Difference between revisions of "Team:Ecuador/Parts"
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} | } | ||
.ec--p{ | .ec--p{ | ||
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<div class="ec--nau--content"> | <div class="ec--nau--content"> | ||
| − | <a name="INT"> | + | <a name="INT"></a> |
<div class="ec--h2"> | <div class="ec--h2"> | ||
INTRODUCTION | INTRODUCTION | ||
</div> | </div> | ||
| − | + | ||
<div class="ec--p"> | <div class="ec--p"> | ||
The aim of our project is to obtain a novel bone and cartilage regeneration treatment. The path we take to carry out this was transforming E. coli to produce a fusion protein, conformed by a cellulose binding domain and a protein that induce chondrogenesis and osteogenesis, also a fusion protein conformed by CBD and an elastin like protein was design in order to obtain an elastic biomaterial. We also tried to produce cellulose in E. coli to have a better control in its production with a LacI repressed promoter. The parts are briefly described below (see the part page if more information is needed). | The aim of our project is to obtain a novel bone and cartilage regeneration treatment. The path we take to carry out this was transforming E. coli to produce a fusion protein, conformed by a cellulose binding domain and a protein that induce chondrogenesis and osteogenesis, also a fusion protein conformed by CBD and an elastin like protein was design in order to obtain an elastic biomaterial. We also tried to produce cellulose in E. coli to have a better control in its production with a LacI repressed promoter. The parts are briefly described below (see the part page if more information is needed). | ||
</div> | </div> | ||
| − | <a name="BAC"> | + | <a name="BAC"></a> |
<div class="ec--h2"> | <div class="ec--h2"> | ||
Fusion protein for bone and cartilage regeneration | Fusion protein for bone and cartilage regeneration | ||
</div> | </div> | ||
| − | + | ||
<div class="ec--h3">Coding parts</div> | <div class="ec--h3">Coding parts</div> | ||
<div class="ec--BMP--container"> | <div class="ec--BMP--container"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2804000"><div class="ec--h4">BBa_K2804000 | + | <a href="http://parts.igem.org/Part:BBa_K2804000" style="color:black;"><div class="ec--h4" style="color:black;">BBa_K2804000 |
</div></a> | </div></a> | ||
| Line 226: | Line 227: | ||
<div class="ec--BMP--container"> | <div class="ec--BMP--container"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2804006"><div class="ec--h4"> | + | <a href="http://parts.igem.org/Part:BBa_K2804006" style="color:black;"><div class="ec--h4"> |
BBa_K2804006 | BBa_K2804006 | ||
| Line 238: | Line 239: | ||
<div class="ec--BMP--container"> | <div class="ec--BMP--container"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2804002"><div class="ec--h4">BBa_K2804002 | + | <a href="http://parts.igem.org/Part:BBa_K2804002" style="color:black;"><div class="ec--h4">BBa_K2804002 |
</div></a> | </div></a> | ||
<strong>Bone morphogenetic protein 2 (BMP2)</strong> | <strong>Bone morphogenetic protein 2 (BMP2)</strong> | ||
<div class="ec--BMP--text"> | <div class="ec--BMP--text"> | ||
<div class="ec--p"> | <div class="ec--p"> | ||
| − | Bone morphogenetic protein 2 (BMP2), is a member of the transforming growth factor-β (TGF-β) super-family. It is involved in chondrogenesis and osteogenesis in vitro via the expression induction of Sox9, Runx2 and its downstream markers.<sup>1 | + | Bone morphogenetic protein 2 (BMP2), is a member of the transforming growth factor-β (TGF-β) super-family. It is involved in chondrogenesis and osteogenesis in vitro via the expression induction of Sox9, Runx2 and its downstream markers.<sup>1</sup> |
</div> | </div> | ||
| Line 251: | Line 252: | ||
<div class="ec--BMP--container"> | <div class="ec--BMP--container"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2804001"><div class="ec--h4">BBa_K2804001 | + | <a href="http://parts.igem.org/Part:BBa_K2804001" style="color:black;"><div class="ec--h4">BBa_K2804001 |
</div></a> | </div></a> | ||
<strong>CBD cipA fused to BMP2 under the control of LacI promoter</strong> | <strong>CBD cipA fused to BMP2 under the control of LacI promoter</strong> | ||
| Line 259: | Line 260: | ||
</div> | </div> | ||
<div class="ec--BMP--container"> | <div class="ec--BMP--container"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2804004"><div class="ec--h4">BBa_K2804004 | + | <a href="http://parts.igem.org/Part:BBa_K2804004" style="color:black;"><div class="ec--h4">BBa_K2804004 |
</div></a> | </div></a> | ||
CBD cipA fused to sfGFP | CBD cipA fused to sfGFP | ||
| Line 268: | Line 269: | ||
<div class="ec--BMP--container"> | <div class="ec--BMP--container"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2804005"><div class="ec--h4">BBa_K2804005 | + | <a href="http://parts.igem.org/Part:BBa_K2804005" style="color:black;"><div class="ec--h4">BBa_K2804005 |
</div></a> | </div></a> | ||
CBD cipA fused to sfGFP under the control of LacI promoter | CBD cipA fused to sfGFP under the control of LacI promoter | ||
| Line 276: | Line 277: | ||
</div> | </div> | ||
<div class="ec--BMP--container"> | <div class="ec--BMP--container"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2804014"><div class="ec--h4">BBa_K2804014 | + | <a href="http://parts.igem.org/Part:BBa_K2804014" style="color:black;"><div class="ec--h4">BBa_K2804014 |
</div></a> | </div></a> | ||
CBD cipA fused to sfGFP | CBD cipA fused to sfGFP | ||
| Line 284: | Line 285: | ||
</div> | </div> | ||
<div class="ec--BMP--container"> | <div class="ec--BMP--container"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2804015"><div class="ec--h4">BBa_K2804015 | + | <a href="http://parts.igem.org/Part:BBa_K2804015" style="color:black;"><div class="ec--h4">BBa_K2804015 |
</div></a> | </div></a> | ||
CBD cipA fused to BMP2 | CBD cipA fused to BMP2 | ||
| Line 340: | Line 341: | ||
<p> </p> | <p> </p> | ||
| − | <a name="ELA"> | + | <a name="ELA"></a> |
<div class="ec--h2"> | <div class="ec--h2"> | ||
Fusion protein to provide elasticity to the biomaterial | Fusion protein to provide elasticity to the biomaterial | ||
</div> | </div> | ||
| − | + | ||
<div class="ec--h3">Coding parts</div> | <div class="ec--h3">Coding parts</div> | ||
<div class="ec--BMP--container"> | <div class="ec--BMP--container"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2804011"><div class="ec--h4">BBa_K2804011 | + | <a href="http://parts.igem.org/Part:BBa_K2804011" style="color:black;"><div class="ec--h4">BBa_K2804011 |
</div></a> | </div></a> | ||
ELP V-150 | ELP V-150 | ||
| Line 356: | Line 357: | ||
</div> | </div> | ||
<div class="ec--BMP--container"> | <div class="ec--BMP--container"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2804012"><div class="ec--h4">BBa_K2804012 | + | <a href="http://parts.igem.org/Part:BBa_K2804012" style="color:black;"><div class="ec--h4">BBa_K2804012 |
</div></a> | </div></a> | ||
ELP V-150 | ELP V-150 | ||
| Line 367: | Line 368: | ||
<div class="ec--BMP--container"> | <div class="ec--BMP--container"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2804013"><div class="ec--h4">BBa_K2804013 | + | <a href="http://parts.igem.org/Part:BBa_K2804013" style="color:black;"><div class="ec--h4">BBa_K2804013 |
</div></a> | </div></a> | ||
CBD cipA fused to ELP_C5 under the control of LacI promoter | CBD cipA fused to ELP_C5 under the control of LacI promoter | ||
| Line 398: | Line 399: | ||
<p> </p> | <p> </p> | ||
| − | <a name="CELL"> | + | <a name="CELL"></a> |
<div class="ec--h2"> | <div class="ec--h2"> | ||
Cellulose production module | Cellulose production module | ||
</div> | </div> | ||
| − | + | ||
<div class="ec--h3">Coding parts</div> | <div class="ec--h3">Coding parts</div> | ||
<div class="ec--BMP--container"> | <div class="ec--BMP--container"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2804008"> | + | <a href="http://parts.igem.org/Part:BBa_K2804008" style="color:black;"> |
<div class="ec--h4"> | <div class="ec--h4"> | ||
BBa_K2804008 | BBa_K2804008 | ||
| Line 417: | Line 418: | ||
</div> | </div> | ||
<div class="ec--BMP--container"> | <div class="ec--BMP--container"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2804009"><div class="ec--h4">BBa_K2804009 | + | <a href="http://parts.igem.org/Part:BBa_K2804009" style="color:black;"><div class="ec--h4">BBa_K2804009 |
</div></a> | </div></a> | ||
CcpAx: Cellulose-complementing protein | CcpAx: Cellulose-complementing protein | ||
| Line 429: | Line 430: | ||
<div class="ec--BMP--container"> | <div class="ec--BMP--container"> | ||
| − | <a href="http://parts.igem.org/Part:BBa_K2804010"><div class="ec--h4">BBa_K2804010 | + | <a href="http://parts.igem.org/Part:BBa_K2804010" style="color:black;"><div class="ec--h4">BBa_K2804010 |
</div></a> | </div></a> | ||
Operon member of bacterial cellulose synthase complex: Cmcax and CcpAx | Operon member of bacterial cellulose synthase complex: Cmcax and CcpAx | ||
| Line 460: | Line 461: | ||
</div> | </div> | ||
</body> | </body> | ||
| − | <div class="ec--h4"><a name="RE"> | + | <div class="ec--h4"><a name="RE"></a>REFERENCES</div> |
<div class="ec--p"> | <div class="ec--p"> | ||
<div class="ec--p">Zhou, N., Li, Q., Lin, X. et al. Cell Tissue Res (2016) 366: 101. <a href="https://doi.org/10.1007/s00441-016-2403-0">https://doi.org/10.1007/s00441-016-2403-0</a></div> | <div class="ec--p">Zhou, N., Li, Q., Lin, X. et al. Cell Tissue Res (2016) 366: 101. <a href="https://doi.org/10.1007/s00441-016-2403-0">https://doi.org/10.1007/s00441-016-2403-0</a></div> | ||
| − | </div> | + | </div> |
| + | </div> | ||
<script src="https://ajax.googleapis.com/ajax/libs/jquery/3.3.1/jquery.min.js"></script> | <script src="https://ajax.googleapis.com/ajax/libs/jquery/3.3.1/jquery.min.js"></script> | ||
<script> | <script> | ||
Latest revision as of 02:32, 19 November 2018
INTRODUCTION
The aim of our project is to obtain a novel bone and cartilage regeneration treatment. The path we take to carry out this was transforming E. coli to produce a fusion protein, conformed by a cellulose binding domain and a protein that induce chondrogenesis and osteogenesis, also a fusion protein conformed by CBD and an elastin like protein was design in order to obtain an elastic biomaterial. We also tried to produce cellulose in E. coli to have a better control in its production with a LacI repressed promoter. The parts are briefly described below (see the part page if more information is needed).
Fusion protein for bone and cartilage regeneration
Coding parts
BBa_K2804000
CBD cipA with a C-terminal linker
This part was design to try to improve the imperial college (2014) CBD cipA part BBa_K1321014, because the CBD N-terminal is where the protein binds to the cellulose and the linker could interfer with this interaction.
BBa_K2804006
Superfolder GFP with six-histidine tail
The adition of an histidine tail to part BBa_K1365020 was done because it allows the characterization of fusion proteins binding sites through atomic force microscopy, thanks to its affinity to the equipment nickel tip.
BBa_K2804002
Bone morphogenetic protein 2 (BMP2)
Bone morphogenetic protein 2 (BMP2), is a member of the transforming growth factor-β (TGF-β) super-family. It is involved in chondrogenesis and osteogenesis in vitro via the expression induction of Sox9, Runx2 and its downstream markers.1
Composite parts
BBa_K2804001
CBD cipA fused to BMP2 under the control of LacI promoter
Therapeutic protein coupled to the CBD whose expression is regulated by the presence of IPTG in the medium.
BBa_K2804004
CBD cipA fused to sfGFP
BBa_K2804005
CBD cipA fused to sfGFP under the control of LacI promoter
BBa_K2804014
CBD cipA fused to sfGFP
BBa_K2804015
CBD cipA fused to BMP2
Name |
Description |
Type |
BBa_K2804000 |
CBD cipA with a C-terminal linker |
Coding |
BBa_K2804001 |
CBD cipA fused to BMP2 under the control of LacI promoter |
Composite |
BBa_K2804002 |
Bone morphogenetic protein 2 (BMP2) |
Coding |
BBa_K2804004 |
CBD cipA fused to sfGFP |
Composite |
BBa_K2804005 |
CBD cipA fused to sfGFP under the control of LacI promoter |
Composite |
BBa_K2804006 |
Superfolder GFP with six-histidine tail |
Coding |
BBa_K2804014 |
CBD cipA fused to sfGFP |
Composite |
BBa_K2804015 |
CBD cipA fused to BMP2 |
Composite |
Fusion protein to provide elasticity to the biomaterial
Coding parts
BBa_K2804011
ELP V-150
BBa_K2804012
ELP V-150
Composite part
BBa_K2804013
CBD cipA fused to ELP_C5 under the control of LacI promoter
Name |
Description |
Type |
BBa_K2804011 |
ELP V-150 |
Coding |
BBa_K2804012 |
ELP_C5 |
Coding |
BBa_K2804013 |
CBD cipA fused to ELP_C5 under the control of LacI promoter |
Composite |
Cellulose production module
Coding parts
BBa_K2804008
CmcAx: Endo-beta-1,4-glucanase ezyme
BBa_K2804009
CcpAx: Cellulose-complementing protein
Composite part
BBa_K2804010
Operon member of bacterial cellulose synthase complex: Cmcax and CcpAx
Name |
Description |
Type |
BBa_K2804008 |
CmcAx: Endo-beta-1,4-glucanase ezyme |
Coding |
BBa_K2804009 |
CcpAx: Cellulose-complementing protein |
Coding |
BBa_K2804010 |
Operon member of bacterial cellulose synthase complex: Cmcax and CcpAx |
Composite |
Zhou, N., Li, Q., Lin, X. et al. Cell Tissue Res (2016) 366: 101. https://doi.org/10.1007/s00441-016-2403-0