ElishaKrauss (Talk | contribs) |
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− | <h3 style="width:70%;margin-left:15%"> | + | <h3 style="width:70%;margin-left:15%">Michaelis - Menten kinetics</h3> |
<p style="width:70%;margin-left:15%">Michaelis - Menten kinetics is a model used to examine enzyme kinetic. Luciferase's activity can be modeled by Michaelis-Menten kinetics as they perform the simple conversion of a substrate into a product and a photon. Our project relied on the light producing NanoLuc Luciferase as a signal in our devices. We were able to model this relationship with MATLAB. The governing equations | <p style="width:70%;margin-left:15%">Michaelis - Menten kinetics is a model used to examine enzyme kinetic. Luciferase's activity can be modeled by Michaelis-Menten kinetics as they perform the simple conversion of a substrate into a product and a photon. Our project relied on the light producing NanoLuc Luciferase as a signal in our devices. We were able to model this relationship with MATLAB. The governing equations | ||
for this model were compiled in the MATLAB, with the goal of creating a generic calculator which teams may use in the future. Known | for this model were compiled in the MATLAB, with the goal of creating a generic calculator which teams may use in the future. Known | ||
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+ | <h3 style="width:70%;margin-left:15%">Fluid Dynamics</h3> | ||
+ | <p style="width:70%;margin-left:15%">The ultimate application of our work from this year will be in the form of a diagnostic pacifier capable of collecting saliva, mixing with an internal biosensor and generating a signal for salivary hormone quantification. Therefore we sought to model many aspects of the pacifier including: saliva flow rate, flow turbulence, and particle mixing. | ||
+ | |||
+ | <img src="https://static.igem.org/mediawiki/2018/0/06/T--Queens_Canada--BrownianSimulation2FD.png" alt='Diagram showing Brownian simulations in a tube' /> | ||
+ | <figcaption>Figure 4: Simulations of Brownian motion within a cylinder</figcaption> | ||
Revision as of 02:17, 11 October 2018
At team Queens Canada, we believe that proper preparation is the best way to reach a desired outcome. Accordingly, we sought to model many aspects of our project which aided in making the right choices in the lab and receiving positive results. Through the help of student on our team specializing in biomedical computing, applied mathematics, and chemical engineering, we created a number of different models that were crucial to our project design.
Molecular Dynamic Simulations
One of our constructs relied on linkers of sufficient length and flexibility to convert a conformational change, into signal transduction. We have achieved this through firstly modeling with PyMol [[[[LINK TO LINKER DEVELOPMENT TAB]]] and then performing molecular dynamic simulations of the root-mean-square deviation of atomic position [[[LINK TO Molecular Dynamics Tab]]]].