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                     <li>We have identified an origin or replication and two selectable antibiotic markers (gentamicin and streptomycin) that can be used with our Pseudomonas sp.</li>
 
                     <li>We have identified an origin or replication and two selectable antibiotic markers (gentamicin and streptomycin) that can be used with our Pseudomonas sp.</li>
  
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                            <h3 class="subhead">Microbial Community Engineering</h3>
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                    <li>We have characterised the responses of three free-living, nitrogen fixing bacteria to the chemoattractant naringenin and demonstrated that two are attracted to this flavonoid.</li>
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                    <li>In tandem with our experimental characterisation we have built an Agent Based Model that indicates that naringenin biosynthesis by a plant endophyte would result in the formation of a biofilm by N2-fixing bacteria.</li>
 
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Revision as of 10:28, 12 October 2018

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Alternative Roots

Alternative Roots

Demonstrate

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Alternative Roots

What worked - and didn’t work - in 2018 for Alternative Roots

Overview

Alternative roots is a mulit-component project proposing that plant endophytes - microbes that live harmlessly within plant tissues - can be engineered to enhance beneficial plant : microbial interactions. This may be achieved, for example, by engineering plant endophytes to synthesise chemoattractants of free-living, nitrogen fixing bacteria. We examined both the feasibility of the biology and considered how this technology might be viably deployed in our local community. Here we highlight some of the conclusions from our work:

Chassis Development

  1. We have demonstrated colonisation of plant tissues by our Pseudomonas sp.
  2. We have demonstrated that this species is genetically transformable.
  3. We have demonstrated that transformed our Pseudomonas sp. can colonise root tissues.
  4. We have identified an origin or replication and two selectable antibiotic markers (gentamicin and streptomycin) that can be used with our Pseudomonas sp.

Microbial Community Engineering

  1. We have characterised the responses of three free-living, nitrogen fixing bacteria to the chemoattractant naringenin and demonstrated that two are attracted to this flavonoid.
  2. In tandem with our experimental characterisation we have built an Agent Based Model that indicates that naringenin biosynthesis by a plant endophyte would result in the formation of a biofilm by N2-fixing bacteria.