Difference between revisions of "Team:HebrewU/InterLab"

 
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<html>
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<html lang="en">
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<head>
 +
<title>HebrewU HujiGEM 2018</title>
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<meta charset="utf-8">
 +
<meta name="viewport" content="width=device-width, initial-scale=1">
  
−
<!--- Jquery script - remove when uploading to wiki --->
+
<!--- Own CSS --->
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<script src="https://ajax.googleapis.com/ajax/libs/jquery/3.3.1/jquery.min.js"></script>
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<link rel="stylesheet" href="https://2018.igem.org/Template:HebrewU/CSS?action=raw&ctype=text/css">
  
 
<!--- Main Menu script --->
 
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<script type="text/javascript" src="https://2018.igem.org/Template:Hebrewu/Javascript?action=raw&ctype=text/javascript"></script>
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<!--- w3 Css --->
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<link rel="stylesheet" href="https://2018.igem.org/Template:HebrewU/w3?action=raw&ctype=text/css">
  
 
<!--- Bootstrap Css --->
 
<!--- Bootstrap Css --->
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   <script src="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/js/bootstrap.min.js"></script>
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   <script src="https://2018.igem.org/Template:HebrewU/boot?action=raw&ctype=text/javascript"></script>
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  <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/3.3.7/css/bootstrap.min.css">
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<style>
 +
/* Disable I-GEM defult properties for blank page */
 +
    #sideMenu, #top_title {display:none;}
 +
    #sideMenu, #sideMenu *, #top_menu_inside, #top_menu_inside *{box-sizing: initial; -webkit-box-sizing: initial;}
 +
    #content { padding:0px; width:100%; margin-top:-12px; margin-left:0px;}
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    #body {background-color:#EDEADC; }
 +
    #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
 +
 +
    .w3-container {
 +
  overflow: auto;
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    }
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* {
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  -webkit-box-sizing:content-box ;
 +
    -moz-box-sizing:content-box ;
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<!--- Chart script --->
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<script type="text/javascript" src="https://www.gstatic.com/charts/loader.js"></script>
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.ahuji {
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    color:black
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<link rel="stylesheet" href="https://2018.igem.org/Template:HebrewU/CSS?action=raw&ctype=text/css">
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    }
−
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−
<!--- Footer font Css --->
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−
<link rel="stylesheet" href="http://maxcdn.bootstrapcdn.com/font-awesome/4.2.0/css/font-awesome.min.css">
+
 
 +
@media (max-width: 1000px) {
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    .fixed {
 +
    display: none;
 +
    }
 +
 
 +
.ulogo { display:none; }
 +
}
 +
 
 +
#panel, #flip_experts #panel_industry {
 +
    padding: 5px;
 +
    text-align: center;
 +
}
 +
 
 +
#panel panel_industry{
 +
    padding: 50px;
 +
    display: none;
 +
}
 +
 
 +
body {
 +
background-color:w3-blue-gray;
 +
 +
}
 +
 
 +
ul {
 +
 +
padding-left:150px;
 +
padding-right:100px;
 +
 
 +
 +
}
 +
 
 +
a {
 +
    color: hotpink;
 +
}
 +
 
 +
#HQ_page p {
 +
    font-family: Tahoma, Geneva, sans-serif;
 +
    font-size:20px;
 +
    color: white;
 +
}
 +
 
 +
a:visited {
 +
    color: white;
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}
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 +
 
 +
buttonhuji {
 +
font: 18px Calibri, Calibri;
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    border: none;
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    color: white;
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    padding: 10px 32px;
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    text-align: center;
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    display: inline-block;
 +
    font-size: 15px;
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    margin: 2px 2px;
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    -webkit-transition-duration: 0.4s; /* Safari */
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    transition-duration: 0.4s;
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    cursor: pointer;
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}
 +
 
 +
.buttonhuji1 {
 +
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    color: black;
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    border: 2px solid #829c8c;
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}
 +
 
 +
.buttonhuji1:hover {
 +
    background-color: #829c8c;
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    color: white;
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}
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 +
 
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.hujigem_showdata_button {
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  background-color:#829c8c;
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  font-size: 20px;
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  margin: 4px 2px;
 +
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 +
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 +
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  text-align:center
 +
 
 +
 
 +
}
 +
 
 +
.hujigem_showdata_button:hover {opacity: 0.9}
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 +
#hujigem_display_data{
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    width: 100%;
 +
    padding: 50px 0;
 +
    text-align: center;
 +
    background-color: lightblue;
 +
    margin-top: 20px;
 +
 
 +
}
 +
 
 +
#hujigem_showdata1,#hujigem_showdata2 {
 +
display:none;
 +
}
 +
 
 +
table_huji {
 +
    border-collapse: collapse;
 +
text-align: center;
 +
font-size-adjust:inherit;
 +
font-family:Calibri
 +
 
 +
}
 +
 
 +
th_huji, td_huji {
 +
    text-align: center;
 +
border: 1px solid #829c8c;
 +
    padding: 2px;
 +
    font-size-adjust:inherit;
 +
font-family:Calibri;
 +
 +
}
 +
 
 +
body {background-color: #EDEADC;}
 +
 
 +
 
 +
#tablevalues_hujigem {
 +
background-color:white;
 +
font-family:Calibri;
 +
    text-align: center;
 +
border: 1px solid #829c8c;
 +
 
 +
 
 +
 
 +
}
 +
 
 +
 
 +
</style>
  
 
<script>
 
<script>
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       var linearOptions = {
 
       var linearOptions = {
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         title: 'Particle Standard Curve (Linear Scale)',
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         legend: 'none',
 
         legend: 'none',
−
 
 
         width: 350,
 
         width: 350,
 
         height: 300,
 
         height: 300,
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       var linearOptions2 = {
 
       var linearOptions2 = {
−
         title: 'Fluorescein Standard Curve (Linear Scale)',
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         width: 350,
 
         width: 350,
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</script>
 
</script>
  
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<style>
 
−
.buttonhuji {
 
−
font: 18px Calibri, Calibri;
 
−
    border: none;
 
−
    color: white;
 
−
    padding: 10px 32px;
 
−
    text-align: center;
 
−
    display: inline-block;
 
−
    font-size: 15px;
 
−
    margin: 2px 2px;
 
−
    -webkit-transition-duration: 0.4s; /* Safari */
 
−
    transition-duration: 0.4s;
 
−
    cursor: pointer;
 
−
}
 
  
−
.buttonhuji1 {
+
<script>
−
     background-color: #fdfaf2;
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function myFunction() {
−
     color: black;
+
     var x = document.getElementById("Industry_display");
−
     border: 2px solid #829c8c;
+
     if (x.className.indexOf("w3-show") == -1) {
 +
        x.className += " w3-show";
 +
     } else {
 +
        x.className = x.className.replace(" w3-show", "");
 +
    }
 
}
 
}
 +
</script>
  
−
.buttonhuji1:hover {
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<script>
−
     background-color: #829c8c;
+
function myFunction_2() {
−
     color: white;
+
     var x = document.getElementById("Interviews_display");
 +
    if (x.className.indexOf("w3-show") == -1) {
 +
        x.className += " w3-show";
 +
     } else {
 +
        x.className = x.className.replace(" w3-show", "");
 +
    }
 
}
 
}
 +
</script>
  
 +
<body>
 +
<div class="w3-blue-gray">
 +
<ul id="ddmenu_hebrewu">
 +
            <li style="float:left;margin-top:12px;padding-left:15px;"><br />Hebrew</li>
  
−
.hujigem_showdata_button {
+
    <div class="huji_homebutton">
−
  background-color:#829c8c;
+
           
−
  border: none;
+
        <a href="javascript:void(0);" class="icon" onClick="show_small_nav()">
−
  color: white;
+
            <img src="https://static.igem.org/mediawiki/2018/d/dc/T--Hebrewu--huji_small_manu.png" width="20" height="20" padding:"2px">
−
  padding: 10px 10px;
+
        </a>
−
  text-align: center;
+
   
−
  font-size: 16px;
+
        <a href="https://2018.igem.org/Team:HebrewU">
−
  margin: 4px 2px;
+
                <img class="ulogo" src="https://static.igem.org/mediawiki/2018/d/d3/T--Hebrewu--huji_boiling_logo.png" width="60" height="60" style="margin-left:-27px;">
−
  opacity: 0.6;
+
                </a>
−
  transition: 0.3s;
+
  </div>  
−
  text-decoration: none;
+
 
−
  cursor: pointer;
+
     <li><a>Project</a>
−
 
+
−
 
+
−
}
+
−
 
+
−
.hujigem_showdata_button:hover {opacity: 0.9}
+
−
 
+
−
#hujigem_display_data{
+
−
    width: 100%;
+
−
    padding: 50px 0;
+
−
    text-align: center;
+
−
    background-color: lightblue;
+
−
    margin-top: 20px;
+
−
 
+
−
}
+
−
 
+
−
#hujigem_showdata1,#hujigem_showdata2 {
+
−
display:none;
+
−
}
+
−
 
+
−
table_huji {
+
−
    border-collapse: collapse;
+
−
text-align: center;
+
−
font-size-adjust:inherit;
+
−
font-family:Calibri
+
−
 
+
−
}
+
−
 
+
−
th_huji, td_huji {
+
−
    text-align: center;
+
−
border: 1px solid #829c8c;
+
−
    padding: 2px;
+
−
    font-size-adjust:inherit;
+
−
font-family:Calibri;
+
−
+
−
}
+
−
 
+
−
body {background-color: #EDEADC;}
+
−
 
+
−
 
+
−
#tablevalues_hujigem {
+
−
background-color:white;
+
−
font-family:Calibri;
+
−
    text-align: center;
+
−
border: 1px solid #829c8c;
+
−
 
+
−
 
+
−
 
+
−
}
+
−
 
+
−
</style>
+
−
<body bgcolor="#E6E6FA">
+
−
<div>
+
−
<ul id="ddmenu_hebrewu">
+
−
+
−
    <div class="huji_homebutton" >
+
−
    <li><img id="huji_logo" src="https://static.igem.org/mediawiki/2018/7/76/T--HebrewU--logo_animation.png" width="109" height="18"></li>
+
−
    </div>  
+
−
   
+
−
     <li><a href="https://2018.igem.org/Team:HebrewU/Project">Project</a>
+
 
         <ul>
 
         <ul>
−
            <li><a href="https://2018.igem.org/Team:HebrewU/Motivation">Motivation</a></li>
 
 
             <li><a href="https://2018.igem.org/Team:HebrewU/Description">Description</a></li>
 
             <li><a href="https://2018.igem.org/Team:HebrewU/Description">Description</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Results">Results</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Model">Model</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/BioBricks">BioBricks</a></li>
+
            <li><a href="https://2018.igem.org/Team:HebrewU/Demonstrate">Results</a></li>
−
         </ul>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Parts">Parts</a></li>
−
               
+
            <li><a href="https://2018.igem.org/Team:HebrewU/Software">MOOLTi</a></li>
 +
 
 +
         </ul>      
 
     </li>
 
     </li>
−
    <li><a href="https://2018.igem.org/Team:HebrewU/Team">Team</a>
+
    <li><a>Lab</a>
 
         <ul>
 
         <ul>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Team">Members</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Design">Yeast Design</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Attribiutions">Attribiutions</a></li>
+
            <li><a href="https://2018.igem.org/Team:HebrewU/Plant">Plant Design</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Sponsors">Sponsors</a></li>
+
            <li><a href="https://2018.igem.org/Team:HebrewU/Notebook">Notebook</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Photo Gallery">Photo Gallery</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/InterLab">Inter-lab</a></li>
 +
             <li><a href="https://2018.igem.org/Team:HebrewU/Safety">Safety</a></li>
 +
             <li><a href="https://2018.igem.org/Team:HebrewU/Protocols">Protocols</a></li>
 
         </ul>
 
         </ul>
 +
       
 
     </li>
 
     </li>
 +
 
 
      
 
      
−
     <li><a href="https://2018.igem.org/Team:HebrewU/Human Practice">Human Practice</a>
+
     <li><a href="https://2018.igem.org/Team:HebrewU/Human_Practices">Human Practices</a>
 
         <ul>
 
         <ul>
−
                    <li><a href="https://2018.igem.org/Team:HebrewU/Ethics">Ethics</a></li>
+
            <li><a href="https://2018.igem.org/Team:HebrewU/Ethics">Ethics</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Citizen Survey">Citizen Survey</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Global_aspects">Global Aspects</a></li>
−
            <li><a href="https://2018.igem.org/Team:HebrewU/Expert Interviews">Expert Interviews</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Public_engagment">Public Engagment</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Community">Community</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Open_Source">Open Source</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Industry">Industry</a></li>
+
 
         </ul>
 
         </ul>
 
     </li>
 
     </li>
 
      
 
      
−
        <li><a href="https://2018.igem.org/Team:HebrewU/Our Lab">Our Lab</a>
+
    <li><a>Team</a>
 
         <ul>
 
         <ul>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Protocols">Protocols</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Team">Members</a></li>
−
            <li><a href="https://2018.igem.org/Team:HebrewU/Notebooks">Notebooks</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Attributions">Attributions</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/InterLab">Inter-lab</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Collaborations">Collaborations</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Safety">Safety</a></li>
+
             <li><a href="https://2018.igem.org/Team:HebrewU/Photo_Gallery">Photo Gallery</a></li>
−
             <li><a href="https://2018.igem.org/Team:HebrewU/Medal Criteria">Medal Criteria</a></li>
+
−
 
+
 
         </ul>
 
         </ul>
−
       
 
 
     </li>
 
     </li>
 +
            <li ><a href="https://2018.igem.org/Team:HebrewU/Judging">Judging</a></li>
 +
           
 +
    <div class="social_huji">
 +
            <a href="https://twitter.com/GemHuji"> <img class="social_icons" src="https://static.igem.org/mediawiki/2018/3/39/T--Hebrewu--twitter_logo.png" width="25" height="25"></a>
 +
            <a href="https://www.facebook.com/HUJiGEM"> <img class="social_icons" src="https://static.igem.org/mediawiki/2018/8/8a/T--Hebrewu--facebook_logo.png" width="25" height="25"></a>
 +
            <a href="https://www.instagram.com/hujigem/"> <img class="social_icons" src="https://static.igem.org/mediawiki/2018/5/54/T--Hebrewu--insta_logo.png" width="25" height="25"></a>
 +
            <a href="https://www.igem.org/Main_Page"> <img class="social_icons" src="https://static.igem.org/mediawiki/2018/1/1a/T--Hebrewu--IGEM_logo.png" width="30"></a>
 +
 +
    </div> 
 +
 +
</ul>
 +
 +
<!----------- Responsive Navbar --------------->   
 +
<div class="huji_smallnav" id="huji_shownav">
 +
    <button class="huji_small_navbar">Project</button>
 +
        <div class="huji_small_navbar_panel">
 +
            <a href="https://2018.igem.org/Team:HebrewU/Description"><button class="b_huji_small_subnav">Description</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Model"><button class="b_huji_small_subnav">Model</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Demonstrate"><button class="b_huji_small_subnav">Results</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Parts"><button class="b_huji_small_subnav">Parts</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Software"><button class="b_huji_small_subnav">MOOLTi</button></a>
 +
        </div>
 +
 +
    <button class="huji_small_navbar">Lab</button>
 +
        <div class="huji_small_navbar_panel">
 +
            <a href="https://2018.igem.org/Team:HebrewU/Design"><button class="b_huji_small_subnav">Yeast Design</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Plant"><button class="b_huji_small_subnav">Plant Design</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Notebook"><button class="b_huji_small_subnav">Notebook</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/InterLab"><button class="b_huji_small_subnav">InterLab</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Safety"><button class="b_huji_small_subnav">Safety</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Protocols"><button class="b_huji_small_subnav">Protocols</button></a>
 +
 +
        </div>
 +
 +
    <button class="huji_small_navbar">Human Practice</button>
 +
        <div class="huji_small_navbar_panel">
 +
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Universal and standardized units are important in all disciplines of science. It allows students and professionals everywhere to understand the same language when describing substances or functions. However, the fields of biology and synthetic biology in particular often lack standardized units. For example, in genetic studies or modifications, we often employ marker proteins to assist in both real-time assessments and result interpretation. For results to be consistent between experiments and labs, it is of paramount importance to create a standardized unit for said markers. Such a unit would also allow for the precise comparison of results from different labs and machines, instead of relying on visual comparisons.  <br>
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  <p class="ahuji">Universal and standardized units are important in all disciplines of science. It allows for students and professionals everywhere to understand the same language when describing substances or functions. However, the fields of biology and synthetic biology in particular often lack standardized units. For example, in genetic studies or modifications, we often employ marker proteins to assist in both real-time assessments and result interpretation. For results to be consistent between experiments and labs, it is of paramount importance to create a standardized unit for said markers. Such a unit would also allow for the precise comparison of results from different labs and machines, instead of relying on visual comparisons.  <br>
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     The InterLab Study utilizes iGEM’s broad network of biology students from around the world to improve the measurement tools available to the iGEM community and the synthetic biology community as a whole. By measuring GFP fluorescence on a wide range of machines in many different labs and relating the results to a single control group, we hope to gather enough data to create a standardized unit for it. Since GFP is one of the most frequently used markers today, this has been a major focus of the InterLab studies over the past 5 years.  
 
     The InterLab Study utilizes iGEM’s broad network of biology students from around the world to improve the measurement tools available to the iGEM community and the synthetic biology community as a whole. By measuring GFP fluorescence on a wide range of machines in many different labs and relating the results to a single control group, we hope to gather enough data to create a standardized unit for it. Since GFP is one of the most frequently used markers today, this has been a major focus of the InterLab studies over the past 5 years.  
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Latest revision as of 17:31, 12 December 2018

HebrewU HujiGEM 2018



Universal and standardized units are important in all disciplines of science. It allows students and professionals everywhere to understand the same language when describing substances or functions. However, the fields of biology and synthetic biology in particular often lack standardized units. For example, in genetic studies or modifications, we often employ marker proteins to assist in both real-time assessments and result interpretation. For results to be consistent between experiments and labs, it is of paramount importance to create a standardized unit for said markers. Such a unit would also allow for the precise comparison of results from different labs and machines, instead of relying on visual comparisons.  

The InterLab Study utilizes iGEM’s broad network of biology students from around the world to improve the measurement tools available to the iGEM community and the synthetic biology community as a whole. By measuring GFP fluorescence on a wide range of machines in many different labs and relating the results to a single control group, we hope to gather enough data to create a standardized unit for it. Since GFP is one of the most frequently used markers today, this has been a major focus of the InterLab studies over the past 5 years.  

Our team understands the need for unit standardization within the world of biology. We want to help make progress towards more precise quantitative measurements in biology research. As such, we are excited to take part in the Interlab Study and hope our data will support the progress of synthetic biology and genetic engineering.  




N. of Particles 2.35E8 1.18E8 5.88E7 2.94E7 1.47E7 7.35E6 3.68E6 1.84E6 9.19E5 4.60E5 2.30E5
Abs mean 0.977 0.568 0.305 0.195 0.139 0.113 0.098 0.092 0.087 0.086 0.086



Fluorescein (uM) 10.00 5.00 2.5 1.25 0.625 0.313 0.156 0.078 0.039 0.0195 0.0098
Abs mean 6.461E6 3.551E6 1.900E6 9.253E5 4.844E5 2.420E5 1.162E5 5.755E4 3.016E4 1.527E4 7.731E3