Difference between revisions of "Team:WashU StLouis/Daily"
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| − | <p><strong><u> | + | <p><strong><u>Sunday, July 29</u></strong></p> |
<p><em>Present: Micah, Collin</em></p> | <p><em>Present: Micah, Collin</em></p> | ||
<p><strong> Lab Work </strong></p> | <p><strong> Lab Work </strong></p> | ||
| − | < | + | <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> |
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| − | <p><strong><u> | + | <p><strong><u>Monday, July 30</u></strong></p> |
| − | <p><em></em></p> | + | <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> |
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| − | <p><strong><u> | + | <p><strong><u>Tuesday, July 31</u></strong></p> |
| − | + | <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> | |
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| − | <p><strong><u> | + | <p><strong><u>Wednesday, August 1</u></strong></p> |
| − | <p><em>Present: | + | <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> |
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| − | <p><strong><u> | + | <p><strong><u>Thursday, August 2</u></strong></p> |
| − | <p><em>Present: | + | <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> |
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| − | <p><strong><u> | + | <p><strong><u>Friday, August 3</u></strong></p> |
| − | <p><em>Present: | + | <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> |
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| − | <p><strong><u> | + | <p><strong><u>Saturday, August 4</u></strong></p> |
| − | <p><em>Present: | + | <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> |
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| − | <p><strong><u> | + | <p><strong><u>Sunday, August 5</u></strong></p> |
| − | <p><em>Present: | + | <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> |
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| − | <p><strong><u> | + | <p><strong><u>Monday, August 6</u></strong></p> |
| − | <p><em>Present: | + | <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> |
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| − | <p><strong><u> | + | <p><strong><u>Tuesday, August 7</u></strong></p> |
| − | <p><em>Present: | + | <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> |
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| − | <p><strong><u> | + | <p><strong><u>Wednesday, August 8</u></strong></p> |
| − | <p><em>Present: | + | <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p> |
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| − | <p><strong><u> | + | <p><strong><u>Thursday, August 9</u></strong></p> |
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| − | <p><strong><u> | + | <p><strong><u>Friday, August 10</u></strong></p> |
| − | <p><em>Present: | + | <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p> |
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| − | <p><strong><u> | + | <p><strong><u>Saturday, August 11</u></strong></p> |
| − | <p><em>Present: | + | <p><em>Present: Cam, Diva, Elizabeth, Havisha, Kyle</em></p> |
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Revision as of 14:46, 18 July 2018
| June 2018 | ||||||
|---|---|---|---|---|---|---|
| Sunday | Monday | Tuesday | Wednesday | Thursday | Friday | Saturday |
| 1 | 2 | |||||
| 3 | 4 | 5 | 6 | 7 | 8 | 9 |
| 10 | 11 | 12 | 13 | 14 | 15 | 16 |
| 17 | 18 | 19 | 20 | 21 | 22 | 23 |
| 24 | 25 | 26 | 27 | 28 | 29 | 30 |
Friday, June 1
No work done today!
Saturday, June 2
No work done today!
Sunday, June 3
No work done today!
Monday, June 4
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- Researched different resistance genes to identify ones that are sequenced and applicable to the project [Elizabeth, Diva, Cam]
- Studied glucosamine detection mechanisms to signal the presence the presence of fungi [Havisha]
- Planned trip to Uganda and human practices potential abroad [Kyle]
Tuesday, June 5
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- Researched different resistance genes to identify ones that are sequenced and applicable to the project [Elizabeth, Diva]
- Compared different aspects of Sr genes [Cam]
- Examined a protocol for rapid cloning of plant disease resistance genes [Diva]
- Studied glucosamine detection mechanisms to signal the presence the presence of fungi [Havisha, Diva]
- Planned trip to Uganda and potential human practices abroad [Kyle]
Wednesday, June 6
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- Examined lengths of resistance gene sequences to begin ordering material [Elizabeth]
- Studied fungal spore traps to limit specificity to Puccinia graminis [Havisha]
- Investigated different detection and reporting mechanisms for reporting information with a device [Cam, Diva]
- Planned trip to Uganda and potential human practices abroad [Kyle]
Thursday, June 7
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- Met with Dr. Brennan to redefine project goals and timeline [Cam, Diva, Elizabeth, Havisha, Kyle]
- Reviewed videos from the BGRI to determine locations of wheat rust fungus prevalence [Elizabeth]
- Prepared presentation for Microsoft partnership meeting and consulted Dr. Brennan on areas for improvement [Cam]
- Studied fungal spore traps to limit specificity to Puccinia graminis [Havisha]
- Continued research on various detection mechanisms [Diva]
- Planned trip to Uganda and potential human practices abroad [Kyle]
Friday, June 8
Present: Cam, Diva, Elizabeth, Havisha
- Met with a representative from Microsoft to establish a technology partnership [Cam]
- Reviewed papers regarding resistance gene interactions (Sr35, Sr22, and Sr45) [Elizabeth]
- Considered phage related detection mechanisms, alongside the use of CRISPR/CAS9 [Diva]
- Studied fungal spore traps to select only ones of Puccinia graminis [Havisha]
Saturday, June 9
No work done today!
Sunday, June 10
No work done today!
Monday, June 11
Present: Cam, Diva, Elizabeth, Havisha
- Prepared a summary of the global impact of rust for Microsoft [Cam]
- Planned for the use of Sr33 and Sr13 [Elizabeth]
- Studied compounds unique to germinated P. graminis and infectious structures [Havisha]
- Investigated the DNA repair mechanism of eukaryotes (fungi in particular) [Diva]
Tuesday, June 12
Present: Cam, Diva, Elizabeth, Havisha
- Revised our project abstract to incorporate changes made over the past week [Cam, Diva, Elizabeth, Havisha]
- Conducted a marketing brainstorming session [Cam, Diva, Elizabeth, Havisha]
- Researched Sr50 and truncating resistance genes [Elizabeth]
- Compiled a research summary for Microsoft [Cam]
- Examined different detection mechanisms for ribitol [Havisha]
- Investigated the DNA repair mechanism of eukaryotes (fungi in particular) [Diva]
Wednesday, June 13
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- Met with Dr. Brennan to assess abstract and project design in preparation to meet with Monsanto [Cam, Diva, Elizabeth, Kyle, Havisha]
- Compiled gene sequences needed for the project [Elizabeth]
- Began developing the wiki [Havisha]
- Identified the sequence for a gene induced by ribotin to transfer into a plasmid and transform E. coli with [Havisha]
- Investigated the DNA repair mechanism of eukaryotes (fungi in particular) [Diva]
- Planned trip to Uganda and potential human practices abroad [Kyle]
Thursday, June 14
Present: Cam, Diva, Elizabeth, Havisha
- Connected with many iGEM teams through social media [Elizabeth, Cam]
- Developed the wiki template and daily notebook log pages [Havisha]
- Attempted to identify promoter sequences induced by ribotin [Diva]
- Investigated truncating Sr50 and fusion proteins [Elizabeth]
Friday, June 15
Present: Cam, Diva, Elizabeth, Havisha
- Developed footer and human practices page of the wiki [Havisha]
- Conducted a call with a grain farmer for fundraising and human practices efforts [Cam]
- Organized presentation in preparation for a meeting with Monsanto [Cam]
- Investigated Sr35 and fusion proteins [Elizabeth]
- Met with Jeff to discuss logistics of project [Havisha, Elizabeth]
Saturday, June 16
No work done today!
Sunday, June 17
No work done today!
Monday, June 18
Present: Cam, Diva, Elizabeth, Havisha
- Worked on presentation in preparation for meeting with Monsanto [Cam, Diva, Elizabeth, Havisha]
- Prepared PBS buffer and LB Agar plates in preparation for interlab [Cam, Diva, Elizabeth, Havisha]
- Developed technology partnership with Microsoft. [Cam]
Tuesday, June 19
Present: Cam, Diva, Elizabeth, Havisha
- Met with Dr. Brennan to prepare for Monsanto presentation. [Cam, Diva, Elizabeth, Havisha]
- Prepared LB buffer and began overnight culture. [Cam, Diva, Elizabeth, Havisha]
Control agar + CM plates made on 6/18
Wednesday, June 20
Present: Cam, Diva, Elizabeth, Havisha
- Prepared chemically competent DH5α E. coli cells [Cam, Diva, Elizabeth, Havisha]
- Developed list of protocols for the wiki [Havisha, Diva]
Thursday, June 21
Present: Cam, Diva, Elizabeth, Havisha
- Toured Monsanto and Pfizer facilities [Cam, Diva, Elizabeth, Havisha]
- Presented to Monsanto researchers to solicit advice and feedback from people in the field [Cam, Diva, Elizabeth, Havisha]
Friday, June 22
Present: Cam, Diva, Elizabeth, Havisha
- Identified gene sequences for ribitol metabolism [Havisha]
- Began investigating LAMP and primers [Diva]
- Studied protein-protein interactions of CC-NLR proteins [Diva, Elizabeth]
- Continued with fundraising efforts and coordinating partnership with Monsanto [Cam]
Saturday, June 23
No work done today!
Sunday, June 24
No work done today!
Monday, June 25
Present: Cam, Diva, Havisha
- Worked on Day 1 of the interlab study protocol [Cam, Diva, Havisha]
Tuesday, June 26
Present: Cam, Diva, Havisha
- Troubleshooted problems with Day 1 transformation [Cam, Diva, Havisha]
- Corresponded with Microsoft regarding digital lab notebook [Cam]
- Solidified gene sequences needed for general detection mechanism [Havisha]
- Began filling out iGEM safety form part 1 [Diva]
Transformed DH5α from interlab studies day one on 6/25
Wednesday, June 27
Present: Cam, Diva, Havisha
- Wrote project overview, background, and general detection mechanism summary for wiki [Havisha]
- Completed safety form [Diva, Havisha]
- Made LB Agar Media [Diva, Havisha]
- Started an overnight culture of DH5α [Diva, Havisha]
Thursday, June 28
Present: Diva, Elizabeth, Havisha
- Submitted safety form [Diva]
- Plated LB Agar plates [Diva, Elizabeth, Havisha]
- Made chemically competent DH5α [Diva, Elizabeth, Havisha]
- Developed banners for wiki pages [Havisha]
Friday, June 29
Present: Diva, Elizabeth, Havisha
- Worked on members’ introductions for the wiki [Diva, Elizabeth, Havisha]
- Tested competency of cells prepared on 6/28 [Diva, Elizabeth, Havisha]
Control agar plate made on 6/28
Saturday, June 30
No work done today!
Sunday, July 1
Present: Diva, Havisha
- Performed calibration protocol for interlab study [Diva, Havisha]
Control agar plate with non-transformed competent cells plated on 6/29
Agar + CM plates with cells transformed with iGEM test kit from 6/29
Monday, July 2
Present: Diva, Elizabeth, Havisha
- Made LB Agar + CM plates [Diva, Elizabeth, Havisha]
- Transformed E. coli DH5α for Interlab Protocol Day 1 [Diva, Elizabeth, Havisha]
Tuesday, July 3
Present: Diva, Elizabeth, Havisha
- Met with Jeff to discuss project goals and progress [Diva, Elizabeth, Havisha]
Wednesday, July 4
Present: Cam, Diva, Elizabeth
- Worked on Day 2 of interlab protocol [Cam, Diva, Elizabeth]
Thursday, July 5
Present: Cam, Diva, Elizabeth
- Worked on Day 3 of interlab protocol [Cam, Diva, Elizabeth]
- Informed of contamination with E. coli and materials [Cam, Diva, Elizabeth]
Friday, July 6
Present: Cam, Diva, Elizabeth, Kyle
- Made 1L LB Broth [Diva]
- Made LB Agar + CM plates [Cam, Elizabeth]
- Made TSS Buffer [Elizabeth, Diva]
- Looked into GPCRs [Kyle]
Saturday, July 7
Present: Cam, Elizabeth
- Transformed DH5α cells using competent cell test kit [Cam, Elizabeth]
Sunday, July 8
Present: Elizabeth
- Checked transformed cell plates for growth [Elizabeth]
Agar + CM plates with cells transformed with iGEM test kit from 7/7
Monday, July 9
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- Consulted with Microsoft computing experts on modelling and device [Cam]
- Finalized ribitol operon sequences to order [Havisha]
- Studied resistance genes and sequences to order [Elizabeth]
Tuesday, July 10
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- Met with Dr. Brennan to discuss project checkpoints and progress [Elizabeth, Havisha, Kyle]
- Made new LB media [Diva]
- Made and plated LB agar and LB agar + CM [Diva, Elizabeth, Havisha]
- Studied resistance genes and sequences to order [Elizabeth]
- Ordered ribitol operon gene sequences [Havisha]
- Met with Dr. Parikh and attended a lecture to develop international human practices [Kyle]
- Began DH5α overnight culture [Diva, Elizabeth, Havisha]
Wednesday, July 11
Present: Diva, Elizabeth, Havisha
- Plated DH5α cells on LB agar plate to check for plate contamination [Havisha]
- Checked plated DH5α cells [Diva, Elizabeth]
Agar plate with overnight culture of DH5α from 7/11
Thursday, July 12
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- Conducted Day 1 of interlab protocol with competent cells from NEB [Cam, Diva, Elizabeth, Havisha, Kyle]
- Discussed human practices progress and integration into project [Cam, Diva, Elizabeth, Havisha, Kyle]
- Checked plated transformed DH5α cells [Diva, Elizabeth]
Agar + CM plates with transformed cells from Day 1 of interlab protocol from 7/12
Friday, July 13
Present: Cam, Diva, Elizabeth, Kyle
- Conducted Day 2 of interlab protocol [Diva, Elizabeth]
- Met with Monsanto plant scientists to discuss project design and resistance genes [Diva, Elizabeth, Kyle]
- Conducted Hour 0 of Day 3 of interlab protocol [Cam, Diva, Elizabeth]
- Made LB Agar + CM plates [Cam, Diva]
Saturday, July 14
Present: Cam, Diva, Elizabeth, Kyle
- Conducted Hour 6 of Day 3 of interlab protocol [Diva, Elizabeth, Kyle]
- Made LB broth [Cam]
Sunday, July 15
No work done today!
Monday, July 16
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- Conducted a production meeting to redefine project progress and delegate individual tasks [Cam, Diva, Elizabeth, Havisha, Kyle]
- Compiling gene sequences for resistance genes [Elizabeth]
- Met with a representative from the Missouri Coalition for the Environment [Kyle]
- Worked on device logistics and reached out to possible advisors for development [Kyle]
- Procured space in WashU makerspace for device prototyping [Cam]
- Solidified wiki homepage formatting [Havisha]
- Looked into longevity of E. coli and yeast and possible alternatives to maintaining a live culture in device [Diva]
Tuesday, July 17
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- Researched biobrick scars and Gibson assembly as a possible alternative [Elizabeth]
- Finalized gene sequences for resistance genes [Elizabeth]
- Began developing poster for EECE interns presentation [Havisha]
- Ordered ribitol for testing transformed cells [Cam]
- Contacted Bayer for possible collaborations with education and human practices [Kyle]
- Worked on device design [Kyle]
- Explored powdered cells for one time use as an alternative to a live culture in device [Diva]
Wednesday, July 18
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- Finished poster for presentation [Cam, Diva, Elizabeth, Havisha, Kyle]
- Worked on summary presentation for video conference [Cam, Diva, Elizabeth, Havisha, Kyle]
- Looked into plasmids and promoter to use with ribitol operon [Havisha]
- Began overnight culture of DH5α [Havisha]
Thursday, July 19
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- ploop
Friday, July 20
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- ploop
Saturday, July 21
No work done today!
Sunday, July 22
No work done today!
Monday, July 23
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- ploop
Tuesday, July 24
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- ploop
Wednesday, July 25
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- ploop
Thursday, July 26
Present: Cam, Diva, Elizabeth, Kyle
- ploop
Friday, July 27
Present: Cam, Diva, Elizabeth, Kyle
- ploop
Saturday, July 28
Present: Cam, Diva, Elizabeth, Kyle
- ploop
Sunday, July 29
Present: Micah, Collin
Lab Work
Present: Cam, Diva, Elizabeth, Kyle
- ploop
Monday, July 30
Present: Cam, Diva, Elizabeth, Kyle
- ploop
Tuesday, July 31
Present: Cam, Diva, Elizabeth, Kyle
- ploop
Wednesday, August 1
Present: Cam, Diva, Elizabeth, Kyle
- ploop
Thursday, August 2
Present: Cam, Diva, Elizabeth, Kyle
- ploop
Friday, August 3
Present: Cam, Diva, Elizabeth, Kyle
- ploop
Saturday, August 4
Present: Cam, Diva, Elizabeth, Kyle
- ploop
Sunday, August 5
Present: Cam, Diva, Elizabeth, Kyle
- ploop
Monday, August 6
Present: Cam, Diva, Elizabeth, Kyle
- ploop
Tuesday, August 7
Present: Cam, Diva, Elizabeth, Kyle
- ploop
Wednesday, August 8
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- ploop
Thursday, August 9
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- ploop
Friday, August 10
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- ploop
Saturday, August 11
Present: Cam, Diva, Elizabeth, Havisha, Kyle
- ploop
Saturday, August 12
Present: Mark, Micah, Collin, Alex
Lab Work
Outside Work / Discussion
- Met up with the Missouri S&T iGEM Team in Rolla Missouri [Alex, Collin, Mark, Micah]
Sunday, August 13
Present: Maddie, Alex
Lab Work
- Gel purified lac+RBS+pSB1C3,lac+RBS+phrAC+pSB1C3, and lac+RBS+uvsE+pSB1C3 [Maddie]
- Ligated lac+RBS+uvsE and lac+RBS+phrAC [Alex]
- Transformed lac+RBS+uvsE and lac+RBS+phrAC into DH5α E. Coli cells [Alex]
Outside Work / Discussion
Monday, August 14
Present: Mark, Micah, Maddie, Alex
Lab Work
- Digested phrAT+pSB1C3 [Maddie, Mark]
- Prepared liquid cultures of lac+RBS+uvsE+pSB1C3 and lac+RBS+phrAC+pSB1C3 (3 cultures of each; #20, #21, #22) [Alex, Mark]
- Performed the UV irradiation test on DH5α cells with Dsup (with time intervals of 0, 1, 2, 3, and 4 hours) [Micah, Mark, Maddie]
- Performed PCR to add Golden Braid ends to Dsup [Alex]
Outside Work / Discussion
Tuesday, August 15
Present: Mark, Micah, Maddie, Alex
Lab Work
- Counted the colonies on the plates from the previous day's UV irradiation test [Micah, Mark, Alex]
- Miniprepped the overnight cultures of lac+RBS+uvsE+pSB1C3 and lac+RBS+phrAC+pSB1C3 [Maddie]
- Digested phrAT+pSB1C3, lac+RBS+phrAC+pSB1C3, and lac+RBS+uvsE [Mark]
- Performed gel electrophoresis on the digested phrAT+pSB1C3, lac+RBS+phrAC+pSB1C3, and lac+RBS+uvsE [Mark]
- Prepared liquid cultures of induced lac+RBS+Dsup+RBS+Blue chromoprotein+pSB1C3, non-induced lac+RBS+Dsup+RBS+Blue chromoprotein+pSB1C3, control DH5α E. Coli cells and lac+RBS+uvsE [Alex]
- Performed PCR to add golden gate compatible ends to phrAT [Maddie]
- Prepared LB+CM mini plates [Mark]
Outside Work / Discussion
Wednesday, August 16
Present: Zoe, Mark, Micah, Maddie, Alex
Lab Work
- Performed gel purification on phrAT+pSB1C3 [Maddie]
- Prepared 1200 mL of LB+Agar [Maddie]
- Performed the UV irradiation test on Dsup and the control [Micah, Mark]
- Performed restriction digestion on lac+RBS+uvsE+pSB1C3, lac+RBS+phrAC+pSB1C3, and lac+RBS+pSB1C3 [Maddie, Mark]
- Performed gel electrophoresis on the digested DNA [Mark]
- Ligated lac+RBS+uvSE+pSB1C3 with lac+RBS+blue chromoprotein, lac+RBS+pSB1C3 with blue chromoprotein, and lac+RBS+pSB1C3 with phrAT [Zoe, Alex]
Outside Work / Discussion
Thursday, August 17
Present: Zoe, Mark, Micah, Maddie, Alex
Lab Work
- Digested lac+RBS+phrAC+pSB1C3 [Maddie]
- Counted the colonies on the plates from the previous day's UV irradiation test [Micah, Mark, Alex, Zoe]
- Performed a PCR gradient of uvsE for GoldenBraid [Maddie]
- Prepared overnight cultures of Dsup+BCP, lac+RBS+phrAT+pSB1C3, and RBS+BCP+pSB1C3 [Mark]
Outside Work / Discussion
Friday, August 18
Present: Zoe, Mark, Micah, Maddie, Alex
Lab Work
- Prepared glycerol stocks of DH5α E. coli cells that contained lac+RBS+phrAT+pSB1C3, lac+RBS+BCP+pSB1C3, and lac+RBS+Dsup+RBS+BCP+pSB1C3 [Mark]
- Miniprepped lac+RBS+phrAT+pSB1C3 [Zoe]
- Performed a UV irradiation test for Dsup and the Blue chromoprotein control [Micah, Mark]
- Performed PCR for uvsE [Maddie, Zoe]
- Digested lac+RBS+phrAT+pSB1C3 [Alex]
- Ran a gel electrophoresis for the digested lac+RBS+phrAT+pSB1C3 [Mark]
Outside Work / Discussion
Saturday, August 19
Present: Zoe, Maddie, Mark
Lab Work
- Counted colonies on the plates from the UV irradiation test on Dsup [Zoe, Maddie, Mark]
Outside Work / Discussion
Sunday, August 20
Present: Micah
Lab Work
- Prepared overnight cultures of DH5α E. coli cells with lac+RBS+blue chromoprotein+pSB1C3, lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3, and lac+RBS+uvsE+RBS+blue chromoprotein+pSB1C3 (#1) [Micah]
Outside Work / Discussion
Monday, August 21
Present:Mark, Micah
Lab Work
- Prepared overnight cultures of DH5α E. coli cells with pSB1C3+lac+RBS+phrAC+RBS+blue chromoprotein (2 cultures, #1 and #2), pSB1C3+lac+RBS+blue chromoprotein, and pSB1C3+lac+RBS+Dsup+RBS+blue chromoprotein [Mark]
- Miniprepped DH5α E. coli cell cultures with lac+RBS+blue chromoprotein+pSB1C3, lac+RBS+Dsup+RBS+blue chromoprotein+pSB1C3, and lac+RBS+uvsE+RBS+blue chromoprotein+pSB1C3 (#1) [Micah]
Outside Work / Discussion
- Watched the Great American Eclipse of 2017 [Micah, Mark, Zoe, Maddie, Alex, Collin]
Tuesday, August 22
Present: Mark, Zoe, Micah, Alex, Maddie
Lab Work
- Miniprepped lac+RBS+phrAC+RBS+BCP+pSB1C3 [Zoe]
- Gel purified lac+RBS+phrAT+pSB1C3 [Maddie]
- Prepared LB+CM plates [Mark]
- Performed the UV irradiation test on uvsE, phrAC, and the blue chromoprotein control [Micah, Mark]
Outside Work / Discussion
Wednesday, August 23
Present: Zoe, Maddie, Mark, Alex, Micah
Lab Work
- Counted the colonies on the plates from the previous day's UV irradiation test [Micah, Alex, Mark, Zoe, Maddie]
- Streaked lac+RBS+uvsE+RBS+BCP+pSB1C3 and lac+RBS+phrAC+RBS+BCP+pSB1C3 onto LB+CM plates [Micah]
- Ligated lac+RBS+phrAT+pSB1C3 with RBS+BCP [Micah]
- Transformed DH5α E. coli cells with lac+RBS+phrAT+RBS+BCP+pSB1C3 [Mark]
Outside Work / Discussion
Thursday, August 24
Present:Micah
Lab Work
- Prepared overnight cultures of DH5α E. coli cells with lac+RBS+phrAT+RBS+BCP+pSB1C3 {Micah]
Outside Work / Discussion
Friday, August 25
Present: Zoe
Lab Work
- Miniprepped lac+RBS+phrAT+RBS+BCP+pSB1C3 [Zoe]
Outside Work / Discussion
Saturday, August 26
No work done today!
Sunday, August 27
No work done today!
Monday, August 28
No work done today!
Tuesday, August 29
No work done today!
Wednesday, August 30
No work done today!
Thursday, August 31
Present: Collin
Lab Work
- Prepared overnight cultures from glycerol stock of lac+RBS+BCP+pSB1C3 [Collin]
Outside Work / Discussion
Friday, September 1
No work done today!
Saturday, September 2
No work done today!
Sunday, September 3
No work done today!
Monday, September 4
No work done today!
Tuesday, September 5
No work done today!
Wednesday, September 6
No work done today!
Thursday, September 7
No work done today!
Friday, September 8
No work done today!
Saturday, September 9
No work done today!
Sunday, September 10
No work done today!
Monday, September 11
No work done today!
Tuesday, September 12
No work done today!
Wednesday, September 13
No work done today!
Thursday, September 14
Present:Mark
Lab Work
- Transformed Lac+RBS+BCP+pSB1C3 into E.coli cells [Mark]
Outside Work / Discussion
Friday, September 15
No work done today!
Saturday, September 16
Present:Maddie
Lab Work
- Started a cyanobacteria culture [Maddie]
- Performed GoldenBraid PCR for uvsE, phrAC, phrAT, and Dsup [Maddie]
Outside Work / Discussion
Sunday, September 17
No work done today!
Monday, September 18
No work done today!
Tuesday, September 19
No work done today!
Wednesday, September 20
No work done today!
Thursday, September 21
No work done today!
Friday, September 22
No work done today!
Saturday, September 23
No work done today!
Sunday, September 24
No work done today!
Monday, September 25
No work done today!
Tuesday, September 26
No work done today!
Wednesday, September 27
No work done today!
Thursday, September 28
Present: Collin
Lab Work
- Refreshed Cyanobacteria culture [Collin]
Outside Work / Discussion
Friday, September 29
No work done today!
Saturday, September 30
Present:Collin, Micah, Mark
Lab Work
- Performed PCR on Dsup, uvsE, phrAC, and phrAT for the GoldenBraid assembly [Collin]
- Performed the UV irradiaton test for uvsE, phrAC, and the control [Micah, Mark, Collin]
Outside Work / Discussion
Sunday, October 1
No work done today!
Monday, October 2
Present: Collin
Lab Work
- Measured the ODs of the Cyanobacteria culture [Collin]
- 3.413 (#1), 0.1370 (#2)
Outside Work / Discussion
Tuesday, October 3
Present:Maddie
Lab Work
- Measured the OD of the cyanobacteria culture [Maddie]
- 0.1452 (#2)
Outside Work / Discussion
Wednesday, October 4
Present:Maddie
Lab Work
- Measured the OD of the cyanobacteria culture [Maddie]
- 0.3725 (#2)
Outside Work / Discussion
Thursday, October 5
Present:Maddie
Lab Work
- Measured the OD of the cyanobacteria culture [Maddie]
- 0.6746 (#2)
Outside Work / Discussion
Friday, October 6
Present:Mark
Lab Work
- Prepared LB Media [Mark]
Outside Work / Discussion
Saturday, October 7
Present:Mark, Maddie
Lab Work
- Prepared LB Agar [Mark]
- Prepared overnight cultures of BCP, uvsE, and phrAC [Maddie]
Outside Work / Discussion
Sunday, October 8
Present:Mark, Micah
Lab Work
- Prepared for a UV irradiation test for uvsE, phrAC, and BCP control [Mark, Micah]
Outside Work / Discussion
Monday, October 9
Present: Collin
Lab Work
- Measured the OD of the cyanobacteria culture [Maddie]
- 3.1760 (#2)
Outside Work / Discussion
Tuesday, October 10
Present: Collin
Lab Work
- Digested neomycin phosphotransferase (nptII) and pSB1C3 [Colin]
- Ran a gel electrophoresis for the digested nptII and pSB1C3 [Collin]
- Transformed uvsE+BCP into E. coli [Collin]
Outside Work / Discussion
Wednesday, October 11
Present: Collin
Lab Work
- Made Kanamycin plates [Collin]
- Prepared bacterial stabs of Dsup, phrAC, and phrAT for the UChicago team [Collin]
Outside Work / Discussion
Thursday, October 12
No work done today!
Friday, October 13
No work done today!
Saturday, October 14
No work done today!
Sunday, October 15
No work done today!
Monday, October 16
Present: Mark
Lab Work
- Gel purified pSB1C3 and nptII [Mark]
- Ligated pSB1C3 and nptII [Mark]
- Transformed pSB1C3+nptII [Mark]
Outside Work / Discussion
Tuesday, October 17
No work done today!
Wednesday, October 18
No work done today!
Thursday, October 19
Present: Collin
Lab Work
- Streaked phrAT, phrAC, BCP, uvsE, and BCP [Collin]
Outside Work / Discussion
Friday, October 20
Present: Collin
Lab Work
- Transformed lac+RBS+Dsup+BCP into E.coli cells [Collin]
- Prepared cultures of lac+RBS+Dsup, lac+RBS+BCP, and lac+RBS+uvsE [Collin]
Outside Work / Discussion
Saturday, October 21
Present: Micah
Lab Work
- Transformed medium promoter+medium RBS (BBa_K608006) into E. coli [Micah]
- Prepared cultures from previous transformation [Micah]
Outside Work / Discussion
Sunday, October 22
Present:Collin
Lab Work
- Made glycerol stocks of lac+RBS+Dsup+RBS+BCP+pSB1C3 [Collin]
Outside Work / Discussion
Monday, October 23
Present: Collin
Lab Work
- Miniprepped lac+RBS+BCP+pSB1C3 [Collin]
Outside Work / Discussion
Wednesday, October 24
Giant Jamboree!
Thursday, October 25
Giant Jamboree!
Friday, October 26
Giant Jamboree!
Saturday, October 27
Giant Jamboree!
Sunday, October 28
Giant Jamboree!
Monday, October 29
No work done today!
Tuesday, October 30
No work done today!
Wednesday, October 31
No work done today!