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Revision as of 12:55, 3 September 2018
Alternative Roots
Attributions and
Acknowledgements
2018 Newcastle iGEM
Attributions
Initial Project Idea
- Lewis Tomlinson
- Connor Trotter
- Chris Carty
- Frank Eardley
- Heather Bottomley
- Luke Waller
- Patrycja Ubysz
- Umar Farooq
- Will Tankard
- Sadiya Quazi
Project Support
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vestibulum accumsan, urna a semper fringilla, magna libero pretium turpis, at efficitur leo diam nec mi. Donec rhoncus varius magna, a pulvinar nibh convallis sed. Praesent in dignissim lorem. Integer dignissim mi id auctor blandit. Vivamus eget justo vehicula, pulvinar nibh quis, imperdiet neque. Mauris nunc lorem, sodales non sapien in, pellentesque fringilla libero. Suspendisse dignissim, turpis non interdum tempor, urna ante tempus leo, vel posuere ante nunc ut magna. Phasellus vel tellus eros. Nulla pellentesque porttitor nibh eu gravida. Curabitur faucibus ante rhoncus, rhoncus elit sit amet, sollicitudin lorem.
Will Tankard
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vestibulum accumsan, urna a semper fringilla, magna libero pretium turpis, at efficitur leo diam nec mi. Donec rhoncus varius magna, a pulvinar nibh convallis sed. Praesent in dignissim lorem. Integer dignissim mi id auctor blandit. Vivamus eget justo vehicula, pulvinar nibh quis, imperdiet neque. Mauris nunc lorem, sodales non sapien in, pellentesque fringilla libero. Suspendisse dignissim, turpis non interdum tempor, urna ante tempus leo, vel posuere ante nunc ut magna. Phasellus vel tellus eros. Nulla pellentesque porttitor nibh eu gravida. Curabitur faucibus ante rhoncus, rhoncus elit sit amet, sollicitudin lorem.
Heather Bottomley
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vestibulum accumsan, urna a semper fringilla, magna libero pretium turpis, at efficitur leo diam nec mi. Donec rhoncus varius magna, a pulvinar nibh convallis sed. Praesent in dignissim lorem. Integer dignissim mi id auctor blandit. Vivamus eget justo vehicula, pulvinar nibh quis, imperdiet neque. Mauris nunc lorem, sodales non sapien in, pellentesque fringilla libero. Suspendisse dignissim, turpis non interdum tempor, urna ante tempus leo, vel posuere ante nunc ut magna. Phasellus vel tellus eros. Nulla pellentesque porttitor nibh eu gravida. Curabitur faucibus ante rhoncus, rhoncus elit sit amet, sollicitudin lorem.
Connor Trotter
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vestibulum accumsan, urna a semper fringilla, magna libero pretium turpis, at efficitur leo diam nec mi. Donec rhoncus varius magna, a pulvinar nibh convallis sed. Praesent in dignissim lorem. Integer dignissim mi id auctor blandit. Vivamus eget justo vehicula, pulvinar nibh quis, imperdiet neque. Mauris nunc lorem, sodales non sapien in, pellentesque fringilla libero. Suspendisse dignissim, turpis non interdum tempor, urna ante tempus leo, vel posuere ante nunc ut magna. Phasellus vel tellus eros. Nulla pellentesque porttitor nibh eu gravida. Curabitur faucibus ante rhoncus, rhoncus elit sit amet, sollicitudin lorem.
Patrycja Ubysz
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vestibulum accumsan, urna a semper fringilla, magna libero pretium turpis, at efficitur leo diam nec mi. Donec rhoncus varius magna, a pulvinar nibh convallis sed. Praesent in dignissim lorem. Integer dignissim mi id auctor blandit. Vivamus eget justo vehicula, pulvinar nibh quis, imperdiet neque. Mauris nunc lorem, sodales non sapien in, pellentesque fringilla libero. Suspendisse dignissim, turpis non interdum tempor, urna ante tempus leo, vel posuere ante nunc ut magna. Phasellus vel tellus eros. Nulla pellentesque porttitor nibh eu gravida. Curabitur faucibus ante rhoncus, rhoncus elit sit amet, sollicitudin lorem.
Lewis Tomlinson
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vestibulum accumsan, urna a semper fringilla, magna libero pretium turpis, at efficitur leo diam nec mi. Donec rhoncus varius magna, a pulvinar nibh convallis sed. Praesent in dignissim lorem. Integer dignissim mi id auctor blandit. Vivamus eget justo vehicula, pulvinar nibh quis, imperdiet neque. Mauris nunc lorem, sodales non sapien in, pellentesque fringilla libero. Suspendisse dignissim, turpis non interdum tempor, urna ante tempus leo, vel posuere ante nunc ut magna. Phasellus vel tellus eros. Nulla pellentesque porttitor nibh eu gravida. Curabitur faucibus ante rhoncus, rhoncus elit sit amet, sollicitudin lorem.
Sadiya Quazi
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vestibulum accumsan, urna a semper fringilla, magna libero pretium turpis, at efficitur leo diam nec mi. Donec rhoncus varius magna, a pulvinar nibh convallis sed. Praesent in dignissim lorem. Integer dignissim mi id auctor blandit. Vivamus eget justo vehicula, pulvinar nibh quis, imperdiet neque. Mauris nunc lorem, sodales non sapien in, pellentesque fringilla libero. Suspendisse dignissim, turpis non interdum tempor, urna ante tempus leo, vel posuere ante nunc ut magna. Phasellus vel tellus eros. Nulla pellentesque porttitor nibh eu gravida. Curabitur faucibus ante rhoncus, rhoncus elit sit amet, sollicitudin lorem.
Umar Farooq
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vestibulum accumsan, urna a semper fringilla, magna libero pretium turpis, at efficitur leo diam nec mi. Donec rhoncus varius magna, a pulvinar nibh convallis sed. Praesent in dignissim lorem. Integer dignissim mi id auctor blandit. Vivamus eget justo vehicula, pulvinar nibh quis, imperdiet neque. Mauris nunc lorem, sodales non sapien in, pellentesque fringilla libero. Suspendisse dignissim, turpis non interdum tempor, urna ante tempus leo, vel posuere ante nunc ut magna. Phasellus vel tellus eros. Nulla pellentesque porttitor nibh eu gravida. Curabitur faucibus ante rhoncus, rhoncus elit sit amet, sollicitudin lorem.
Chris Carty
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vestibulum accumsan, urna a semper fringilla, magna libero pretium turpis, at efficitur leo diam nec mi. Donec rhoncus varius magna, a pulvinar nibh convallis sed. Praesent in dignissim lorem. Integer dignissim mi id auctor blandit. Vivamus eget justo vehicula, pulvinar nibh quis, imperdiet neque. Mauris nunc lorem, sodales non sapien in, pellentesque fringilla libero. Suspendisse dignissim, turpis non interdum tempor, urna ante tempus leo, vel posuere ante nunc ut magna. Phasellus vel tellus eros. Nulla pellentesque porttitor nibh eu gravida. Curabitur faucibus ante rhoncus, rhoncus elit sit amet, sollicitudin lorem.
Parts
Check out what parts we're using
We will be utilising iGEM registry parts sequenced by IDT to construct a biosynthetic operon for expression in Escherichia coli. The operon will synthesise naringenin, a flavonoid that attracts nitrogen fixing bacteria. The operon will contain the genes for four enzymes: 4 – Coumaryl ligase – 4CL (BBa_K1033001), Tyrosine ammonia lyase - TAL (BBa_K1033000), Chalcone isomerase - CHI (BBa_K1497000) and Chalcone synthase - CHS (BBa_K1497001). Each of these parts contain a strong ribosome binding site (BBa_B0034). This construct will be under the control of a T7 promoter (BBa_I712074) to observe its expression in E. coli as a proof of concept. Once biosynthesis under the control of T7 is achieved, the construct will be tested under a constitutive promoter (BBa_J23100). Parts for biosynthesis in the final chassis organism, root-colonising Pseudomonas fluorescens, will be constructed in the plasmid backbone pBSC1C3.