Difference between revisions of "Team:IIT Delhi/Description"

 
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<link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css" >
 
<link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css" >
 
<link href="https://fonts.googleapis.com/css?family=PT+Sans" rel="stylesheet">
 
<link href="https://fonts.googleapis.com/css?family=PT+Sans" rel="stylesheet">
−
   
+
<link href="https://fonts.googleapis.com/css?family=Roboto" rel="stylesheet">
 +
<link href="https://fonts.googleapis.com/css?family=Lato:900" rel="stylesheet">
 +
 
 
<meta name="viewport" content="width=device-width, initial-scale=1">     
 
<meta name="viewport" content="width=device-width, initial-scale=1">     
 
<style>
 
<style>
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}
 
}
  
−
.bgimg-1, .bgimg-2, .bgimg-3 {
+
.bgimg-1, .bgimg-2, .bgimg-3, .bgimg-5, .bgimg-4 {
 
   position: relative;
 
   position: relative;
 
   opacity: 0.65;
 
   opacity: 0.65;
 
   background-attachment: fixed;
 
   background-attachment: fixed;
 
   background-position: center;
 
   background-position: center;
−
   background-repeat: no-repeat;
+
   background-repeat: no-repeat;F
 
   background-size: cover;
 
   background-size: cover;
  
 
}
 
}
 
.bgimg-1 {
 
.bgimg-1 {
−
   background-image: url("https://static.igem.org/mediawiki/2018/0/00/T--IIT_Delhi--midwall.jpeg");
+
   background-image: url("https://static.igem.org/mediawiki/2018/e/e7/T--IIT_Delhi--InterlabPlate.jpeg");
−
   min-height: 800px;
+
   min-height: 600px;
 
}
 
}
  
 
.bgimg-2 {
 
.bgimg-2 {
−
   background-image: url("https://static.igem.org/mediawiki/2018/3/38/T--IIT_Delhi--temp2.png");
+
   background-image: url("https://static.igem.org/mediawiki/2018/f/ff/T--IIT_Delhi--white.jpeg");
−
   min-height: 400px;
+
   min-height: 100px;
 
}
 
}
  
 
.bgimg-3 {
 
.bgimg-3 {
−
   background-image: url("https://static.igem.org/mediawiki/2018/0/00/T--IIT_Delhi--midwall.jpeg");
+
   background-image: url("https://static.igem.org/mediawiki/2018/f/ff/T--IIT_Delhi--white.jpeg");
−
   min-height: 400px;
+
   min-height: 100px;
 
}
 
}
 +
 +
.bgimg-4{
 +
background-image: url("https://static.igem.org/mediawiki/2018/9/90/T--IIT_Delhi--Dark2.png");
 +
  min-height: 300px;
 +
 +
}
 +
 +
.bgimg-5 {
 +
  background-image: url("https://static.igem.org/mediawiki/2018/f/ff/T--IIT_Delhi--white.jpeg");
 +
  min-height: 100px;
 +
}
 +
  
 
.caption {
 
.caption {
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/* Turn off parallax scrolling for tablets and phones */
 
/* Turn off parallax scrolling for tablets and phones */
 
@media only screen and (max-device-width: 1024px) {
 
@media only screen and (max-device-width: 1024px) {
−
     .bgimg-1, .bgimg-2, .bgimg-3 {
+
     .bgimg-1, .bgimg-2, .bgimg-3, .bgimg-4, .bgimg-5 {
 
         background-attachment: scroll;
 
         background-attachment: scroll;
 
     }
 
     }
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#banner {
 
#banner {
−
background: url("https://static.igem.org/mediawiki/2018/a/a7/T--IIT_Delhi--safety.png") ;
+
background: url("https://static.igem.org/mediawiki/2018/1/18/T--IIT_Delhi--Overview.png") ;
  
 
background-position: center;
 
background-position: center;
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   z-index: 0;
 
   z-index: 0;
 
}
 
}
 +
 
 
    
 
    
−
   
+
 
−
    @media screen and (max-width: 1300px){
+
@media screen and (max-width: 850px) {
 
         .navaman a{display: none;}
 
         .navaman a{display: none;}
 
         .RespIcon {display: block;}
 
         .RespIcon {display: block;}
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         }
 
         }
 
          
 
          
 +
        body, html, #globalWrapperdivi, #bodyContent, #HQ_page {
 +
 +
        margin-top: -17px;
 +
        }
 +
    }   
 +
 
 +
 +
  @media screen and (max-width: 1000px) and (min-width: 850px) {
 +
        .navaman a{display: none;}
 +
        .RespIcon {display: block;}
 +
        .NameClass{display: none;}
 +
        .RespIcon .icon {
 +
            float:  right;
 +
            display: block;
 +
        }
 
          
 
          
 +
        body, html, #globalWrapperdivi, #bodyContent, #HQ_page {
 +
 +
        margin-top: -15px;
 +
        }
 +
    }   
 +
 
 +
 +
  @media screen and (max-width: 1200px) and (min-width: 1000px) {
 +
        .navaman a{display: none;}
 +
        .RespIcon {display: block;}
 +
        .NameClass{display: none;}
 +
        .RespIcon .icon {
 +
            float:  right;
 +
            display: block;
 +
        }
 +
       
 +
        body, html, #globalWrapperdivi, #bodyContent, #HQ_page {
 +
 +
        margin-top: -10px;
 +
        }
 +
    }   
 +
 
 +
 +
 +
    @media screen and (max-width: 1300px) and (min-width: 1200px) {
 +
        .navaman a{display: none;}
 +
        .RespIcon {display: block;}
 +
        .NameClass{display: none;}
 +
        .RespIcon .icon {
 +
            float:  right;
 +
            display: block;
 +
        }
 +
       
 +
        body, html, #globalWrapperdivi, #bodyContent, #HQ_page {
 +
 +
        margin-top: -8px;
 +
        }
 
     }     
 
     }     
 
      
 
      
 
    
 
    
−
 
 
  
 
</style>
 
</style>
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</script>
 
</script>
 
<script>
 
<script>
−
    window.sr = ScrollReveal({reset: true});
+
  window.sr = ScrollReveal({reset: true});
 
     window.sr = ScrollReveal();
 
     window.sr = ScrollReveal();
 
     /*sr.reveal('.img1', {duration: 700});
 
     /*sr.reveal('.img1', {duration: 700});
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−
         <div class= "NameClass" style="background-color:white;"><img src="https://static.igem.org/mediawiki/2018/d/de/T--IIT_Delhi--whitelogo.png" style="height: 150px; width: 150px;display:block; padding-left: 34%;margin-top: 0px; padding-top: 10px;">
+
         <div class= "NameClass" style="background-color:white;">
 +
<a style="height: 150px; width: 150px;margin-left: 34%;margin-top: 0px; padding-top: 35px;" href="https://2018.igem.org/Team:IIT_Delhi">
 +
<img src="https://static.igem.org/mediawiki/2018/d/de/T--IIT_Delhi--whitelogo.png" style="height: 150px; width: 150px;display:inline; margin-top: 40px;"> </a>
 
              
 
              
−
       
 
 
         </div>
 
         </div>
 
        
 
        
 
       <div class="RespIcon"  style="background-color: white;">
 
       <div class="RespIcon"  style="background-color: white;">
−
      <img src="https://static.igem.org/mediawiki/2018/d/de/T--IIT_Delhi--whitelogo.png" style="height: 150px; width: 160px; display:block; padding-left: 5%;margin-top: 0px; padding-top: 10px;">
+
<a href="https://2018.igem.org/Team:IIT_Delhi" style="height: 150px; width: 150px; margin-left: 5%;margin-top: 0px; padding-top: 14px;">
−
          <a href="javascript:void(0);" style="Background-color: white;" class="icon" onclick="openNav()"> <i class="fa fa-bars"></i> </a>
+
      <img src="https://static.igem.org/mediawiki/2018/d/de/T--IIT_Delhi--whitelogo.png" style="height: 150px; width: 150px; display:inline; padding-top: 15px;">   </a>      <a href="javascript:void(0);" style="Background-color: white;" class="icon" onclick="openNav()"> <i class="fa fa-bars" style="padding-top: 100px"></i> </a>
 
       </div>
 
       </div>
 
          
 
          
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     </div>
 
     </div>
−
     <div class="dropdownsakks-content"  style="margin-top: 62px;">
+
     <div class="dropdownsakks-content"  style="margin-top: 66px;">
−
       <a href="https://2018.igem.org/Team:IIT_Delhi/Team">Lab Records</a>
+
       <a href="https://2018.igem.org/Team:IIT_Delhi/Notebook">Lab Records</a>
−
      <a href="https://2018.igem.org/Team:IIT_Delhi/Collaborations">Protocol</a>
+
                      <a href="https://igem.org/2018_Judging_Form?team=IIT_Delhi">Medal Criteria</a>
−
              <a href="https://2018.igem.org/Team:IIT_Delhi/Collaborations">Recipes</a>
+
−
                      <a href="https://2018.igem.org/Team:IIT_Delhi/Collaborations">Medal Criteria</a>
+
 
         </div>
 
         </div>
 
     </div>
 
     </div>
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     </div>
 
     </div>
−
     <div class="dropdownsakks-content"  style="margin-top: 62px;">
+
     <div class="dropdownsakks-content"  style="margin-top: 66px;">
 
       <a href="https://2018.igem.org/Team:IIT_Delhi/Team">Members</a>
 
       <a href="https://2018.igem.org/Team:IIT_Delhi/Team">Members</a>
−
       <a href="https://2018.igem.org/Team:IIT_Delhi/Collaborations">Attributions</a>
+
       <a href="https://2018.igem.org/Team:IIT_Delhi/Attributions">Attributions</a>
−
              <a href="https://2018.igem.org/Team:IIT_Delhi/Collaborations">Sponsors</a>
+
           
 
         </div>
 
         </div>
 
     </div>
 
     </div>
 
      
 
      
−
      
+
      <div class= "borderYtoX">
 +
      <a href="https://2018.igem.org/Team:IIT_Delhi/Collaborations"> Collaboration</a>
 +
     </div>
 
          
 
          
 
       <div class="dropdownsakks">
 
       <div class="dropdownsakks">
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           <a href="javascript:void(0)"> Human Practices </a>
 
           <a href="javascript:void(0)"> Human Practices </a>
 
           </div>
 
           </div>
−
     <div class="dropdownsakks-content"  style="margin-top: 62px;">
+
     <div class="dropdownsakks-content"  style="margin-top: 66px;">
 
       <a href="https://2018.igem.org/Team:IIT_Delhi/Human_Practices">Human Practices</a>
 
       <a href="https://2018.igem.org/Team:IIT_Delhi/Human_Practices">Human Practices</a>
−
      <a href="https://2018.igem.org/Team:IIT_Delhi/Public_Engagement">Integrated Practices</a>
+
   
−
           <a href="https://2018.igem.org/Team:IIT_Delhi/Public_Engagement">Safety</a>
+
           <a href="https://2018.igem.org/Team:IIT_Delhi/Safety">Safety</a>
 
               <a href="https://2018.igem.org/Team:IIT_Delhi/Public_Engagement">Public Engagement</a>
 
               <a href="https://2018.igem.org/Team:IIT_Delhi/Public_Engagement">Public Engagement</a>
 
     </div>
 
     </div>
 
   </div>  
 
   </div>  
 
       <div class= "borderYtoX">
 
       <div class= "borderYtoX">
−
       <a href="https://2018.igem.org/Team:IIT_Delhi/Safety">Modelling</a>
+
       <a href="https://2018.igem.org/Team:IIT_Delhi/Model">Modelling</a>
 
     </div>
 
     </div>
 
     <div class="dropdownsakks">
 
     <div class="dropdownsakks">
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     <a href="javascript:void(0)"> Parts </a>
 
     <a href="javascript:void(0)"> Parts </a>
 
         </div>
 
         </div>
−
     <div class="dropdownsakks-content" style="margin-top: 62px;">
+
     <div class="dropdownsakks-content" style="margin-top: 66px;">
  
 
       <a href="https://2018.igem.org/Team:IIT_Delhi/Basic_Part">Basic Parts</a>
 
       <a href="https://2018.igem.org/Team:IIT_Delhi/Basic_Part">Basic Parts</a>
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     </div>
 
     </div>
 
   </div>
 
   </div>
−
 
+
<div class="dropdownsakks">
 
         <div class= "borderYtoX">
 
         <div class= "borderYtoX">
−
   <a href="https://igem.org/2018_Judging_Form?team=IIT_Delhi" >Results</a>
+
   <a href="javascript:void(0)" >Results</a>
 
     </div>
 
     </div>
  
−
          
+
  <div class="dropdownsakks-content"  style="margin-top: 66px;">
 +
      <a href="https://2018.igem.org/Team:IIT_Delhi/Demonstrate">Demonstrate</a>
 +
      <a href="https://2018.igem.org/Team:IIT_Delhi/InterLab">Interlab</a>
 +
     
 +
    </div>
 +
 
 +
 
 +
 
 +
         </div>
 
          
 
          
 
          
 
          
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             <a href="javascript:void(0)"> Project </a>
 
             <a href="javascript:void(0)"> Project </a>
 
           </div>
 
           </div>
−
     <div class="dropdownsakks-content"  style="margin-top: 62px;">
+
     <div class="dropdownsakks-content"  style="margin-top: 66px;">
 
       <a href="https://2018.igem.org/Team:IIT_Delhi/Description">Overview</a>
 
       <a href="https://2018.igem.org/Team:IIT_Delhi/Description">Overview</a>
 
       <a href="https://2018.igem.org/Team:IIT_Delhi/Design">Design</a>
 
       <a href="https://2018.igem.org/Team:IIT_Delhi/Design">Design</a>
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     <div class="dropdownsakks-container" id="mydropdownsakks1" style= "font-style: italic; ">
 
     <div class="dropdownsakks-container" id="mydropdownsakks1" style= "font-style: italic; ">
−
     <a href="#" style="font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 0px;">Overview</a>
+
     <a href="https://2018.igem.org/Team:IIT_Delhi/Description" style="font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 0px;">Overview</a>
−
     <a href="#" style="font-size: 15px;padding: 10px; padding-top: 5px;
+
     <a href="https://2018.igem.org/Team:IIT_Delhi/Design" style="font-size: 15px;padding: 10px; padding-top: 5px;
 
                       padding-bottom: 0px;" >Design</a>
 
                       padding-bottom: 0px;" >Design</a>
 
     </div>
 
     </div>
 
      
 
      
−
        <a href="#safety" style="padding-bottom: 15px;padding-top: 15px;" >Results</a>
 
−
       
 
 
     <button class="overlayved-btn" onclick="myFunction2()" style="padding-bottom: 15px;">Parts
 
     <button class="overlayved-btn" onclick="myFunction2()" style="padding-bottom: 15px;">Parts
 
       <i class="fa fa-caret-right"></i>   
 
       <i class="fa fa-caret-right"></i>   
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     <div class="dropdownsakks-container" id="mydropdownsakks2" style= "font-style: italic;">
 
     <div class="dropdownsakks-container" id="mydropdownsakks2" style= "font-style: italic;">
−
     <a href="#" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 3px;" >Basic Parts</a>
+
     <a href="https://2018.igem.org/Team:IIT_Delhi/Basic_Part" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 3px;" >Basic Parts</a>
−
     <a href="#" style="font-size: 15px;font-size: 15px; padding: 10px; padding-bottom: 5px; padding-top: 5px;" >Composite Parts</a>
+
     <a href="https://2018.igem.org/Team:IIT_Delhi/Composite_Part" style="font-size: 15px;font-size: 15px; padding: 10px; padding-bottom: 5px; padding-top: 5px;" >Composite Parts</a>
 
            
 
            
 
     </div>
 
     </div>
 +
 
 +
       
 +
    <button class="overlayved-btn" onclick="myFunction6()" style="padding-bottom: 15px;">Results
 +
      <i class="fa fa-caret-right"></i> 
 +
    </button>
 
      
 
      
−
         <a href="#safety" style="padding-bottom: 15px;padding-top: 15px;" >Modelling</a>
+
    <div class="dropdownsakks-container" id="mydropdownsakks6" style= "font-style: italic;">
 +
    <a href="https://2018.igem.org/Team:IIT_Delhi/Demonstrate" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 3px;" >Demonstrate</a>
 +
    <a href="https://2018.igem.org/Team:IIT_Delhi/InterLab" style="font-size: 15px;font-size: 15px; padding: 10px; padding-bottom: 5px; padding-top: 5px;" >Interlab</a>
 +
         
 +
    </div>
 +
   
 +
         <a href="https://2018.igem.org/Team:IIT_Delhi/Model" style="padding-bottom: 15px;padding-top: 15px;" >Modelling</a>
 
          
 
          
 
     <button class="overlayved-btn" onclick="myFunction3()" style="padding-bottom: 15px;" >Human Practices
 
     <button class="overlayved-btn" onclick="myFunction3()" style="padding-bottom: 15px;" >Human Practices
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     <div class="dropdownsakks-container" id="mydropdownsakks3" style= "font-style: italic;"  >
 
     <div class="dropdownsakks-container" id="mydropdownsakks3" style= "font-style: italic;"  >
−
     <a href="#" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 0px; " >Human Practices</a>
+
     <a href="https://2018.igem.org/Team:IIT_Delhi/Human_Practices" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 0px; " >Human Practices</a>
−
     <a href="#" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 5px;" >Integrated Practices</a>
+
     <a href="https://2018.igem.org/Team:IIT_Delhi/Safety" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 5px;" >Safety</a>
−
    <a href="#" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 5px;" >Safety</a>
+
     <a href="https://2018.igem.org/Team:IIT_Delhi/Public_Engagement" style="font-size: 15px;padding: 10px; padding-top: 5px;
−
     <a href="#" style="font-size: 15px;padding: 10px; padding-top: 5px;
+
 
                       padding-bottom: 0px;" >Public Engagement</a>
 
                       padding-bottom: 0px;" >Public Engagement</a>
 
     </div>
 
     </div>
 
      
 
      
−
     <a href="#safety" style="padding-bottom: 15px;padding-top: 15px;" >Collaboration</a>
+
     <a href="https://2018.igem.org/Team:IIT_Delhi/Collaborations" style="padding-bottom: 15px;padding-top: 15px;" >Collaboration</a>
 
      
 
      
 
     <button class="overlayved-btn" onclick="myFunction4()" style="padding-bottom: 15px;padding-top: 15px;"  >Team
 
     <button class="overlayved-btn" onclick="myFunction4()" style="padding-bottom: 15px;padding-top: 15px;"  >Team
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     <div class="dropdownsakks-container" id="mydropdownsakks4" style= "font-style: italic;">
 
     <div class="dropdownsakks-container" id="mydropdownsakks4" style= "font-style: italic;">
−
     <a href="#" style="font-size: 15px; font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 0px;padding-bottom: 5px;" >Members</a>
+
     <a href="https://2018.igem.org/Team:IIT_Delhi/Team" style="font-size: 15px; font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 0px;padding-bottom: 5px;" >Members</a>
−
     <a href="#" style="font-size: 15px;padding: 10px; padding-top: 5px;
+
     <a href="https://2018.igem.org/Team:IIT_Delhi/Attributions" style="font-size: 15px;padding: 10px; padding-top: 5px;
 
                       padding-bottom: 5px;" >Attributions</a>
 
                       padding-bottom: 5px;" >Attributions</a>
−
         <a href="#" style="font-size: 15px;padding: 10px; padding-top: 5px;
+
          
−
                      padding-bottom: 0px;" >Sponsors</a>
+
 
     </div>
 
     </div>
 
      
 
      
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     <a href="#" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 0px; " >Lab Records</a>
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     <a href="https://2018.igem.org/Team:IIT_Delhi/Notebook" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 0px; " >Lab Records</a>
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    <a href="#" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 5px;" >Protocol</a>
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             <a href="https://igem.org/2018_Judging_Form?team=IIT_Delhi" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 5px;" >Medal Criteria</a>
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<p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;color: black;margin: auto;width: 75%;text-align: center;">
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<br><br>With the advent of synthetic biology, a lot of synthetic circuits are designed every now and then. These circuits contain a lot of components working in harmony to regulate the gene expression. In any system, as the number of components which work together increases, the need for those components to be orthogonal also increases. The components, if interfere with the working of each other, may cause the system as a whole to fail. Such systems succumb to the noise and growth-related factors present in the biological environment. Recombinases in a biological system are adept at this job and hence, are a good basis for the formation of large-scale networks. Utilizing serine recombinases in such biological circuits provides a solution to these problems, as they have non-identical recognition sites and a particular recombinase works orthogonally to other recombinases.<br>
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<br>
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These allow us to build complex circuits with genetic modification in relatively uncomplicated configurations owing to the small size of attB and attP sites(~50bp). The attP and attB sites (and thus the integrase-att complexes) have imperfect two-fold symmetry. Hence they allow recombination in two different ways, with the sites aligned in ‘parallel’ or ‘antiparallel’ configuration. Thus, the ability to integrate/excise the DNA provides flexibility in the design of the constructs.<br><br>
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<img src="https://static.igem.org/mediawiki/2018/6/61/T--IIT_Delhi--recombinase_action.png" style="border:3px solid #000000" width="80%">
  
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We at iGEM IIT Delhi made use of serine based recombinases - Bxb1 and TP901 to develop 2 recombinase-based incoherent feed-forward loop constructs. These constructs show a strong capability for regulating and reducing the expression variability of genes being transcribed in addition to faster response time compared to transcriptional networks.
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These constructs can be utilized in the development of complex circuits.<br><br>
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    <span class="border" style="border-style: double">Safety Form</span>
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  <p>We at iGEM understand the necessity of working in a safe environment. A safe environment ensures a non-hesitant mind that designs more efficient biological devices.
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    <p style="font-size: 19px">iGEM IIT Delhi has always been following all biosafety rules of our institution and concerned biosafety laws of our country.<br>
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EHLSU (Environmental Health and Lab Safety Unit) is responsible for holding up all the safety guidelines in all the labs (including ours) at the institution. The guidelines can be found on the link -<br>
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        <a href="#">http://dbtbiosafety.nic.in/guideline/pdf/guidelines_94.pdf</a> <br>
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The safety guidelines for all the labs in our region (North India) are declared and monitored by EHLSU, whose rules can be found on the following link - <br>
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        <a href="#">http://dbtbiosafety.nic.in/guideline/pdf/guidelines_94.pdf</a><br>
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iGEM IIT Delhi did not conduct any experiment with human subjects (including non-invasive experiments, such as surveys), for the surveys conducted we have followed the rules of our institution.<br>
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            <div style="text-align: center;margin-bottom: -136px;"><h4 style="font-size: 50px;"><u class="fx" style="cursor: pointer;text-align: center">Address</u></h4><p style="text-align: center;margin-bottom: 16px">Undergraduate Laboratory<br>
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Department of Biotechnology and Biochemical Engineering, IIT Delhi</p>
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Latest revision as of 03:35, 18 October 2018

iGEM IIT Delhi

Notebook
Lab Records Medal Criteria
Team
Members Attributions
Collaboration
Human Practices
Human Practices Safety Public Engagement
Modelling
Parts
Basic Parts Composite Parts
Results
Demonstrate Interlab
Project
Overview Design



With the advent of synthetic biology, a lot of synthetic circuits are designed every now and then. These circuits contain a lot of components working in harmony to regulate the gene expression. In any system, as the number of components which work together increases, the need for those components to be orthogonal also increases. The components, if interfere with the working of each other, may cause the system as a whole to fail. Such systems succumb to the noise and growth-related factors present in the biological environment. Recombinases in a biological system are adept at this job and hence, are a good basis for the formation of large-scale networks. Utilizing serine recombinases in such biological circuits provides a solution to these problems, as they have non-identical recognition sites and a particular recombinase works orthogonally to other recombinases.

These allow us to build complex circuits with genetic modification in relatively uncomplicated configurations owing to the small size of attB and attP sites(~50bp). The attP and attB sites (and thus the integrase-att complexes) have imperfect two-fold symmetry. Hence they allow recombination in two different ways, with the sites aligned in ‘parallel’ or ‘antiparallel’ configuration. Thus, the ability to integrate/excise the DNA provides flexibility in the design of the constructs.



We at iGEM IIT Delhi made use of serine based recombinases - Bxb1 and TP901 to develop 2 recombinase-based incoherent feed-forward loop constructs. These constructs show a strong capability for regulating and reducing the expression variability of genes being transcribed in addition to faster response time compared to transcriptional networks. These constructs can be utilized in the development of complex circuits.