Difference between revisions of "Team:IIT Delhi/Description"

(Prototype team page)
 
 
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{{IIT_Delhi}}
 
 
<html>
 
<html>
 +
 
 +
   
 +
<link rel="stylesheet" type="text/css" href="wickedcss.min.css">
 +
<head>
 +
  <link href='https://fonts.googleapis.com/css?family=Boogaloo' rel='stylesheet'>
 +
<link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css" >
 +
<link href="https://fonts.googleapis.com/css?family=PT+Sans" rel="stylesheet">
 +
<link href="https://fonts.googleapis.com/css?family=Roboto" rel="stylesheet">
 +
<link href="https://fonts.googleapis.com/css?family=Lato:900" rel="stylesheet">
  
 +
<meta name="viewport" content="width=device-width, initial-scale=1">   
 +
<style>
 +
body, html {
 +
  width: 100%;
 +
  height: 100%;
 +
  margin: 0;
 +
  font: 400 15px/1.8 "Lato", sans-serif;
 +
  color: #777;
 +
}
  
 +
.bgimg-1, .bgimg-2, .bgimg-3, .bgimg-5, .bgimg-4 {
 +
  position: relative;
 +
  opacity: 0.65;
 +
  background-attachment: fixed;
 +
  background-position: center;
 +
  background-repeat: no-repeat;F
 +
  background-size: cover;
  
−
<div class="column full_size">
+
}
−
<h1>Description</h1>
+
.bgimg-1 {
 +
  background-image: url("https://static.igem.org/mediawiki/2018/e/e7/T--IIT_Delhi--InterlabPlate.jpeg");
 +
  min-height: 600px;
 +
}
  
−
<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
+
.bgimg-2 {
 +
  background-image: url("https://static.igem.org/mediawiki/2018/f/ff/T--IIT_Delhi--white.jpeg");
 +
  min-height: 100px;
 +
}
  
−
</div>
+
.bgimg-3 {
 +
  background-image: url("https://static.igem.org/mediawiki/2018/f/ff/T--IIT_Delhi--white.jpeg");
 +
  min-height: 100px;
 +
}
  
 +
.bgimg-4{
 +
background-image: url("https://static.igem.org/mediawiki/2018/9/90/T--IIT_Delhi--Dark2.png");
 +
  min-height: 300px;
  
 +
}
  
−
<div class="column two_thirds_size">
+
.bgimg-5 {
−
<h3>What should this page contain?</h3>
+
  background-image: url("https://static.igem.org/mediawiki/2018/f/ff/T--IIT_Delhi--white.jpeg");
−
<ul>
+
  min-height: 100px;
−
<li> A clear and concise description of your project.</li>
+
}
−
<li>A detailed explanation of why your team chose to work on this particular project.</li>
+
−
<li>References and sources to document your research.</li>
+
−
<li>Use illustrations and other visual resources to explain your project.</li>
+
−
</ul>
+
−
</div>
+
  
−
<div class="column third_size" >
 
−
<div class="highlight decoration_A_full">
 
−
<h3>Inspiration</h3>
 
−
<p>See how other teams have described and presented their projects: </p>
 
  
−
<ul>
+
.caption {
−
<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
+
  position: absolute;
−
<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
+
  left: 0;
−
<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
+
  top: 50%;
−
<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
+
  width: 100%;
−
</ul>
+
  text-align: center;
−
</div>
+
  color: #000;
−
</div>
+
}
  
 +
.caption span.border {
 +
  background-color: #111;
 +
  color: #fff;
 +
  padding: 18px;
 +
  font-size: 25px;
 +
  letter-spacing: 10px;
 +
}
  
 +
h3 {
 +
  letter-spacing: 5px;
 +
  text-transform: uppercase;
 +
  font: 20px "Lato", sans-serif;
 +
  color: #111;
 +
}
  
 +
/* Turn off parallax scrolling for tablets and phones */
 +
@media only screen and (max-device-width: 1024px) {
 +
    .bgimg-1, .bgimg-2, .bgimg-3, .bgimg-4, .bgimg-5 {
 +
        background-attachment: scroll;
 +
    }
 +
}
 +
</style>
 +
<style>
 +
@charset "UTF-8";
 +
@import url(font-awesome.min.css);
 +
@import url("http://fonts.googleapis.com/css?family=Lato:300,400");
 +
@import 'https://fonts.googleapis.com/css?family=Boogaloo';
 +
@import 'https://fonts.googleapis.com/css?family=Raleway';
  
−
<div class="column two_thirds_size" >
+
hr {
−
<h3>Advice on writing your Project Description</h3>
+
    margin-top: 20px;
 +
    margin-bottom: 20px;
 +
    border: 0;
 +
    border-top: 1px solid #eee;
 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
    border-width: 1px;
 +
}
 +
   
 +
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 +
color:darkblue;
 +
transform:translateY(-6px);
 +
}
 +
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 +
color:lightpink;
 +
transform:translateY(-6px);
 +
}
 +
.fx2:hover{
 +
color: skyblue;
 +
transform:translateY(-6px);
 +
}
 +
    .fxm:hover{
 +
        color: indianred;
 +
        transform: translateY(-6px);
 +
    }
 +
    .fxln:hover{
 +
        color: #0077B5;
 +
        transform: translateY(-6px);
 +
    }
  
−
<p>
 
−
We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements.
 
−
</p>
 
  
−
</div>
 
  
−
<div class="column third_size">
+
body, input, select, textarea {
−
<h3>References</h3>
+
color: #444;
−
<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
+
font-family:  Helvetica, sans-serif;
 +
font-size: 15pt;
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font-weight: 300;
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line-height: 1.65em;
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        min-width: 800px;
 +
       
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}
  
 +
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 +
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margin: 0 0 1em 0;
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 +
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 +
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 +
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background-position: center;
 +
background-repeat: no-repeat;
 +
background-size: cover ;
 +
             
 +
color: #ffffff;
 +
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 +
        height: 100%;
 +
padding: 27.5em 0em 1em;
 +
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 +
padding-bottom: 150px;
 +
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 +
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 +
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 +
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 +
    padding-bottom: 10px;
 +
   
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}
 +
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 +
/* Footer */
 +
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#footer {
 +
background: #202222;
 +
color: #ddd;
 +
padding: 1em 0em;
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}
 +
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#footer h2, #footer h3, #footer h4 {
 +
color: #ffffff;
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transition: all 0.2s ease-in-out;
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 +
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 +
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 +
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 +
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 +
border-color: #ffffff;
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 +
font-size: 0.9em;
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 +
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 +
text-align: center;
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#footer .copyright li {
 +
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 +
display: inline-block;
 +
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 +
margin-left: 1.5em;
 +
padding-left: 1.5em;
 +
}
 +
 +
#footer .copyright li:first-child {
 +
border-left: 0;
 +
margin-left: 0;
 +
padding-left: 0;
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 +
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 +
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<style>
 +
 
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 +
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 +
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 +
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    border-color: white;
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    border-image: none;
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 +
    font-size: 20px;
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    text-align: left;
 +
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 +
    float: left;
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    .fa-caret-right {
 +
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 +
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 +
.overlayved a:hover, .overlayved a:focus, .overlayved-btn:hover, .overlayved-btn2:hover {
 +
    color: darkred;
 +
}
 +
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  /*  .overlayved .closebtn {
 +
    position: absolute;
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 +
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 +
    font-size: 60px;
 +
} */
 +
   
 +
    .dropdownsakks-container {
 +
    display: none;
 +
    background-color: #262626;
 +
    padding-left: 30px;
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 +
/* ADJUSTS DEFAULT iGEM CONTENT */
 +
.logo_2017, #sideMenu, #firstHeading, #bars_item { /* HIDES SOME UNWANTED STUFF COMPLETELY */
 +
display: none;
 +
}
 +
#top_menu_14, #top_menu_inside { /* MAKES BACKGROUND OF iGEM MENU TRANSPARENT */
 +
background: none;
 +
border: none;
 +
}
 +
#globalWrapperdivi, #content { /* MAKES BACKGROUND OF MEDIAWIKI TRANSPARENT */
 +
background-color: transparent;
 +
}
 +
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 +
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 +
}
 +
body, html, #globalWrapperdivi, #bodyContent, #HQ_page {
 +
width: 100%;
 +
margin: 0;
 +
padding: 0;
 +
height: 100%
 +
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 +
width: 100%;
 +
margin: 0;
 +
margin-top: -33px;
 +
padding: 0;
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 +
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html {
 +
-webkit-font-smoothing: antialiased;
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 +
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 +
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 +
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 +
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 +
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 +
    background-attachment: fixed;
 +
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 +
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        font-family: Raleway;
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padding-top: 1em ;
 +
    padding-right: 16%;
 +
text-align: center;
 +
    overflow: hidden;
 +
    background-color: white;
 +
    font-family: Arial, Helvetica, sans-serif;
 +
    position: static;
 +
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 +
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 +
 
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 +
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 +
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 +
 
 +
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@media screen and (max-width: 850px) {
 +
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        .NameClass{display: none;}
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            display: block;
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        body, html, #globalWrapperdivi, #bodyContent, #HQ_page {
 +
 +
        margin-top: -17px;
 +
        }
 +
    }   
 +
 
 +
 +
  @media screen and (max-width: 1000px) and (min-width: 850px) {
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        .NameClass{display: none;}
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        body, html, #globalWrapperdivi, #bodyContent, #HQ_page {
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        .NameClass{display: none;}
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        .RespIcon .icon {
 +
            float:  right;
 +
            display: block;
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        body, html, #globalWrapperdivi, #bodyContent, #HQ_page {
 +
 +
        margin-top: -10px;
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 +
 
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 +
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 +
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        .NameClass{display: none;}
 +
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 +
            float:  right;
 +
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        body, html, #globalWrapperdivi, #bodyContent, #HQ_page {
 +
 +
        margin-top: -8px;
 +
        }
 +
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 +
   
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 +
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</style>
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        <link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css" >
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<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;color: black;margin: auto;width: 75%;text-align: center;">
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<br><br>With the advent of synthetic biology, a lot of synthetic circuits are designed every now and then. These circuits contain a lot of components working in harmony to regulate the gene expression. In any system, as the number of components which work together increases, the need for those components to be orthogonal also increases. The components, if interfere with the working of each other, may cause the system as a whole to fail. Such systems succumb to the noise and growth-related factors present in the biological environment. Recombinases in a biological system are adept at this job and hence, are a good basis for the formation of large-scale networks. Utilizing serine recombinases in such biological circuits provides a solution to these problems, as they have non-identical recognition sites and a particular recombinase works orthogonally to other recombinases.<br>
 +
<br>
 +
These allow us to build complex circuits with genetic modification in relatively uncomplicated configurations owing to the small size of attB and attP sites(~50bp). The attP and attB sites (and thus the integrase-att complexes) have imperfect two-fold symmetry. Hence they allow recombination in two different ways, with the sites aligned in ‘parallel’ or ‘antiparallel’ configuration. Thus, the ability to integrate/excise the DNA provides flexibility in the design of the constructs.<br><br>
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<br><br>
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We at iGEM IIT Delhi made use of serine based recombinases - Bxb1 and TP901 to develop 2  recombinase-based incoherent feed-forward loop constructs. These constructs show a strong capability for regulating and reducing the expression variability of genes being transcribed in addition to faster response time compared to transcriptional networks.
 +
These constructs can be utilized in the development of complex circuits.<br><br>
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<img src="https://static.igem.org/mediawiki/2018/a/af/T--IIT_Delhi--FirstDesign.png" style="border:3px solid #000000" width="80%"><br><br>
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        document.getElementById("globalWrapperdivi").style.overflowY = "inherit";
 +
       
 +
    var Main = document.getElementById("mainBar")
 +
        {
 +
        Main.classList.add("Fixed");
 +
        }
 +
}
 +
 +
function closeNav() {
 +
    document.getElementById("myNav").style.width = "0%";
 +
      document.getElementById("globalWrapperdivi").style.width = "100%";
 +
        document.getElementById("globalWrapperdivi").style.paddingLeft = "0%";
 +
      document.body.style.overflowX = "initial";
 +
        document.body.style.overflowY = "initial";
 +
        document.getElementById("globalWrapperdivi").style.overflowX = "inherit";
 +
        document.getElementById("globalWrapperdivi").style.overflowY = "inherit";
 +
 +
   
 +
   
 +
    var Main = document.getElementById("mainBar")
 +
        {
 +
        Main.classList.remove("Fixed");
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        }
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}
 +
 +
           
 +
           
 +
           
 +
function myFunction1() {
 +
    document.getElementById("mydropdownsakks1").classList.toggle("show");
 +
   
 +
 
 +
     
 +
    var prev2 = document.getElementById("mydropdownsakks2");
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    if (prev2.classList.contains("show"))
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      prev2.classList.remove("show");
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    var prev3 = document.getElementById("mydropdownsakks3");
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    if (prev3.classList.contains("show"))
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      prev3.classList.remove("show");
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    var prev4 = document.getElementById("mydropdownsakks4");
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    if (prev4.classList.contains("show"))
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      prev4.classList.remove("show");
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        var prev5 = document.getElementById("mydropdownsakks5");
 +
    if (prev5.classList.contains("show"))
 +
      prev5.classList.remove("show");
 +
}
 +
 +
function myFunction2() {
 +
    document.getElementById("mydropdownsakks2").classList.toggle("show");
 +
   
 +
    var prev1 = document.getElementById("mydropdownsakks1");
 +
    if (prev1.classList.contains("show"))
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      prev1.classList.remove("show");
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    var prev3 = document.getElementById("mydropdownsakks3");
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    if (prev3.classList.contains("show"))
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      prev3.classList.remove("show");
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    var prev4 = document.getElementById("mydropdownsakks4");
 +
    if (prev4.classList.contains("show"))
 +
      prev4.classList.remove("show");
 +
     
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        var prev5 = document.getElementById("mydropdownsakks5");
 +
    if (prev5.classList.contains("show"))
 +
      prev5.classList.remove("show");
 +
}
  
 +
function myFunction3() {
 +
    document.getElementById("mydropdownsakks3").classList.toggle("show");
 +
   
 +
    var prev2 = document.getElementById("mydropdownsakks2");
 +
    if (prev2.classList.contains("show"))
 +
      prev2.classList.remove("show");
 +
     
 +
    var prev1 = document.getElementById("mydropdownsakks1");
 +
    if (prev1.classList.contains("show"))
 +
      prev1.classList.remove("show");
 +
     
 +
    var prev4 = document.getElementById("mydropdownsakks4");
 +
    if (prev4.classList.contains("show"))
 +
      prev4.classList.remove("show");
 +
     
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        var prev5 = document.getElementById("mydropdownsakks5");
 +
    if (prev5.classList.contains("show"))
 +
      prev5.classList.remove("show");
 +
}
  
 +
function myFunction4() {
 +
    document.getElementById("mydropdownsakks4").classList.toggle("show");
 +
   
 +
    var prev2 = document.getElementById("mydropdownsakks2");
 +
    if (prev2.classList.contains("show"))
 +
      prev2.classList.remove("show");
 +
     
 +
    var prev3 = document.getElementById("mydropdownsakks3");
 +
    if (prev3.classList.contains("show"))
 +
      prev3.classList.remove("show");
 +
     
 +
    var prev1 = document.getElementById("mydropdownsakks1");
 +
    if (prev1.classList.contains("show"))
 +
      prev1.classList.remove("show");
 +
     
 +
        var prev5 = document.getElementById("mydropdownsakks5");
 +
    if (prev5.classList.contains("show"))
 +
      prev5.classList.remove("show");
 +
}
  
 +
function myFunction5() {
 +
    document.getElementById("mydropdownsakks5").classList.toggle("show");
 +
   
 +
    var prev2 = document.getElementById("mydropdownsakks2");
 +
    if (prev2.classList.contains("show"))
 +
      prev2.classList.remove("show");
 +
     
 +
    var prev3 = document.getElementById("mydropdownsakks3");
 +
    if (prev3.classList.contains("show"))
 +
      prev3.classList.remove("show");
 +
     
 +
    var prev4 = document.getElementById("mydropdownsakks4");
 +
    if (prev4.classList.contains("show"))
 +
      prev4.classList.remove("show");
 +
     
 +
        var prev1 = document.getElementById("mydropdownsakks1");
 +
    if (prev1.classList.contains("show"))
 +
      prev1.classList.remove("show");
 +
}
  
  
−
</html>
+
</script>
 +
       
 +
 
 +
       
 +
    </body>
 +
    </html>

Latest revision as of 03:35, 18 October 2018

iGEM IIT Delhi

Notebook
Lab Records Medal Criteria
Team
Members Attributions
Collaboration
Human Practices
Human Practices Safety Public Engagement
Modelling
Parts
Basic Parts Composite Parts
Results
Demonstrate Interlab
Project
Overview Design



With the advent of synthetic biology, a lot of synthetic circuits are designed every now and then. These circuits contain a lot of components working in harmony to regulate the gene expression. In any system, as the number of components which work together increases, the need for those components to be orthogonal also increases. The components, if interfere with the working of each other, may cause the system as a whole to fail. Such systems succumb to the noise and growth-related factors present in the biological environment. Recombinases in a biological system are adept at this job and hence, are a good basis for the formation of large-scale networks. Utilizing serine recombinases in such biological circuits provides a solution to these problems, as they have non-identical recognition sites and a particular recombinase works orthogonally to other recombinases.

These allow us to build complex circuits with genetic modification in relatively uncomplicated configurations owing to the small size of attB and attP sites(~50bp). The attP and attB sites (and thus the integrase-att complexes) have imperfect two-fold symmetry. Hence they allow recombination in two different ways, with the sites aligned in ‘parallel’ or ‘antiparallel’ configuration. Thus, the ability to integrate/excise the DNA provides flexibility in the design of the constructs.



We at iGEM IIT Delhi made use of serine based recombinases - Bxb1 and TP901 to develop 2 recombinase-based incoherent feed-forward loop constructs. These constructs show a strong capability for regulating and reducing the expression variability of genes being transcribed in addition to faster response time compared to transcriptional networks. These constructs can be utilized in the development of complex circuits.