Difference between revisions of "Team:SCU-China"

 
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     <title>Team:SCU-China - 2018</title>
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             Project
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        </a>
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        <ul class="dropdown-menu">
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             <li><a href="https://2018.igem.org/Team:SCU-China/Repressilator">Repressilator</a>
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             </li>
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            <li><a href="https://2018.igem.org/Team:SCU-China/Malatonion">Melatonin</a>
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             <li><a href="https://2018.igem.org/Team:SCU-China/InterLab">InterLab</a></li>
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                Modification</a>
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        <a href="https://2017.igem.org/Team:SCU_China/HP/Silver" class="dropdown-toggle"
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          data-toggle="dropdown">Human Practices
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            </li>
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            <li><a href="https://2017.igem.org/Team:SCU_China/HP/Silver/Survey">Survey</a>
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<div style="width:100%">
            </li>
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     <div>
 
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         <img style="width:100%;" src="https://static.igem.org/mediawiki/2018/5/52/T--SCU-China--headback.jpg" />
            <li><a href="https://2017.igem.org/Team:SCU_China/HP/Silver/Meetup">Meet-up</a>
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            </li>
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            <li>
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                <a href="https://2017.igem.org/Team:SCU_China/HP/Silver/Engagement">Engagement</a>
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            </li>
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            <li><a href="https://2017.igem.org/Team:SCU_China/Collaborations">Collaboration</a>
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            </li>
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        </ul>
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    </li>
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    <li class="dropdown lee_nav_attri">
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        <a href="https://2017.igem.org/Team:SCU_China/Attributions">
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            Attribution
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        </a>
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    </li>
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    <li class="dropdown lee_nav_gold">
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        <a href="https://2017.igem.org/Team:SCU_China/HP/Gold_Integrated" class="dropdown-toggle"
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          data-toggle="dropdown">
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            Gold Medal
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        </a>
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        <ul class="dropdown-menu">
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            <li><a href="https://2017.igem.org/Team:SCU_China/HP/Gold_Integrated">Integrated
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                HP</a></li>
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            <li><a href="https://2017.igem.org/Team:SCU_China/HP/Improve">Improve</a></li>
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            <li><a href="https://2017.igem.org/Team:SCU_China/Model">Model</a></li>
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            <li><a href="https://2017.igem.org/Team:SCU_China/Demonstrate">Demonstrate</a></li>
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        </ul>
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    </li>
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    <li class="lee_nav_note">
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        <a href="https://2017.igem.org/Team:SCU_China/Notebook">
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            Notebook
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        </a>
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    </li>
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</div>
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<div class="column full_size" >
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    <h1>CRISProgrammer Description</h1>
+
    <p style = "align:left" size="2"> Inspired by the modularization, call-and-return and do-not-reinvent-the-wheel philosophy in computer programming design, we come up with the idea of using the dCas9 protein to manipulate the expression of proteins and implement complex logic in a single E. coli cell this year. we want to construct versatile “library strains” that contains many, and maybe redundant coding sequences of commonly used proteins which are dormant by default; when we need to use the function of particular already coded proteins, we just simply transform a much smaller “Minimid” containing sgDNAs targeting the desired proteins into the library strain, and then utilizing the dCas9-sgRNA complex to initiate the transcription then expression of the particular protein.  </p>
+
 
+
 
+
     <p style = "align:left"  size="2"> To show the power and practicality of the design above, our team aims to test the system by using fluorescent proteins and apply it to a sustainable dyeing strategy. Indigo, as a current widely used dye, its production, transportation and using employs many toxic and not environment-friendly chemicals. However, the enzymes that catalyze the relevant reactions are found and the biological solution seems promising. We design a strategy that not only circumvents the use of toxic reagents for indigo chemical synthesis but also removes the need for a reducing agent for dye solubilization. Our engineered E.coli will be transformed with the above proteins and become a manipulatable library for us. This project will provide a fundamental contribution to the construction of engineering bacteria and clean manufacturing. </p>
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</div>
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<div class="column full_size" >
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    <h3>Before you start</h3>
+
    <p> Please read the following pages:</p>
+
    <ul>
+
         <li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
+
        <li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
+
        <li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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    </ul>
+
</div>
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<div class="line_divider"></div>
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<div class="clear extra_space"></div>
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<div class="column full_size" >
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    <h3> Styling your wiki </h3>
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    <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
+
    <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<div class="clear extra_space"></div>
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<div class="column third_size" >
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    <h3> Uploading pictures and files </h3>
+
    <p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
+
 
+
 
+
    <p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
+
 
+
    <div class="button_link">
+
        <a href="https://2018.igem.org/Special:Upload">
+
            UPLOAD FILES
+
        </a>
+
 
     </div>
 
     </div>
 
+
    <div class="main">
</div>
+
        <div class="nav" id = "nav" >
 
+
            <ul class="navbar" style="display:inline-block;margin:0 0;">
<div class="column third_size" >
+
                    <li><a href="https://2018.igem.org/Team:SCU-China">Home</a></li>
    <h3> Wiki template information </h3>
+
                    <li><a>Project</a>
    <p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
+
                        <ul>
 
+
                            <li><a href="https://2018.igem.org/Team:SCU-China/project/overview">Overview</a></li>
</div>
+
                            <li><a href="https://2018.igem.org/Team:SCU-China/project/regulation">Precise Regulation</a></li>
 
+
                            <li><a href="https://2018.igem.org/Team:SCU-China/project/circuits">Logic Circuits</a></li>
 
+
                            <li><a href="https://2018.igem.org/Team:SCU-China/project/indigo">CRISProgrammer of Indigo</a></li>
 
+
                            <li><a href="https://2018.igem.org/Team:SCU-China/project/parts">Parts</a></li>
<div class="column third_size" >
+
                            <li><a href="https://2018.igem.org/Team:SCU-China/project/interlab">Interlab</a></li>
    <div class="highlight decoration_B_full">
+
                        </ul>
        <h3> Editing your wiki </h3>
+
                    </li>
        <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
+
                    <li><a>Modeling</a>
        <p>Use WikiTools - Edit in the black menu bar to edit this page</p>
+
                        <ul>
 
+
                            <li><a href="https://2018.igem.org/Team:SCU-China/modeling/prediction">Mismatch</a></li>
        <div class="button_link">
+
                            <li><a href="https://2018.igem.org/Team:SCU-China/modeling/algorithm">Orthogonality</a></li>
            <a href="https://2018.igem.org/wiki/index.php?title=Team:SCU-China&action=edit">
+
                        </ul>
                EDIT PAGE
+
                    </li>
             </a>
+
                    <li><a>Human practice</a>
 +
                        <ul>
 +
                         
 +
                            <li><a href="https://2018.igem.org/Team:SCU-China/practice/collaboration">Collaboration</a></li>
 +
                            <li><a href="https://2018.igem.org/Team:SCU-China/practice/meet">Meet Up</a></li>
 +
                            <li><a href="https://2018.igem.org/Team:SCU-China/practice/education">Education & Public</a></li>
 +
                        </ul>
 +
                    </li>
 +
                    <li><a>Judging</a>
 +
                        <ul>
 +
                            <li><a href="https://2018.igem.org/Team:SCU-China/judging/human">Integrated Human Practice</a></li>
 +
                            <li><a href="https://2018.igem.org/Team:SCU-China/judging/improve">Improve</a></li>
 +
                            <li><a href="https://2018.igem.org/Team:SCU-China/judging/model">Model</a></li>
 +
                            <li><a href="https://2018.igem.org/Team:SCU-China/judging/demonstrate">Demonstrate</a></li>
 +
                        </ul>
 +
                    </li>
 +
                    <li><a href="https://2018.igem.org/Team:SCU-China/attribution">Attribution</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SCU-China/safety">Safety</a></li>
 +
                    <li><a href="https://2018.igem.org/Team:SCU-China/notebook">Notebook</a></li>
 +
             </ul>
 +
        </div>
 +
        <div class="content">
 +
            <div class="block">
 +
 
 +
                <div class="phrase">Inspired by the modularization, call-and-return and do-not-reinvent-the-wheel philosophy in
 +
                    computer programming design, we come up with the idea of using the dCas9 protein to manipulate
 +
                    the expression of proteins and implement complex logic in a single E. coli cell this year. we
 +
                    want to construct versatile “library strains” that contains many, and maybe redundant coding
 +
                    sequences of commonly used proteins which are dormant by default; when we need to use the function
 +
                    of particular already coded proteins, we just simply transform a much smaller “Minimid” containing
 +
                    sgDNAs targeting the desired proteins into the library strain, and then utilizing the dCas9-sgRNA
 +
                    complex to initiate the transcription then expression of the particular protein.
 +
                </div>
 +
                <div class="phrase">To show the power and practicality of the design above, our team aims to test the system by using
 +
                    fluorescent proteins and apply it to a sustainable dyeing strategy. Indigo, as a current widely used
 +
                    dye, its production, transportation and using employs many toxic and not environment-friendly chemicals.
 +
                    However, the enzymes that catalyze the relevant reactions are found and the biological solution seems
 +
                    promising. We design a strategy that not only circumvents the use of toxic reagents for indigo chemical
 +
                    synthesis but also removes the need for a reducing agent for dye solubilization. Our engineered E.coli
 +
                    will be transformed with the above proteins and become a manipulatable library for us. This project
 +
                    will provide a fundamental contribution to the construction of engineering bacteria and clean manufacturing.
 +
                </div>
 +
            </div>
 
         </div>
 
         </div>
 
 
 
     </div>
 
     </div>
 +
    <div class="footer">SCU_China 2018 iGEM Team</div>
 
</div>
 
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    <h3>Tips</h3>
 
    <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
    <ul>
 
        <li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
        <li>Be clear about what you are doing and how you plan to do this.</li>
 
        <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
        <li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
        <li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
        <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
 
        <li>Have lots of fun! </li>
 
    </ul>
 
</div>
 
 
 
<div class="column third_size">
 
    <div class="highlight decoration_A_full">
 
        <h3>Inspiration</h3>
 
        <p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
        <ul>
 
            <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
            <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 
            <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 
            <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
            <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
            <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
        </ul>
 
    </div>
 
</div>
 
 
 
  
 
</body>
 
</body>
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Latest revision as of 03:03, 18 October 2018

Team:SCU-China - 2018

Inspired by the modularization, call-and-return and do-not-reinvent-the-wheel philosophy in computer programming design, we come up with the idea of using the dCas9 protein to manipulate the expression of proteins and implement complex logic in a single E. coli cell this year. we want to construct versatile “library strains” that contains many, and maybe redundant coding sequences of commonly used proteins which are dormant by default; when we need to use the function of particular already coded proteins, we just simply transform a much smaller “Minimid” containing sgDNAs targeting the desired proteins into the library strain, and then utilizing the dCas9-sgRNA complex to initiate the transcription then expression of the particular protein.
To show the power and practicality of the design above, our team aims to test the system by using fluorescent proteins and apply it to a sustainable dyeing strategy. Indigo, as a current widely used dye, its production, transportation and using employs many toxic and not environment-friendly chemicals. However, the enzymes that catalyze the relevant reactions are found and the biological solution seems promising. We design a strategy that not only circumvents the use of toxic reagents for indigo chemical synthesis but also removes the need for a reducing agent for dye solubilization. Our engineered E.coli will be transformed with the above proteins and become a manipulatable library for us. This project will provide a fundamental contribution to the construction of engineering bacteria and clean manufacturing.