Difference between revisions of "Team:Queens Canada/Safety"

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<h1> Safety </h1>
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<h1>Safety</h1>
<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>By ensuring all of our QGEM team members have undergone the necessary laboratory safety training, Workplace Hazardous Materials Information System (WHMIS) training and Biosafety Level 1 training, we are creating a safe and informed workplace.</p>
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<p>Prior to any experiments, the required SDS are read to ensure all relevant safety information, personal protective equipment (PPE) is worn and all necessary precautions are taken to avoid any harm to lab workers and the environment.  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where <em>you</em> can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<p>Lab safety training includes the following:</p>
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<li>Location and use of fire alarms, exits, and extinguishers</li>
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<li>Location and use of eyewash stations, safety showers, first aid kits, chemical spill kits and response cards, laboratory gas shut off valves</li>
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<li>Standard laboratory safety rules, including proper lab wear, protective gear, use of hazardous equipment</li>
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<li>Proper method of disposal and spill response for biohazardous materials, sharps, chemicals, radioactive material, ethidium bromide gels</li>
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<li>Proper use and handling of autoclaves (undergone with the department's designated trainer)</li>
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<p>Risk Group 1 (non-pathogenic organisms) Escherichia coli DH5-a strain in regulation with the Canadian Biosafety Standards was used for plasmid housing, has no 'natural' environment or habitat as it does not exist in nature. The primary strain of E. coli used for expression was BL21 and it does not pose any threat to laboratory workers, the general public, or the environment. Standard lab biosafety regulations were followed to ensure that any modified strains were not introduced to people or the environment outside of the lab.</p>
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<p>All of our work is done under the direct supervision of Dr. Martin Petkovich, Dr. Donald Cameron, Dr. Virginia Walker or Kristy Moniz to ensure lab equipment and materials are being utilized in a safe and effective manner.</p>
 
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Revision as of 20:05, 18 July 2018

Safety

By ensuring all of our QGEM team members have undergone the necessary laboratory safety training, Workplace Hazardous Materials Information System (WHMIS) training and Biosafety Level 1 training, we are creating a safe and informed workplace.

Prior to any experiments, the required SDS are read to ensure all relevant safety information, personal protective equipment (PPE) is worn and all necessary precautions are taken to avoid any harm to lab workers and the environment.

Lab safety training includes the following:

  • Location and use of fire alarms, exits, and extinguishers
  • Location and use of eyewash stations, safety showers, first aid kits, chemical spill kits and response cards, laboratory gas shut off valves
  • Standard laboratory safety rules, including proper lab wear, protective gear, use of hazardous equipment
  • Proper method of disposal and spill response for biohazardous materials, sharps, chemicals, radioactive material, ethidium bromide gels
  • Proper use and handling of autoclaves (undergone with the department's designated trainer)

Risk Group 1 (non-pathogenic organisms) Escherichia coli DH5-a strain in regulation with the Canadian Biosafety Standards was used for plasmid housing, has no 'natural' environment or habitat as it does not exist in nature. The primary strain of E. coli used for expression was BL21 and it does not pose any threat to laboratory workers, the general public, or the environment. Standard lab biosafety regulations were followed to ensure that any modified strains were not introduced to people or the environment outside of the lab.

All of our work is done under the direct supervision of Dr. Martin Petkovich, Dr. Donald Cameron, Dr. Virginia Walker or Kristy Moniz to ensure lab equipment and materials are being utilized in a safe and effective manner.

Safe Project Design

Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:

  • Choosing a non-pathogenic chassis
  • Choosing parts that will not harm humans / animals / plants
  • Substituting safer materials for dangerous materials in a proof-of-concept experiment
  • Including an "induced lethality" or "kill-switch" device

Safe Lab Work

What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!

Safe Shipment

Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?

These are some of our safety pictures

Lab bench

Our main lab bench

This is our lab bench where we work safely


Our emergency shower

Eshower

Espill kit

Our emergency spill kit