Difference between revisions of "Team:UAlberta/Demonstrate"

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<h1>Demonstrate</h1>
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      <div class="container">
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<h3>Gold Medal Criterion #4</h3>
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        <h1>Overview</h1>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve their gold medals!
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            <h6>Press Me</h6>
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            <i class="fas fa-arrow-down"></i>
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<p>
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          </div>
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Please see the <a href="https://2018.igem.org/Judging/Medals">2018 Medals Page</a> for more information.
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            <div class="card card-body">
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        <p>To make biology easier to engineer, standardization and other engineering principles have been adapted for and applied
 +
        to biological parts. Though, standardization must not only be implemented to parts themselves but also in standardizing
 +
        the methods we use to test these parts so that direct comparisons of designs are possible.</p>
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        <div class="row">
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          <div class="col-lg-7">
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            <p>Thus, the goal of iGEM InterLab studies is to develop standards and protocols for measuring the fluorescence response
 +
            of fluorescent proteins, a quantity commonly used in evaluating the performance of biological systems. Fluorescent
 +
            proteins, such as Green Fluorescent Protein (GFP), have become widespread tools as their fluorescence readout can be
 +
            linked with parameters like gene expression and protein interactions. Although, the lack of a widely accepted standard
 +
            protocol and variance in measurement instruments, like microplate readers, contributes to the difficulty in comparing
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            fluorescence measurements between instruments and different instances of measurement.</p>
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          </div>
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          <div class="col-lg-5 align-self-center">
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            <figure class="figure">
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              <img src="https://static.igem.org/mediawiki/2018/6/68/T--UAlberta--HoveApiaries.png" class="figure-img img-fluid rounded" alt="...">
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              <figcaption class="figure-caption text-right">A caption for the above image.</figcaption>
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            </figure>
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          </div>
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        </div>
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        <p>Thus, the goal of iGEM InterLab studies is to develop standards and protocols for measuring the fluorescence response of fluorescent proteins, a quantity commonly used in evaluating the performance of biological systems. Fluorescent proteins, such as Green Fluorescent Protein (GFP), have become widespread tools as their fluorescence readout can be linked with parameters like gene expression and protein interactions. Although, the lack of a widely accepted standard protocol and variance in measurement instruments, like microplate readers, contributes to the difficulty in comparing fluorescence measurements between instruments and different instances of measurement.</p>
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        <div class="row">
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          <div class="col-lg-6 align-self-center">
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            <figure class="figure">
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              <img src="https://static.igem.org/mediawiki/2018/6/68/T--UAlberta--HoveApiaries.png" class="figure-img img-fluid rounded" alt="...">
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              <figcaption class="figure-caption">A caption for the above image.</figcaption>
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            </figure>
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          </div>
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          <div class="col-lg-6">
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            <p>Thus, the goal of iGEM InterLab studies is to develop standards and protocols for measuring the fluorescence response
 +
            of fluorescent proteins, a quantity commonly used in evaluating the performance of biological systems. Fluorescent
 +
            proteins, such as Green Fluorescent Protein (GFP), have become widespread tools as their fluorescence readout can be
 +
            linked with parameters like gene expression and protein interactions. Although, the lack of a widely accepted standard
 +
            protocol and variance in measurement instruments, like microplate readers, contributes to the difficulty in comparing
 +
            fluorescence measurements between instruments and different instances of measurement.</p>
 +
          </div>
 +
        </div>
 +
        <p>For the 2018 InterLab Study, the variability in fluorescence measurements when assessing a population of cells was
 +
        investigated and the utility of normalizing measurements to absolute cell count or colony forming units (CFU) was
 +
        assessed <a href="">[1]</a>. Team UAlberta accomplished these objectives this by following the InterLab Study protocols to measure the
 +
        fluorescence and cellular density of eight devices which were calibrated against established standards.</p>
 +
        <h2>Materials and Methods</h2>
 +
        <p>The calibration and measurement procedures were performed by the members of Team UAlberta as outlined in the 2018
 +
        InterLab Study Protocols. To view the 2018 InterLab Study Protocols, click <a href="">here</a>. The following protocols describe Team
 +
        UAlberta’s methods when none was specified by the InterLab protocols and to provide information about the measurement
 +
        instruments.</p>
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      </div>
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    </div>
  
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      <div class="ua-footer">
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        <div class="container">
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Revision as of 02:16, 18 October 2018

...

Overview

Anim pariatur cliche reprehenderit, enim eiusmod high life accusamus terry richardson ad squid. Nihil anim keffiyeh helvetica, craft beer labore wes anderson cred nesciunt sapiente ea proident.

To make biology easier to engineer, standardization and other engineering principles have been adapted for and applied to biological parts. Though, standardization must not only be implemented to parts themselves but also in standardizing the methods we use to test these parts so that direct comparisons of designs are possible.

Thus, the goal of iGEM InterLab studies is to develop standards and protocols for measuring the fluorescence response of fluorescent proteins, a quantity commonly used in evaluating the performance of biological systems. Fluorescent proteins, such as Green Fluorescent Protein (GFP), have become widespread tools as their fluorescence readout can be linked with parameters like gene expression and protein interactions. Although, the lack of a widely accepted standard protocol and variance in measurement instruments, like microplate readers, contributes to the difficulty in comparing fluorescence measurements between instruments and different instances of measurement.

...
A caption for the above image.

Thus, the goal of iGEM InterLab studies is to develop standards and protocols for measuring the fluorescence response of fluorescent proteins, a quantity commonly used in evaluating the performance of biological systems. Fluorescent proteins, such as Green Fluorescent Protein (GFP), have become widespread tools as their fluorescence readout can be linked with parameters like gene expression and protein interactions. Although, the lack of a widely accepted standard protocol and variance in measurement instruments, like microplate readers, contributes to the difficulty in comparing fluorescence measurements between instruments and different instances of measurement.

...
A caption for the above image.

Thus, the goal of iGEM InterLab studies is to develop standards and protocols for measuring the fluorescence response of fluorescent proteins, a quantity commonly used in evaluating the performance of biological systems. Fluorescent proteins, such as Green Fluorescent Protein (GFP), have become widespread tools as their fluorescence readout can be linked with parameters like gene expression and protein interactions. Although, the lack of a widely accepted standard protocol and variance in measurement instruments, like microplate readers, contributes to the difficulty in comparing fluorescence measurements between instruments and different instances of measurement.

For the 2018 InterLab Study, the variability in fluorescence measurements when assessing a population of cells was investigated and the utility of normalizing measurements to absolute cell count or colony forming units (CFU) was assessed [1]. Team UAlberta accomplished these objectives this by following the InterLab Study protocols to measure the fluorescence and cellular density of eight devices which were calibrated against established standards.

Materials and Methods

The calibration and measurement procedures were performed by the members of Team UAlberta as outlined in the 2018 InterLab Study Protocols. To view the 2018 InterLab Study Protocols, click here. The following protocols describe Team UAlberta’s methods when none was specified by the InterLab protocols and to provide information about the measurement instruments.