Difference between revisions of "Team:Marburg/Improve"

Revision as of 00:10, 17 October 2018

Improve

There is always space for improvement, no matter how long you've been in the business.
-- Oscar de la Hoya

Cloning is the daily bread of molecular biologists. Almost all synbio projects start with weeks or months of cloning, a time which is the most frustrating and simultaneously least rewarding period of a project. Being able to distinguish between the native vector and the successfully assembled plasmid clearly while picking can save a lot of time and work. During the last decades, many approaches have been established to help distinguishing between false and correctly assembled plasmids. In our project, we created the Marburg Collection, a novel golden-gate-based cloning toolbox. This toolbox consists of LVL0 parts stored in a pSB1C3 derivat. As many plasmids had to be created, we desperately needed a simple, fast and cheap way to reliably select correct colonies even for inefficient clonings.

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-Cloning is the daily bread of molecular biologists. Almost all synbio projects start with weeks or months of cloning, a time which is the most frustrating and simultaneously least rewarding period of a project. Being able to distinguish between the native vector and the successfully assembled plasmid clearly while picking can save a lot of time and work. During the last decades, many approaches have been established to help distinguishing between false and correctly assembled plasmids. In our project, we created the Marburg Collection, a novel golden-gate-based cloning toolbox. This toolbox consists of LVL0 parts stored in a  <a href=" http://parts.igem.org/Part:pSB1C3 ">pSB1C3</a> derivat. As many plasmids had to be created, we desperately needed a simple, fast and cheap way to reliably select correct colonies even for inefficient clonings.
+Cloning is the daily bread of molecular biologists. Almost all synbio projects start with weeks or months of cloning, a time which is the most frustrating and simultaneously least rewarding period of a project. Being able to distinguish between the native vector and the successfully assembled plasmid clearly while picking can save a lot of time and work. During the last decades, many approaches have been established to help distinguishing between false and correctly assembled plasmids. In our project, we created the Marburg Collection, a novel golden-gate-based cloning toolbox. This toolbox consists of LVL0 parts stored in a  <a href=" http://parts.igem.org/Part:pSB1C3 ">
+		<abbr title=" Link to the  iGEM part registry " >pSB1C3</abbr></a>
+ derivat. As many plasmids had to be created, we desperately needed a simple, fast and cheap way to reliably select correct colonies even for inefficient clonings.