Difference between revisions of "Team:HUST-China/Composite part"
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<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Modeling overview">Modeling overview</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Modeling overview">Modeling overview</a></li> | ||
| − | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/ | + | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/model_of_systems">Model of systems</a></li> |
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Sort of three genes">Sort of three genes</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Sort of three genes">Sort of three genes</a></li> | ||
| − | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Software"> | + | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Software">Software</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
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<a href="#" data-toggle="dropdown" class="dropdown-toggle waves-effect waves-dark">HP<b class="caret"></b></a> | <a href="#" data-toggle="dropdown" class="dropdown-toggle waves-effect waves-dark">HP<b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| − | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/ | + | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Human Practices">Human Practices</a></li> |
| − | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/ | + | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Education_Engagement">Education&Engagement</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
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<a href="#" data-toggle="dropdown" class="dropdown-toggle waves-effect waves-dark">TEAM<b class="caret"></b></a> | <a href="#" data-toggle="dropdown" class="dropdown-toggle waves-effect waves-dark">TEAM<b class="caret"></b></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| − | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Team | + | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Team">Team Members</a></li> |
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Collaborations">Collaborations</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Collaborations">Collaborations</a></li> | ||
<li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Attributions">Attributions</a></li> | <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Attributions">Attributions</a></li> | ||
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</div> | </div> | ||
| − | <div class="col-md-12"> | + | <div class="col-md-12"><br/> |
| − | <p>BBa_K2533000, BBa_K2533001, BBa_K2533002, BBa_K2533003, BBa_K2533014</p> | + | <p>BBa_K2533000, BBa_K2533001, BBa_K2533002, BBa_K2533003, BBa_K2533014</p><br/> |
</div> | </div> | ||
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<div class="row"></div> | <div class="row"></div> | ||
</div> | </div> | ||
| − | <div class="col-md-12"> | + | <div class="col-md-12"><br/> |
| − | <p>BBa_K2533016, BBa_K2533017, BBa_K2533018, BBa_K2533019</p> | + | <p>BBa_K2533016, BBa_K2533017, BBa_K2533018, BBa_K2533019</p><br/> |
</div> | </div> | ||
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</table> | </table> | ||
</div> | </div> | ||
| − | <div class="col-md-12"> | + | <div class="col-md-12"><br/> |
| − | <p>BBa_K2533050, BBa_K2533048, BBa_K2533054, BBa_K2533049</p> | + | <p>BBa_K2533050, BBa_K2533048, BBa_K2533054, BBa_K2533049</p><br/> |
</div> | </div> | ||
<div class="col-md-6 col-md-offset-2" > <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/be/T--HUST-China--2018-composite_part-picture3.png.png"></div> | <div class="col-md-6 col-md-offset-2" > <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/be/T--HUST-China--2018-composite_part-picture3.png.png"></div> | ||
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| − | <div class="col-md-12"> | + | <div class="col-md-12"><br/> |
<p>Our photosynthetic microorganism system is consist of Synechocystis sp. PCC 6803(cyanobacteria) and Rhodopseudomonas palustris. They are all modified to produce lactate to improve the electrogenic efficiency of Shewanella(S.oneidensis).</p> | <p>Our photosynthetic microorganism system is consist of Synechocystis sp. PCC 6803(cyanobacteria) and Rhodopseudomonas palustris. They are all modified to produce lactate to improve the electrogenic efficiency of Shewanella(S.oneidensis).</p> | ||
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<td>PlacIq-lacI-Ptac-RBS-gapA</td> | <td>PlacIq-lacI-Ptac-RBS-gapA</td> | ||
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| − | + | ||
| − | + | ||
</tr> | </tr> | ||
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<div class="col-md-12"> | <div class="col-md-12"> | ||
| − | + | <br/> <p>BBa_K2533030, BBa_K2533031, BBa_K2533032, BBa_K2533033, BBa_K2533034, BBa_K2533036, BBa_K2533037 </p><br/> | |
</div> | </div> | ||
| − | <div class="col-md-4 col-md-offset-2" style="text-align: center;"> | + | <div class="col-md-4 col-md-offset-2" style="text-align: center;"><br/> |
<img class="img-responsive" src="https://static.igem.org/mediawiki/2018/f/f9/T--HUST-China--2018-composite_part-picture8.png.png"> | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/f/f9/T--HUST-China--2018-composite_part-picture8.png.png"> | ||
</div> | </div> | ||
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<div class="col-md-12"> | <div class="col-md-12"> | ||
| − | <p>BBa_K2533035, BBa_K2533038, BBa_K2533039 </p> | + | <br/><p>BBa_K2533035, BBa_K2533038, BBa_K2533039 </p><br/> |
</div> | </div> | ||
| − | <div class="col-md-5 col-md-offset-2" style="text-align: center;"> | + | <div class="col-md-5 col-md-offset-2" style="text-align: center;"><br/> |
<img class="img-responsive" src="https://static.igem.org/mediawiki/2018/3/3a/T--HUST-China--2018-composite_part-picture5.png.png"> | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/3/3a/T--HUST-China--2018-composite_part-picture5.png.png"> | ||
</div> | </div> | ||
| − | <div class="col-md-12"> | + | <div class="col-md-12"><br/> |
<p>Our MFC produces electricity by Shewanella(S.oneidensis) through transfer chemical energy into electric energy. As a result, we design these parts to strengthen the ability of Shewanella(S.oneidensis) to absorb lactate，with an intention to boost the efficiency of electrogenic microorganism system.</p> | <p>Our MFC produces electricity by Shewanella(S.oneidensis) through transfer chemical energy into electric energy. As a result, we design these parts to strengthen the ability of Shewanella(S.oneidensis) to absorb lactate，with an intention to boost the efficiency of electrogenic microorganism system.</p> | ||
Latest revision as of 03:03, 18 October 2018
Composite part
Part1: Photosynthetic microorganism system: optical energy→chemical energy
1.1 Synechocystis sp. PCC 6803(cyanobacteria)
| Name | Description |
|---|---|
| BBa_K2533000 | RBS-ldhD-RBS-lldP |
| BBa_K2533001 | RBS-ldhDC-RBS-lldP |
| BBa_K2533002 | RBS-ldhDnARSdR-lldP |
| BBa_K2533003 | RBS-ldhDARSdR-lldP |
| BBa_K2533014 | RBS-TH-RBS-gldA-RBS-LLDP |
| BBa_K2533016 | RBS-ldhD-FLAG-RBS-lldP-his tag |
| BBa_K2533017 | RBS-ldhDC-FLAG-RBS-lldP-his tag |
| BBa_K2533018 | RBS-ldhDnARSdR-FLAG-RBS-lldP-his tag |
| BBa_K2533019 | RBS-ldhDARSdR-FLAG-RBS-lldP-his tag |
BBa_K2533000, BBa_K2533001, BBa_K2533002, BBa_K2533003, BBa_K2533014
BBa_K2533016, BBa_K2533017, BBa_K2533018, BBa_K2533019
1.2. Rhodopseudomonas palustris
| Name | Description |
|---|---|
| BBa_K2533045 | RBS-mleS |
| BBa_K2533046 | RBS-lldP |
| BBa_K2533047 | RBS-ldhA |
| BBa_K2533048 | RBS-mleS-RBS-lldP |
| BBa_K2533049 | RBS-lldP-RBS-ldhA |
| BBa_K2533050 | mleS-lldp-ldhA |
| BBa_K2533051 | mleS-lldP-TT |
| BBa_K2533052 | RBS-lldP-RBS-ldhA-TT |
| BBa_K2533053 | RBS-mleS-RBS-lldP-RBS-ldhA-TT |
| BBa_K2533054 | RBS-ldhA-RBS-lldP |
| BBa_K2533055 | RBS-ldhA-RBS-lldP-TT |
BBa_K2533050, BBa_K2533048, BBa_K2533054, BBa_K2533049
Our photosynthetic microorganism system is consist of Synechocystis sp. PCC 6803(cyanobacteria) and Rhodopseudomonas palustris. They are all modified to produce lactate to improve the electrogenic efficiency of Shewanella(S.oneidensis).
Part2: Electrogenic microorganism system: chemical energy→electric energy
2.1. Shewanella(S.oneidensis)
| Name | Description |
|---|---|
| BBa_K2533030 | RBS-dld |
| BBa_K2533031 | RBS-lldEFG |
| BBa_K2533032 | RBS-dld-TT-Ptac-RBS-lldEFG |
| BBa_K2533033 | RBS-gapA |
| BBa_K2533034 | RBS-mdh |
| BBa_K2533035 | RBS-gapA-RBS-mdh-TT |
| BBa_K2533036 | RBS-pflB |
| BBa_K2533037 | RBS-fdh |
| BBa_K2533038 | RBS-pflB-RBS-fdh |
| BBa_K2533039 | RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh |
| BBa_K2533056 | PlacIq-lacI-Ptac-RBS-gapA |
BBa_K2533030, BBa_K2533031, BBa_K2533032, BBa_K2533033, BBa_K2533034, BBa_K2533036, BBa_K2533037
BBa_K2533035, BBa_K2533038, BBa_K2533039
Our MFC produces electricity by Shewanella(S.oneidensis) through transfer chemical energy into electric energy. As a result, we design these parts to strengthen the ability of Shewanella(S.oneidensis) to absorb lactate，with an intention to boost the efficiency of electrogenic microorganism system.
