Difference between revisions of "Team:HebrewU/InterLab"

 
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<!--- THIS IS WHERE THE HTML BEGINS --->
 
 
<html lang="en">
 
<html lang="en">
 
<head>
 
<head>
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<meta charset="utf-8">
 
<meta charset="utf-8">
 
<meta name="viewport" content="width=device-width, initial-scale=1">
 
<meta name="viewport" content="width=device-width, initial-scale=1">
−
<link rel="stylesheet" href="https://2018.igem.org/Template:HebrewU/CSS?action=raw&ctype=text/css"
 
  
 +
<!--- Own CSS --->
 +
<link rel="stylesheet" href="https://2018.igem.org/Template:HebrewU/CSS?action=raw&ctype=text/css">
  
 +
<!--- Main Menu script --->
 +
<script type="text/javascript" src="https://2018.igem.org/Template:Hebrewu/Javascript?action=raw&ctype=text/javascript"></script>
 +
 +
<!--- w3 Css --->
 +
<link rel="stylesheet" href="https://2018.igem.org/Template:HebrewU/w3?action=raw&ctype=text/css">
 +
 +
<!--- Bootstrap Css --->
 +
  <script src="https://2018.igem.org/Template:HebrewU/boot?action=raw&ctype=text/javascript"></script>
 +
  <link rel="stylesheet" href="https://2018.igem.org/Template:HebrewU/strap?action=raw&ctype=text/css">
 +
 
 +
<style>
 +
/* Disable I-GEM defult properties for blank page */
 +
    #sideMenu, #top_title {display:none;}
 +
    #sideMenu, #sideMenu *, #top_menu_inside, #top_menu_inside *{box-sizing: initial; -webkit-box-sizing: initial;}
 +
    #content { padding:0px; width:100%; margin-top:-12px; margin-left:0px;}
 +
    #body {background-color:#EDEADC; }
 +
    #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
 +
 +
    .w3-container {
 +
  overflow: auto;
 +
    }
 +
 +
* {
 +
  -webkit-box-sizing:content-box ;
 +
    -moz-box-sizing:content-box ;
 +
    box-sizing:content-box ;
 +
    }
 +
 +
 +
.ahuji {
 +
    color:black
 +
    }
 +
 +
 +
 +
@media (max-width: 1000px) {
 +
    .fixed {
 +
    display: none;
 +
    }
 +
 +
.ulogo { display:none; }
 +
}
 +
 +
#panel, #flip_experts #panel_industry {
 +
    padding: 5px;
 +
    text-align: center;
 +
}
 +
 +
#panel panel_industry{
 +
    padding: 50px;
 +
    display: none;
 +
}
 +
 +
body {
 +
background-color:w3-blue-gray;
 +
 +
}
 +
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ul {
 +
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padding-left:150px;
 +
padding-right:100px;
 +
 +
 +
}
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a {
 +
    color: hotpink;
 +
}
 +
 +
#HQ_page p {
 +
    font-family: Tahoma, Geneva, sans-serif;
 +
    font-size:20px;
 +
    color: white;
 +
}
 +
 +
a:visited {
 +
    color: white;
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}
 +
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buttonhuji {
 +
font: 18px Calibri, Calibri;
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    border: none;
 +
    color: white;
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 +
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 +
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 +
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 +
    margin: 2px 2px;
 +
    -webkit-transition-duration: 0.4s; /* Safari */
 +
    transition-duration: 0.4s;
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}
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.buttonhuji1 {
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}
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.buttonhuji1:hover {
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    background-color: #829c8c;
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    color: white;
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}
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.hujigem_showdata_button {
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  background-color:#829c8c;
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  color: white;
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  margin: 4px 2px;
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  opacity: 0.6;
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  transition: 0.3s;
 +
  text-decoration: none;
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  cursor: pointer;
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  text-align:center
 +
 
 +
 
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}
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.hujigem_showdata_button:hover {opacity: 0.9}
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#hujigem_display_data{
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    width: 100%;
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    padding: 50px 0;
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    text-align: center;
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}
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#hujigem_showdata1,#hujigem_showdata2 {
 +
display:none;
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}
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table_huji {
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text-align: center;
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font-size-adjust:inherit;
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font-family:Calibri
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th_huji, td_huji {
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border: 1px solid #829c8c;
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font-family:Calibri;
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}
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body {background-color: #EDEADC;}
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#tablevalues_hujigem {
 +
background-color:white;
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font-family:Calibri;
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    text-align: center;
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border: 1px solid #829c8c;
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}
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</style>
  
−
 
−
<!--- Chart script --->
 
−
<script type="text/javascript" src="https://www.gstatic.com/charts/loader.js"></script>
 
 
<script>
 
<script>
 
google.charts.load('current', {'packages':['corechart', 'line']});
 
google.charts.load('current', {'packages':['corechart', 'line']});
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       var linearOptions = {
 
       var linearOptions = {
−
         title: 'Particle Standard Curve (Linear Scale)',
+
         title: 'Particle Standard Curve (Linear Scale), R^2 = 0.9986',
 
         legend: 'none',
 
         legend: 'none',
−
      showR2: true,
 
−
 
 
         width: 350,
 
         width: 350,
 
         height: 300,
 
         height: 300,
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           title: 'Particle Count / 100 uL',
 
           title: 'Particle Count / 100 uL',
 
  format: 'scientific'
 
  format: 'scientific'
 +
 
  
 
         },
 
         },
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           [10.00, 6.461E+06],
 
           [10.00, 6.461E+06],
 
           [5.00, 3.551E+06],
 
           [5.00, 3.551E+06],
−
           [ 2.5, 1.900E+06],
+
           [2.5, 1.900E+06],
−
           [ 1.25, 9.253E+05],
+
           [1.25, 9.253E+05],
−
           [ 0.625, 4.844E+05],
+
           [0.625, 4.844E+05],
−
           [ 0.313, 2.420E+05],
+
           [0.313, 2.420E+05],
−
           [ 0.156, 1.162E+05],
+
           [0.156, 1.162E+05],
−
           [ 0.078, 5.755E+04],
+
           [0.078, 5.755E+04],
−
           [ 0.039, 3.016E+04],
+
           [0.039, 3.016E+04],
−
           [ 0.0195,1.527E+04],
+
           [0.0195,1.527E+04],
−
           [ 0.0098,7.731E+03],
+
           [0.0098,7.731E+03],
 
       ]);
 
       ]);
  
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       var linearOptions2 = {
 
       var linearOptions2 = {
−
         title: 'Particle Standard Curve (Linear Scale)',
+
         title: 'Fluorescein Standard Curve (Linear Scale), R² = 0.997',
 
         legend: 'none',
 
         legend: 'none',
 
         width: 350,
 
         width: 350,
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         vAxis: {
 
         vAxis: {
 
           title: 'Fluorescence',
 
           title: 'Fluorescence',
−
  ticks: [1E5, 2E6, 3E7, 4E8, 5E8, 6E8, 7E8, 8E8]
 
  
 
         }
 
         }
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       var logOptions2 = {
 
       var logOptions2 = {
−
         title: 'Particle Standard Curve (Log Scale)',
+
         title: 'Fluorescein Standard Curve (Log Scale)',
 
         legend: 'none',
 
         legend: 'none',
 
         width: 350,
 
         width: 350,
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         hAxis: {
 
         hAxis: {
 
         title: 'Fluorescein Concentration (uM)',
 
         title: 'Fluorescein Concentration (uM)',
−
ticks: [0.00, 0.01, 0.1, 1, 10]
+
ticks: [0.01, 0.1, 1, 10]
  
  
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</script>
 
</script>
  
−
<head>
 
−
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
 
−
<title>Untitled Document</title>
 
−
</head>
 
  
−
+
<script>
−
 
+
function myFunction() {
−
<script>  
+
     var x = document.getElementById("Industry_display");
−
     var timeout    = 500;
+
     if (x.className.indexOf("w3-show") == -1) {
−
     var closetimer = 0;
+
        x.className += " w3-show";
−
    var ddmenuitem = 0;
+
     } else {
−
       
+
         x.className = x.className.replace(" w3-show", "");
−
     function ddmenu_open(){
+
−
         ddmenu_canceltimer();
+
−
          ddmenu_close();
+
−
          ddmenuitem = $(this).find('ul').css('visibility', 'visible');
+
 
     }
 
     }
−
       
+
}
−
    function ddmenu_close(){
+
−
        if(ddmenuitem) ddmenuitem.css('visibility', 'hidden');
+
−
    }
+
−
       
+
−
    function ddmenu_timer(){
+
−
        closetimer = window.setTimeout(ddmenu_close, timeout);
+
−
    }
+
−
       
+
−
    function ddmenu_canceltimer(){ 
+
−
        if(closetimer){ 
+
−
            window.clearTimeout(closetimer);
+
−
                closetimer = null;
+
−
    }}
+
−
       
+
−
    $(document).ready(function(){ 
+
−
        $('#ddmenu_hebrewu > li').bind('mouseover', ddmenu_open)
+
−
        $('#ddmenu_hebrewu > li').bind('mouseout',  ddmenu_timer)
+
−
    });
+
−
       
+
−
    document.onclick = ddmenu_close;
+
 
</script>
 
</script>
  
−
 
+
<script>
−
<style>
+
function myFunction_2() {
−
 
+
     var x = document.getElementById("Interviews_display");
−
            #sideMenu, #top_title {display:none;}
+
     if (x.className.indexOf("w3-show") == -1) {
−
            #sideMenu, #sideMenu *, #top_menu_inside, #top_menu_inside *{box-sizing: initial; -webkit-box-sizing: initial;}
+
        x.className += " w3-show";
−
            #content { padding:0px; width:100%; margin-top:-7px; margin-left:0px;}
+
     } else {
−
            body {background-color:white; }
+
        x.className = x.className.replace(" w3-show", "");
−
            #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
+
−
 
+
−
#ddmenu_hebrewu {
+
−
     background:#262d2e;
+
−
     margin: 0;
+
−
    padding: 0;
+
−
    height:35px;
+
−
    font: 15px Calibri, Calibri;}
+
−
   
+
−
#ddmenu_hebrewu li {   
+
−
    float: left;
+
−
    list-style: none;
+
−
    font: 20px Calibri, Calibri;}
+
−
+
−
#ddmenu_hebrewu li a { 
+
−
    background:#262d2e;
+
−
    display: block;
+
−
    padding: 5px 20px;
+
−
    text-decoration: none;
+
−
    color: white;
+
−
}
+
−
+
−
 
+
−
+
−
#ddmenu_hebrewu li a:hover {  
+
−
    background: #2c3635;
+
−
}
+
−
   
+
−
#ddmenu_hebrewu li ul {
+
−
    margin: 3px 0 0 10px;
+
−
    padding: 0;
+
−
    position: absolute;
+
−
    visibility: hidden;
+
−
}
+
−
+
−
#ddmenu_hebrewu li ul li { 
+
−
    display:inline;
+
−
}
+
−
+
−
#ddmenu_hebrewu li ul li a {
+
−
    width: auto;   
+
−
    background: none;
+
−
    display: inline;
+
−
    color: black;
+
−
    font: 20px Calibri, Calibri;
+
−
    padding: 2px 10px;
+
−
}
+
−
+
−
#ddmenu_hebrewu li ul li a:hover { 
+
−
    background: none;
+
−
    color: #106375;
+
−
    padding: 2px 10px;
+
−
}
+
−
 
+
−
/* Homebutton 10% space */
+
−
.huji_homebutton {
+
−
    float: left;
+
−
     width: 13%;
+
−
 
+
−
}
+
−
 
+
−
/* Create three unequal columns that floats next to each other */
+
−
.columnhuji {
+
−
    float: left;
+
−
    padding: 10px;
+
−
}
+
−
 
+
−
/* Left and right column */
+
−
.columnhuji.side {
+
−
    width: 12%;
+
−
 
+
−
}
+
−
 
+
−
/* Middle column */
+
−
.columnhuji.middle {
+
−
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+
−
    background-color:#edeadc;
+
−
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+
−
   
+
−
   
+
−
   
+
−
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+
−
 
+
−
/* Clear floats after the columns */
+
−
.rowhuji:after {
+
−
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+
−
    display: table;
+
−
    clear: both;
+
−
}
+
−
 
+
−
/* Responsive layout - makes the three columns stack on top of each other instead of next to each other */
+
−
@media screen and (max-width: 600px) {
+
−
    .columnhuji.side, .columnhuji.middle {
+
−
        width: 100%;
+
 
     }
 
     }
 
}
 
}
 +
</script>
  
−
h2.ahuji {
 
−
    font: 50px Calibri, Calibri;
 
−
    text-align: center;
 
−
}
 
−
 
−
h2.chuji {
 
−
    font: 20px Calibri, Calibri;
 
−
    text-align: center;
 
−
}
 
−
 
−
p.ahuji {
 
−
    font: 18px Calibri, Calibri;
 
−
margin-left:5%;
 
−
margin-right:5%;
 
−
text-align:justify;
 
−
}
 
−
 
−
p.bhuji {
 
−
    font: 18px Calibri, Calibri;
 
−
text-align:center;
 
−
}
 
−
 
−
 
−
.buttonhuji {
 
−
font: 18px Calibri, Calibri;
 
−
    border: none;
 
−
    color: white;
 
−
    padding: 10px 32px;
 
−
    text-align: center;
 
−
    display: inline-block;
 
−
    font-size: 15px;
 
−
    margin: 2px 2px;
 
−
    -webkit-transition-duration: 0.4s; /* Safari */
 
−
    transition-duration: 0.4s;
 
−
    cursor: pointer;
 
−
}
 
−
 
−
.buttonhuji1 {
 
−
    background-color: #fdfaf2;
 
−
    color: black;
 
−
    border: 2px solid #829c8c;
 
−
}
 
−
 
−
.buttonhuji1:hover {
 
−
    background-color: #829c8c;
 
−
    color: white;
 
−
}
 
−
 
−
.centerhujiimg {
 
−
    margin: auto;
 
−
    width: 10%;
 
−
text-align: center;
 
−
}
 
−
 
−
 
−
.hujigem_showdata_button {
 
−
  background-color:#829c8c;
 
−
  border: none;
 
−
  color: white;
 
−
  padding: 10px 10px;
 
−
  text-align: center;
 
−
  font-size: 16px;
 
−
  margin: 4px 2px;
 
−
  opacity: 0.6;
 
−
  transition: 0.3s;
 
−
  text-decoration: none;
 
−
  cursor: pointer;
 
−
 
 
−
 
 
−
}
 
−
 
−
.hujigem_showdata_button:hover {opacity: 1}
 
−
 
−
 
−
#hujigem_display_data{
 
−
    width: 100%;
 
−
    padding: 50px 0;
 
−
    text-align: center;
 
−
    background-color: lightblue;
 
−
    margin-top: 20px;
 
−
 
−
}
 
−
 
−
#hujigem_showdata1,#hujigem_showdata2 {
 
−
display:none;
 
−
}
 
−
 
−
table_huji {
 
−
    border-collapse: collapse;
 
−
text-align: center;
 
−
font-size-adjust:inherit;
 
−
font-family:Calibri
 
−
 
−
}
 
−
 
−
th_huji, td_huji {
 
−
    text-align: center;
 
−
border: 1px solid #829c8c;
 
−
    padding: 2px;
 
−
    font-size-adjust:inherit;
 
−
font-family:Calibri;
 
−
 
−
}
 
−
 
−
#tablevalues_hujigem {
 
−
background-color:white;
 
−
font-family:Calibri;
 
−
    text-align: center;
 
−
border: 1px solid #829c8c;
 
−
 
−
 
−
 
−
}
 
−
 
−
 
−
</style>
 
 
<body>
 
<body>
 +
<div class="w3-blue-gray">
 +
<ul id="ddmenu_hebrewu">
 +
            <li style="float:left;margin-top:12px;padding-left:15px;"><br />Hebrew</li>
  
−
<ul id="ddmenu_hebrewu">
+
    <div class="huji_homebutton">
−
+
           
−
    <div class="huji_homebutton" >
+
        <a href="javascript:void(0);" class="icon" onClick="show_small_nav()">
−
    <li><a href="#">HebrewU</a></li>
+
            <img src="https://static.igem.org/mediawiki/2018/d/dc/T--Hebrewu--huji_small_manu.png" width="20" height="20" padding:"2px">
−
    </div>  
+
        </a>
−
   
+
   
−
     <li><a href="#">Project</a>
+
        <a href="https://2018.igem.org/Team:HebrewU">
 +
                <img class="ulogo" src="https://static.igem.org/mediawiki/2018/d/d3/T--Hebrewu--huji_boiling_logo.png" width="60" height="60" style="margin-left:-27px;">
 +
                </a>
 +
  </div>  
 +
 
 +
     <li><a>Project</a>
 
         <ul>
 
         <ul>
−
             <li><a href="#">Motivation</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Description">Description</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/description">Description</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Model">Model</a></li>
−
             <li><a href="#">Results</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Demonstrate">Results</a></li>
−
             <li><a href="#">BioBricks</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Parts">Parts</a></li>
−
         </ul>
+
            <li><a href="https://2018.igem.org/Team:HebrewU/Software">MOOLTi</a></li>
−
               
+
 
 +
         </ul>      
 
     </li>
 
     </li>
−
    <li><a href="#">Team</a>
+
    <li><a>Lab</a>
 
         <ul>
 
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             <li><a href="https://2018.igem.org/Team:HebrewU/Team">Members</a></li>
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Universal and Standardized units are important in all disciplines of science, and especially in engineering. One important issue biology, and specifically genetic engineering faces today is a lack of standardized units. In synthetic biology we often employ marker proteins to assist in both real time assessment and in depth result interpretation. For these results to be consistent both between experiments, and between labs it is of paramount importance to create a standardized unit for said markers. Such a unit would also allow for the comparison of results from different labs and machines.
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The InterLab Study utilizes the wide access that iGEM has to biology teams all over the world. Taking advantage of this rare opportunity, the InterLab aims to improve the measurement tools available to both the iGEM community and the synthetic biology community as a whole. By measuring the fluorescence on a wide range of machines, in a wide range of labs, and regulating the results to a single control group, we hope to gather enough data to create this standard unit. As GFP is one of the most used markers today, this has been the focus of the InterLab studies over the past 5 years.
 
  
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Our team too believes in the need for a standardization of units with in the world of biology. We want to help make progress towards quantitative measurements between labs, and not be constrained to relative compassions. As such, were excited to take part in such a project and hope are data will support the progress of synthetic biology and genetic engineering.  
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Universal and standardized units are important in all disciplines of science. It allows students and professionals everywhere to understand the same language when describing substances or functions. However, the fields of biology and synthetic biology in particular often lack standardized units. For example, in genetic studies or modifications, we often employ marker proteins to assist in both real-time assessments and result interpretation. For results to be consistent between experiments and labs, it is of paramount importance to create a standardized unit for said markers. Such a unit would also allow for the precise comparison of results from different labs and machines, instead of relying on visual comparisons.  <br>
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     The InterLab Study utilizes iGEM’s broad network of biology students from around the world to improve the measurement tools available to the iGEM community and the synthetic biology community as a whole. By measuring GFP fluorescence on a wide range of machines in many different labs and relating the results to a single control group, we hope to gather enough data to create a standardized unit for it. Since GFP is one of the most frequently used markers today, this has been a major focus of the InterLab studies over the past 5 years.
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    Our team understands the need for unit standardization within the world of biology. We want to help make progress towards more precise quantitative measurements in biology research. As such, we are excited to take part in the Interlab Study and hope our data will support the progress of synthetic biology and genetic engineering.
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Latest revision as of 17:31, 12 December 2018

HebrewU HujiGEM 2018



Universal and standardized units are important in all disciplines of science. It allows students and professionals everywhere to understand the same language when describing substances or functions. However, the fields of biology and synthetic biology in particular often lack standardized units. For example, in genetic studies or modifications, we often employ marker proteins to assist in both real-time assessments and result interpretation. For results to be consistent between experiments and labs, it is of paramount importance to create a standardized unit for said markers. Such a unit would also allow for the precise comparison of results from different labs and machines, instead of relying on visual comparisons.  

The InterLab Study utilizes iGEM’s broad network of biology students from around the world to improve the measurement tools available to the iGEM community and the synthetic biology community as a whole. By measuring GFP fluorescence on a wide range of machines in many different labs and relating the results to a single control group, we hope to gather enough data to create a standardized unit for it. Since GFP is one of the most frequently used markers today, this has been a major focus of the InterLab studies over the past 5 years.  

Our team understands the need for unit standardization within the world of biology. We want to help make progress towards more precise quantitative measurements in biology research. As such, we are excited to take part in the Interlab Study and hope our data will support the progress of synthetic biology and genetic engineering.  




N. of Particles 2.35E8 1.18E8 5.88E7 2.94E7 1.47E7 7.35E6 3.68E6 1.84E6 9.19E5 4.60E5 2.30E5
Abs mean 0.977 0.568 0.305 0.195 0.139 0.113 0.098 0.092 0.087 0.086 0.086



Fluorescein (uM) 10.00 5.00 2.5 1.25 0.625 0.313 0.156 0.078 0.039 0.0195 0.0098
Abs mean 6.461E6 3.551E6 1.900E6 9.253E5 4.844E5 2.420E5 1.162E5 5.755E4 3.016E4 1.527E4 7.731E3