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− | The final pathway we tested in the lab, consists of dehalogenase, dioxygenase and hydrolase enzymes. Additionally, Cyp 450 and Lignin Peroxidase enzymes were researched, enzymes have been | + | The final pathway we tested in the lab, consists of dehalogenase, dioxygenase and hydrolase enzymes. Additionally, Cyp 450 and Lignin Peroxidase enzymes were researched, enzymes have been previously found to breakdown TCDD. Despite that neither of these enzymes catalyze a specific step in the final engineered pathway, both are excellent candidates for TCDD Degradation. |
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Latest revision as of 17:29, 12 December 2018
Our team has created an exploratory model of a metabolic pathway for the enzymatic degradation of TCDD. This model served as the foundation of our project design and the backbone for our lab work. We completed in depth research on enzymes families relevant to TCDD degradation, narrowing down contenders to the enzymes we would eventually use in the lab. While building this model, we utilized a multitude of tools including, but not limited to biochemical analysis, phylogenetic comparison and pathway mapping. Through these tools, we compiled a comprehensive list of enzymes, is strongly rooted in prior academic studies.
The final pathway we tested in the lab, consists of dehalogenase, dioxygenase and hydrolase enzymes. Additionally, Cyp 450 and Lignin Peroxidase enzymes were researched, enzymes have been previously found to breakdown TCDD. Despite that neither of these enzymes catalyze a specific step in the final engineered pathway, both are excellent candidates for TCDD Degradation.