Difference between revisions of "Team:Tongji China/T3SS"
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Type III secretion system <br><br>(T3SS) | Type III secretion system <br><br>(T3SS) | ||
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Type III secretion system (T3SS) is a highly coordinated multi-protein system which consists of structural, regulatory and secreted proteins. The structure of the type III secretion nanomachine (or injectisome) is highly conserved among Gram-negative bacteria. Due to making good use of its infecting potential, T3SS can be an amazing tool to deliver proteins that we can reestablish a system of our protein genes of interest.<br> | Type III secretion system (T3SS) is a highly coordinated multi-protein system which consists of structural, regulatory and secreted proteins. The structure of the type III secretion nanomachine (or injectisome) is highly conserved among Gram-negative bacteria. Due to making good use of its infecting potential, T3SS can be an amazing tool to deliver proteins that we can reestablish a system of our protein genes of interest.<br> | ||
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Structure - injectisome | Structure - injectisome | ||
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The needle complex is composed of a multi-ring cylindrical base with ~26 nm in diameter that is anchored on the bacterial envelope and a needle-like structure that projects ~60 nm from the bacterial surface. The entire structure is traversed by a channel ~2 nm in diameter that serves as a conduit for the passage of proteins injected through the type III secretion machinery. Protein export through the injectisome is fueled by an ATPase at the cytoplasmic sorting platform.<br><br><br> | The needle complex is composed of a multi-ring cylindrical base with ~26 nm in diameter that is anchored on the bacterial envelope and a needle-like structure that projects ~60 nm from the bacterial surface. The entire structure is traversed by a channel ~2 nm in diameter that serves as a conduit for the passage of proteins injected through the type III secretion machinery. Protein export through the injectisome is fueled by an ATPase at the cytoplasmic sorting platform.<br><br><br> | ||
<p style="text-align:center"><img src="https://static.igem.org/mediawiki/2018/c/c3/T--Tongji_China--picture-Project-Background--t3ss-1_structure.png" width="40%" height="40%" ></p> | <p style="text-align:center"><img src="https://static.igem.org/mediawiki/2018/c/c3/T--Tongji_China--picture-Project-Background--t3ss-1_structure.png" width="40%" height="40%" ></p> | ||
| − | <div class="instructionOfPicture" | + | <div class="instructionOfPicture">Figure1. Injectisome structure</div> |
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Process - delivery<br><br> | Process - delivery<br><br> | ||
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First T3SS comes into being by attaching with host cells. A set of pore-forming proteins are transported through the needle and are inserted into the eukaryotic cell membrane to form a pore of approximately 3–6 nm in diameter, called translocon. Following the pore formation, type III secretion regulatory protein (repressor) is secreted, resulting in transcriptional activation of the whole T3SS regulon genes. The above is called polar-translocation.<br> | First T3SS comes into being by attaching with host cells. A set of pore-forming proteins are transported through the needle and are inserted into the eukaryotic cell membrane to form a pore of approximately 3–6 nm in diameter, called translocon. Following the pore formation, type III secretion regulatory protein (repressor) is secreted, resulting in transcriptional activation of the whole T3SS regulon genes. The above is called polar-translocation.<br> | ||
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| − | Then viral and bacterial epitopes, as well as peptides from human tumors, have been translated into protein and delivered by the bacterial T3SS with the aim to elicit immune response (vaccination) or cancer immunotherapy.<br><br><br>< | + | Then viral and bacterial epitopes, as well as peptides from human tumors, have been translated into protein and delivered by the bacterial T3SS with the aim to elicit immune response (vaccination) or cancer immunotherapy.<br><br><br> |
| − | < | + | <div class="reference"><b>References:</b><br> |
| − | [2] | + | [1] Bai F, Li Z, Umezawa A et al. Bacterial type III secretion system as a protein delivery tool for a broad range of biomedical applications. Biotechnol Adv 2018;36:482–93. <br> |
| + | [2] Galle M, Carpentier I, Beyaert R. Structure and function of the Type III secretion system of Pseudomonas aeruginosa. Curr Protein Pept Sci. 2012;13:831–42. doi: 10.2174/138920312804871210.<br><br></div> | ||
<a href="https://2018.igem.org/Team:Tongji_China/Background"><font size=3>Return to the Background Overview</font></a><br> | <a href="https://2018.igem.org/Team:Tongji_China/Background"><font size=3>Return to the Background Overview</font></a><br> | ||
<a href="https://2018.igem.org/Team:Tongji_China/Neoantigen"><font size=3>Go to the Background Neoantigen</font></a><br> | <a href="https://2018.igem.org/Team:Tongji_China/Neoantigen"><font size=3>Go to the Background Neoantigen</font></a><br> | ||
Revision as of 12:17, 13 October 2018
Background
Type III secretion system
(T3SS)
(T3SS)
Type III secretion system (T3SS) is a highly coordinated multi-protein system which consists of structural, regulatory and secreted proteins. The structure of the type III secretion nanomachine (or injectisome) is highly conserved among Gram-negative bacteria. Due to making good use of its infecting potential, T3SS can be an amazing tool to deliver proteins that we can reestablish a system of our protein genes of interest.
Structure - injectisome
The T3SS injectisome is composed of a needle complex, an inner membrane export apparatus, and a cytoplasmic platform that energizes the secretion process and selectively sorts substrates for their orderly delivery to the secretion machine.
The needle complex is composed of a multi-ring cylindrical base with ~26 nm in diameter that is anchored on the bacterial envelope and a needle-like structure that projects ~60 nm from the bacterial surface. The entire structure is traversed by a channel ~2 nm in diameter that serves as a conduit for the passage of proteins injected through the type III secretion machinery. Protein export through the injectisome is fueled by an ATPase at the cytoplasmic sorting platform.
Figure1. Injectisome structure
Process - delivery
First T3SS comes into being by attaching with host cells. A set of pore-forming proteins are transported through the needle and are inserted into the eukaryotic cell membrane to form a pore of approximately 3–6 nm in diameter, called translocon. Following the pore formation, type III secretion regulatory protein (repressor) is secreted, resulting in transcriptional activation of the whole T3SS regulon genes. The above is called polar-translocation.
Then viral and bacterial epitopes, as well as peptides from human tumors, have been translated into protein and delivered by the bacterial T3SS with the aim to elicit immune response (vaccination) or cancer immunotherapy.
References:
[1] Bai F, Li Z, Umezawa A et al. Bacterial type III secretion system as a protein delivery tool for a broad range of biomedical applications. Biotechnol Adv 2018;36:482–93.
[2] Galle M, Carpentier I, Beyaert R. Structure and function of the Type III secretion system of Pseudomonas aeruginosa. Curr Protein Pept Sci. 2012;13:831–42. doi: 10.2174/138920312804871210.
Return to the Background Overview[1] Bai F, Li Z, Umezawa A et al. Bacterial type III secretion system as a protein delivery tool for a broad range of biomedical applications. Biotechnol Adv 2018;36:482–93.
[2] Galle M, Carpentier I, Beyaert R. Structure and function of the Type III secretion system of Pseudomonas aeruginosa. Curr Protein Pept Sci. 2012;13:831–42. doi: 10.2174/138920312804871210.
Go to the Background Neoantigen
Go to the Background P.A.