Difference between revisions of "Team:SCU-China"

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{{SCU-China}}
 
 
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    <title>Team:SCU-China - 2018</title>
  
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<h1> Welcome to iGEM 2018! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h3>Before you start</h3>
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        <img style="width:100%;" src="https://static.igem.org/mediawiki/2018/5/52/T--SCU-China--headback.jpg" />
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<p> Please read the following pages:</p>
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<li> <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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        <div class="nav" id = "nav" >
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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            <ul class="navbar" style="display:inline-block;margin:0 0;">
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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                    <li><a href="https://2018.igem.org/Team:SCU-China">Home</a></li>
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</ul>
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                    <li><a>Project</a>
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                        <ul>
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                            <li><a href="https://2018.igem.org/Team:SCU-China/project/overview">Overview</a></li>
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                            <li><a href="https://2018.igem.org/Team:SCU-China/project/regulation">Precise Regulation</a></li>
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                            <li><a href="https://2018.igem.org/Team:SCU-China/project/circuits">Logic Circuits</a></li>
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                            <li><a href="https://2018.igem.org/Team:SCU-China/project/indigo">CRISProgrammer of Indigo</a></li>
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                            <li><a href="https://2018.igem.org/Team:SCU-China/project/parts">Parts</a></li>
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                            <li><a href="https://2018.igem.org/Team:SCU-China/project/interlab">Interlab</a></li>
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                        </ul>
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                    </li>
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                    <li><a>Modeling</a>
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                        <ul>
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                            <li><a href="https://2018.igem.org/Team:SCU-China/modeling/prediction">Mismatch</a></li>
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                            <li><a href="https://2018.igem.org/Team:SCU-China/modeling/algorithm">Orthogonality</a></li>
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                        </ul>
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                    </li>
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                    <li><a>Human practice</a>
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                        <ul>
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                            <li><a href="https://2018.igem.org/Team:SCU-China/practice/collaboration">Collaboration</a></li>
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                            <li><a href="https://2018.igem.org/Team:SCU-China/practice/meet">Meet Up</a></li>
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                            <li><a href="https://2018.igem.org/Team:SCU-China/practice/education">Education & Public</a></li>
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                        </ul>
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                    </li>
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                    <li><a>Judging</a>
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                        <ul>
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                            <li><a href="https://2018.igem.org/Team:SCU-China/judging/human">Integrated Human Practice</a></li>
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                            <li><a href="https://2018.igem.org/Team:SCU-China/judging/improve">Improve</a></li>
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                            <li><a href="https://2018.igem.org/Team:SCU-China/judging/model">Model</a></li>
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                            <li><a href="https://2018.igem.org/Team:SCU-China/judging/demonstrate">Demonstrate</a></li>
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                        </ul>
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                    </li>
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                    <li><a href="https://2018.igem.org/Team:SCU-China/attribution">Attribution</a></li>
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                    <li><a href="https://2018.igem.org/Team:SCU-China/safety">Safety</a></li>
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                    <li><a href="https://2018.igem.org/Team:SCU-China/notebook">Notebook</a></li>
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            </ul>
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        <div class="content">
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            <div class="block">
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                <div class="phrase">Inspired by the modularization, call-and-return and do-not-reinvent-the-wheel philosophy in
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                    computer programming design, we come up with the idea of using the dCas9 protein to manipulate
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                    the expression of proteins and implement complex logic in a single E. coli cell this year. we
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                    want to construct versatile “library strains” that contains many, and maybe redundant coding
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                    sequences of commonly used proteins which are dormant by default; when we need to use the function
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                    of particular already coded proteins, we just simply transform a much smaller “Minimid” containing
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                    sgDNAs targeting the desired proteins into the library strain, and then utilizing the dCas9-sgRNA
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                    complex to initiate the transcription then expression of the particular protein.
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                </div>
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                <div class="phrase">To show the power and practicality of the design above, our team aims to test the system by using
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                    fluorescent proteins and apply it to a sustainable dyeing strategy. Indigo, as a current widely used
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                    dye, its production, transportation and using employs many toxic and not environment-friendly chemicals.
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                    However, the enzymes that catalyze the relevant reactions are found and the biological solution seems
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                    promising. We design a strategy that not only circumvents the use of toxic reagents for indigo chemical
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                    synthesis but also removes the need for a reducing agent for dye solubilization. Our engineered E.coli
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                    will be transformed with the above proteins and become a manipulatable library for us. This project
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                    will provide a fundamental contribution to the construction of engineering bacteria and clean manufacturing.
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                </div>
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            </div>
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        </div>
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    <div class="footer">SCU_China 2018 iGEM Team</div>
 
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<h3> Styling your wiki </h3>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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<h3>Inspiration</h3>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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Latest revision as of 03:03, 18 October 2018

Team:SCU-China - 2018

Inspired by the modularization, call-and-return and do-not-reinvent-the-wheel philosophy in computer programming design, we come up with the idea of using the dCas9 protein to manipulate the expression of proteins and implement complex logic in a single E. coli cell this year. we want to construct versatile “library strains” that contains many, and maybe redundant coding sequences of commonly used proteins which are dormant by default; when we need to use the function of particular already coded proteins, we just simply transform a much smaller “Minimid” containing sgDNAs targeting the desired proteins into the library strain, and then utilizing the dCas9-sgRNA complex to initiate the transcription then expression of the particular protein.
To show the power and practicality of the design above, our team aims to test the system by using fluorescent proteins and apply it to a sustainable dyeing strategy. Indigo, as a current widely used dye, its production, transportation and using employs many toxic and not environment-friendly chemicals. However, the enzymes that catalyze the relevant reactions are found and the biological solution seems promising. We design a strategy that not only circumvents the use of toxic reagents for indigo chemical synthesis but also removes the need for a reducing agent for dye solubilization. Our engineered E.coli will be transformed with the above proteins and become a manipulatable library for us. This project will provide a fundamental contribution to the construction of engineering bacteria and clean manufacturing.
SCU_China 2018 iGEM Team