Description

With the growth of synthetic biology, there has been an increase in the development of digital synthetic circuits, which requires biological logic gates that can accept a binary input and generate a suitable binary output. But biological systems are often unable to provide sharp and accurate input to output response due to various reasons such as noise, growth factors etc. Hence there exists a need of robust and reliable modules that can transform the analog and stochastic behaviours of biology into a digital response. Our recombinase based elementary constructs would allow development of such complex circuits with specialized functions with greater ease.

Recombinases are enzymes that have proven to be highly robust and efficient. They trigger site-specific recombination to perform excision/incision or inversion of genetic circuits, exclusively or in tandem to produce the desired gene expression. Our project involves use of serine based recombinases to develop a novel recombinase based toolkit of elementary circuits such as switch, feedforward loop, feedback loop etc. This toolkit will serve as the vanguard in development of modular and orthogonal systems that can be tuned provide the desired output.