|
|
(24 intermediate revisions by 8 users not shown) |
Line 1: |
Line 1: |
| {{Marburg}} | | {{Marburg}} |
| <html> | | <html> |
| + | <div class="titleWrapper"><div class="titleBackground" style="background-image:url(https://static.igem.org/mediawiki/2018/2/28/T--Marburg--header_experiments.jpg)"></div><div class="title">Experiments</div></div> |
| + | <article> |
| + | <style> |
| + | .imageContainer3x3 { |
| + | display: flex; |
| + | flex-wrap: wrap; |
| + | justify-content: space-around; |
| + | align-items: center; |
| + | } |
| + | .imageContainer3x3 div { |
| + | width: 30%; |
| + | margin-bottom: 2%; |
| + | text-align:center; |
| + | } |
| + | .imageContainer3x3 div img { |
| + | width: 100%; |
| + | } |
| + | .imageContainer2x2 { |
| + | display: flex; |
| + | flex-wrap: wrap; |
| + | justify-content: space-around; |
| + | align-items: center; |
| + | } |
| + | .imageContainer2x2 div { |
| + | width: 50%; |
| + | margin-bottom: 2%; |
| + | text-align:center; |
| + | } |
| + | .imageContainer2x2 div img { |
| + | width: 100%; |
| + | } |
| | | |
− | <div class="column full_size"> | + | </style> |
| | | |
− | <h1>Experiments</h1>
| |
− | <p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.</p>
| |
| | | |
| + | |
| + | |
| + | <body> |
| <p> | | <p> |
− | Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.
| + | <i>We keep moving forward, opening new doors, and doing new things, <br> |
− | </p> | + | because we're curious and curiosity keeps leading us down new paths.</i> <br> |
| + | <b>-- Walt Disney</b><br> |
| + | <br> |
| + | <br> |
| + | <br> |
| | | |
− | </div>
| |
| | | |
| + | </p> |
| + | |
| | | |
| | | |
− | <div class="column two_thirds_size"> | + | <div class="skipTarget" skipname="Labbooks"></div> |
− | <h3>What should this page contain?</h3> | + | <h2>Labbooks</h2> |
| + | <hr> |
| + | |
| + | <style> |
| + | article ul li { |
| + | font-size: 0.8em; |
| + | padding:5px; |
| + | } |
| + | </style> |
| + | |
| <ul> | | <ul> |
− | <li> Protocols </li> | + | <li><a href="https://static.igem.org/mediawiki/2018/9/97/T--Marburg--Labbook_Sequencing.pdf |
− | <li> Experiments </li> | + | " target="blank><abbr title="Subgroup Part Collection - Sequencing"> Subgroup Part Collection - Sequencing </abbr></a></li> |
− | <li> Documentation of the development of your project </li> | + | |
− | </ul>
| + | |
| | | |
− | </div> | + | </ul> |
| + | <ul> |
| + | <li><a href="https://static.igem.org/mediawiki/2018/9/97/T--Marburg--Labbook_Characterization.pdf |
| + | " target="blank><abbr title="Subgroup Part Collection - Characterization"> Subgroup Part Collection - Characterization </abbr></a></li> |
| | | |
− | <div class="column third_size">
| + | </ul> |
− | <div class="highlight decoration_A_full">
| + | |
− | <h3>Inspiration</h3>
| + | |
| <ul> | | <ul> |
− | <li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li> | + | <li><a href="https://static.igem.org/mediawiki/2018/9/97/T--Marburg--Labbook_Cloning_LVL1.pdf |
− | <li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
| + | " target="blank><abbr title="Subgroup Part Collection - Cloning_LVL1"> Subgroup Part Collection - Cloning_LVL1 </abbr></a></li> |
− | <li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
| + | |
− | </ul>
| + | |
− | </div>
| + | |
− | </div>
| + | |
| | | |
| + | </ul><ul> |
| + | <li><a href="https://static.igem.org/mediawiki/2018/9/97/T--Marburg--Labbook_Cloning_Entry_Vectors.pdf |
| + | " target="blank><abbr title="Subgroup Part Collection - Cloning_Entry_Vectors"> Subgroup Part Collection - Cloning_Entry_Vectors </abbr></a></li> |
| | | |
− | <div class="clear"></div> | + | </ul> |
| + | <ul> |
| + | <li><a href="https://static.igem.org/mediawiki/2018/5/57/T--MArburg--Labbookgeneral.pdf |
| + | " target="blank><abbr title="Subgroup Strain Engineering - General Experiments"> Subgroup Strain Engineering - General Experiments</abbr></a></li> |
| + | </ul> |
| + | <ul> |
| + | <li><a href="https://static.igem.org/mediawiki/2018/1/10/T--Marburg--LabbookBauersachs.pdf |
| + | " target="blank><abbr title="Subgroup Strain Engineering - Integration"> Subgroup Strain Engineering - Integration</abbr></a></li> |
| + | </ul> |
| + | <ul> |
| + | <li><a href="https://static.igem.org/mediawiki/2018/d/dd/T--Marburg--Labbookdns.pdf |
| + | " target="blank><abbr title="Subgroup Strain Engineering - dns experiments"> Subgroup Strain Engineering - <i>dns</i> experiments </abbr></a></li> |
| + | </ul> |
| + | <ul> |
| + | <li><a href="https://static.igem.org/mediawiki/2018/2/2c/T--Marburg--Labbookcloningstrain.pdf |
| + | " target="blank><abbr title="Subgroup Strain Engineering - VibriClone"> Subgroup Strain Engineering - VibriClone </abbr></a></li> |
| + | </ul> |
| + | <ul> |
| + | <li><a href="https://static.igem.org/mediawiki/2018/7/78/T--Marburg--Labbookproteinexpression.pdf |
| + | " target="blank><abbr title="Subgroup Strain Engineering - VibriExpress"> Subgroup Strain Engineering - VibriExpress </abbr></a></li> |
| + | </ul> |
| | | |
| + | <ul> |
| + | <li><a href="https://static.igem.org/mediawiki/2018/0/0e/T--Marburg--ProteinInteraction.pdf |
| + | " target="blank><abbr title="Subgroup Strain Engineering - VibriInteract"> Subgroup Strain Engineering - VibriInteract </abbr></a></li> |
| + | </ul> |
| + | <ul> |
| + | <li><a href="https://static.igem.org/mediawiki/2018/c/c9/T--Marburg--Subgroup_Metabolic_Engineering_-_pathway_assembly.pdf |
| + | " target="blank><abbr title="Subgroup Metabolic Engineering - Pathway Assembly"> Subgroup Metabolic Engineering - Pathway Assembly </abbr></a></li> |
| + | </ul> |
| + | |
| + | <ul> |
| + | <li><a href="https://static.igem.org/mediawiki/2018/3/32/T--Marburg--Subgroup_Metabolic_Engineering-Sensor.pdf" target="blank><abbr title="Subgroup Metabolic Engineering - Sensor"> Subgroup Metabolic Engineering - Biosensor </abbr></a></li> |
| + | </ul> |
| + | |
| + | |
| + | |
| + | |
| + | |
| + | <div class="skipTarget" skipname="Protocols"></div> |
| + | <h2>Protocols</h2> |
| + | <hr> |
| + | |
| + | <style> |
| + | article ul li { |
| + | font-size: 0.8em; |
| + | padding:5px; |
| + | } |
| + | </style> |
| + | <ul> |
| + | <li><a href="https://static.igem.org/mediawiki/2018/4/4f/T--Marburg--Bradford_Assay.pdf"><abbr title="Bradford Assay"> Bradford Assay</abbr></a></li> |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/1/17/T--Marburg--Cell_Disruption.pdf"><abbr title="Cell Disruption"> Cell Disruption</abbr></a></li> |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/7/7d/T--Marburg--Enzyme_Activity_Assay.pdf"><abbr title="Enzyme Activity Assay"> Enzyme Activity Assay</abbr></a></li> |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/3/3a/T--Marburg--pH_Tolerance_Assay.pdf"><abbr title="pH Tolerance Assay"> pH Tolerance Assay</abbr></a></li> |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/7/79/T--Marburg--Protein_Expression.pdf"><abbr title="Protein Overexpression"> Protein Overexpression</abbr></a></li> |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/5/5f/T--Marburg--SDS_PAGE.pdf"><abbr title="SDS-PAGE"> SDS-PAGE</abbr></a></li> |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/d/d6/T--Marburg--Synthesis_Of_Acetyl-CoA_and_Malonyl-CoA.pdf"><abbr title="Synthesis of Acetyl-CoA and Malonyl-CoA"> Synthesis of Acetyl-CoA and Malonyl-CoA</abbr></a></li> |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/c/cb/T--Marburg--LC-MS.pdf"><abbr title="Detection of 3-hydroxypropionic acid via LC-MS"> Detection of 3-hydroxypropionic acid via LC-MS</abbr></a></li> |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/5/5f/T--Marburg--Platereader.pdf"><abbr title="Workflow for platereader measurements"> Workflow for platereader measurements</abbr></a></li> |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/2/2d/T--Marburg--tDNAisollation.pdf"><abbr title="Isolation of bacterial total DNA"> Isolation of bacterial total DNA</abbr></a></li> |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/5/5d/T--Marburg--qPCRprotocol.pdf"><abbr title="qPCR protocol"> qPCR protocol</abbr></a></li> |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/b/b2/T--Marburg--ColonyPCR.pdf"><abbr title="colony PCR"> Colony PCR</abbr></a></li> |
| + | |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/c/c5/T--Marburg--Gibson-cloning.pdf"><abbr title="Gibson Cloning"> Gibson Cloning</abbr></a></li> |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/6/6c/T--Marburg--Golden_Gate_LVL0.pdf"><abbr title="Golden_Gate_Lvl0"> Golden_Gate_Lvl0</abbr></a></li> |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/0/01/T--Marburg--Golden_Gate_Lvl1.pdf"><abbr title="Golden_Gate_Lvl1"> Golden_Gate_Lvl1</abbr></a></li> |
| + | |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/1/16/T--Marburg--Golden_Gate_Lvl2.pdf"><abbr title="Golden_Gate_Lvl2"> Golden_Gate_Lvl2</abbr></a></li> |
| + | |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/b/b8/T--Marburg--Miniprep.pdf"><abbr title="Miniprep"> Miniprep</abbr></a></li> |
| + | |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/d/d5/T--Marburg--PCR_purification.pdf"><abbr title="PCRpurification"> PCRpurification</abbr></a></li> |
| + | |
| + | |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/5/5f/T--Marburg--Q5polymerase_PCR.pdf"><abbr title="Q5_polymerase_PCR"> Q5_polymerase_PCR</abbr></a></li> |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/b/b2/T--Marburg--Glycerol_Stock.pdf"><abbr title="Glycerol_Stock_Preparation"> Glycerol_Stock_Preparation</abbr></a></li> |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/6/61/T--Marburg--Q-PCR-Protocoll.pdf"><abbr title="QPCR-Protocol"> QPCR-Protocol</abbr></a></li> |
| + | |
| + | |
| + | <li><a href="https://2018.igem.org/File:T--Marburg--Subgroup_Metabolic_Engineering_-_pathway_assembly.pdf"><abbr title="Pathway_Assembly">Pathway_Assembly</abbr></a></li> |
| + | |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/2/2d/T--Marburg--tDNAisollation.pdf"><abbr title="Genomic_DNA_Isolation">Genomic_DNA_Isolation</abbr></a></li> |
| + | |
| + | <li><a href="https://static.igem.org/mediawiki/2018/2/22/T--Marburg--FusionPCR.pdf"><abbr title="Genomic_DNA_Isolation">Fusion PCR</abbr></a></li> |
| + | |
| + | |
| + | </ul> |
| + | </body> |
| | | |
| | | |
| | | |
| + | </article> |
| </html> | | </html> |