Difference between revisions of "Team:HebrewU/InterLab"

 
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<html lang="en">
 
<html lang="en">
 
<head>
 
<head>
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<title>HebrewU Interlab </title>
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<title>HebrewU HujiGEM 2018</title>
 
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</style>
 
 
<body>
 
<body>
 +
<div class="w3-blue-gray">
 +
<ul id="ddmenu_hebrewu">
 +
            <li style="float:left;margin-top:12px;padding-left:15px;"><br />Hebrew</li>
  
−
<ul id="ddmenu_hebrewu">
+
    <div class="huji_homebutton">
−
+
           
−
    <div class="huji_homebutton" >
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    <li><a href=""https://2018.igem.org/Team:HebrewU">HebrewU</a></li>
+
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−
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+
        </a>
−
   
+
   
−
    <li><a href="https://2018.igem.org/Team:HebrewU/Project">Project</a>
+
        <a href="https://2018.igem.org/Team:HebrewU">
 +
                <img class="ulogo" src="https://static.igem.org/mediawiki/2018/d/d3/T--Hebrewu--huji_boiling_logo.png" width="60" height="60" style="margin-left:-27px;">
 +
                </a>
 +
  </div>
 +
 
 +
    <li><a>Project</a>
 
         <ul>
 
         <ul>
−
            <li><a href="https://2018.igem.org/Team:HebrewU/Motivation">Motivation</a></li>
 
 
             <li><a href="https://2018.igem.org/Team:HebrewU/Description">Description</a></li>
 
             <li><a href="https://2018.igem.org/Team:HebrewU/Description">Description</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Results">Results</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Model">Model</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/BioBricks">BioBricks</a></li>
+
            <li><a href="https://2018.igem.org/Team:HebrewU/Demonstrate">Results</a></li>
−
         </ul>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Parts">Parts</a></li>
−
               
+
            <li><a href="https://2018.igem.org/Team:HebrewU/Software">MOOLTi</a></li>
 +
 
 +
         </ul>      
 
     </li>
 
     </li>
−
    <li><a href="https://2018.igem.org/Team:HebrewU/Team">Team</a>
+
    <li><a>Lab</a>
 
         <ul>
 
         <ul>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Team">Members</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Design">Yeast Design</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Attribiutions">Attribiutions</a></li>
+
            <li><a href="https://2018.igem.org/Team:HebrewU/Plant">Plant Design</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Sponsors">Sponsors</a></li>
+
            <li><a href="https://2018.igem.org/Team:HebrewU/Notebook">Notebook</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Photo Gallery">Photo Gallery</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/InterLab">Inter-lab</a></li>
 +
             <li><a href="https://2018.igem.org/Team:HebrewU/Safety">Safety</a></li>
 +
             <li><a href="https://2018.igem.org/Team:HebrewU/Protocols">Protocols</a></li>
 
         </ul>
 
         </ul>
 +
       
 
     </li>
 
     </li>
 +
 
 
      
 
      
−
     <li><a href="https://2018.igem.org/Team:HebrewU/Human Practice">Human Practice</a>
+
     <li><a href="https://2018.igem.org/Team:HebrewU/Human_Practices">Human Practices</a>
 
         <ul>
 
         <ul>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Citizen Survey">Citizen Survey</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Ethics">Ethics</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Expert Interviews">Expert Interviews</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Global_aspects">Global Aspects</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Community">Community</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Public_engagment">Public Engagment</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Industry">Industry</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Open_Source">Open Source</a></li>
 
         </ul>
 
         </ul>
 
     </li>
 
     </li>
 
      
 
      
−
        <li><a href="https://2018.igem.org/Team:HebrewU/Our Lab">Our Lab</a>
+
    <li><a>Team</a>
 
         <ul>
 
         <ul>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Protocols">Protocols</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Team">Members</a></li>
−
            <li><a href="https://2018.igem.org/Team:HebrewU/Notebooks">Notebooks</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Attributions">Attributions</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/InterLab">Inter-lab</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Collaborations">Collaborations</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Safety">Safety</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Photo_Gallery">Photo Gallery</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Medal Criteria">Medal Criteria</a></li>
+
−
 
+
 
         </ul>
 
         </ul>
 
     </li>
 
     </li>
−
      
+
            <li ><a href="https://2018.igem.org/Team:HebrewU/Judging">Judging</a></li>
 +
           
 +
     <div class="social_huji">
 +
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 +
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 +
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 +
            <a href="https://www.igem.org/Main_Page"> <img class="social_icons" src="https://static.igem.org/mediawiki/2018/1/1a/T--Hebrewu--IGEM_logo.png" width="30"></a>
  
−
</ul>    
+
    </div>  
  
−
<div><br><br><div>
+
</ul>  
  
 +
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 +
<div class="huji_smallnav" id="huji_shownav">
 +
    <button class="huji_small_navbar">Project</button>
 +
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 +
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 +
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 +
            <a href="https://2018.igem.org/Team:HebrewU/Demonstrate"><button class="b_huji_small_subnav">Results</button></a>
 +
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 +
            <a href="https://2018.igem.org/Team:HebrewU/Software"><button class="b_huji_small_subnav">MOOLTi</button></a>
 +
        </div>
  
−
<div class="rowhuji">
+
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 +
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 +
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 +
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 +
            <a href="https://2018.igem.org/Team:HebrewU/Notebook"><button class="b_huji_small_subnav">Notebook</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/InterLab"><button class="b_huji_small_subnav">InterLab</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Safety"><button class="b_huji_small_subnav">Safety</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Protocols"><button class="b_huji_small_subnav">Protocols</button></a>
  
−
  <div class="columnhuji side">
+
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−
  </div>
+
−
 
+
−
  <div class="columnhuji middle">
+
−
  <div class="grid-container">
+
−
  <h2 class="ahuji"> Inter-Lab </h2>
+
−
  <p class="ahuji">
+
  
 +
    <button class="huji_small_navbar">Human Practice</button>
 +
        <div class="huji_small_navbar_panel">
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Universal and Standardized units are important in all disciplines of science, and especially in engineering. One important issue biology, and specifically genetic engineering faces today is a lack of standardized units. In synthetic biology we often employ marker proteins to assist in both real time assessment and in depth result interpretation. For these results to be consistent both between experiments, and between labs it is of paramount importance to create a standardized unit for said markers. Such a unit would also allow for the comparison of results from different labs and machines.
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The InterLab Study utilizes the wide access that iGEM has to biology teams all over the world. Taking advantage of this rare opportunity, the InterLab aims to improve the measurement tools available to both the iGEM community and the synthetic biology community as a whole. By measuring the fluorescence on a wide range of machines, in a wide range of labs, and regulating the results to a single control group, we hope to gather enough data to create this standard unit. As GFP is one of the most used markers today, this has been the focus of the InterLab studies over the past 5 years.
 
  
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Our team too believes in the need for a standardization of units with in the world of biology. We want to help make progress towards quantitative measurements between labs, and not be constrained to relative compassions. As such, were excited to take part in such a project and hope are data will support the progress of synthetic biology and genetic engineering.  
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Universal and standardized units are important in all disciplines of science. It allows students and professionals everywhere to understand the same language when describing substances or functions. However, the fields of biology and synthetic biology in particular often lack standardized units. For example, in genetic studies or modifications, we often employ marker proteins to assist in both real-time assessments and result interpretation. For results to be consistent between experiments and labs, it is of paramount importance to create a standardized unit for said markers. Such a unit would also allow for the precise comparison of results from different labs and machines, instead of relying on visual comparisons.  <br>
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     The InterLab Study utilizes iGEM’s broad network of biology students from around the world to improve the measurement tools available to the iGEM community and the synthetic biology community as a whole. By measuring GFP fluorescence on a wide range of machines in many different labs and relating the results to a single control group, we hope to gather enough data to create a standardized unit for it. Since GFP is one of the most frequently used markers today, this has been a major focus of the InterLab studies over the past 5 years.
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    Our team understands the need for unit standardization within the world of biology. We want to help make progress towards more precise quantitative measurements in biology research. As such, we are excited to take part in the Interlab Study and hope our data will support the progress of synthetic biology and genetic engineering.
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Latest revision as of 17:31, 12 December 2018

HebrewU HujiGEM 2018



Universal and standardized units are important in all disciplines of science. It allows students and professionals everywhere to understand the same language when describing substances or functions. However, the fields of biology and synthetic biology in particular often lack standardized units. For example, in genetic studies or modifications, we often employ marker proteins to assist in both real-time assessments and result interpretation. For results to be consistent between experiments and labs, it is of paramount importance to create a standardized unit for said markers. Such a unit would also allow for the precise comparison of results from different labs and machines, instead of relying on visual comparisons.  

The InterLab Study utilizes iGEM’s broad network of biology students from around the world to improve the measurement tools available to the iGEM community and the synthetic biology community as a whole. By measuring GFP fluorescence on a wide range of machines in many different labs and relating the results to a single control group, we hope to gather enough data to create a standardized unit for it. Since GFP is one of the most frequently used markers today, this has been a major focus of the InterLab studies over the past 5 years.  

Our team understands the need for unit standardization within the world of biology. We want to help make progress towards more precise quantitative measurements in biology research. As such, we are excited to take part in the Interlab Study and hope our data will support the progress of synthetic biology and genetic engineering.  




N. of Particles 2.35E8 1.18E8 5.88E7 2.94E7 1.47E7 7.35E6 3.68E6 1.84E6 9.19E5 4.60E5 2.30E5
Abs mean 0.977 0.568 0.305 0.195 0.139 0.113 0.098 0.092 0.087 0.086 0.086



Fluorescein (uM) 10.00 5.00 2.5 1.25 0.625 0.313 0.156 0.078 0.039 0.0195 0.0098
Abs mean 6.461E6 3.551E6 1.900E6 9.253E5 4.844E5 2.420E5 1.162E5 5.755E4 3.016E4 1.527E4 7.731E3