Difference between revisions of "Team:HebrewU/InterLab"

 
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+
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</style>
 
</style>
−
<body>
 
  
−
<ul id="ddmenu_hebrewu">
+
<script>
 +
google.charts.load('current', {'packages':['corechart', 'line']});
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    google.charts.setOnLoadCallback(drawChart1);
 +
google.charts.setOnLoadCallback(drawChart2);
 +
 
 +
<!--- Graph 1 --->
 +
 
 +
    function drawChart1() {
 +
 
 +
      var data = new google.visualization.DataTable();
 +
      data.addColumn('number', 'Pcounts');
 +
      data.addColumn('number', 'Abs');
 +
 
 +
      data.addRows([
 +
          [ 2.35E+08, 0.977],
 +
          [ 1.18E+08, 0.568],
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 +
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 +
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 +
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 +
 
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        vAxis: {
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 +
 
 +
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 +
        vAxis: {
 +
          title: 'Abs 600',
 +
          scaleType: 'log',
 +
          ticks: [0.01, 0.1, 1]
 +
        }
 +
 +
      };
 +
 
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 +
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 +
 
 +
      var logChart = new google.visualization.LineChart(document.getElementById('log_div'));
 +
      logChart.draw(data, logOptions);
 +
    }
 
 
−
     <div class="huji_homebutton" >
+
<!--- Graph 2 information--->
−
    <li><a href="#">HebrewU</a></li>
+
 
−
    </div>  
+
function drawChart2() {
−
   
+
 
−
     <li><a href="#">Project</a>
+
      var data = new google.visualization.DataTable();
 +
      data.addColumn('number', 'Pcounts');
 +
      data.addColumn('number', 'Abs');
 +
 
 +
      data.addRows([
 +
          [10.00, 6.461E+06],
 +
          [5.00, 3.551E+06],
 +
          [2.5, 1.900E+06],
 +
          [1.25, 9.253E+05],
 +
          [0.625, 4.844E+05],
 +
          [0.313, 2.420E+05],
 +
          [0.156, 1.162E+05],
 +
          [0.078, 5.755E+04],
 +
          [0.039, 3.016E+04],
 +
          [0.0195,1.527E+04],
 +
          [0.0098,7.731E+03],
 +
      ]);
 +
 
 +
 +
 +
 +
 +
      var linearOptions2 = {
 +
        title: 'Fluorescein Standard Curve (Linear Scale), R² = 0.997',
 +
        legend: 'none',
 +
        width: 350,
 +
        height: 300,
 +
pointSize: 12,
 +
        dataOpacity: 0.3,
 +
        colors: ['black'],
 +
        backgroundColor: '#edeadc',
 +
        hAxis: {
 +
          title: 'Fluorescein Concentration (uM)',
 +
  ticks: [0,2,4,6,8,10]
 +
 
 +
        },
 +
        vAxis: {
 +
          title: 'Fluorescence',
 +
 
 +
        }
 +
 +
      };
 +
 
 +
      var logOptions2 = {
 +
        title: 'Fluorescein Standard Curve (Log Scale)',
 +
        legend: 'none',
 +
        width: 350,
 +
        height: 300,
 +
pointSize: 12,
 +
        dataOpacity: 0.3,
 +
        colors: ['black'],
 +
        backgroundColor: '#edeadc',
 +
        hAxis: {
 +
        title: 'Fluorescein Concentration (uM)',
 +
ticks: [0.01, 0.1, 1, 10]
 +
 
 +
 
 +
        },
 +
        vAxis: {
 +
          title: 'Fluorescence',
 +
          scaleType: 'log',
 +
  format: 'scientific',
 +
  ticks: [1E5, 2E6, 3E7, 4E8, 5E8, 6E8, 7E8, 8E8]
 +
 
 +
        }
 +
 +
      };
 +
 
 +
      var linearChart2 = new google.visualization.LineChart(document.getElementById('linear_div2'));
 +
      linearChart2.draw(data, linearOptions2);
 +
 
 +
      var logChart2 = new google.visualization.LineChart(document.getElementById('log_div2'));
 +
      logChart2.draw(data, logOptions2);
 +
     }
 +
 +
 +
 +
</script>
 +
 
 +
 
 +
<script>
 +
function myFunction() {
 +
    var x = document.getElementById("Industry_display");
 +
    if (x.className.indexOf("w3-show") == -1) {
 +
        x.className += " w3-show";
 +
    } else {
 +
        x.className = x.className.replace(" w3-show", "");
 +
    }
 +
}
 +
</script>
 +
 
 +
<script>
 +
function myFunction_2() {
 +
    var x = document.getElementById("Interviews_display");
 +
    if (x.className.indexOf("w3-show") == -1) {
 +
        x.className += " w3-show";
 +
    } else {
 +
        x.className = x.className.replace(" w3-show", "");
 +
    }
 +
}
 +
</script>
 +
 
 +
<body>
 +
<div class="w3-blue-gray">
 +
<ul id="ddmenu_hebrewu">
 +
            <li style="float:left;margin-top:12px;padding-left:15px;"><br />Hebrew</li>
 +
 
 +
    <div class="huji_homebutton">
 +
           
 +
        <a href="javascript:void(0);" class="icon" onClick="show_small_nav()">
 +
            <img src="https://static.igem.org/mediawiki/2018/d/dc/T--Hebrewu--huji_small_manu.png" width="20" height="20" padding:"2px">
 +
        </a>
 +
   
 +
        <a href="https://2018.igem.org/Team:HebrewU">
 +
                <img class="ulogo" src="https://static.igem.org/mediawiki/2018/d/d3/T--Hebrewu--huji_boiling_logo.png" width="60" height="60" style="margin-left:-27px;">
 +
                </a>
 +
  </div>  
 +
 
 +
     <li><a>Project</a>
 
         <ul>
 
         <ul>
−
             <li><a href="#">Motivation</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Description">Description</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/description">Description</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Model">Model</a></li>
−
             <li><a href="#">Results</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Demonstrate">Results</a></li>
−
             <li><a href="#">BioBricks</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Parts">Parts</a></li>
−
         </ul>
+
            <li><a href="https://2018.igem.org/Team:HebrewU/Software">MOOLTi</a></li>
−
               
+
 
 +
         </ul>      
 
     </li>
 
     </li>
−
    <li><a href="#">Team</a>
+
    <li><a>Lab</a>
 
         <ul>
 
         <ul>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Team">Members</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Design">Yeast Design</a></li>
−
             <li><a href="#">Attribiutions</a></li>
+
            <li><a href="https://2018.igem.org/Team:HebrewU/Plant">Plant Design</a></li>
−
             <li><a href="#">Sponsors</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Notebook">Notebook</a></li>
−
             <li><a href="#">Photo Gallery</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/InterLab">Inter-lab</a></li>
 +
             <li><a href="https://2018.igem.org/Team:HebrewU/Safety">Safety</a></li>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Protocols">Protocols</a></li>
 
         </ul>
 
         </ul>
 +
       
 
     </li>
 
     </li>
 +
 
 
      
 
      
−
     <li><a href="#">Human Practice</a>
+
     <li><a href="https://2018.igem.org/Team:HebrewU/Human_Practices">Human Practices</a>
 
         <ul>
 
         <ul>
−
             <li><a href="#">Citizen Survey</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Ethics">Ethics</a></li>
−
             <li><a href="#">Expert Interviews</a></li>
+
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<!--- Page content start --->
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Universal and standardized units are important in all disciplines of science. It allows students and professionals everywhere to understand the same language when describing substances or functions. However, the fields of biology and synthetic biology in particular often lack standardized units. For example, in genetic studies or modifications, we often employ marker proteins to assist in both real-time assessments and result interpretation. For results to be consistent between experiments and labs, it is of paramount importance to create a standardized unit for said markers. Such a unit would also allow for the precise comparison of results from different labs and machines, instead of relying on visual comparisons.  <br>
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    <br>
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  <h2 class="ahuji">Inter-Lab</h2>
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    The InterLab Study utilizes iGEM’s broad network of biology students from around the world to improve the measurement tools available to the iGEM community and the synthetic biology community as a whole. By measuring GFP fluorescence on a wide range of machines in many different labs and relating the results to a single control group, we hope to gather enough data to create a standardized unit for it. Since GFP is one of the most frequently used markers today, this has been a major focus of the InterLab studies over the past 5 years.
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    Our team understands the need for unit standardization within the world of biology. We want to help make progress towards more precise quantitative measurements in biology research. As such, we are excited to take part in the Interlab Study and hope our data will support the progress of synthetic biology and genetic engineering.
  
−
General Discription - what is Interlab (general purpose, our specific experiment). 
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 +
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    <th>N. of Particles</th>
 +
    <td>2.35E8</td>
 +
    <td>1.18E8</td>
 +
    <td>5.88E7</td>
 +
    <td>2.94E7</td>
 +
    <td>1.47E7</td>
 +
    <td>7.35E6</td>
 +
    <td>3.68E6</td>
 +
    <td>1.84E6</td>
 +
    <td>9.19E5</td>
 +
    <td>4.60E5</td>
 +
    <td>2.30E5</td>
  
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<br><br>
 
  
−
<b> We hope this research will help detoxify our planet, creating a safer and cleaner environment for everyone. <b>  
+
  </tr>
−
<br><br><br>
+
  <tr>
 +
    <th>Abs mean</th>
 +
    <td>0.977</td>
 +
    <td>0.568</td>
 +
    <td>0.305</td>
 +
    <td>0.195</td>
 +
    <td>0.139</td>
 +
    <td>0.113</td>
 +
    <td>0.098</td>
 +
    <td>0.092</td>
 +
    <td>0.087</td>
 +
    <td>0.086</td>
 +
    <td>0.086</td>
  
  
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 +
 
 +
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<button onClick="myFunction2()" class="hujigem_showdata_button">Raw data 2</button>
 +
 
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<table id="tablevalues_hujigem">
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    <th>Fluorescein (uM)</th>
 +
    <td>10.00</td>
 +
    <td>5.00</td>
 +
    <td>2.5</td>
 +
    <td>1.25</td>
 +
    <td>0.625</td>
 +
    <td>0.313</td>
 +
    <td>0.156</td>
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    <td>0.078</td>
 +
    <td>0.039</td>
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    <td>0.0195</td>
 +
    <td>0.0098</td>
 +
 
 +
 
 +
  </tr>
 +
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 +
    <th>Abs mean</th>
 +
    <td>6.461E6</td>
 +
    <td>3.551E6</td>
 +
    <td>1.900E6</td>
 +
    <td>9.253E5</td>
 +
    <td>4.844E5</td>
 +
    <td>2.420E5</td>
 +
    <td>1.162E5</td>
 +
    <td>5.755E4</td>
 +
    <td>3.016E4</td>
 +
    <td>1.527E4</td>
 +
    <td>7.731E3</td>
 +
 
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Latest revision as of 17:31, 12 December 2018

HebrewU HujiGEM 2018



Universal and standardized units are important in all disciplines of science. It allows students and professionals everywhere to understand the same language when describing substances or functions. However, the fields of biology and synthetic biology in particular often lack standardized units. For example, in genetic studies or modifications, we often employ marker proteins to assist in both real-time assessments and result interpretation. For results to be consistent between experiments and labs, it is of paramount importance to create a standardized unit for said markers. Such a unit would also allow for the precise comparison of results from different labs and machines, instead of relying on visual comparisons.  

The InterLab Study utilizes iGEM’s broad network of biology students from around the world to improve the measurement tools available to the iGEM community and the synthetic biology community as a whole. By measuring GFP fluorescence on a wide range of machines in many different labs and relating the results to a single control group, we hope to gather enough data to create a standardized unit for it. Since GFP is one of the most frequently used markers today, this has been a major focus of the InterLab studies over the past 5 years.  

Our team understands the need for unit standardization within the world of biology. We want to help make progress towards more precise quantitative measurements in biology research. As such, we are excited to take part in the Interlab Study and hope our data will support the progress of synthetic biology and genetic engineering.  




N. of Particles 2.35E8 1.18E8 5.88E7 2.94E7 1.47E7 7.35E6 3.68E6 1.84E6 9.19E5 4.60E5 2.30E5
Abs mean 0.977 0.568 0.305 0.195 0.139 0.113 0.098 0.092 0.087 0.086 0.086



Fluorescein (uM) 10.00 5.00 2.5 1.25 0.625 0.313 0.156 0.078 0.039 0.0195 0.0098
Abs mean 6.461E6 3.551E6 1.900E6 9.253E5 4.844E5 2.420E5 1.162E5 5.755E4 3.016E4 1.527E4 7.731E3