Difference between revisions of "Team:HebrewU/InterLab"

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{{HebrewU}}
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<html lang="en">
<html>
+
<head>
 +
<title>HebrewU HujiGEM 2018</title>
 +
<meta charset="utf-8">
 +
<meta name="viewport" content="width=device-width, initial-scale=1">
  
 +
<!--- Own CSS --->
 +
<link rel="stylesheet" href="https://2018.igem.org/Template:HebrewU/CSS?action=raw&ctype=text/css">
  
<div class="column full_size judges-will-not-evaluate">
+
<!--- Main Menu script --->
<h3> ALERT! </h3>
+
<script type="text/javascript" src="https://2018.igem.org/Template:Hebrewu/Javascript?action=raw&ctype=text/javascript"></script>
<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
+
 
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
<!--- w3 Css --->
 +
<link rel="stylesheet" href="https://2018.igem.org/Template:HebrewU/w3?action=raw&ctype=text/css">
 +
 
 +
<!--- Bootstrap Css --->
 +
  <script src="https://2018.igem.org/Template:HebrewU/boot?action=raw&ctype=text/javascript"></script>
 +
  <link rel="stylesheet" href="https://2018.igem.org/Template:HebrewU/strap?action=raw&ctype=text/css">
 +
 
 +
<style>
 +
/* Disable I-GEM defult properties for blank page */
 +
    #sideMenu, #top_title {display:none;}
 +
    #sideMenu, #sideMenu *, #top_menu_inside, #top_menu_inside *{box-sizing: initial; -webkit-box-sizing: initial;}
 +
    #content { padding:0px; width:100%; margin-top:-12px; margin-left:0px;}
 +
    #body {background-color:#EDEADC; }
 +
    #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
 +
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    .w3-container {
 +
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  -webkit-box-sizing:content-box ;
 +
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 +
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 +
 
 +
.ahuji {
 +
    color:black
 +
    }
 +
 
 +
 
 +
 
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@media (max-width: 1000px) {
 +
    .fixed {
 +
    display: none;
 +
    }
 +
 
 +
.ulogo { display:none; }
 +
}
 +
 
 +
#panel, #flip_experts #panel_industry {
 +
    padding: 5px;
 +
    text-align: center;
 +
}
 +
 
 +
#panel panel_industry{
 +
    padding: 50px;
 +
    display: none;
 +
}
 +
 
 +
body {
 +
background-color:w3-blue-gray;
 +
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}
 +
 
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ul {
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padding-left:150px;
 +
padding-right:100px;
 +
 
 +
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}
 +
 
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a {
 +
    color: hotpink;
 +
}
 +
 
 +
#HQ_page p {
 +
    font-family: Tahoma, Geneva, sans-serif;
 +
    font-size:20px;
 +
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}
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a:visited {
 +
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}
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buttonhuji {
 +
font: 18px Calibri, Calibri;
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    transition-duration: 0.4s;
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}
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.buttonhuji1 {
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    border: 2px solid #829c8c;
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}
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.buttonhuji1:hover {
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    background-color: #829c8c;
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}
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.hujigem_showdata_button {
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  background-color:#829c8c;
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  text-align:center
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}
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.hujigem_showdata_button:hover {opacity: 0.9}
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#hujigem_display_data{
 +
    width: 100%;
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    padding: 50px 0;
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    text-align: center;
 +
    background-color: lightblue;
 +
    margin-top: 20px;
 +
 
 +
}
 +
 
 +
#hujigem_showdata1,#hujigem_showdata2 {
 +
display:none;
 +
}
 +
 
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table_huji {
 +
    border-collapse: collapse;
 +
text-align: center;
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font-size-adjust:inherit;
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font-family:Calibri
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}
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th_huji, td_huji {
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border: 1px solid #829c8c;
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 +
font-family:Calibri;
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}
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 +
body {background-color: #EDEADC;}
 +
 
 +
 
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#tablevalues_hujigem {
 +
background-color:white;
 +
font-family:Calibri;
 +
    text-align: center;
 +
border: 1px solid #829c8c;
 +
 
 +
 
 +
 
 +
}
 +
 
 +
 
 +
</style>
 +
 
 +
<script>
 +
google.charts.load('current', {'packages':['corechart', 'line']});
 +
    google.charts.setOnLoadCallback(drawChart1);
 +
google.charts.setOnLoadCallback(drawChart2);
 +
 
 +
  <!--- Graph 1 --->
 +
 
 +
    function drawChart1() {
 +
 
 +
      var data = new google.visualization.DataTable();
 +
      data.addColumn('number', 'Pcounts');
 +
      data.addColumn('number', 'Abs');
 +
 
 +
      data.addRows([
 +
          [ 2.35E+08, 0.977],
 +
          [ 1.18E+08, 0.568],
 +
          [ 5.88E+07, 0.305],
 +
          [ 2.94E+07, 0.195],
 +
          [ 1.47E+07, 0.139],
 +
          [ 7.35E+06, 0.113],
 +
          [ 3.68E+06, 0.098],
 +
          [ 1.84E+06, 0.092],
 +
          [ 9.19E+05, 0.086],
 +
          [ 4.60E+05, 0.087],
 +
          [ 2.30E+05, 0.086],
 +
      ]);
 +
 
 +
      var linearOptions = {
 +
        title: 'Particle Standard Curve (Linear Scale), R^2 = 0.9986',
 +
        legend: 'none',
 +
        width: 350,
 +
        height: 300,
 +
pointSize: 12,
 +
        dataOpacity: 0.3,
 +
        colors: ['black'],
 +
        backgroundColor: '#edeadc',
 +
        hAxis: {
 +
          title: 'Particle Count / 100 uL',
 +
  format: 'scientific'
 +
 
 +
 
 +
        },
 +
        vAxis: {
 +
          title: 'Abs 600',
 +
          ticks: [0, 0.2, 0.4, 0.6, 0.8, 1, 1.2]
 +
        }
 +
 +
      };
 +
 
 +
      var logOptions = {
 +
        title: 'Particle Standard Curve (Log Scale)',
 +
        legend: 'none',
 +
        width: 350,
 +
        height: 300,
 +
pointSize: 12,
 +
        dataOpacity: 0.3,
 +
        colors: ['black'],
 +
        backgroundColor: '#edeadc',
 +
        hAxis: {
 +
          title: 'Particle Count / 100 uL',
 +
  format: 'scientific',
 +
          ticks: [1E5, 1E6, 1E7, 1E8]
 +
 
 +
        },
 +
        vAxis: {
 +
          title: 'Abs 600',
 +
          scaleType: 'log',
 +
          ticks: [0.01, 0.1, 1]
 +
        }
 +
 +
      };
 +
 
 +
      var linearChart = new google.visualization.LineChart(document.getElementById('linear_div'));
 +
      linearChart.draw(data, linearOptions);
 +
 
 +
      var logChart = new google.visualization.LineChart(document.getElementById('log_div'));
 +
      logChart.draw(data, logOptions);
 +
    }
 +
 +
<!--- Graph 2 information--->
 +
 
 +
function drawChart2() {
 +
 
 +
      var data = new google.visualization.DataTable();
 +
      data.addColumn('number', 'Pcounts');
 +
      data.addColumn('number', 'Abs');
 +
 
 +
      data.addRows([
 +
          [10.00, 6.461E+06],
 +
          [5.00, 3.551E+06],
 +
          [2.5, 1.900E+06],
 +
          [1.25, 9.253E+05],
 +
          [0.625, 4.844E+05],
 +
          [0.313, 2.420E+05],
 +
          [0.156, 1.162E+05],
 +
          [0.078, 5.755E+04],
 +
          [0.039, 3.016E+04],
 +
          [0.0195,1.527E+04],
 +
          [0.0098,7.731E+03],
 +
      ]);
 +
 
 +
 +
 +
 +
 +
      var linearOptions2 = {
 +
        title: 'Fluorescein Standard Curve (Linear Scale), R² = 0.997',
 +
        legend: 'none',
 +
        width: 350,
 +
        height: 300,
 +
pointSize: 12,
 +
        dataOpacity: 0.3,
 +
        colors: ['black'],
 +
        backgroundColor: '#edeadc',
 +
        hAxis: {
 +
          title: 'Fluorescein Concentration (uM)',
 +
  ticks: [0,2,4,6,8,10]
 +
 
 +
        },
 +
        vAxis: {
 +
          title: 'Fluorescence',
 +
 
 +
        }
 +
 +
      };
 +
 
 +
      var logOptions2 = {
 +
        title: 'Fluorescein Standard Curve (Log Scale)',
 +
        legend: 'none',
 +
        width: 350,
 +
        height: 300,
 +
pointSize: 12,
 +
        dataOpacity: 0.3,
 +
        colors: ['black'],
 +
        backgroundColor: '#edeadc',
 +
        hAxis: {
 +
        title: 'Fluorescein Concentration (uM)',
 +
ticks: [0.01, 0.1, 1, 10]
 +
 
 +
 
 +
        },
 +
        vAxis: {
 +
          title: 'Fluorescence',
 +
          scaleType: 'log',
 +
  format: 'scientific',
 +
  ticks: [1E5, 2E6, 3E7, 4E8, 5E8, 6E8, 7E8, 8E8]
 +
 
 +
        }
 +
 +
      };
 +
 
 +
      var linearChart2 = new google.visualization.LineChart(document.getElementById('linear_div2'));
 +
      linearChart2.draw(data, linearOptions2);
 +
 
 +
      var logChart2 = new google.visualization.LineChart(document.getElementById('log_div2'));
 +
      logChart2.draw(data, logOptions2);
 +
    }
 +
 +
 +
 +
</script>
 +
 
 +
 
 +
<script>
 +
function myFunction() {
 +
    var x = document.getElementById("Industry_display");
 +
    if (x.className.indexOf("w3-show") == -1) {
 +
        x.className += " w3-show";
 +
    } else {
 +
        x.className = x.className.replace(" w3-show", "");
 +
    }
 +
}
 +
</script>
 +
 
 +
<script>
 +
function myFunction_2() {
 +
    var x = document.getElementById("Interviews_display");
 +
    if (x.className.indexOf("w3-show") == -1) {
 +
        x.className += " w3-show";
 +
    } else {
 +
        x.className = x.className.replace(" w3-show", "");
 +
    }
 +
}
 +
</script>
 +
 
 +
<body>
 +
<div class="w3-blue-gray">
 +
<ul id="ddmenu_hebrewu">
 +
            <li style="float:left;margin-top:12px;padding-left:15px;"><br />Hebrew</li>
 +
 
 +
    <div class="huji_homebutton">
 +
           
 +
        <a href="javascript:void(0);" class="icon" onClick="show_small_nav()">
 +
            <img src="https://static.igem.org/mediawiki/2018/d/dc/T--Hebrewu--huji_small_manu.png" width="20" height="20" padding:"2px">
 +
        </a>
 +
   
 +
        <a href="https://2018.igem.org/Team:HebrewU">
 +
                <img class="ulogo" src="https://static.igem.org/mediawiki/2018/d/d3/T--Hebrewu--huji_boiling_logo.png" width="60" height="60" style="margin-left:-27px;">
 +
                </a>
 +
  </div>
 +
 
 +
    <li><a>Project</a>
 +
        <ul>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Description">Description</a></li>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Model">Model</a></li>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Demonstrate">Results</a></li>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Parts">Parts</a></li>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Software">MOOLTi</a></li>
 +
 
 +
        </ul>       
 +
    </li>
 +
    <li><a>Lab</a>
 +
        <ul>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Design">Yeast Design</a></li>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Plant">Plant Design</a></li>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Notebook">Notebook</a></li>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/InterLab">Inter-lab</a></li>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Safety">Safety</a></li>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Protocols">Protocols</a></li>
 +
        </ul>
 +
       
 +
    </li>
 +
 
 +
   
 +
    <li><a href="https://2018.igem.org/Team:HebrewU/Human_Practices">Human Practices</a>
 +
        <ul>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Ethics">Ethics</a></li>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Global_aspects">Global Aspects</a></li>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Public_engagment">Public Engagment</a></li>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Open_Source">Open Source</a></li>
 +
        </ul>
 +
    </li>
 +
   
 +
    <li><a>Team</a>
 +
        <ul>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Team">Members</a></li>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Attributions">Attributions</a></li>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Collaborations">Collaborations</a></li>
 +
            <li><a href="https://2018.igem.org/Team:HebrewU/Photo_Gallery">Photo Gallery</a></li>
 +
        </ul>
 +
    </li>
 +
            <li ><a href="https://2018.igem.org/Team:HebrewU/Judging">Judging</a></li>
 +
           
 +
    <div class="social_huji">
 +
            <a href="https://twitter.com/GemHuji"> <img class="social_icons" src="https://static.igem.org/mediawiki/2018/3/39/T--Hebrewu--twitter_logo.png" width="25" height="25"></a>
 +
            <a href="https://www.facebook.com/HUJiGEM"> <img class="social_icons" src="https://static.igem.org/mediawiki/2018/8/8a/T--Hebrewu--facebook_logo.png" width="25" height="25"></a>
 +
            <a href="https://www.instagram.com/hujigem/"> <img class="social_icons" src="https://static.igem.org/mediawiki/2018/5/54/T--Hebrewu--insta_logo.png" width="25" height="25"></a>
 +
            <a href="https://www.igem.org/Main_Page"> <img class="social_icons" src="https://static.igem.org/mediawiki/2018/1/1a/T--Hebrewu--IGEM_logo.png" width="30"></a>
 +
 
 +
    </div> 
 +
 
 +
</ul>
 +
 
 +
<!----------- Responsive Navbar --------------->   
 +
<div class="huji_smallnav" id="huji_shownav">
 +
    <button class="huji_small_navbar">Project</button>
 +
        <div class="huji_small_navbar_panel">
 +
            <a href="https://2018.igem.org/Team:HebrewU/Description"><button class="b_huji_small_subnav">Description</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Model"><button class="b_huji_small_subnav">Model</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Demonstrate"><button class="b_huji_small_subnav">Results</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Parts"><button class="b_huji_small_subnav">Parts</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Software"><button class="b_huji_small_subnav">MOOLTi</button></a>
 +
        </div>
 +
 
 +
    <button class="huji_small_navbar">Lab</button>
 +
        <div class="huji_small_navbar_panel">
 +
            <a href="https://2018.igem.org/Team:HebrewU/Design"><button class="b_huji_small_subnav">Yeast Design</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Plant"><button class="b_huji_small_subnav">Plant Design</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Notebook"><button class="b_huji_small_subnav">Notebook</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/InterLab"><button class="b_huji_small_subnav">InterLab</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Safety"><button class="b_huji_small_subnav">Safety</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Protocols"><button class="b_huji_small_subnav">Protocols</button></a>
 +
 
 +
        </div>
 +
 
 +
    <button class="huji_small_navbar">Human Practice</button>
 +
        <div class="huji_small_navbar_panel">
 +
            <a href="https://2018.igem.org/Team:HebrewU/Human_Practices"><button class="b_huji_small_subnav">Human Practice - Main Page</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Ethics"><button class="b_huji_small_subnav">Ethics</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Global_aspects"><button class="b_huji_small_subnav">Global Aspects</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Public_Engagment"><button class="b_huji_small_subnav">Public Engagment</button></a>
 +
            <a href="https://2018.igem.org/Team:HebrewU/Open_Source"><button class="b_huji_small_subnav">Open Source</button></a>
 +
        </div>
 +
 
 +
 
 +
     
 +
          <button class="huji_small_navbar">Team</button>
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            <a href="https://2018.igem.org/Team:HebrewU/Members"><button class="b_huji_small_subnav">Members</button></a>
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            <a href="https://2018.igem.org/Team:HebrewU/Attributions"><button class="b_huji_small_subnav">Attributions</button></a>
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            <a href="https://2018.igem.org/Team:HebrewU/Collaborations"><button class="b_huji_small_subnav">Collaborations</button></a>
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            <a href="https://2018.igem.org/Team:HebrewU/Judging"><button class="huji_small_navbar">Judging</button></a>
 
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    <a onClick="topFunction()" id="myBtn_up" title="Go to top"><img src="https://static.igem.org/mediawiki/2018/1/1e/T--hebrewu--arrow_up.png" width="40px" /></a>
  
<div class="clear"></div>
 
  
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<h1>InterLab</h1>
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
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For teams participating in the <a href="https://2018.igem.org/Measurement/InterLab">InterLab study</a>, all work must be shown on this page.
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 +
</div> 
 +
<!--- Page content start --->
 +
        <p style="padding-left:150px;padding-right:150px;text-align:justify;line-height:1.5">
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Universal and standardized units are important in all disciplines of science. It allows students and professionals everywhere to understand the same language when describing substances or functions. However, the fields of biology and synthetic biology in particular often lack standardized units. For example, in genetic studies or modifications, we often employ marker proteins to assist in both real-time assessments and result interpretation. For results to be consistent between experiments and labs, it is of paramount importance to create a standardized unit for said markers. Such a unit would also allow for the precise comparison of results from different labs and machines, instead of relying on visual comparisons.  <br>
 +
    <br>
 +
    The InterLab Study utilizes iGEM’s broad network of biology students from around the world to improve the measurement tools available to the iGEM community and the synthetic biology community as a whole. By measuring GFP fluorescence on a wide range of machines in many different labs and relating the results to a single control group, we hope to gather enough data to create a standardized unit for it. Since GFP is one of the most frequently used markers today, this has been a major focus of the InterLab studies over the past 5 years.
 +
 <br>
 +
    <br>
 +
    Our team understands the need for unit standardization within the world of biology. We want to help make progress towards more precise quantitative measurements in biology research. As such, we are excited to take part in the Interlab Study and hope our data will support the progress of synthetic biology and genetic engineering.
 +
 
 +
 
 +
  <div align="center"><br />
 +
    <img src="https://static.igem.org/mediawiki/2018/6/63/T--Hebrewu--interlab_chart1.png" width="70%">
 +
    <br /> <br />
 +
    <button onClick="myFunction()" class="hujigem_showdata_button" style>Raw data 1</button>
 +
 
 +
<div id="hujigem_showdata1" style="overflow-x:auto;">
 +
<table id="tablevalues_hujigem">
 +
 
 +
  <tr>
 +
    <th>N. of Particles</th>
 +
    <td>2.35E8</td>
 +
    <td>1.18E8</td>
 +
    <td>5.88E7</td>
 +
    <td>2.94E7</td>
 +
    <td>1.47E7</td>
 +
    <td>7.35E6</td>
 +
    <td>3.68E6</td>
 +
    <td>1.84E6</td>
 +
    <td>9.19E5</td>
 +
    <td>4.60E5</td>
 +
    <td>2.30E5</td>
 +
 
 +
 
 +
  </tr>
 +
  <tr>
 +
    <th>Abs mean</th>
 +
    <td>0.977</td>
 +
    <td>0.568</td>
 +
    <td>0.305</td>
 +
    <td>0.195</td>
 +
    <td>0.139</td>
 +
    <td>0.113</td>
 +
    <td>0.098</td>
 +
    <td>0.092</td>
 +
    <td>0.087</td>
 +
    <td>0.086</td>
 +
    <td>0.086</td>
 +
 
 +
 
 +
   
 +
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 +
  <tr>
 +
   
 +
</table>
 
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</div>
  
  
  
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}
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 +
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<img src="https://static.igem.org/mediawiki/2018/1/19/T--Hebrewu--interlab_chart2.png" width="70%">
 +
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 +
 +
 +
 +
 +
 +
<button onClick="myFunction2()" class="hujigem_showdata_button">Raw data 2</button>
 +
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 +
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 +
<table id="tablevalues_hujigem">
 +
 +
  <tr>
 +
    <th>Fluorescein (uM)</th>
 +
    <td>10.00</td>
 +
    <td>5.00</td>
 +
    <td>2.5</td>
 +
    <td>1.25</td>
 +
    <td>0.625</td>
 +
    <td>0.313</td>
 +
    <td>0.156</td>
 +
    <td>0.078</td>
 +
    <td>0.039</td>
 +
    <td>0.0195</td>
 +
    <td>0.0098</td>
 +
 +
 +
  </tr>
 +
  <tr>
 +
    <th>Abs mean</th>
 +
    <td>6.461E6</td>
 +
    <td>3.551E6</td>
 +
    <td>1.900E6</td>
 +
    <td>9.253E5</td>
 +
    <td>4.844E5</td>
 +
    <td>2.420E5</td>
 +
    <td>1.162E5</td>
 +
    <td>5.755E4</td>
 +
    <td>3.016E4</td>
 +
    <td>1.527E4</td>
 +
    <td>7.731E3</td>
 +
 +
 +
   
 +
  </tr>
 +
  <tr>
 +
   
 +
</table>
 +
</div>
 +
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 +
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</html>

Latest revision as of 17:31, 12 December 2018

HebrewU HujiGEM 2018



Universal and standardized units are important in all disciplines of science. It allows students and professionals everywhere to understand the same language when describing substances or functions. However, the fields of biology and synthetic biology in particular often lack standardized units. For example, in genetic studies or modifications, we often employ marker proteins to assist in both real-time assessments and result interpretation. For results to be consistent between experiments and labs, it is of paramount importance to create a standardized unit for said markers. Such a unit would also allow for the precise comparison of results from different labs and machines, instead of relying on visual comparisons.  

The InterLab Study utilizes iGEM’s broad network of biology students from around the world to improve the measurement tools available to the iGEM community and the synthetic biology community as a whole. By measuring GFP fluorescence on a wide range of machines in many different labs and relating the results to a single control group, we hope to gather enough data to create a standardized unit for it. Since GFP is one of the most frequently used markers today, this has been a major focus of the InterLab studies over the past 5 years.  

Our team understands the need for unit standardization within the world of biology. We want to help make progress towards more precise quantitative measurements in biology research. As such, we are excited to take part in the Interlab Study and hope our data will support the progress of synthetic biology and genetic engineering.  




N. of Particles 2.35E8 1.18E8 5.88E7 2.94E7 1.47E7 7.35E6 3.68E6 1.84E6 9.19E5 4.60E5 2.30E5
Abs mean 0.977 0.568 0.305 0.195 0.139 0.113 0.098 0.092 0.087 0.086 0.086



Fluorescein (uM) 10.00 5.00 2.5 1.25 0.625 0.313 0.156 0.078 0.039 0.0195 0.0098
Abs mean 6.461E6 3.551E6 1.900E6 9.253E5 4.844E5 2.420E5 1.162E5 5.755E4 3.016E4 1.527E4 7.731E3