DBEB, IIT Delhi and has been a mentor for iGEM IIT Delhi since 2016. He played a significant role in Wet lab experiments and troubleshooting and gave us crucial advice at critical times.<br><br>
 
</p>
 
</p>
 +
</div>
  
<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">Our ssrA degtag is the gfp(LVA) that has a single (A → G) point mutation in nucleotide 349, resulting in an Asp117 → Gly117 (D117G) amino acid change. This point mutation does not appear to change the fluorescence spectrum of gfp(LVA). The LVA tag has been reported to lead to fast protein degradation, degrading GFP with rate -0.018 per minute. This corresponds to in vivo half-lives of mature Gfp(LVA) of approximately 40 min.
+
<div class="bgimg-1">
 +
  <div class="caption">
 +
    <span class="border" >Assistance form Various Labs</span>
 +
  </div>
 +
</div>
 +
  
 +
<div style="color: black;background-color:white;text-align:center;padding:50px 80px;text-align: justify;width: 75%;margin: auto;">
 +
<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">
  
 +
<b>UG Lab</b><br>
 +
Mr.Gulshan is the Lab Assistant of the Undergrad Lab, he has played a crucial role in terms of providing lab facilities and access throughout the entire tenure of the project.<br><br>
  
 +
<b>DyCoBS</b><br>
 +
Mr. Venkat and Mr. Soumyadeep have assisted us in the Dynamic Control of Biological Systems Lab, and helped us refine our lab protocols and methods.<br><br>
 
</p>
 
</p>
 +
</div>
  
<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">References</p>
+
<div class="bgimg-1">
<ol style="font-size: 20px;">  
+
  <div class="caption">
 
+
    <span class="border" >Human Practices</span>
<li style="font-family: 'Lato', sans-serif;font-weight:400;">Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology. 2006 Jan;24(1):79.</li>  
+
   </div>
 
+
</div>
<li style="font-family: 'Lato', sans-serif;font-weight:400;">Andersen, J. B. et al. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64, 2240–6 (1998).
+
</li>
+
 
+
 
+
   </ol>
+
 
+
<br>
+
 
+
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814011 = attP TP901-1
+
 
+
 
+
    <br>
+
      </p>
+
      <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">AttP TP901-1 - It is the AttP site of TP901-1 integrase (serine based recombinase enzyme). TP901-1 integrase is an enzyme that has been isolated from TP901-1 phage. It is responsible for catalysing site-specific recombination at AttP and AttB sites. The recombination mechanism depends on the orientation of the AttP and AttB sites. These enzymes help the virus integrate its DNA into the bacterial genome. TP901-1 is widely used in the construction of logic gates and modification of DNA sequences.
+
 
+
  
 +
<div style="color: black;background-color:white;text-align:center;padding:50px 80px;text-align: justify;width: 75%;margin: auto;">
 +
<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">
 +
<b>Schools Across Delhi</b><br>
 +
We conducted synthetic Biology workshops various School across the city: Modern School, Bal Bharti School, Sanskriti School, SpringDales, Bloom Public School,Carmel Convent School. We express our gratitude towards all of these schools for their support in this endeavour.<br><br>
 +
 +
<b>Professors at IIT Delhi</b><br>
 +
We would also like to thank Prof. P. V. Madhusudhan Rao (Mechanical Engineering Department) for their support to conduct human practices at Institute level.<br>.<br>
 
</p>
 
</p>
 
+
</div>
<br>
+
  
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814012 = BxbI Integrase + ssrA(LVA) deg tag
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<div class="bgimg-1">
 +
  <div class="caption">
 +
    <span class="border" >Sponsors</span>
 +
  </div>
 +
</div>
 +
  
 +
<div style="color: black;background-color:white;text-align:center;padding:50px 80px;text-align: justify;width: 75%;margin: auto;">
 +
<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;"><img src="https://static.igem.org/mediawiki/2018/3/35/T--IIT_Delhi--sponsors.png" alt="Smiley face" style="float:right;width:600px;margin:15px">
  
    <br>
+
<b>Mr. Brij Mohan Gupta:</b><br>
      </p>
+
We thank Mr. Brij Mohan Gupta aka Bade Bhaiyya for his hearty financial Contributions and moral support for our whole team.<br><br>
      <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">BxbI Integrase triggers attP × attB recombination. The product of attP × attB recombination is an integrated prophage flanked by two new recombination sites, attL and attR, each containing half sites derived from attP and attB. In the absence of accessory factors the integrases mediate unidirectional recombination between attP and attB with greater than 80% efficiency. In the presence of a phage-encoded accessory protein, the recombination directionality factor (RDF) the attP × attB recombination is inhibited and the attL × attR recombination is stimulated.
+
Bxb1 integrase yields approximately two-fold more recombinants  and displays about two fold less damage to the recombination sites than other phage-encoded serine integrases.
+
  
Our BxbI Integrase has a ssrA deg tag attached to it for faster degradation rates.
+
<b>Snapgene:</b><br>
 +
We thank our Snapgene for providing us licenses to its software which made the work of designing and analyzing the gene sequences very remarkably convenient.<br><br>
  
 +
<b>MathWorks:</b><br>
 +
We express our gratitude to Mathworks for providing us with Licenses to MATLAB and Simulink which provided us with a great tool for computational modeling.<br><br>
  
 +
<b>Ketto Donors:</b><br>
 +
We would also like to express our gratitude towards all the people who funded our project through our Ketto Campaign Page.<br><br>
  
 +
<b>Previous Sponsors:</b><br>
 +
We thank our sponsors for their support in the previous years of iGem IIT Delhi: Mahindra, Woodland, Coding Blocks, Medanta, DHL, Prompt<br><br>
 
</p>
 
</p>
 
<br>
 
 
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814013 = attB TP901-1
 
 
 
    <br>
 
      </p>
 
      <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">AttB TP901-1 -  It is the AttB site of TP901-1 integrase (serine based recombinase enzyme). TP901-1 integrase is an enzyme that has been isolated from TP901-1 phage. It is responsible for catalysing site-specific recombination at AttP and AttB sites. The recombination mechanism depends on the orientation of the AttP and AttB sites. These enzymes help the virus integrate its DNA into the bacterial genome. TP901-1 is widely used in the construction of logic gates and changing DNA sequence.
 
 
 
 
</p>
 
 
<br>
 
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814014 = complement of B0034, RBS on the antisense strand, twin exists
 
    <br>
 
      </p>
 
<br>
 
 
 
  </div>
 
 
</div>
 
</div>
  
<div class="bgimg-3">
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<div class="bgimg-1">
 
   <div class="caption">
 
   <div class="caption">
     <span class="border">Some random text </span>
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     <span class="border" >Team Members</span>
 
   </div>
 
   </div>
 
</div>
 
</div>
 +
  
<div style="position:relative;">
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<div style="color: black;background-color:white;text-align:center;padding:50px 80px;text-align: justify;width: 75%;margin: auto;">
  <div style="color: #777;background-color:white;text-align:center;padding:50px 80px;text-align: justify;">
+
<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">
   
+
<b>Wetlab:</b><br>
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814015 = pLac(lambda) hybrid
+
Saksham Sharma, Abhilash Patel, Vasu Jain, Priyanka Singh, Shubham Jain, Neha Arora, Soumya Gupta<br><br>
  
 +
<b>Computational Modeling:</b><br>
 +
Abhilash Patel, Shubham Jain, Vedant Raval,Vasu Jain<br><br>
  
    <br>
+
<b>Human Practices:</b><br>
      </p>
+
Saksham Sharma, Vasu Jain, Shubham Jain, Priyanka Singh, Neha Arora, Soumya Gupta, Ankush Barman, Aman Sahu, Vedant Raval<br><br>
      <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">pLac - Lambda hybrid - is a hybrid promoter consisting of Lac and Lambda operator sites in the core region. This hybrid promoter can be induced in the presence of  IPTG or
+
</p>
+
<br>
+
  
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814017 = attP Bxb1
+
<b>Wiki Development:</b><br>
 
+
Aman Kumar Sahu, Vedant Raval, Divya Choudhary, Saksham Sharma, Priyanka Singh, Vasu Jain, Shubham Jain<br><br>
 
+
 
+
    <br>
+
      </p>
+
      <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">AttP Bxb1 - It is the AttP site of Bxb1 integrase (Serine based recombinase). Bxb1 integrase carries out site-specific recombination by catalysing unidirectional recombination to produce AttL and AttR sites from AttP and AttB. This switching capacity allows them to be used in the design of toggle switches.
+
  
 +
<b>Creative, Design and Content Development:</b><br>
 +
Saksham Sharma, Priyanka Singh, Vasu Jain, Shubham Jain, Neha Arora, Soumya Gupta<br><br>
  
 +
<b>Marketing:</b><br>
 +
Saksham Sharma, Vedant Raval, Aman Sahu, Soumya Gupta, Vasu Jain<br>
 
</p>
 
</p>
<br>
+
</div>
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814018 = attB Bxb1
+
 
+
 
+
    <br>
+
      </p>
+
      <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">AttB Bxb1 - It is the AttB site of Bxb1 integrase (Serine based recombinase). Bxb1 integrase carries out site-specific recombination by catalysing unidirectional recombination to produce AttL and AttR sites from AttP and AttB. This switching capacity allows them to be used in the design of logic gates.
+
  
 +
<div class="bgimg-1">
 +
  <div class="caption">
 +
    <span class="border" >Research Institute</span>
 +
  </div>
 +
</div>
 +
  
 +
<div style="color: black;background-color:white;text-align:center;padding:50px 80px;text-align: justify;width: 75%;margin: auto;">
 +
<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">
 +
We thank our institute, IIT Delhi wholeheartedly.<br>
 
</p>
 
</p>
<br>  
+
</div>
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814019 = P7 Promoter
+
  
 +
<div class="bgimg-1">
 +
  <div class="caption">
 +
    <span class="border" >iGEM Foundation</span>
 +
  </div>
 +
</div>
 +
  
 +
<div style="color: black;background-color:white;text-align:center;padding:50px 80px;text-align: justify;width: 75%;margin: auto;">
 +
  
 
+
<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">
    <br>
+
Last but not the least, we are extremely grateful to International Genetic Engineering Machine Foundation for creating such an incredible platform 'iGEM Competition' where multidisciplinary students get the opportunity to work and build genetically engineered systems using standard biological parts(BioBricks) to solve real-world challenges. Moreover, it brings all such students and their projects together annually.<br>
      </p>
+
      <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">Constitutive P7 promoter - It is the complement of the constitutive P7 promoter so as to initiate transcription in the reverse direction.
+
  
 
</p>
 
</p>
 +
</div>
  
<br>
 
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814021 = BxbI-Xis  + ssrA(LVA) deg tag------------
 
  
  
    <br>
 
      </p>
 
      <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">Xis-BxbI_ssrA deg tag - Consists of bxb1 excisionase followed by ssrA degradation tag. Bxb1-Xis  catalyses the conversion of AttL and AttR sites to AttP and AttB sites when expressed with Bxb1 integrase, thereby reverting the recombination caused by integrase. ssrA deg tag degrades the Bxb1-Xis formed as an increase in the amount of excisionase renders the system inefficient. This property allows Bxb1 to be used in the construction of logic gates.
 
</p>
 
  
<br>
 
  
<p style="font-size: 30px; font-family: 'Roboto', sans-serif;font-weight:700;">BBa_K2814022 = attB BxbI (reverse orientation)
 
  
  
     <br>
+
     <div style="background-color: white">
      </p>
+
      <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;">AttB-BxbI (Reverse Orientation) - Contains AttB site of Bxb1 integrase in the reverse orientation (Reverse of  BBa_K2814018). Bxb1 integrase carries out site-specific recombination by catalysing unidirectional recombination to produce AttL and AttR sites from AttP and AttB. This switching capacity allows them to be used in the design of logic gates.
+
 
+
</p>
+
 
+
<br>
+
 
+
 
+
  </div>
+
</div>
+
 
+
    <!--/**/-->
+
 
+
       
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<div style="background-color: white">
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         <div  style="background-color: #555;color:black;font-size: 35px;text-align: center;font-family: sans-serif;"><h4 style="color: white">Contact us</h4></div>
 
         <div  style="background-color: #555;color:black;font-size: 35px;text-align: center;font-family: sans-serif;"><h4 style="color: white">Contact us</h4></div>
 
             <div style="text-align: center;margin-bottom: -136px;"><h4 style="color:black;font-size: 50px;"><u class="fx" style="cursor: pointer;text-align: center">Address</u></h4><p style="text-align: center;margin-bottom: 16px">Undergraduate Laboratory<br>
 
             <div style="text-align: center;margin-bottom: -136px;"><h4 style="color:black;font-size: 50px;"><u class="fx" style="cursor: pointer;text-align: center">Address</u></h4><p style="text-align: center;margin-bottom: 16px">Undergraduate Laboratory<br>
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Latest revision as of 02:52, 18 October 2018

iGEM IIT Delhi

Project Advisors

Dr. Zia Shaikh
Dr. Zia Shaikh is an assistant professor at DBEB, IIT Delhi and is the Professor-in-charge at iGEM IIT Delhi. He has been associated with the team since the past 3 years and has played a vital role in ensuring smooth functioning of the team. He provided us with a well-furnished lab for our project and was instrumental in getting funds for our team. Also, he helped us in the various aspects of our project, despite it not being his core area of research.

Dr. Stefan
Dr. Stefan is a visiting faculty, from Germany, at DBEB, IIT Delhi. He has been a guiding light for iGEM IIT Delhi since 2015. Even though he is a specialist in Molecular Biology, he was instrumental in our project’s computational analysis as well as team organization and management. He spent countless nights helping us troubleshoot our project and enabled us to present our project in a more detailed manner.

Mr. Kshitij Rai
Kshitij Rai, a graduate from IIT Delhi headed iGEM IIT Delhi for the past two years. Currently, he is pursuing PhD in Systems, Synthetic, and Physical Biology from Rice University, Texas.

Mr. Abhilash Patel
Abhilash Patel is pursuing PhD in Electrical Engineering from IIT Delhi. He is currently the team leader of iGEM IIT Delhi.

Professors

Prof. Atul Narang (DBEB)
Dr. Atul Narang is the Head of Department of Department of Biochemical Engineering and Biotechnology (DBEB), IIT Delhi. He has supported us remarkably in terms of the lab space, resources and facilities.

Prof.Shaunak Sen (Department of Electrical Engineering)
Prof. Shaunak Sen has assisted us profoundly with Microscopes, Plate-readers, and protocols. We would also like to thank him for his valuable advices on the idea Square Wave Generator, especially during the brainstorming sessions at the beginning.

Prof. James Gomes (Kusuma School of Biological Sciences)
We would like to greatly acknowledge Prof. James Gomes for his invaluable guidance towards the idea of the project. He has also assisted us with mathematical analysis, design and construction and possible topologies of the project.

Dr.Vivekanandan Perumal ( Kusuma School of Biological Sciences)
Dr. Vivekanandan is an assistant professor at KSBS, IIT Delhi. He helped us in developing our cloning strategy and provided us with various lab equipments at crucial times.

Dr.Ashish Misra (DBEB)
Dr. Ashish Misra is an assistant professor at DBEB, IIT Delhi and is a Professor mentor at iGEM IIT Delhi. He joined the team in 2016 and has helped us in troubleshooting our project and gaining more insight into it.

PhD. Researchers

Ms.Anamika Chauhan
Ms. Anamika is a PhD student at DBEB, IIT Delhi and has been a part of team iGEM IIT Delhi since 2015. She was crucial to our Wet Lab experiments and troubleshooting and helped us improve our lab procedures. Moreover, she has been very supportive at crucial moments faced during the project.

Mr. Arun Thapa
Mr. Arun is a PhD student at DBEB, IIT Delhi and has been a mentor for iGEM IIT Delhi since 2016. He played a significant role in Wet lab experiments and troubleshooting and gave us crucial advice at critical times.

Assistance form Various Labs

UG Lab
Mr.Gulshan is the Lab Assistant of the Undergrad Lab, he has played a crucial role in terms of providing lab facilities and access throughout the entire tenure of the project.

DyCoBS
Mr. Venkat and Mr. Soumyadeep have assisted us in the Dynamic Control of Biological Systems Lab, and helped us refine our lab protocols and methods.

Human Practices

Schools Across Delhi
We conducted synthetic Biology workshops various School across the city: Modern School, Bal Bharti School, Sanskriti School, SpringDales, Bloom Public School,Carmel Convent School. We express our gratitude towards all of these schools for their support in this endeavour.

Professors at IIT Delhi
We would also like to thank Prof. P. V. Madhusudhan Rao (Mechanical Engineering Department) for their support to conduct human practices at Institute level.
.

Sponsors

Smiley face Mr. Brij Mohan Gupta:
We thank Mr. Brij Mohan Gupta aka Bade Bhaiyya for his hearty financial Contributions and moral support for our whole team.

Snapgene:
We thank our Snapgene for providing us licenses to its software which made the work of designing and analyzing the gene sequences very remarkably convenient.

MathWorks:
We express our gratitude to Mathworks for providing us with Licenses to MATLAB and Simulink which provided us with a great tool for computational modeling.

Ketto Donors:
We would also like to express our gratitude towards all the people who funded our project through our Ketto Campaign Page.

Previous Sponsors:
We thank our sponsors for their support in the previous years of iGem IIT Delhi: Mahindra, Woodland, Coding Blocks, Medanta, DHL, Prompt

Team Members

Wetlab:
Saksham Sharma, Abhilash Patel, Vasu Jain, Priyanka Singh, Shubham Jain, Neha Arora, Soumya Gupta

Computational Modeling:
Abhilash Patel, Shubham Jain, Vedant Raval,Vasu Jain

Human Practices:
Saksham Sharma, Vasu Jain, Shubham Jain, Priyanka Singh, Neha Arora, Soumya Gupta, Ankush Barman, Aman Sahu, Vedant Raval

Wiki Development:
Aman Kumar Sahu, Vedant Raval, Divya Choudhary, Saksham Sharma, Priyanka Singh, Vasu Jain, Shubham Jain

Creative, Design and Content Development:
Saksham Sharma, Priyanka Singh, Vasu Jain, Shubham Jain, Neha Arora, Soumya Gupta

Marketing:
Saksham Sharma, Vedant Raval, Aman Sahu, Soumya Gupta, Vasu Jain

Research Institute

We thank our institute, IIT Delhi wholeheartedly.

iGEM Foundation

Last but not the least, we are extremely grateful to International Genetic Engineering Machine Foundation for creating such an incredible platform 'iGEM Competition' where multidisciplinary students get the opportunity to work and build genetically engineered systems using standard biological parts(BioBricks) to solve real-world challenges. Moreover, it brings all such students and their projects together annually.

Contact us

Address

Undergraduate Laboratory
Department of Biotechnology and Biochemical Engineering, IIT Delhi