Difference between revisions of "Team:Ecuador/Description"

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<h1>Description</h1>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h3>What should this page contain?</h3>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<h3>Inspiration</h3>
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<p>See how other teams have described and presented their projects: </p>
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<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
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<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
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<h3>Advice on writing your Project Description</h3>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements.  
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<h3>References</h3>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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      PROJECT OVERVIEW
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        Biomaterials have the ability to interact with biological systems without being rejected. Through interdisciplinary research that involves cell biology, materials science, and bioengineering, biomaterials have been developed as biological substitutes to restore normal cellular function of organs and tissues. A new biomaterial will be developed based on the cross-linking of bacterial cellulose (BC) and elastin-like polypeptides (ELP), for biomedical applications. The cross-linking of both materials will be carried out with synthetic biology techniques using Escherichia coli as expression system. The crosslinking will be mediated by a carbohydrate binding domain protein module (CBD) specific for cellulose. CBDs are protein modules found in carbohydratases whose function is to bind carbohydrate to bring it closer to the catalytic site of the enzyme. For this process, three expression vectors will be designed. The first one will code for the enzymes necessary in the synthesis of bacterial cellulose, the second for the optimization of extracellular production and transport of bacterial cellulose and the third one will code for a fusion protein formed by the elastin-like polypeptide and the carbohydrate binding domain (CBD-ELP). A mathematical model for the interaction between cellulose and the CBD-ELP polypeptide will be determined based on the results from in-vitro experiments. The product generated by the modified bacteria will be characterized by biochemical methods and studied using structural elucidation methods, including spectrophotometry, nuclear magnetic resonance, mass spectrometry, and X-ray diffraction and its morphology will be elucidated using of atomic force microscopy. We expect that the new biomaterial combine the structural properties of bacterial cellulose with the functionalization capacity and thermo-sensitivity of elastin, for applications in food and tissue engineering, regenerative medicine, and selective release of drugs.
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Latest revision as of 14:55, 17 November 2018

iGEM ECUADOR
C-lastin, Interlab
PROJECT OVERVIEW
Biomaterials have the ability to interact with biological systems without being rejected. Through interdisciplinary research that involves cell biology, materials science, and bioengineering, biomaterials have been developed as biological substitutes to restore normal cellular function of organs and tissues. A new biomaterial will be developed based on the cross-linking of bacterial cellulose (BC) and elastin-like polypeptides (ELP), for biomedical applications. The cross-linking of both materials will be carried out with synthetic biology techniques using Escherichia coli as expression system. The crosslinking will be mediated by a carbohydrate binding domain protein module (CBD) specific for cellulose. CBDs are protein modules found in carbohydratases whose function is to bind carbohydrate to bring it closer to the catalytic site of the enzyme. For this process, three expression vectors will be designed. The first one will code for the enzymes necessary in the synthesis of bacterial cellulose, the second for the optimization of extracellular production and transport of bacterial cellulose and the third one will code for a fusion protein formed by the elastin-like polypeptide and the carbohydrate binding domain (CBD-ELP). A mathematical model for the interaction between cellulose and the CBD-ELP polypeptide will be determined based on the results from in-vitro experiments. The product generated by the modified bacteria will be characterized by biochemical methods and studied using structural elucidation methods, including spectrophotometry, nuclear magnetic resonance, mass spectrometry, and X-ray diffraction and its morphology will be elucidated using of atomic force microscopy. We expect that the new biomaterial combine the structural properties of bacterial cellulose with the functionalization capacity and thermo-sensitivity of elastin, for applications in food and tissue engineering, regenerative medicine, and selective release of drugs.
SPONSORS
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CONTACT US
biosin.espe@outlook.es

UNIVERSIDAD DE LAS FUERZAS ARMADAS ESPE.
Ecuador,Sangolquí, Av. General Rumiñahui s/n y Ambato