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| <ul> | | <ul> |
| <li><a href="https://2018.igem.org/Team:SCU-China/project/overview">Overview</a></li> | | <li><a href="https://2018.igem.org/Team:SCU-China/project/overview">Overview</a></li> |
− | <li><a href="https://2018.igem.org/Team:SCU-China/project/interlab">Interlab</a></li>
| |
| <li><a href="https://2018.igem.org/Team:SCU-China/project/regulation">Precise Regulation</a></li> | | <li><a href="https://2018.igem.org/Team:SCU-China/project/regulation">Precise Regulation</a></li> |
| <li><a href="https://2018.igem.org/Team:SCU-China/project/circuits">Logic Circuits</a></li> | | <li><a href="https://2018.igem.org/Team:SCU-China/project/circuits">Logic Circuits</a></li> |
| <li><a href="https://2018.igem.org/Team:SCU-China/project/indigo">CRISProgrammer of Indigo</a></li> | | <li><a href="https://2018.igem.org/Team:SCU-China/project/indigo">CRISProgrammer of Indigo</a></li> |
| <li><a href="https://2018.igem.org/Team:SCU-China/project/parts">Parts</a></li> | | <li><a href="https://2018.igem.org/Team:SCU-China/project/parts">Parts</a></li> |
| + | <li><a href="https://2018.igem.org/Team:SCU-China/project/interlab">Interlab</a></li> |
| </ul> | | </ul> |
| </li> | | </li> |
| <li><a>Modeling</a> | | <li><a>Modeling</a> |
| <ul> | | <ul> |
− | <li><a href="https://2018.igem.org/Team:SCU-China/modeling/prediction">Mismatch</a></li> | + | <li><a href="https://2018.igem.org/Team:SCU-China/modeling/prediction">Off Target Prediction</a></li> |
− | <li><a href="https://2018.igem.org/Team:SCU-China/modeling/algorithm">Orthogonality</a></li> | + | <li><a href="https://2018.igem.org/Team:SCU-China/modeling/algorithm">Orthogonal promoter construction Algorithm</a></li> |
| </ul> | | </ul> |
| </li> | | </li> |
| <li><a>Human practice</a> | | <li><a>Human practice</a> |
| <ul> | | <ul> |
− | <li><a href="https://2018.igem.org/Team:SCU-China/practice/hp">Integrated Human Practice</a></li>
| |
| <li><a href="https://2018.igem.org/Team:SCU-China/practice/collaboration">Collaboration</a></li> | | <li><a href="https://2018.igem.org/Team:SCU-China/practice/collaboration">Collaboration</a></li> |
| <li><a href="https://2018.igem.org/Team:SCU-China/practice/meet">Meet Up</a></li> | | <li><a href="https://2018.igem.org/Team:SCU-China/practice/meet">Meet Up</a></li> |
| <li><a href="https://2018.igem.org/Team:SCU-China/practice/education">Education & Public</a></li> | | <li><a href="https://2018.igem.org/Team:SCU-China/practice/education">Education & Public</a></li> |
| + | </ul> |
| + | </li> |
| + | <li><a>Judging</a> |
| + | <ul> |
| + | <li><a href="https://2018.igem.org/Team:SCU-China/judging/human">Integrated Human Practice</a></li> |
| + | <li><a href="https://2018.igem.org/Team:SCU-China/judging/improve">Improve</a></li> |
| + | <li><a href="https://2018.igem.org/Team:SCU-China/judging/model">Model</a></li> |
| + | <li><a href="https://2018.igem.org/Team:SCU-China/judging/demonstrate">Demonstrate</a></li> |
| </ul> | | </ul> |
| </li> | | </li> |
| <li><a href="https://2018.igem.org/Team:SCU-China/attribution">Attribution</a></li> | | <li><a href="https://2018.igem.org/Team:SCU-China/attribution">Attribution</a></li> |
− | <li><a href="https://2018.igem.org/Team:SCU-China/about">About us</a></li>
| |
| <li><a href="https://2018.igem.org/Team:SCU-China/safety">Safety</a></li> | | <li><a href="https://2018.igem.org/Team:SCU-China/safety">Safety</a></li> |
| <li><a href="https://2018.igem.org/Team:SCU-China/notebook">Notebook</a></li> | | <li><a href="https://2018.igem.org/Team:SCU-China/notebook">Notebook</a></li> |
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| </div> | | </div> |
| <div class="head" > | | <div class="head" > |
− | <img src="https://static.igem.org/mediawiki/2018/6/6c/T--SCU-China--bg.jpg" style="width:100%;"> | + | <img src="https://static.igem.org/mediawiki/2018/5/52/T--SCU-China--headback.jpg" style="width:100%;"> |
| <div class="headline sides"> | | <div class="headline sides"> |
− | <div>Integrated Human Practice</div>
| + | |
| </div> | | </div> |
| </div> | | </div> |
| <div class="content"> | | <div class="content"> |
| <div class="main"> | | <div class="main"> |
| + | <div class="sidebar" id="sidebar"> |
| + | <ul> |
| + | <li><a href="#interview">Interview</a></li> |
| + | <li><a href="#ccic">CCiC 2018</a></li> |
| + | <li><a href="#iswu">iSWU</a></li> |
| + | </ul> |
| + | </div> |
| <div class="maincontent"> | | <div class="maincontent"> |
− | <div class="phrase"> | + | <div class="phrase" id="interview"> |
− | <div class="subtitle">2018 Conference of China iGEMer Community (CCiC)</div> | + | </br></br> |
| + | <div class="subtitle">01. Interview with synthetic biology experts</div> |
| + | </br></br><hr style="height:1px;border:none;border-top:1px solid #555555;" /></br> |
| + | <div class="text">We attended 2018 CCIC meeting, interviewed with our consultants Dr. Yunzi Luo and Dr. Fei Huang, through which activities, we got a lot of profound and useful advice to optimize my project. |
| + | <br /> |
| + | <em>Dr. Yunzi Luo</em> is an expert in synthetic biology and also a consultant for SCU-WestChina in 2017 iGEM. Her suggestions are as follow: |
| + | |
| + | <ul> |
| + | <li>It is plausible to regulate gene expression by changing the extent of mismatch between sgRNA and target DNA, but up to now, there is no related model to measure this process.</li> |
| + | <li>Owing to the genome differences between E.coli DH5α and BL21 strains, we need to pay attention to sgRNA expression level in those two strains. </li> |
| + | <li>In order to make different plasmids compatible in one bacterium, we had better use the special strain with pRSF DUET and use plasmids with the different origin of replication (ORI)</li> |
| + | <li>Using antibiotic resistance genes would be more convenient than using GFP and RFP genes to verify our logic circuit. Because it is easy to measure the survival rates when using antibiotic resistance genes to measure the CRISPRi efficiency, while it is hard to measure GFP and RFP relative expression ratio to demonstrate the efficiency.</li> |
| + | <li>Softwares, such as Chopchop, can be used to predict the 3D structure of sgRNA.</li> |
| + | </ul> |
| + | <em>Dr. Fei Huang</em>is a biologist in a biological company and has been our team’s consultant for a longtime. He showed great interest in our project and gave useful advice, as follows: |
| + | <br /> |
| + | <ul> |
| + | <li>chemical synthesis of indigo in the industry currently has caused great environmental pollution, while biological synthesis of indigo is pretty much more environmentally friendly and is promising to be used in the industry for mass production. </li> |
| + | <li>At present, regulation of multiple genes’ expression in industry is hard to fulfill. However, CRISPRProgrammer provide a insightful and innovative strategy to solve this problem because the regulation of multiple genes’ expression can be achieved by only transforming a small plasmid. </li> |
| + | <li>It is easy for bacteria to lose plasmids because of the unsynchronized rate of plasmid replication, in other words, big plasmids are replicated more slowly that that of small plasmids, as a result, smalls plasmids have more copies and thus more likely to be maintained in one bacterium. Therefore, we had better insert big genes into E.coli genome in order to balance the plasmids length and give those plasmids comparative replication rates.</li> |
| + | </ul> |
| + | <div class="imgshow"><img style="width:320px" src="https://static.igem.org/mediawiki/2018/2/2b/T--SCU-China--mu111.jpg" /><br />Figure 1. interview with Dr. Fei Huang (in the middle)</div> |
| + | </div> |
| + | </div> |
| + | <div class="phrase" id="ccic"> |
| + | </br></br> |
| + | <div class="subtitle">02. 2018 Conference of China iGEMer Community (CCiC)</div> |
| + | </br></br><hr style="height:1px;border:none;border-top:1px solid #555555;" /></br> |
| <br /> | | <br /> |
− | We participated in the 5th Conference of China iGEMer Community (CCiC) held by ShanghaiTech University, | + | We participated in the 5th Conference of China iGEMer Community (CCiC) held by ShanghaiTech University, ShanghaiTech-iGEM. Through discussions with other iGEM teams from top universities across the country and communications with excellent iGEM seniors, we have gained a lot of suggestions about our project and innovative ideas about Human Practice. Furthermore, we also gave other teams feedback after listening to their presentations. |
− | ShanghaiTech-iGEM. Through discussions with other iGEM teams from top universities across the country
| + | |
− | and communications with excellent iGEM seniors, we have gained a lot of suggestions about our project
| + | |
− | and innovative ideas about Human Practice. Furthermore, we also gave other teams feedback after listening
| + | |
− | to their presentations.
| + | |
− | <div class="imgshow"><img style="width:320px" src="https://static.igem.org/mediawiki/2018/c/cc/T--SCU-China--mu1.png"/></div>
| + | |
| <br /> | | <br /> |
− | CCiC is an excellent platform for iGEMers to learn from each other. Every year, iGEMers from many | + | CCiC is an excellent platform for iGEMers to learn from each other. Every year, iGEMers from many colleges gather to introduce their projects, discuss their problems, ask questions and make suggestions to other teams, aiming to improve their project. Moreover, we acquired more information about After iGEM and the frontier of synthetic biology. |
− | colleges gather to introduce their projects, discuss their problems, ask questions and make suggestions
| + | <div class="imgshow"><img style="width:320px" src="https://static.igem.org/mediawiki/2018/c/cf/T--SCU-China--mu2.jpg" /><img style="width:320px" src="https://static.igem.org/mediawiki/2018/a/a2/T--SCU-China--mu3.jpg" /><br />Figure 2. CCiC</div> |
− | to other teams, aiming to improve their project. Moreover, we acquired more information about After
| + | |
− | iGEM and the frontier of synthetic biology.
| + | |
− | <div class="imgshow"><img style="width:320px" src="https://static.igem.org/mediawiki/2018/c/cf/T--SCU-China--mu2.jpg" /><img style="width:320px" src="https://static.igem.org/mediawiki/2018/a/a2/T--SCU-China--mu3.jpg" /></div> | + | |
| <br /> | | <br /> |
− | We showed the audience and judges our project, CRISProgrammer, and then several teams expressed their | + | We showed the audience and judges our project, CRISProgrammer, and then several teams expressed their interests in our design. During the questioning session and poster session, they also communicated with us. In the panel session, Dr. Xuan Wang asked us more detailed questions on the progress of our experiment and encouraged us. |
− | interests in our design. During the questioning session and poster session, they also communicated
| + | |
− | with us. In the panel session, Dr. Xuan Wang asked us more detailed questions on the progress of our
| + | |
− | experiment and encouraged us.
| + | |
− | <div class="imgshow"><img style="width:320px" src="https://static.igem.org/mediawiki/2018/1/17/T--SCU-China--mu4.jpg" /><img style="width:320px" src="https://static.igem.org/mediawiki/2018/6/61/T--SCU-China--mu5.jpg" /></div>
| + | |
| <br /> | | <br /> |
− | In the poster session, we introduced our ideas to other teams. Other teams also shared their experience | + | In the poster session, we introduced our ideas to other teams. Other teams also shared their experience related to us. |
− | related to us.
| + | |
− | <div class="imgshow"><img style="width:320px" src="https://static.igem.org/mediawiki/2018/6/6e/T--SCU-China--mu6.jpg" /></div>
| + | |
| <br /> | | <br /> |
− | We also invited Dr. Haotian Guo to give some advice. We introduced our subject and designing philosophy | + | We also invited Dr. Haotian Guo to give some advice. We introduced our subject and designing philosophy to him and hoped that he would comment and guide our topic. Dr. Haotian Guo put forward questions about the relationship between the amount of gRNA and the efficiency of dCas9 protein, which made us think deeply. In the communication with Dr. Xuan Wang, he expressed appreciation for our subject and suggested that we should focus on the achievement of the logic, pointing out that our ideas may solve "the key issue in the biological laboratory", and encourage us to continue to do this. |
− | to him and hoped that he would comment and guide our topic. Dr. Haotian Guo put forward questions about
| + | <div class="imgshow"><img style="width:320px" src="https://static.igem.org/mediawiki/2018/6/6e/T--SCU-China--mu6.jpg" /><br />Figure 3. CCiC</div> |
− | the relationship between the amount of gRNA and the efficiency of dCas9 protein, which made us think deeply.
| + | |
− | In the communication with Dr. Xuan Wang, he expressed appreciation for our subject and suggested that
| + | |
− | we should focus on the achievement of the logic, pointing out that our ideas may solve "the key issue
| + | |
− | in the biological laboratory", and encourage us to continue to do this.
| + | |
− | <div class="imgshow"><img style="width:320px" src="https://static.igem.org/mediawiki/2018/d/d5/T--SCU-China--mu7.jpg" /></div> | + | |
| <br /> | | <br /> |
− | Students from Peking University and Tsinghua University came to our poster area to learn more about | + | Students from Peking University and Tsinghua University came to our poster area to learn more about our topics, during which they provided us with valuable suggestions. We also asked them some questions in their poster areas and gave our opinion. Meanwhile we introduced our "Competition Guide about How to Hold a Synthetic Biology Competition in College" to some of them, which won unanimous praise from everyone. We believed that Human Practice could integrate more elements, attract more teams and create more innovative forms in the future. |
− | our topics, during which they provided us with valuable suggestions. We also asked them some questions
| + | |
− | in their poster areas and gave our opinion. Meanwhile we introduced our "Competition Guide about
| + | |
− | How to Hold a Synthetic Biology Competition in College" to some of them, which won unanimous praise
| + | |
− | from everyone. We believed that Human Practice could integrate more elements, attract more teams and
| + | |
− | create more innovative forms in the future.
| + | |
− | <div class="imgshow"><img style="width:280px" src="https://static.igem.org/mediawiki/2018/e/ec/T--SCU-China--mu8.jpg" /></div>
| + | |
| </div> | | </div> |
− | <br />
| + | <div class="phrase" id="iswu"> |
− | <br />
| + | </br></br> |
− | <div class="phrase"> | + | <div class="subtitle">03. China iGEM Southwest Union (iSWU)</div> |
− | <div class="subtitle">China iGEM Southwest Union (iSWU)</div> | + | </br></br><hr style="height:1px;border:none;border-top:1px solid #555555;" /></br> |
| <br /> | | <br /> |
| On the basis of iGEM teams in southwestern China, the China iGEM Southwest Union(iSWU), which was | | On the basis of iGEM teams in southwestern China, the China iGEM Southwest Union(iSWU), which was |
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| This year, the union was composed of iGEMers from University of Sichuan Agricultural University(SiCAU-China), | | This year, the union was composed of iGEMers from University of Sichuan Agricultural University(SiCAU-China), |
| University of Sichuan (SCU-China ) and University of Electronic Science and Technology (UEST-China & UEST-software). | | University of Sichuan (SCU-China ) and University of Electronic Science and Technology (UEST-China & UEST-software). |
− | <div class="imgshow"><img style="width:780px" src="https://static.igem.org/mediawiki/2018/3/31/T--SCU-China--mu9.jpg" /><p>Fig 1</p></div> | + | <div class="imgshow"><img style="width:320px" style="width:780px" src="https://static.igem.org/mediawiki/2018/3/31/T--SCU-China--mu9.jpg" /><br />Figure 4. iSWU</div> |
| <br /> | | <br /> |
| iSWU meet-up was held ahead of experiments this year which aimed at enhancing allied communication | | iSWU meet-up was held ahead of experiments this year which aimed at enhancing allied communication |
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| we knew the process and dilemma of other projects while discovered the shortage of our project. | | we knew the process and dilemma of other projects while discovered the shortage of our project. |
| In summarization, we not only optimize our project but also reach a win-win result. | | In summarization, we not only optimize our project but also reach a win-win result. |
− | <div class="imgshow"><img style="width:780px" src="https://static.igem.org/mediawiki/2018/4/41/T--SCU-China--mu10.jpg" /><p>Fig 2</p></div> | + | <div class="imgshow"><img style="width:320px" src="https://static.igem.org/mediawiki/2018/4/41/T--SCU-China--mu10.jpg" /><p style="text-align: center;">Figure 5</p></div> |
| <br /> | | <br /> |
| In this meet-up, team “UEST-China” showed the project “Decomposing straw and producing clean energy | | In this meet-up, team “UEST-China” showed the project “Decomposing straw and producing clean energy |
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| the experimental project that could avoid repetitively useless work in a further experiment. Furthermore, | | the experimental project that could avoid repetitively useless work in a further experiment. Furthermore, |
| communication and cooperation were existing as well as in the meet-up. | | communication and cooperation were existing as well as in the meet-up. |
− | <div class="imgshow"><img style="width:780px" src="https://static.igem.org/mediawiki/2018/0/01/T--SCU-China--mu11.jpg" /><p>Fig 3</p></div> | + | <div class="imgshow"><img style="width:320px" src="https://static.igem.org/mediawiki/2018/0/01/T--SCU-China--mu11.jpg" /><p style="text-align: center;">Figure 6</p></div> |
| <br /> | | <br /> |
| Shishi middle school and Huayang middle school couldn’t attend the meet-up because of the time arrangement. | | Shishi middle school and Huayang middle school couldn’t attend the meet-up because of the time arrangement. |
| After this meeting, we also explained the lab safety to them. They also sought support on the experimental instrument. | | After this meeting, we also explained the lab safety to them. They also sought support on the experimental instrument. |
− | <div class="imgshow"><img style="width:780px" src="https://static.igem.org/mediawiki/2018/9/91/T--SCU-China--mu12.jpg" /><p>Fig 4</p></div> | + | |
| + | |
| + | <div class="imgshow"><img style="width:320px" src="https://static.igem.org/mediawiki/2018/9/91/T--SCU-China--mu12.jpg" /><p style="text-align: center;">Figure 7. iSWU</p></div> |
| <br /> | | <br /> |
| The iSWU created a communication platform and cooperative opportunity for iGEMers in Southwest. | | The iSWU created a communication platform and cooperative opportunity for iGEMers in Southwest. |
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| </div> | | </div> |
| </div> | | </div> |
| + | <div style="clear:both;"></div> |
| </div> | | </div> |
| </div> | | </div> |
− | <div class="footer">SCU_China 2018 iGEM Team</div>
| |
| | | |
− | </div>
| |
| | | |
| + | </div> |
| + | <div class="footer">SCU_China 2018 iGEM Team</div> |
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